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The map label for this gene is gyrB [H]

Identifier: 209693646

GI number: 209693646

Start: 11086

End: 13503

Strand: Direct

Name: gyrB [H]

Synonym: VSAL_I0012

Alternate gene names: 209693646

Gene position: 11086-13503 (Clockwise)

Preceding gene: 209693645

Following gene: 209693653

Centisome position: 0.33

GC content: 42.43

Gene sequence:

>2418_bases
ATGTCAGAAAATTATGATTCATCGAGTATTAAAGTACTAAAAGGTCTGGATGCGGTACGTAAGCGTCCGGGTATGTATAT
CGGCGACACCGACGACGGTACTGGTCTTCACCACATGGTGTTTGAGGTTGTCGATAACTCAATCGATGAAGCTCTTGCTG
GTCACTGTAGCGACATTATTGTTACCATTCATGAAGATGACTCTGTCTCTGTAAGTGATGATGGTCGTGGTATACCAACT
GAAATTCACGAAGAAGAAGGCGTATCTGCTGCAGAAGTTATCATGACGGTACTTCACGCAGGTGGTAAGTTTGATGATAA
CTCATACAAAGTATCGGGTGGCCTTCACGGTGTTGGTGTTTCGGTTGTAAATGCATTATCAGAGAAAGTGGAGCTAACGA
TTAATCGTAGAGGCGAAATTTTCCAACAAATTTATTGTCATGGTGTTCCTCAGGCTCCATTAGCCGTCATTGGTACAACA
GATACAACGGGTACTAAAATCCGTTTTTGGCCTAGTGACCAAACGTTCGCAAATATTGAATTTGTGTATGACATTCTAGC
AAAACGCTTACGTGAGCTTTCTTTCTTAAACTCTGGCGTATCTATCCGTCTACAAGATATGCGTGAAGAAGATAAATCTG
ATCACTTCATGTTTGAAGGTGGCATCAAAGCGTTTGTTACTCACTTGAACCGTAATAAAACACCAGTACATCAGAAAATA
TTCTATTTTGATTCTGAGCGTAAAGAAGACGGTATTGTTGTAGAAGTAGCGATGCAGTGGAATGACTCTTACCAAGAGAA
CATTTTCTGTTATACGAATAACATCCCACAACGTGATGGCGGTGCTCACTTATCTGGCTTCCGTTCTGCGTTAACACGTA
CTTTGAACTCGTTCATGGACAAAGAAGGCTTTTCTAAGAAAGCAAAATCATCTGCGTCAGGTGATGATGCTCGTGAAGGC
CTAACTGCCGTTGTTTCTGTTAAAGTGCCTGATCCTAAGTTCTCAAGCCAAACTAAAGATAAACTGGTTTCTTCAGAAGT
GAAGTCAGCGGTTGAATCGGCAATGAACGAAAAACTGGCTGAATTCTTAGCGGAAAATCCAGGCGAAGCTAAAATGGTTT
GTTCTAAAATCATTGATGCGGCTCGTGCTCGTGAAGCGGCTCGTAAGGCTCGTGAAATGACTCGTCGTAAAGGCGCGTTA
GATATCGCTGGTCTTCCTGGTAAGCTGGCTGATTGTCAGGAAAAAGATCCTGCTCAATCTGAACTATACATAGTGGAAGG
GGACTCTGCTGGCGGTTCAGCCAAGCAGGGACGTAACCGTAAAAACCAAGCGATCCTTCCGCTTAAAGGTAAAATTCTAA
ACGTAGAAAAAGCACGTTTTGATAAAATGATCTCTTCTCAAGAGGTTGGTACGTTAATTACTGCACTAGGTTGTGGTATT
GGTCGTGACGAATACAATCCGGATAAATTGCGTTACCACAACATCATCATCATGACCGATGCCGATGTCGATGGTTCACA
CATCCGTACTTTGCTATTGACGTTCTTCTATCGTCAAATGCCAGAATTGATTGAACGTGGCTACATCTACATTGCTCAAC
CACCTCTTTACAAAGTAAAGAAAGGCAAACAAGAGCAATACATTAAAGATGAAGAAGCGATGGCGGAATATCAAATAACG
CAAGCATTAGACAATGCTTCGCTTTTTGTTAATCCTCACGAATCAATTTTTGGTCAAGAGCTTGAAAATTTAATTCGTAA
ATTTAACAGTGTAATGAAACTGCTTGATCGTATGAGTCGCCGTTACCCACTGCCATTGTTAAATCGTTTAGTCTACACAT
CACGTTTAACACAAGATGAGTGTGAAAACGAAGCGACAGTTCAAGCATGGACTGAAGCGTTAGTTATCGCATTGAATGAG
CATGAAACAGGTGCAAGCCAATACACCGCTGCGCTGCAGTTTAACGAAGAGTTAAACATTCACCAGCCGAAGCTTGTTGT
TCGTACTCACGGTGTTCAACATGAATACGTACTAAGTGCTGATTTGCTTAACTCGAAAGAGTACGGCCGTATTGCTGAAC
TGTCAGAATCACTGAATGGTCTGATTGAAGAAGGCGCTTACGTACAGCGTGGAGAGCGTAAACTAGCGACCGAAACGTTA
GATGAAGCCATTGCATGGTTAATTAAAGACTCTCGTCGTGGTGTGTACTTACAGCGTTACAAAGGTCTGGGTGAAATGAA
CCCTGAGCAGCTTTGGGAAACAACAATGGATCCTGATTCTCGCCGTATGATGCAAGTAACTATCAACGATGCGGTAAGTG
CGGATGAGTTGTTTACTACGCTAATGGGTGATCATGTTGAACCGCGTCGTAACTTCATTGAAGAAAACGCATTGCGCGTT
GCTAACTTAGACGTATAA

Upstream 100 bases:

>100_bases
TTACTGCTGAACAGATTGCAGATATGCATGATGAAGAAAGCAAGATGTTTGAGATAGAACATGGCAAAATAGCCCAAGGA
TAAAAACGCGAGAGTAACTC

Downstream 100 bases:

>100_bases
ATTGAGTAAATATTTGCTGTAATCACTGATTTCTGCGTCGAAGGTGCTCACTTATGTTCATAAGCTCCGCGCCCTCTCCT
TGAACTAAGCGATTACAGCG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 805; Mature: 804

Protein sequence:

>805_residues
MSENYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCSDIIVTIHEDDSVSVSDDGRGIPT
EIHEEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEKVELTINRRGEIFQQIYCHGVPQAPLAVIGTT
DTTGTKIRFWPSDQTFANIEFVYDILAKRLRELSFLNSGVSIRLQDMREEDKSDHFMFEGGIKAFVTHLNRNKTPVHQKI
FYFDSERKEDGIVVEVAMQWNDSYQENIFCYTNNIPQRDGGAHLSGFRSALTRTLNSFMDKEGFSKKAKSSASGDDAREG
LTAVVSVKVPDPKFSSQTKDKLVSSEVKSAVESAMNEKLAEFLAENPGEAKMVCSKIIDAARAREAARKAREMTRRKGAL
DIAGLPGKLADCQEKDPAQSELYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMISSQEVGTLITALGCGI
GRDEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIAQPPLYKVKKGKQEQYIKDEEAMAEYQIT
QALDNASLFVNPHESIFGQELENLIRKFNSVMKLLDRMSRRYPLPLLNRLVYTSRLTQDECENEATVQAWTEALVIALNE
HETGASQYTAALQFNEELNIHQPKLVVRTHGVQHEYVLSADLLNSKEYGRIAELSESLNGLIEEGAYVQRGERKLATETL
DEAIAWLIKDSRRGVYLQRYKGLGEMNPEQLWETTMDPDSRRMMQVTINDAVSADELFTTLMGDHVEPRRNFIEENALRV
ANLDV

Sequences:

>Translated_805_residues
MSENYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCSDIIVTIHEDDSVSVSDDGRGIPT
EIHEEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEKVELTINRRGEIFQQIYCHGVPQAPLAVIGTT
DTTGTKIRFWPSDQTFANIEFVYDILAKRLRELSFLNSGVSIRLQDMREEDKSDHFMFEGGIKAFVTHLNRNKTPVHQKI
FYFDSERKEDGIVVEVAMQWNDSYQENIFCYTNNIPQRDGGAHLSGFRSALTRTLNSFMDKEGFSKKAKSSASGDDAREG
LTAVVSVKVPDPKFSSQTKDKLVSSEVKSAVESAMNEKLAEFLAENPGEAKMVCSKIIDAARAREAARKAREMTRRKGAL
DIAGLPGKLADCQEKDPAQSELYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMISSQEVGTLITALGCGI
GRDEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIAQPPLYKVKKGKQEQYIKDEEAMAEYQIT
QALDNASLFVNPHESIFGQELENLIRKFNSVMKLLDRMSRRYPLPLLNRLVYTSRLTQDECENEATVQAWTEALVIALNE
HETGASQYTAALQFNEELNIHQPKLVVRTHGVQHEYVLSADLLNSKEYGRIAELSESLNGLIEEGAYVQRGERKLATETL
DEAIAWLIKDSRRGVYLQRYKGLGEMNPEQLWETTMDPDSRRMMQVTINDAVSADELFTTLMGDHVEPRRNFIEENALRV
ANLDV
>Mature_804_residues
SENYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCSDIIVTIHEDDSVSVSDDGRGIPTE
IHEEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSEKVELTINRRGEIFQQIYCHGVPQAPLAVIGTTD
TTGTKIRFWPSDQTFANIEFVYDILAKRLRELSFLNSGVSIRLQDMREEDKSDHFMFEGGIKAFVTHLNRNKTPVHQKIF
YFDSERKEDGIVVEVAMQWNDSYQENIFCYTNNIPQRDGGAHLSGFRSALTRTLNSFMDKEGFSKKAKSSASGDDAREGL
TAVVSVKVPDPKFSSQTKDKLVSSEVKSAVESAMNEKLAEFLAENPGEAKMVCSKIIDAARAREAARKAREMTRRKGALD
IAGLPGKLADCQEKDPAQSELYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMISSQEVGTLITALGCGIG
RDEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIAQPPLYKVKKGKQEQYIKDEEAMAEYQITQ
ALDNASLFVNPHESIFGQELENLIRKFNSVMKLLDRMSRRYPLPLLNRLVYTSRLTQDECENEATVQAWTEALVIALNEH
ETGASQYTAALQFNEELNIHQPKLVVRTHGVQHEYVLSADLLNSKEYGRIAELSESLNGLIEEGAYVQRGERKLATETLD
EAIAWLIKDSRRGVYLQRYKGLGEMNPEQLWETTMDPDSRRMMQVTINDAVSADELFTTLMGDHVEPRRNFIEENALRVA
NLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=553, Percent_Identity=25.6781193490054, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI19913406, Length=565, Percent_Identity=25.8407079646018, Blast_Score=128, Evalue=2e-29,
Organism=Escherichia coli, GI48994957, Length=807, Percent_Identity=73.4820322180917, Blast_Score=1215, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=555, Percent_Identity=38.7387387387387, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17535065, Length=540, Percent_Identity=26.2962962962963, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI212645845, Length=528, Percent_Identity=25.3787878787879, Blast_Score=130, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI212645657, Length=153, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=591, Percent_Identity=25.0423011844332, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17136538, Length=530, Percent_Identity=25.6603773584906, Blast_Score=132, Evalue=7e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 90148; Mature: 90017

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCSDII
CCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEE
VTIHEDDSVSVSDDGRGIPTEIHEEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGV
EEEECCCCEEECCCCCCCCCCCCHHCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
SVVNALSEKVELTINRRGEIFQQIYCHGVPQAPLAVIGTTDTTGTKIRFWPSDQTFANIE
HHHHHHHHHEEEEECCHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEECCCCCCHHHHH
FVYDILAKRLRELSFLNSGVSIRLQDMREEDKSDHFMFEGGIKAFVTHLNRNKTPVHQKI
HHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCCCEEEECCHHHHHHHHCCCCCCCHHEE
FYFDSERKEDGIVVEVAMQWNDSYQENIFCYTNNIPQRDGGAHLSGFRSALTRTLNSFMD
EEECCCCCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
KEGFSKKAKSSASGDDAREGLTAVVSVKVPDPKFSSQTKDKLVSSEVKSAVESAMNEKLA
HCCCCHHHCCCCCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EFLAENPGEAKMVCSKIIDAARAREAARKAREMTRRKGALDIAGLPGKLADCQEKDPAQS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCC
ELYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMISSQEVGTLITALGCGI
EEEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCC
GRDEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIAQPPLYKVK
CCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHH
KGKQEQYIKDEEAMAEYQITQALDNASLFVNPHESIFGQELENLIRKFNSVMKLLDRMSR
CCCHHHHCCHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RYPLPLLNRLVYTSRLTQDECENEATVQAWTEALVIALNEHETGASQYTAALQFNEELNI
CCCHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHEEEECCCCCCHHHEEEEEEECCCCCC
HQPKLVVRTHGVQHEYVLSADLLNSKEYGRIAELSESLNGLIEEGAYVQRGERKLATETL
CCCEEEEEECCCCEEEEEEHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHH
DEAIAWLIKDSRRGVYLQRYKGLGEMNPEQLWETTMDPDSRRMMQVTINDAVSADELFTT
HHHHHHHHHCCCCCEEHHHHCCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHH
LMGDHVEPRRNFIEENALRVANLDV
HHCCCCCHHHHHHHHCCEEEECCCC
>Mature Secondary Structure 
SENYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCSDII
CCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEE
VTIHEDDSVSVSDDGRGIPTEIHEEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGV
EEEECCCCEEECCCCCCCCCCCCHHCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
SVVNALSEKVELTINRRGEIFQQIYCHGVPQAPLAVIGTTDTTGTKIRFWPSDQTFANIE
HHHHHHHHHEEEEECCHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEECCCCCCHHHHH
FVYDILAKRLRELSFLNSGVSIRLQDMREEDKSDHFMFEGGIKAFVTHLNRNKTPVHQKI
HHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCCCEEEECCHHHHHHHHCCCCCCCHHEE
FYFDSERKEDGIVVEVAMQWNDSYQENIFCYTNNIPQRDGGAHLSGFRSALTRTLNSFMD
EEECCCCCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
KEGFSKKAKSSASGDDAREGLTAVVSVKVPDPKFSSQTKDKLVSSEVKSAVESAMNEKLA
HCCCCHHHCCCCCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EFLAENPGEAKMVCSKIIDAARAREAARKAREMTRRKGALDIAGLPGKLADCQEKDPAQS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCC
ELYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMISSQEVGTLITALGCGI
EEEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCC
GRDEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIAQPPLYKVK
CCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHH
KGKQEQYIKDEEAMAEYQITQALDNASLFVNPHESIFGQELENLIRKFNSVMKLLDRMSR
CCCHHHHCCHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RYPLPLLNRLVYTSRLTQDECENEATVQAWTEALVIALNEHETGASQYTAALQFNEELNI
CCCHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHEEEECCCCCCHHHEEEEEEECCCCCC
HQPKLVVRTHGVQHEYVLSADLLNSKEYGRIAELSESLNGLIEEGAYVQRGERKLATETL
CCCEEEEEECCCCEEEEEEHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHH
DEAIAWLIKDSRRGVYLQRYKGLGEMNPEQLWETTMDPDSRRMMQVTINDAVSADELFTT
HHHHHHHHHCCCCCEEHHHHCCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHH
LMGDHVEPRRNFIEENALRVANLDV
HHCCCCCHHHHHHHHCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739; 9464408 [H]