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The map label for this gene is fruA-2 [H]

Identifier: 203284529

GI number: 203284529

Start: 672420

End: 674279

Strand: Reverse

Name: fruA-2 [H]

Synonym: BDU_633

Alternate gene names: 203284529

Gene position: 674279-672420 (Counterclockwise)

Preceding gene: 203284531

Following gene: 203284527

Centisome position: 72.37

GC content: 31.88

Gene sequence:

>1860_bases
ATGCAAGATTTATTTTCTAAAAAATTAATATTATTGAATTACGAAGCCAAAAGCAAAGATGACATAATTGAAAAAATGGC
CGACATGCTAAATGAAAATGGATACCTAAGAAACAAAGAAAATTTTATAAAAGACATTAAAAAACGAGAAGAAATAAGCG
GAACGGGTCTTGAAGAATATATTGCAATGCCCCATGCAAAAGGCAATTTTGTTGAAAAACATGGAATAGCAATATTAAGG
GTTAAAGACAAAGGATTTGACTTTGGTGCTGCTGACTTAAAACCCTCAAAACTATTTTTTATGATAGCTGTTCCTGAAAA
TACAACTGGTAATGACCATATTAAAACAATTTCTTATCTTAATAACATATTTAATAACGACATCCTAAGACAAGAAATTA
TGAATACAAACGACAAAAATAGATTTTTAGAAATTATTTTAAATACTCCTAACAGTGATACAATAATGAATAAAACGAAT
CTTGAAAATTTTATTTTAGCAGTAACTGCATGTCCCACAGGAATTGCCCATACATATATGGCAGCCGATAGCTTAAAAAG
AGCAGCAAACGAATTAAACGTCGAAATTAAAATAGAAACTAATGGCTCTAGTGGAATTGAAAATCCAATAAACGAAGAAG
ATATCAAAAAAGCAAAAGGAATCATTATTGCATCCGGTAAAGCCGTTAATAAAACAAGATTTAATGGCAAACCACTAATT
GAAGTAGGGGTTAAAGACGGTATACATAAGGCAAAAGAACTCATTCAAGATATCATCAATAACAAAGCAAAAATCTACAG
AAGCAACACATCACAAAAAACAGAAAGCTCAATAACAAATAATACAAGTAATACGGAAATATATAAACACTTAATGAATG
GTGTATCTTTCATGCTTCCATTTGTAGTATCTGGAGGAATAATAATTGCAATATCATTCATGTTTGGTATTAAAGCTTTT
GACCCAACCGATCCAAATTACAATAAAATAGCAGATATTTTAATGCAAATTGGTGGTGGTAATGCTTTCTTCCTAATGAT
TCCAATACTTGCTGGCTACATTTCATTTAGTATAGCAGAACGCCCTGGCCTTGCACCTGGAATGATTACAGGACTAATGA
TGAGTAAAGGAAATGCAGGATTTCTAGGGGGAATATTAGCAGGGTTTATTGCAGGATATGTCACATTAACAATAAAATCA
ATAAGTCAAAAAATAATACCAAAAAACATAAGCAGCATTAATCCTGTCTTAACTTATCCATTTTTCTCAGTACTAATAGC
TGGATTTTTAACATATATATTATTAGCACCAATTGCATATCTTAATTCATCTATAACAAATATATTAAATTCACTAAGTG
GAACAAATATGGCATTACTAGGTGCATTACTTGGAGGAATGATGGCAATAGATATGGGTGGACCGGTAAACAAGGCAGCT
TATGCATTTGGAATTGCAATGATAACCGCAGAAAACTATATTCCTCACGCAAGCGTAATGGCAGGAGGAATGATTCCCCC
AATAGGAATTGCACTTGCAACCAGCATTTTTAAGAACAAGTTTTCACAAGAAGAACATGAAGCTGGCAAAGTTTGTTATG
TTTTAGGAGCATGCTTTATAACAGAAGCCGTAATTCCTTTTGCAGCATCAGATCCTCTAAAAGTAATACCTGCTTGCATA
ATAGGTTCATCTATTGGAGGTTTTCTATCTGCATTATTTCAAGTAAAACTTATGGCTCCACATGGAGGTATTTTTATTCT
ACCAATAATAACTAATCCATTAATGTGGATAGTATCAATCCTAATTGGAAGTTTAATAACAGGAGTATTAATAGGATTTA
TGAAAAGAGATAAGAAATAA

Upstream 100 bases:

>100_bases
ATACAATTAAACAACTTAACATATTATAATTATATGATATAATTGTAATATATAATTTCTATTATAATAGGAATTATACA
CTATATCAAGGAGCTTCCCT

Downstream 100 bases:

>100_bases
TTCTTATCTCTCATTTATATTTGAAATAAACATTTCAACAAAGTTAATAAACTAATCTCAACTTTATTGGTTTTTGAAGA
CTTACAGTCTTAATCATATC

Product: PTS system, fructose-specific IIABC component

Products: NA

Alternate protein names: EIIABC-Fru; Fructose-specific phosphotransferase enzyme IIA component; EII-Fru; PTS system fructose-specific EIIA component; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MQDLFSKKLILLNYEAKSKDDIIEKMADMLNENGYLRNKENFIKDIKKREEISGTGLEEYIAMPHAKGNFVEKHGIAILR
VKDKGFDFGAADLKPSKLFFMIAVPENTTGNDHIKTISYLNNIFNNDILRQEIMNTNDKNRFLEIILNTPNSDTIMNKTN
LENFILAVTACPTGIAHTYMAADSLKRAANELNVEIKIETNGSSGIENPINEEDIKKAKGIIIASGKAVNKTRFNGKPLI
EVGVKDGIHKAKELIQDIINNKAKIYRSNTSQKTESSITNNTSNTEIYKHLMNGVSFMLPFVVSGGIIIAISFMFGIKAF
DPTDPNYNKIADILMQIGGGNAFFLMIPILAGYISFSIAERPGLAPGMITGLMMSKGNAGFLGGILAGFIAGYVTLTIKS
ISQKIIPKNISSINPVLTYPFFSVLIAGFLTYILLAPIAYLNSSITNILNSLSGTNMALLGALLGGMMAIDMGGPVNKAA
YAFGIAMITAENYIPHASVMAGGMIPPIGIALATSIFKNKFSQEEHEAGKVCYVLGACFITEAVIPFAASDPLKVIPACI
IGSSIGGFLSALFQVKLMAPHGGIFILPIITNPLMWIVSILIGSLITGVLIGFMKRDKK

Sequences:

>Translated_619_residues
MQDLFSKKLILLNYEAKSKDDIIEKMADMLNENGYLRNKENFIKDIKKREEISGTGLEEYIAMPHAKGNFVEKHGIAILR
VKDKGFDFGAADLKPSKLFFMIAVPENTTGNDHIKTISYLNNIFNNDILRQEIMNTNDKNRFLEIILNTPNSDTIMNKTN
LENFILAVTACPTGIAHTYMAADSLKRAANELNVEIKIETNGSSGIENPINEEDIKKAKGIIIASGKAVNKTRFNGKPLI
EVGVKDGIHKAKELIQDIINNKAKIYRSNTSQKTESSITNNTSNTEIYKHLMNGVSFMLPFVVSGGIIIAISFMFGIKAF
DPTDPNYNKIADILMQIGGGNAFFLMIPILAGYISFSIAERPGLAPGMITGLMMSKGNAGFLGGILAGFIAGYVTLTIKS
ISQKIIPKNISSINPVLTYPFFSVLIAGFLTYILLAPIAYLNSSITNILNSLSGTNMALLGALLGGMMAIDMGGPVNKAA
YAFGIAMITAENYIPHASVMAGGMIPPIGIALATSIFKNKFSQEEHEAGKVCYVLGACFITEAVIPFAASDPLKVIPACI
IGSSIGGFLSALFQVKLMAPHGGIFILPIITNPLMWIVSILIGSLITGVLIGFMKRDKK
>Mature_619_residues
MQDLFSKKLILLNYEAKSKDDIIEKMADMLNENGYLRNKENFIKDIKKREEISGTGLEEYIAMPHAKGNFVEKHGIAILR
VKDKGFDFGAADLKPSKLFFMIAVPENTTGNDHIKTISYLNNIFNNDILRQEIMNTNDKNRFLEIILNTPNSDTIMNKTN
LENFILAVTACPTGIAHTYMAADSLKRAANELNVEIKIETNGSSGIENPINEEDIKKAKGIIIASGKAVNKTRFNGKPLI
EVGVKDGIHKAKELIQDIINNKAKIYRSNTSQKTESSITNNTSNTEIYKHLMNGVSFMLPFVVSGGIIIAISFMFGIKAF
DPTDPNYNKIADILMQIGGGNAFFLMIPILAGYISFSIAERPGLAPGMITGLMMSKGNAGFLGGILAGFIAGYVTLTIKS
ISQKIIPKNISSINPVLTYPFFSVLIAGFLTYILLAPIAYLNSSITNILNSLSGTNMALLGALLGGMMAIDMGGPVNKAA
YAFGIAMITAENYIPHASVMAGGMIPPIGIALATSIFKNKFSQEEHEAGKVCYVLGACFITEAVIPFAASDPLKVIPACI
IGSSIGGFLSALFQVKLMAPHGGIFILPIITNPLMWIVSILIGSLITGVLIGFMKRDKK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=459, Percent_Identity=44.2265795206972, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1786951, Length=633, Percent_Identity=30.6477093206951, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI87082348, Length=437, Percent_Identity=32.2654462242563, Blast_Score=233, Evalue=3e-62,
Organism=Escherichia coli, GI1790386, Length=313, Percent_Identity=41.2140575079872, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1788729, Length=374, Percent_Identity=26.7379679144385, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1790387, Length=95, Percent_Identity=41.0526315789474, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI1788730, Length=96, Percent_Identity=40.625, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 67120; Mature: 67120

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS51094 PTS_EIIA_TYPE_2 ; PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDLFSKKLILLNYEAKSKDDIIEKMADMLNENGYLRNKENFIKDIKKREEISGTGLEEY
CCCHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
IAMPHAKGNFVEKHGIAILRVKDKGFDFGAADLKPSKLFFMIAVPENTTGNDHIKTISYL
HHCCCCCCCCCHHCCEEEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHH
NNIFNNDILRQEIMNTNDKNRFLEIILNTPNSDTIMNKTNLENFILAVTACPTGIAHTYM
HHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEECCCCCCCEEEEEEECCCHHHHHHH
AADSLKRAANELNVEIKIETNGSSGIENPINEEDIKKAKGIIIASGKAVNKTRFNGKPLI
HHHHHHHHHHHCEEEEEEEECCCCCCCCCCCHHHHHHHCCEEEECCCCCCCCCCCCCCEE
EVGVKDGIHKAKELIQDIINNKAKIYRSNTSQKTESSITNNTSNTEIYKHLMNGVSFMLP
EECCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
FVVSGGIIIAISFMFGIKAFDPTDPNYNKIADILMQIGGGNAFFLMIPILAGYISFSIAE
HHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHC
RPGLAPGMITGLMMSKGNAGFLGGILAGFIAGYVTLTIKSISQKIIPKNISSINPVLTYP
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHH
FFSVLIAGFLTYILLAPIAYLNSSITNILNSLSGTNMALLGALLGGMMAIDMGGPVNKAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCCCCHHH
YAFGIAMITAENYIPHASVMAGGMIPPIGIALATSIFKNKFSQEEHEAGKVCYVLGACFI
HHHHHHEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHH
TEAVIPFAASDPLKVIPACIIGSSIGGFLSALFQVKLMAPHGGIFILPIITNPLMWIVSI
HHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHHEEEECCCCCEEEEHHHHCHHHHHHHH
LIGSLITGVLIGFMKRDKK
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQDLFSKKLILLNYEAKSKDDIIEKMADMLNENGYLRNKENFIKDIKKREEISGTGLEEY
CCCHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
IAMPHAKGNFVEKHGIAILRVKDKGFDFGAADLKPSKLFFMIAVPENTTGNDHIKTISYL
HHCCCCCCCCCHHCCEEEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHH
NNIFNNDILRQEIMNTNDKNRFLEIILNTPNSDTIMNKTNLENFILAVTACPTGIAHTYM
HHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEECCCCCCCEEEEEEECCCHHHHHHH
AADSLKRAANELNVEIKIETNGSSGIENPINEEDIKKAKGIIIASGKAVNKTRFNGKPLI
HHHHHHHHHHHCEEEEEEEECCCCCCCCCCCHHHHHHHCCEEEECCCCCCCCCCCCCCEE
EVGVKDGIHKAKELIQDIINNKAKIYRSNTSQKTESSITNNTSNTEIYKHLMNGVSFMLP
EECCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
FVVSGGIIIAISFMFGIKAFDPTDPNYNKIADILMQIGGGNAFFLMIPILAGYISFSIAE
HHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHC
RPGLAPGMITGLMMSKGNAGFLGGILAGFIAGYVTLTIKSISQKIIPKNISSINPVLTYP
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHH
FFSVLIAGFLTYILLAPIAYLNSSITNILNSLSGTNMALLGALLGGMMAIDMGGPVNKAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCCCCHHH
YAFGIAMITAENYIPHASVMAGGMIPPIGIALATSIFKNKFSQEEHEAGKVCYVLGACFI
HHHHHHEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHH
TEAVIPFAASDPLKVIPACIIGSSIGGFLSALFQVKLMAPHGGIFILPIITNPLMWIVSI
HHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHHEEEECCCCCEEEEHHHHCHHHHHHHH
LIGSLITGVLIGFMKRDKK
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]