Click here to switch to the map view.

The map label for this gene is ksgA [H]

Identifier: 203284494

GI number: 203284494

Start: 623729

End: 624553

Strand: Reverse

Name: ksgA [H]

Synonym: BDU_591

Alternate gene names: 203284494

Gene position: 624553-623729 (Counterclockwise)

Preceding gene: 203284495

Following gene: 203284492

Centisome position: 67.04

GC content: 24.48

Gene sequence:

>825_bases
ATGAATATAAATTATAACAGCATAAACAGCATAAAGAATGTGTTAAAAAATAAAAATATAGCACCAAGAAAGATATGGGG
ACAAAATTATTTGATTAATGAAAACATAAGAGAAAAAATAATAGATGCACTTGACATTAAAAAAAATGAAAAAATTTGGG
AAATTGGTCCAGGACTTGGAGCAATGACAGCGACCTTATTAAATAAAGCAAATTTTTTAACGGCATTCGAAATTGACCCT
AAATATTCAGAAATCCTAAATGAAAAATTTGGTCAACTGAAAAATTTTAAACTAATTGAAGGTGACTTCTTAAAAACATA
TAAGCAAGAAAAAACAAGCATCAATAAAATAGTTTCAAATTTACCTTATAACATTGCATCAAAGGTAATATCAACTCTAA
TTGAAGATGAAATATTAACACACATGGTATTTACAGTACAAAAAGAACTAGCAGACAGAATGATTGCCAAAGAAGGAAAC
AAAAATTATTCATCATTTACAATATTAGTACAATCACATTTTAATGTAATTAAAATAATGGATATTGATAAAAAAAATTT
TTATCCAATCCCTAAAGTTAAATCAACAACCATAAAATTAACTCCTTGTAAAGGAAACATTAAAAATTTTCAAATATTCA
ATAAATTAATCAGAACAGTCTTTACAAGTCGTAGAAAAAAACTTAAAAATACAATTATCAACTTTATTAAAAATGAAAAC
ATTCTTAAAGAAGACTTTCTGAAAAACTTTTTAGATAAAAGACCTGAAGAAATCTCCGTTAAGGAATTCATTACAATTGC
AAATAAATTAACTACATATCATTAA

Upstream 100 bases:

>100_bases
GATACTATCTTACCTAATCTACAGGCTAGAACAAGAAAAATACTATAGAGAAAACATAAAAAACTCATGATAATAGCTAT
GTGATATTATTTTATTATCT

Downstream 100 bases:

>100_bases
GGCACTTGTTAATACAATATCATTTACAGAACAACCTCTTGAGAGATCACTAACAGGATTCTTTAATCCTTGCAAAAAAG
GACCGTATGCTCTAGCAAAA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MNINYNSINSIKNVLKNKNIAPRKIWGQNYLINENIREKIIDALDIKKNEKIWEIGPGLGAMTATLLNKANFLTAFEIDP
KYSEILNEKFGQLKNFKLIEGDFLKTYKQEKTSINKIVSNLPYNIASKVISTLIEDEILTHMVFTVQKELADRMIAKEGN
KNYSSFTILVQSHFNVIKIMDIDKKNFYPIPKVKSTTIKLTPCKGNIKNFQIFNKLIRTVFTSRRKKLKNTIINFIKNEN
ILKEDFLKNFLDKRPEEISVKEFITIANKLTTYH

Sequences:

>Translated_274_residues
MNINYNSINSIKNVLKNKNIAPRKIWGQNYLINENIREKIIDALDIKKNEKIWEIGPGLGAMTATLLNKANFLTAFEIDP
KYSEILNEKFGQLKNFKLIEGDFLKTYKQEKTSINKIVSNLPYNIASKVISTLIEDEILTHMVFTVQKELADRMIAKEGN
KNYSSFTILVQSHFNVIKIMDIDKKNFYPIPKVKSTTIKLTPCKGNIKNFQIFNKLIRTVFTSRRKKLKNTIINFIKNEN
ILKEDFLKNFLDKRPEEISVKEFITIANKLTTYH
>Mature_274_residues
MNINYNSINSIKNVLKNKNIAPRKIWGQNYLINENIREKIIDALDIKKNEKIWEIGPGLGAMTATLLNKANFLTAFEIDP
KYSEILNEKFGQLKNFKLIEGDFLKTYKQEKTSINKIVSNLPYNIASKVISTLIEDEILTHMVFTVQKELADRMIAKEGN
KNYSSFTILVQSHFNVIKIMDIDKKNFYPIPKVKSTTIKLTPCKGNIKNFQIFNKLIRTVFTSRRKKLKNTIINFIKNEN
ILKEDFLKNFLDKRPEEISVKEFITIANKLTTYH

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657198, Length=270, Percent_Identity=30, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI156415992, Length=295, Percent_Identity=25.4237288135593, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1786236, Length=265, Percent_Identity=27.1698113207547, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI25146882, Length=207, Percent_Identity=31.8840579710145, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324989, Length=212, Percent_Identity=29.7169811320755, Blast_Score=99, Evalue=6e-22,
Organism=Drosophila melanogaster, GI21358017, Length=228, Percent_Identity=29.8245614035088, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21357273, Length=295, Percent_Identity=23.0508474576271, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 31885; Mature: 31885

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNINYNSINSIKNVLKNKNIAPRKIWGQNYLINENIREKIIDALDIKKNEKIWEIGPGLG
CCCCHHHHHHHHHHHHCCCCCCCEECCCCCEECCHHHHHHHHHHCCCCCCCEEEECCCHH
AMTATLLNKANFLTAFEIDPKYSEILNEKFGQLKNFKLIEGDFLKTYKQEKTSINKIVSN
HHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHH
LPYNIASKVISTLIEDEILTHMVFTVQKELADRMIAKEGNKNYSSFTILVQSHFNVIKIM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEE
DIDKKNFYPIPKVKSTTIKLTPCKGNIKNFQIFNKLIRTVFTSRRKKLKNTIINFIKNEN
ECCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ILKEDFLKNFLDKRPEEISVKEFITIANKLTTYH
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNINYNSINSIKNVLKNKNIAPRKIWGQNYLINENIREKIIDALDIKKNEKIWEIGPGLG
CCCCHHHHHHHHHHHHCCCCCCCEECCCCCEECCHHHHHHHHHHCCCCCCCEEEECCCHH
AMTATLLNKANFLTAFEIDPKYSEILNEKFGQLKNFKLIEGDFLKTYKQEKTSINKIVSN
HHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHH
LPYNIASKVISTLIEDEILTHMVFTVQKELADRMIAKEGNKNYSSFTILVQSHFNVIKIM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEE
DIDKKNFYPIPKVKSTTIKLTPCKGNIKNFQIFNKLIRTVFTSRRKKLKNTIINFIKNEN
ECCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ILKEDFLKNFLDKRPEEISVKEFITIANKLTTYH
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA