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The map label for this gene is greB [H]

Identifier: 209693811

GI number: 209693811

Start: 235883

End: 236350

Strand: Direct

Name: greB [H]

Synonym: VSAL_I0196

Alternate gene names: 209693811

Gene position: 235883-236350 (Clockwise)

Preceding gene: 209693810

Following gene: 209693812

Centisome position: 7.09

GC content: 44.02

Gene sequence:

>468_bases
ATGAAAACCAATTTAATCACACGTGAAGGCTTTAACAAGCTACAAGATGAACTCACGTTTCTATGGAAGGAAGATCGCCC
TGAAGTCACCAAAAAAGTGACTTGGGCGGCGAGCTTAGGTGATCGTAGCGAAAACGCTGATTACCAATACAATAAGAAAC
GCCTACGCGAGATTGACCGTCGAGTGCGCTATTTGCGTAAGCGTTTGGATCAAGTCAAAGTCGTTGATTACTCCCCCCAA
CAAGATGGCAAAGTCTTTTTCGGTGCGTTCGTTGTGATTGAAAACGAACAAGGCGACACCCTCTCTTTTCGTATTGTCGG
CCCTGATGAAATTTTTGGCCGCCATGATTACATTTCGATTGATTCTCCTATGGCGCGAGCGCTGATTAAGAAGGAAGTGG
ATGAAGAAGCGATTGTAAAAACGCCGGAGGGAGATAAAGAATGGTTTGTGAATTCGATAAAATACTAA

Upstream 100 bases:

>100_bases
GGCTGCATAAACGATTTAAAACCATGGAAGATCCAAGCATTAAGAAAGGCATTCGTTACGATTAATCGTATGCATTATTT
CGGAGTTAAACTAGGGAAAG

Downstream 100 bases:

>100_bases
GAACTATACGCTGCGCTAACTAGAACGCTTCGCTCTAGAAACTATAAAAGAAAAAGCATGAACCAATTAAAACGGTACTT
CCCAAATCAGCCATACATGC

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MKTNLITREGFNKLQDELTFLWKEDRPEVTKKVTWAASLGDRSENADYQYNKKRLREIDRRVRYLRKRLDQVKVVDYSPQ
QDGKVFFGAFVVIENEQGDTLSFRIVGPDEIFGRHDYISIDSPMARALIKKEVDEEAIVKTPEGDKEWFVNSIKY

Sequences:

>Translated_155_residues
MKTNLITREGFNKLQDELTFLWKEDRPEVTKKVTWAASLGDRSENADYQYNKKRLREIDRRVRYLRKRLDQVKVVDYSPQ
QDGKVFFGAFVVIENEQGDTLSFRIVGPDEIFGRHDYISIDSPMARALIKKEVDEEAIVKTPEGDKEWFVNSIKY
>Mature_155_residues
MKTNLITREGFNKLQDELTFLWKEDRPEVTKKVTWAASLGDRSENADYQYNKKRLREIDRRVRYLRKRLDQVKVVDYSPQ
QDGKVFFGAFVVIENEQGDTLSFRIVGPDEIFGRHDYISIDSPMARALIKKEVDEEAIVKTPEGDKEWFVNSIKY

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=155, Percent_Identity=74.8387096774194, Blast_Score=242, Evalue=8e-66,
Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=35.031847133758, Blast_Score=95, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18267; Mature: 18267

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNLITREGFNKLQDELTFLWKEDRPEVTKKVTWAASLGDRSENADYQYNKKRLREIDR
CCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHEEEHHHCCCCCCCCCCHHHHHHHHHHHH
RVRYLRKRLDQVKVVDYSPQQDGKVFFGAFVVIENEQGDTLSFRIVGPDEIFGRHDYISI
HHHHHHHHHHHEEEEECCCCCCCCEEEEEEEEEECCCCCEEEEEEECHHHHHCCCCEEEC
DSPMARALIKKEVDEEAIVKTPEGDKEWFVNSIKY
CCHHHHHHHHHHCCCHHEEECCCCCHHHHHHCCCC
>Mature Secondary Structure
MKTNLITREGFNKLQDELTFLWKEDRPEVTKKVTWAASLGDRSENADYQYNKKRLREIDR
CCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHEEEHHHCCCCCCCCCCHHHHHHHHHHHH
RVRYLRKRLDQVKVVDYSPQQDGKVFFGAFVVIENEQGDTLSFRIVGPDEIFGRHDYISI
HHHHHHHHHHHEEEEECCCCCCCCEEEEEEEEEECCCCCEEEEEEECHHHHHCCCCEEEC
DSPMARALIKKEVDEEAIVKTPEGDKEWFVNSIKY
CCHHHHHHHHHHCCCHHEEECCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]