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The map label for this gene is htrB [H]

Identifier: 209693810

GI number: 209693810

Start: 234906

End: 235847

Strand: Direct

Name: htrB [H]

Synonym: VSAL_I0195

Alternate gene names: 209693810

Gene position: 234906-235847 (Clockwise)

Preceding gene: 209693809

Following gene: 209693811

Centisome position: 7.06

GC content: 43.21

Gene sequence:

>942_bases
ATGATGTCTAAATATCAAGCCCCTACATTCTCTTACTCCCTTCTGCATCCTAAATATTGGGGGGTTTGGTTTGGCTTTGG
TTTATTGTTCACCATCGTCACTATTTTACCGTATAAAGCGACCTATAAACTCGCTCGTGGGTTAGGGTTTCTAGGATTGA
AAGTAAGTACGTCTCGCGCTCATGTCGCTCGTCGCAATCTTGAATTGGCTTTTCCTGAAATGACCCCTTCTGAGCGCGAA
GCCATCGTTATCGAAAACTTCAAAAACTCAGGGTTAGCAATATTCGAGACAGCTATGGCTTGGGTATGGCCAGATTGGCG
TATAGAAAAACACTTTAGCTACGAGAATGTTCAACATCTATTGGATTTTGAGAAAGAAAAAAAAGGCGTATTGGTCGTTA
TTGTTCACGCCCTCAACCTTGAGTTAACTTGTCGAGCAATGGCAGCTGTCTCTCCTGGCTACGGCGTTTATCGTCCTCAC
AACAACCCAGCTTATGACTTTATTCAGTACTGGGGCCGCACGAGTCACGGAAATCAAACCATCGATCGTAAAGACGTAAA
AGGCATGATTCGTAAGTTACGTAATGGCGAGCGAGTCTGTTATTTACCAGATCACGACTACAATCGAAATAAATCGGTGT
TTGTGCCTTTCTTTGCTGTTCCAGATGCGTGCACCACCATTGGCACTGATATTTTAATCAGTGCGAGTCAATGTGCGGTC
ATCACTGCATCAGGATTTAGAACCTACAATAATTATCATCTAAATATTGATCATGACATCAGCGACCAATTGCCGAAGAA
TGATCCCACTGGCGTTGCGACAGTAATGAACCAAGCCGTTGAGCGTGTCATTCTTCGTGGTATTCCTCAATGGATGTGGC
TGCATAAACGATTTAAAACCATGGAAGATCCAAGCATTAAGAAAGGCATTCGTTACGATTAA

Upstream 100 bases:

>100_bases
AATTTTAATGAGTATTGGGCACTATTAAGTGCTCAGATCCAATAAATCCAACCCAATCTAACCGCATTGCTCGTTAGATT
GGTGTTTTTCAAATAGGATT

Downstream 100 bases:

>100_bases
TCGTATGCATTATTTCGGAGTTAAACTAGGGAAAGATGAAAACCAATTTAATCACACGTGAAGGCTTTAACAAGCTACAA
GATGAACTCACGTTTCTATG

Product: lipid A biosynthesis lauroyl acyltransferase

Products: KDO2-(lauroyl)-lipid IVA [C]

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MMSKYQAPTFSYSLLHPKYWGVWFGFGLLFTIVTILPYKATYKLARGLGFLGLKVSTSRAHVARRNLELAFPEMTPSERE
AIVIENFKNSGLAIFETAMAWVWPDWRIEKHFSYENVQHLLDFEKEKKGVLVVIVHALNLELTCRAMAAVSPGYGVYRPH
NNPAYDFIQYWGRTSHGNQTIDRKDVKGMIRKLRNGERVCYLPDHDYNRNKSVFVPFFAVPDACTTIGTDILISASQCAV
ITASGFRTYNNYHLNIDHDISDQLPKNDPTGVATVMNQAVERVILRGIPQWMWLHKRFKTMEDPSIKKGIRYD

Sequences:

>Translated_313_residues
MMSKYQAPTFSYSLLHPKYWGVWFGFGLLFTIVTILPYKATYKLARGLGFLGLKVSTSRAHVARRNLELAFPEMTPSERE
AIVIENFKNSGLAIFETAMAWVWPDWRIEKHFSYENVQHLLDFEKEKKGVLVVIVHALNLELTCRAMAAVSPGYGVYRPH
NNPAYDFIQYWGRTSHGNQTIDRKDVKGMIRKLRNGERVCYLPDHDYNRNKSVFVPFFAVPDACTTIGTDILISASQCAV
ITASGFRTYNNYHLNIDHDISDQLPKNDPTGVATVMNQAVERVILRGIPQWMWLHKRFKTMEDPSIKKGIRYD
>Mature_313_residues
MMSKYQAPTFSYSLLHPKYWGVWFGFGLLFTIVTILPYKATYKLARGLGFLGLKVSTSRAHVARRNLELAFPEMTPSERE
AIVIENFKNSGLAIFETAMAWVWPDWRIEKHFSYENVQHLLDFEKEKKGVLVVIVHALNLELTCRAMAAVSPGYGVYRPH
NNPAYDFIQYWGRTSHGNQTIDRKDVKGMIRKLRNGERVCYLPDHDYNRNKSVFVPFFAVPDACTTIGTDILISASQCAV
ITASGFRTYNNYHLNIDHDISDQLPKNDPTGVATVMNQAVERVILRGIPQWMWLHKRFKTMEDPSIKKGIRYD

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=295, Percent_Identity=44.4067796610169, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI87082094, Length=293, Percent_Identity=39.2491467576792, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI1788161, Length=309, Percent_Identity=27.1844660194175, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 35987; Mature: 35987

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSKYQAPTFSYSLLHPKYWGVWFGFGLLFTIVTILPYKATYKLARGLGFLGLKVSTSRA
CCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEEECCHH
HVARRNLELAFPEMTPSEREAIVIENFKNSGLAIFETAMAWVWPDWRIEKHFSYENVQHL
HHHHCCCEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEECCCCHHHHHHH
LDFEKEKKGVLVVIVHALNLELTCRAMAAVSPGYGVYRPHNNPAYDFIQYWGRTSHGNQT
HHHHCCCCCEEEEEEEECCCEEEEEEHHHCCCCCCEECCCCCCHHHHHHHCCCCCCCCCC
IDRKDVKGMIRKLRNGERVCYLPDHDYNRNKSVFVPFFAVPDACTTIGTDILISASQCAV
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEEECCCHHHHHCCHHEEECCCEEE
ITASGFRTYNNYHLNIDHDISDQLPKNDPTGVATVMNQAVERVILRGIPQWMWLHKRFKT
EEECCCEEECCEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
MEDPSIKKGIRYD
CCCCCCCCCCCCC
>Mature Secondary Structure
MMSKYQAPTFSYSLLHPKYWGVWFGFGLLFTIVTILPYKATYKLARGLGFLGLKVSTSRA
CCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEEECCHH
HVARRNLELAFPEMTPSEREAIVIENFKNSGLAIFETAMAWVWPDWRIEKHFSYENVQHL
HHHHCCCEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEECCCCHHHHHHH
LDFEKEKKGVLVVIVHALNLELTCRAMAAVSPGYGVYRPHNNPAYDFIQYWGRTSHGNQT
HHHHCCCCCEEEEEEEECCCEEEEEEHHHCCCCCCEECCCCCCHHHHHHHCCCCCCCCCC
IDRKDVKGMIRKLRNGERVCYLPDHDYNRNKSVFVPFFAVPDACTTIGTDILISASQCAV
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEEECCCHHHHHCCHHEEECCCEEE
ITASGFRTYNNYHLNIDHDISDQLPKNDPTGVATVMNQAVERVILRGIPQWMWLHKRFKT
EEECCCEEECCEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
MEDPSIKKGIRYD
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO2-lipid IVA; lauroyl-ACP [C]

Specific reaction: KDO2-lipid IVA + lauroyl-ACP = KDO2-(lauroyl)-lipid IVA + [acyl carrier protein] [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]