Click here to switch to the map view.

The map label for this gene is recF

Identifier: 209693645

GI number: 209693645

Start: 9989

End: 11068

Strand: Direct

Name: recF

Synonym: VSAL_I0011

Alternate gene names: 209693645

Gene position: 9989-11068 (Clockwise)

Preceding gene: 209693644

Following gene: 209693646

Centisome position: 0.3

GC content: 41.11

Gene sequence:

>1080_bases
ATGCCGTTATCACGTTTAATTATTAATGATTTTCGCAATATTGAAACGTGTGATATTCAATTATCTACCGGCTTTAATTT
TGTTATCGGTCCTAATGGAAGTGGGAAAACCAGTGTCCTTGAAGCGATATACTTGCTCGGACACGGGCGTTCTTTTAAAA
GCTCGTTAACAGGTCGAATTATTCGAAATAGCTGTGACGAGCTTTTTATTCATGGTCGATTTACTACGCCAGAGCAATTT
GAATTGCCAATCGGCATAAATAAACAGAGAGATGGCACAACGGAAGTAAAAATAGGCGGAGAAAGTGGTCAAAAACTGGC
ACAATTAGCCAAGGTTTTACCACTGCAACTGATTCATCCTGAAGGGTTTGAGTTGGTAACTGATGGACCTAAGTTCCGTC
GTGCTTTTATTGATTGGGGAGTGTTTCACGTGGAACCTGCTTTTTATGAAGCATGGAGCCGAGTGAAACGGTTAACCAAA
CAACGAAATGCCTTATTAAAAACGGCACAAAGCTATCGAGAGTTGAGTTATTGGGATTTGGAACTTGCCAACCTTGCTGA
GAAGATAGATCAGTGGCGAGTGGATTACATCAATCACATATCTGAAGCAACTCAACAAATATGTCAGGCATTCTTACCAG
AATACGACATAAAGCTGTCATACTACCGTGGTTGGGAGAGAGAGACTCCTTATGCGGAATTGCTTAAACGAAACTTTGAG
CGAGATAAGCAGCTGGGTTATACCGTCGGCGGCCCAAATAAAGCCGACTTAAGAATTAAAGTTGCCGGTACTCCTGTTGA
AGATGTGCTGTCTAGAGGTCAACTAAAGTTGATGGTATGTGCGTTACGATTAGCACAAGGACAGCATTTAACTGAAGCAA
CAGGAAAACAGTGCATCTATTTAATAGACGATTTTGCTTCTGAACTGGATAGCCACCGCCGCCAATTGCTGGCTCAATAT
TTGAAACAAACAAAGGCACAGGTGTTTATTAGTTCAATTACTGCTGAACAGATTGCAGATATGCATGATGAAGAAAGCAA
GATGTTTGAGATAGAACATGGCAAAATAGCCCAAGGATAA

Upstream 100 bases:

>100_bases
TCGATGACAACAGCAAATGCGAGTGCATTAGTGGAAGATTGTGCGAACGATGAAGCACAATATGTTGTTATGCCAATTCG
TCTGTAAAAGACAAAAATAG

Downstream 100 bases:

>100_bases
AAACGCGAGAGTAACTCATGTCAGAAAATTATGATTCATCGAGTATTAAAGTACTAAAAGGTCTGGATGCGGTACGTAAG
CGTCCGGGTATGTATATCGG

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MPLSRLIINDFRNIETCDIQLSTGFNFVIGPNGSGKTSVLEAIYLLGHGRSFKSSLTGRIIRNSCDELFIHGRFTTPEQF
ELPIGINKQRDGTTEVKIGGESGQKLAQLAKVLPLQLIHPEGFELVTDGPKFRRAFIDWGVFHVEPAFYEAWSRVKRLTK
QRNALLKTAQSYRELSYWDLELANLAEKIDQWRVDYINHISEATQQICQAFLPEYDIKLSYYRGWERETPYAELLKRNFE
RDKQLGYTVGGPNKADLRIKVAGTPVEDVLSRGQLKLMVCALRLAQGQHLTEATGKQCIYLIDDFASELDSHRRQLLAQY
LKQTKAQVFISSITAEQIADMHDEESKMFEIEHGKIAQG

Sequences:

>Translated_359_residues
MPLSRLIINDFRNIETCDIQLSTGFNFVIGPNGSGKTSVLEAIYLLGHGRSFKSSLTGRIIRNSCDELFIHGRFTTPEQF
ELPIGINKQRDGTTEVKIGGESGQKLAQLAKVLPLQLIHPEGFELVTDGPKFRRAFIDWGVFHVEPAFYEAWSRVKRLTK
QRNALLKTAQSYRELSYWDLELANLAEKIDQWRVDYINHISEATQQICQAFLPEYDIKLSYYRGWERETPYAELLKRNFE
RDKQLGYTVGGPNKADLRIKVAGTPVEDVLSRGQLKLMVCALRLAQGQHLTEATGKQCIYLIDDFASELDSHRRQLLAQY
LKQTKAQVFISSITAEQIADMHDEESKMFEIEHGKIAQG
>Mature_358_residues
PLSRLIINDFRNIETCDIQLSTGFNFVIGPNGSGKTSVLEAIYLLGHGRSFKSSLTGRIIRNSCDELFIHGRFTTPEQFE
LPIGINKQRDGTTEVKIGGESGQKLAQLAKVLPLQLIHPEGFELVTDGPKFRRAFIDWGVFHVEPAFYEAWSRVKRLTKQ
RNALLKTAQSYRELSYWDLELANLAEKIDQWRVDYINHISEATQQICQAFLPEYDIKLSYYRGWERETPYAELLKRNFER
DKQLGYTVGGPNKADLRIKVAGTPVEDVLSRGQLKLMVCALRLAQGQHLTEATGKQCIYLIDDFASELDSHRRQLLAQYL
KQTKAQVFISSITAEQIADMHDEESKMFEIEHGKIAQG

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=356, Percent_Identity=58.4269662921348, Blast_Score=444, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_ALISL (B6EP48)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002261573.1
- GeneID:   6988419
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0011
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 40958; Mature: 40827

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSRLIINDFRNIETCDIQLSTGFNFVIGPNGSGKTSVLEAIYLLGHGRSFKSSLTGRI
CCHHHHHHHHCCCCEEEEEEEECCEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
IRNSCDELFIHGRFTTPEQFELPIGINKQRDGTTEVKIGGESGQKLAQLAKVLPLQLIHP
HHCCCCCEEEEECCCCCCCEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCEEECC
EGFELVTDGPKFRRAFIDWGVFHVEPAFYEAWSRVKRLTKQRNALLKTAQSYRELSYWDL
CCCEEEECCHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELANLAEKIDQWRVDYINHISEATQQICQAFLPEYDIKLSYYRGWERETPYAELLKRNFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHH
RDKQLGYTVGGPNKADLRIKVAGTPVEDVLSRGQLKLMVCALRLAQGQHLTEATGKQCIY
HHHHCCCEECCCCCCCEEEEEECCCHHHHHCCCCHHHHHHHHHHHCCCCHHHHCCCCEEE
LIDDFASELDSHRRQLLAQYLKQTKAQVFISSITAEQIADMHDEESKMFEIEHGKIAQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCC
>Mature Secondary Structure 
PLSRLIINDFRNIETCDIQLSTGFNFVIGPNGSGKTSVLEAIYLLGHGRSFKSSLTGRI
CHHHHHHHHCCCCEEEEEEEECCEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
IRNSCDELFIHGRFTTPEQFELPIGINKQRDGTTEVKIGGESGQKLAQLAKVLPLQLIHP
HHCCCCCEEEEECCCCCCCEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCEEECC
EGFELVTDGPKFRRAFIDWGVFHVEPAFYEAWSRVKRLTKQRNALLKTAQSYRELSYWDL
CCCEEEECCHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELANLAEKIDQWRVDYINHISEATQQICQAFLPEYDIKLSYYRGWERETPYAELLKRNFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHH
RDKQLGYTVGGPNKADLRIKVAGTPVEDVLSRGQLKLMVCALRLAQGQHLTEATGKQCIY
HHHHCCCEECCCCCCCEEEEEECCCHHHHHCCCCHHHHHHHHHHHCCCCHHHHCCCCEEE
LIDDFASELDSHRRQLLAQYLKQTKAQVFISSITAEQIADMHDEESKMFEIEHGKIAQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA