Click here to switch to the map view.

The map label for this gene is metG

Identifier: 203284492

GI number: 203284492

Start: 620384

End: 622564

Strand: Reverse

Name: metG

Synonym: BDU_589

Alternate gene names: 203284492

Gene position: 622564-620384 (Counterclockwise)

Preceding gene: 203284494

Following gene: 203284491

Centisome position: 66.82

GC content: 27.65

Gene sequence:

>2181_bases
GTGAAAAAAAAGAACTTAATTACAGCAGCATTACCATATGTTAATAACATACCTCACCTTGGAAATTTAGTCCAAGTCTT
ATCTGCAGACGCTTTTGCAAGATATTCAAGAATGATGGGAATAGAAACACTTTATGTTTGTGGAACAGATGAATATGGCA
CAGCCACAGAAACTAAAGCCTTAATTGAAAAAACCACACCTGAAGAATTGTGCAACAAATATCATGCAATACACAAATCA
ATTTATGAATGGTTCAATATAAAATTTGATATTTTTGGACGCACAACTAATAAATATCATAAAGAAACAGTACAAGATTT
ATTCTTAAAGTTAGACAAAAATGGATATATTACAGAAAAAGAAAATGAACAATTTTTTTGTCAACAAGATAAAATGTTTT
TAGCAGATCGATACGTAACAGGAGAATGTCCAAATTGTGGAAATAACACAAAAGGAGATCAGTGTGAAAATTGCTCTAAT
TTACTAGTTACAAATGAACTACTAAACCCAAGATGCATAATTTGTAAAAATATTCCTATTATAAAAAAAACTAAGCATTT
ATACATTGATCTACCAAAGATAAAAAATGAATTGGAACATTGGATACAACAAATTGACCAAAACACAAATTGGAACACCA
ATGCCATTAAAATAACAAATGCATTTTTAAGAGATGGACTTAAGGAACGAACAATAACAAGAGATTTAAAATGGGGAATA
CCTGTCCCTAAAAAAGAATATGAAAATAAAGTATTTTATGTATGGTTTGATGCCCCAATTGGATATATATCAATTACAAA
AGAAATAATCAAAGATTGGGAATCTTGGTGGAAAAATAATGAAGATACAAATTTAATACAATTTATCGGAAAAGACAATA
TCTTATTTCATACTATTATGTTTCCTTCTATAGAACTTGGAAGTCAAGAAAATTGGACAATGTTAAATAAACTAGCTTCA
AGCGAATATCTAAATTATGAAAACTTAAAATTTTCAAAATCTGCAGGAACTGGAATATTTGGAAACGATGTTATTACCAC
TGGAATACCTTCTGATGTTTGGAGATTTTATATATATTACAACAGACCTGAAAAAGCTGATTTTCAATTTATGTGGGATG
ACTTTATGGAAAGAATCAATAGCGAACTCATAGGAAATTTTTCAAACCTTATTAATAGAGTATTAACATTTTATAAAAAA
TTCTTTGGAGATAAAATTGACAAAATAGAACTAAATGAAAATTTTTGGCAAATAGTAAATATTAAATATGAGAGAACAAT
AAATTTTTTCAAACAAATAGAATTAAAAGCAGCTTTAAAAGAAATTCTTGATATCTCAAGAATAGGTAACAAAATATTTC
AAGACAAAGAACCATGGAAAACAAAAAATAGTACGCCCCAAACAACAAAAGAATTACTCTTAAACCTGATATACTTAATA
AGAGATCTCTCCATATTAATCTCACCATTCATGCCACATACAAGCGATAGAATAAGAAGTTTTTTTGGTAAAAGTTATGA
AATATCCAATAAATTTTTAGGAACAAATTTAGGATTAACAACAATACAATCTACAGAAGTATTATTTACAAAAATAGAAA
AACAACTAATTGATAGTTTAAAATTAAAATATTCGGGGAGAACAAACATGCAAGATGAACAAAATAAAAATTCAATAAAT
TTATTTAGCGAACAAATATGCTTAAAAACAGTAAAAATCAAAACAATAGACAGAAATCCAGATGCAGAAAAATTATTCAT
CTTAAAACTTGACGATGGAACTCCTGAAGGAAAACAAATTGTAAGTAGCATTGCAGATCACTACACAGAAGAAGAACTAA
TTGGAAAACACATAATAATAGTTGACAATTTAAAACCTGCAAAATTTAGAGGAATCAGATCTGAGGGAATGTTAATTGCA
ACTGAAGATGAAAATAAAAATTTTAAAATAATAATTGTAGAAGATTTTAAAGATAATCCCATCCCTGGAGAAAGAGTGAT
ACTTGAAAGCGACACTGGAAAAGAATTAAAATCACCAACAAAAATTAACATAGATAAATTTCTCCAAGCCCAAATTGTGG
CAGAAAATGGAGAACTGAAAATAAATGGCATTAACTTAATATTAGAACATTCTAAAGAAAAAGTACTCTCAAAAGAAATT
CCCAATGGTAAGATATATTAA

Upstream 100 bases:

>100_bases
AAAAGCATAAATTAATAAAAAACTAAACAAAATGATATGTTAATATTAAAATTATGGATATTATGAATATAATGTATCAA
AAAAGGAAAAAATAAATCAA

Downstream 100 bases:

>100_bases
AACCCTAACTAAATAAAAACAATGGATACACAAAAAATAGAAATAGAAAACTTAAATGAAAATTATCTTCAAAGCAAGTT
ATGGGCAGTTGTAAAACAAA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 726; Mature: 726

Protein sequence:

>726_residues
MKKKNLITAALPYVNNIPHLGNLVQVLSADAFARYSRMMGIETLYVCGTDEYGTATETKALIEKTTPEELCNKYHAIHKS
IYEWFNIKFDIFGRTTNKYHKETVQDLFLKLDKNGYITEKENEQFFCQQDKMFLADRYVTGECPNCGNNTKGDQCENCSN
LLVTNELLNPRCIICKNIPIIKKTKHLYIDLPKIKNELEHWIQQIDQNTNWNTNAIKITNAFLRDGLKERTITRDLKWGI
PVPKKEYENKVFYVWFDAPIGYISITKEIIKDWESWWKNNEDTNLIQFIGKDNILFHTIMFPSIELGSQENWTMLNKLAS
SEYLNYENLKFSKSAGTGIFGNDVITTGIPSDVWRFYIYYNRPEKADFQFMWDDFMERINSELIGNFSNLINRVLTFYKK
FFGDKIDKIELNENFWQIVNIKYERTINFFKQIELKAALKEILDISRIGNKIFQDKEPWKTKNSTPQTTKELLLNLIYLI
RDLSILISPFMPHTSDRIRSFFGKSYEISNKFLGTNLGLTTIQSTEVLFTKIEKQLIDSLKLKYSGRTNMQDEQNKNSIN
LFSEQICLKTVKIKTIDRNPDAEKLFILKLDDGTPEGKQIVSSIADHYTEEELIGKHIIIVDNLKPAKFRGIRSEGMLIA
TEDENKNFKIIIVEDFKDNPIPGERVILESDTGKELKSPTKINIDKFLQAQIVAENGELKINGINLILEHSKEKVLSKEI
PNGKIY

Sequences:

>Translated_726_residues
MKKKNLITAALPYVNNIPHLGNLVQVLSADAFARYSRMMGIETLYVCGTDEYGTATETKALIEKTTPEELCNKYHAIHKS
IYEWFNIKFDIFGRTTNKYHKETVQDLFLKLDKNGYITEKENEQFFCQQDKMFLADRYVTGECPNCGNNTKGDQCENCSN
LLVTNELLNPRCIICKNIPIIKKTKHLYIDLPKIKNELEHWIQQIDQNTNWNTNAIKITNAFLRDGLKERTITRDLKWGI
PVPKKEYENKVFYVWFDAPIGYISITKEIIKDWESWWKNNEDTNLIQFIGKDNILFHTIMFPSIELGSQENWTMLNKLAS
SEYLNYENLKFSKSAGTGIFGNDVITTGIPSDVWRFYIYYNRPEKADFQFMWDDFMERINSELIGNFSNLINRVLTFYKK
FFGDKIDKIELNENFWQIVNIKYERTINFFKQIELKAALKEILDISRIGNKIFQDKEPWKTKNSTPQTTKELLLNLIYLI
RDLSILISPFMPHTSDRIRSFFGKSYEISNKFLGTNLGLTTIQSTEVLFTKIEKQLIDSLKLKYSGRTNMQDEQNKNSIN
LFSEQICLKTVKIKTIDRNPDAEKLFILKLDDGTPEGKQIVSSIADHYTEEELIGKHIIIVDNLKPAKFRGIRSEGMLIA
TEDENKNFKIIIVEDFKDNPIPGERVILESDTGKELKSPTKINIDKFLQAQIVAENGELKINGINLILEHSKEKVLSKEI
PNGKIY
>Mature_726_residues
MKKKNLITAALPYVNNIPHLGNLVQVLSADAFARYSRMMGIETLYVCGTDEYGTATETKALIEKTTPEELCNKYHAIHKS
IYEWFNIKFDIFGRTTNKYHKETVQDLFLKLDKNGYITEKENEQFFCQQDKMFLADRYVTGECPNCGNNTKGDQCENCSN
LLVTNELLNPRCIICKNIPIIKKTKHLYIDLPKIKNELEHWIQQIDQNTNWNTNAIKITNAFLRDGLKERTITRDLKWGI
PVPKKEYENKVFYVWFDAPIGYISITKEIIKDWESWWKNNEDTNLIQFIGKDNILFHTIMFPSIELGSQENWTMLNKLAS
SEYLNYENLKFSKSAGTGIFGNDVITTGIPSDVWRFYIYYNRPEKADFQFMWDDFMERINSELIGNFSNLINRVLTFYKK
FFGDKIDKIELNENFWQIVNIKYERTINFFKQIELKAALKEILDISRIGNKIFQDKEPWKTKNSTPQTTKELLLNLIYLI
RDLSILISPFMPHTSDRIRSFFGKSYEISNKFLGTNLGLTTIQSTEVLFTKIEKQLIDSLKLKYSGRTNMQDEQNKNSIN
LFSEQICLKTVKIKTIDRNPDAEKLFILKLDDGTPEGKQIVSSIADHYTEEELIGKHIIIVDNLKPAKFRGIRSEGMLIA
TEDENKNFKIIIVEDFKDNPIPGERVILESDTGKELKSPTKINIDKFLQAQIVAENGELKINGINLILEHSKEKVLSKEI
PNGKIY

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=557, Percent_Identity=46.1400359066427, Blast_Score=530, Evalue=1e-150,
Organism=Homo sapiens, GI24308436, Length=544, Percent_Identity=22.9779411764706, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI4507947, Length=117, Percent_Identity=29.9145299145299, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1788432, Length=675, Percent_Identity=26.2222222222222, Blast_Score=259, Evalue=4e-70,
Organism=Caenorhabditis elegans, GI17540794, Length=578, Percent_Identity=45.1557093425605, Blast_Score=524, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI212645096, Length=508, Percent_Identity=23.0314960629921, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71992781, Length=269, Percent_Identity=25.6505576208178, Blast_Score=87, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6321704, Length=554, Percent_Identity=44.2238267148014, Blast_Score=499, Evalue=1e-142,
Organism=Drosophila melanogaster, GI24655311, Length=573, Percent_Identity=43.804537521815, Blast_Score=497, Evalue=1e-140,
Organism=Drosophila melanogaster, GI21358483, Length=523, Percent_Identity=20.4588910133843, Blast_Score=108, Evalue=2e-23,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_BORDL (B5RME0)

Other databases:

- EMBL:   CP000976
- RefSeq:   YP_002222232.1
- EnsemblBacteria:   EBBORT00000001490
- GeneID:   6917815
- GenomeReviews:   CP000976_GR
- KEGG:   bdu:BDU_589
- GeneTree:   EBGT00050000007495
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- ProtClustDB:   PRK00133
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 84452; Mature: 84452

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 333-333

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKNLITAALPYVNNIPHLGNLVQVLSADAFARYSRMMGIETLYVCGTDEYGTATETKA
CCCCCEEEEECCHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHH
LIEKTTPEELCNKYHAIHKSIYEWFNIKFDIFGRTTNKYHKETVQDLFLKLDKNGYITEK
HHHCCCHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHEECCCCCEECC
ENEQFFCQQDKMFLADRYVTGECPNCGNNTKGDQCENCSNLLVTNELLNPRCIICKNIPI
CCCCEEECCCCEEEEECEECCCCCCCCCCCCCCCHHCCCCEEEECCCCCCEEEEECCCCC
IKKTKHLYIDLPKIKNELEHWIQQIDQNTNWNTNAIKITNAFLRDGLKERTITRDLKWGI
EECCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCHHHHHHHHHCCCCC
PVPKKEYENKVFYVWFDAPIGYISITKEIIKDWESWWKNNEDTNLIQFIGKDNILFHTIM
CCCCHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEEE
FPSIELGSQENWTMLNKLASSEYLNYENLKFSKSAGTGIFGNDVITTGIPSDVWRFYIYY
CCCEECCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCEEECCCCCCCEEEEEEE
NRPEKADFQFMWDDFMERINSELIGNFSNLINRVLTFYKKFFGDKIDKIELNENFWQIVN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEE
IKYERTINFFKQIELKAALKEILDISRIGNKIFQDKEPWKTKNSTPQTTKELLLNLIYLI
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
RDLSILISPFMPHTSDRIRSFFGKSYEISNKFLGTNLGLTTIQSTEVLFTKIEKQLIDSL
HHHHHHHCCCCCCCHHHHHHHHCCCEECCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHH
KLKYSGRTNMQDEQNKNSINLFSEQICLKTVKIKTIDRNPDAEKLFILKLDDGTPEGKQI
EEEECCCCCCCCCCCCCCHHHHHHHHHHEEEEEEEECCCCCCCEEEEEEECCCCCHHHHH
VSSIADHYTEEELIGKHIIIVDNLKPAKFRGIRSEGMLIATEDENKNFKIIIVEDFKDNP
HHHHHHHCCHHHHCCCEEEEEECCCCHHHCCCCCCCEEEEECCCCCCEEEEEEECCCCCC
IPGERVILESDTGKELKSPTKINIDKFLQAQIVAENGELKINGINLILEHSKEKVLSKEI
CCCCEEEEECCCCHHHCCCCEECHHHHHEEEEEECCCCEEEEEEEEEEECCHHHHHHHCC
PNGKIY
CCCCCC
>Mature Secondary Structure
MKKKNLITAALPYVNNIPHLGNLVQVLSADAFARYSRMMGIETLYVCGTDEYGTATETKA
CCCCCEEEEECCHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHH
LIEKTTPEELCNKYHAIHKSIYEWFNIKFDIFGRTTNKYHKETVQDLFLKLDKNGYITEK
HHHCCCHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHEECCCCCEECC
ENEQFFCQQDKMFLADRYVTGECPNCGNNTKGDQCENCSNLLVTNELLNPRCIICKNIPI
CCCCEEECCCCEEEEECEECCCCCCCCCCCCCCCHHCCCCEEEECCCCCCEEEEECCCCC
IKKTKHLYIDLPKIKNELEHWIQQIDQNTNWNTNAIKITNAFLRDGLKERTITRDLKWGI
EECCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCHHHHHHHHHCCCCC
PVPKKEYENKVFYVWFDAPIGYISITKEIIKDWESWWKNNEDTNLIQFIGKDNILFHTIM
CCCCHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEEE
FPSIELGSQENWTMLNKLASSEYLNYENLKFSKSAGTGIFGNDVITTGIPSDVWRFYIYY
CCCEECCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCEEECCCCCCCEEEEEEE
NRPEKADFQFMWDDFMERINSELIGNFSNLINRVLTFYKKFFGDKIDKIELNENFWQIVN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEE
IKYERTINFFKQIELKAALKEILDISRIGNKIFQDKEPWKTKNSTPQTTKELLLNLIYLI
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
RDLSILISPFMPHTSDRIRSFFGKSYEISNKFLGTNLGLTTIQSTEVLFTKIEKQLIDSL
HHHHHHHCCCCCCCHHHHHHHHCCCEECCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHH
KLKYSGRTNMQDEQNKNSINLFSEQICLKTVKIKTIDRNPDAEKLFILKLDDGTPEGKQI
EEEECCCCCCCCCCCCCCHHHHHHHHHHEEEEEEEECCCCCCCEEEEEEECCCCCHHHHH
VSSIADHYTEEELIGKHIIIVDNLKPAKFRGIRSEGMLIATEDENKNFKIIIVEDFKDNP
HHHHHHHCCHHHHCCCEEEEEECCCCHHHCCCCCCCEEEEECCCCCCEEEEEEECCCCCC
IPGERVILESDTGKELKSPTKINIDKFLQAQIVAENGELKINGINLILEHSKEKVLSKEI
CCCCEEEEECCCCHHHCCCCEECHHHHHEEEEEECCCCEEEEEEEEEEECCHHHHHHHCC
PNGKIY
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA