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The map label for this gene is dnaN [H]

Identifier: 209693644

GI number: 209693644

Start: 8866

End: 9975

Strand: Direct

Name: dnaN [H]

Synonym: VSAL_I0010

Alternate gene names: 209693644

Gene position: 8866-9975 (Clockwise)

Preceding gene: 209693643

Following gene: 209693645

Centisome position: 0.27

GC content: 40.36

Gene sequence:

>1110_bases
ATGAAATTTTCTATTGAACGCAGCCATTTATTAAAACCGCTGCAACAAGTATCTGGAGCCTTAGGTGGCCGTCCATCATT
GCCTATTTTTGGTAATATTTTGCTTCAAGTTGAAAATGGTCAACTGTCGATGACAGCAACAGATCTTGAAGTAGAACTTA
TTAGTAATATTCTGTTAGAAGGGGATTTTGAAGCGGGTGCGATTACGGTGCCTTCTCGTAAGTTCTTAGATATCTGTCGT
GGTTTACCTGACGGTGCAAATATTACTATTGTTTTAGAAGGTGATCGTGTTTTAGTTCGTTCTGGACGTAGCCGATTTAC
ATTAGCAACCTTACCAGCTCAAGATTTTCCGAATATAGAAGGCTGGCAAAGTGAAGTTGAAATTACGCTTTCTCAAGCAC
AACTTCGTAAGCTAATTGAAAGTACGCAATTCTCAATGGCAAACCAAGATGTTCGTTATTACCTTAATGGTATGATGTTT
GAAACTCAGGGGACTATTTTTCGCTCCGTTGCGACAGATGGACACCGAATGGCAGTATGTAACATGCCAGTAGAGTCAGA
ACTTCCTGAGCAACAAGTGATCATGCCACGCAAAGGTATTCAAGAGCTAGTACGCCTTCTTGACTCGCCAGAACAGAGTG
TTACGTTACAGTTTGGTAATTCAAATGTGCGGGCTGAGGTGAATAACTTTATCTTCACTTCTAAGCTTGTTGATGGTCGT
TTTCCTGATTATCGACGTGTAATGCCGCAAAGCAGTAATAAAACACTAGAAGCCGATTGTGACGAATTGCGTCAAGCGTT
TGCGCGTGCTGCTATTTTATCGAATGAAAAATTCCGAGGTGTTCGTGTTAATTTGGCAAACAACCAAATGCGTATTACAG
CGAACAACCCAGAGCAAGAAGAAGCAGAAGAAATCTTAGATGTCGATTTCCAAGGTGAAGACATTGAAATTGGTTTTAAC
GTTAGCTATGTACTAGATGTACTAGATGTACTTAATACGTTGAAATGTGAACGAGTACGAATTTCGATGACAACAGCAAA
TGCGAGTGCATTAGTGGAAGATTGTGCGAACGATGAAGCACAATATGTTGTTATGCCAATTCGTCTGTAA

Upstream 100 bases:

>100_bases
AGTTACGCGAAGAAAGCCATGATATTAAAGAAGATTACTCAAATCTGATCAGAACATTATCATCGTAAGCTATTTATCTA
CTTTTATATAAACGAGTATT

Downstream 100 bases:

>100_bases
AAGACAAAAATAGATGCCGTTATCACGTTTAATTATTAATGATTTTCGCAATATTGAAACGTGTGATATTCAATTATCTA
CCGGCTTTAATTTTGTTATC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MKFSIERSHLLKPLQQVSGALGGRPSLPIFGNILLQVENGQLSMTATDLEVELISNILLEGDFEAGAITVPSRKFLDICR
GLPDGANITIVLEGDRVLVRSGRSRFTLATLPAQDFPNIEGWQSEVEITLSQAQLRKLIESTQFSMANQDVRYYLNGMMF
ETQGTIFRSVATDGHRMAVCNMPVESELPEQQVIMPRKGIQELVRLLDSPEQSVTLQFGNSNVRAEVNNFIFTSKLVDGR
FPDYRRVMPQSSNKTLEADCDELRQAFARAAILSNEKFRGVRVNLANNQMRITANNPEQEEAEEILDVDFQGEDIEIGFN
VSYVLDVLDVLNTLKCERVRISMTTANASALVEDCANDEAQYVVMPIRL

Sequences:

>Translated_369_residues
MKFSIERSHLLKPLQQVSGALGGRPSLPIFGNILLQVENGQLSMTATDLEVELISNILLEGDFEAGAITVPSRKFLDICR
GLPDGANITIVLEGDRVLVRSGRSRFTLATLPAQDFPNIEGWQSEVEITLSQAQLRKLIESTQFSMANQDVRYYLNGMMF
ETQGTIFRSVATDGHRMAVCNMPVESELPEQQVIMPRKGIQELVRLLDSPEQSVTLQFGNSNVRAEVNNFIFTSKLVDGR
FPDYRRVMPQSSNKTLEADCDELRQAFARAAILSNEKFRGVRVNLANNQMRITANNPEQEEAEEILDVDFQGEDIEIGFN
VSYVLDVLDVLNTLKCERVRISMTTANASALVEDCANDEAQYVVMPIRL
>Mature_369_residues
MKFSIERSHLLKPLQQVSGALGGRPSLPIFGNILLQVENGQLSMTATDLEVELISNILLEGDFEAGAITVPSRKFLDICR
GLPDGANITIVLEGDRVLVRSGRSRFTLATLPAQDFPNIEGWQSEVEITLSQAQLRKLIESTQFSMANQDVRYYLNGMMF
ETQGTIFRSVATDGHRMAVCNMPVESELPEQQVIMPRKGIQELVRLLDSPEQSVTLQFGNSNVRAEVNNFIFTSKLVDGR
FPDYRRVMPQSSNKTLEADCDELRQAFARAAILSNEKFRGVRVNLANNQMRITANNPEQEEAEEILDVDFQGEDIEIGFN
VSYVLDVLDVLNTLKCERVRISMTTANASALVEDCANDEAQYVVMPIRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=369, Percent_Identity=65.8536585365854, Blast_Score=523, Evalue=1e-150,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41268; Mature: 41268

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSIERSHLLKPLQQVSGALGGRPSLPIFGNILLQVENGQLSMTATDLEVELISNILLE
CCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCEEEEEEECHHHHHHHHHHCC
GDFEAGAITVPSRKFLDICRGLPDGANITIVLEGDRVLVRSGRSRFTLATLPAQDFPNIE
CCCCCCEEECCCHHHHHHHCCCCCCCCEEEEEECCEEEEECCCCEEEEEECCCCCCCCCC
GWQSEVEITLSQAQLRKLIESTQFSMANQDVRYYLNGMMFETQGTIFRSVATDGHRMAVC
CCCCCEEEEECHHHHHHHHHHHHHHHCCCHHHHHCCCEEEECCCCEEEECCCCCCEEEEE
NMPVESELPEQQVIMPRKGIQELVRLLDSPEQSVTLQFGNSNVRAEVNNFIFTSKLVDGR
CCCCCCCCCCHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEECCCEEEEEHHHCCC
FPDYRRVMPQSSNKTLEADCDELRQAFARAAILSNEKFRGVRVNLANNQMRITANNPEQE
CCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEECCCCHH
EAEEILDVDFQGEDIEIGFNVSYVLDVLDVLNTLKCERVRISMTTANASALVEDCANDEA
HHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHCCCCC
QYVVMPIRL
EEEEEEEEC
>Mature Secondary Structure
MKFSIERSHLLKPLQQVSGALGGRPSLPIFGNILLQVENGQLSMTATDLEVELISNILLE
CCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCEEEEEEECHHHHHHHHHHCC
GDFEAGAITVPSRKFLDICRGLPDGANITIVLEGDRVLVRSGRSRFTLATLPAQDFPNIE
CCCCCCEEECCCHHHHHHHCCCCCCCCEEEEEECCEEEEECCCCEEEEEECCCCCCCCCC
GWQSEVEITLSQAQLRKLIESTQFSMANQDVRYYLNGMMFETQGTIFRSVATDGHRMAVC
CCCCCEEEEECHHHHHHHHHHHHHHHCCCHHHHHCCCEEEECCCCEEEECCCCCCEEEEE
NMPVESELPEQQVIMPRKGIQELVRLLDSPEQSVTLQFGNSNVRAEVNNFIFTSKLVDGR
CCCCCCCCCCHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEECCCEEEEEHHHCCC
FPDYRRVMPQSSNKTLEADCDELRQAFARAAILSNEKFRGVRVNLANNQMRITANNPEQE
CCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEECCCCHH
EAEEILDVDFQGEDIEIGFNVSYVLDVLDVLNTLKCERVRISMTTANASALVEDCANDEA
HHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHCCCCC
QYVVMPIRL
EEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]