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The map label for this gene is fmt

Identifier: 203283990

GI number: 203283990

Start: 70039

End: 70968

Strand: Reverse

Name: fmt

Synonym: BDU_68

Alternate gene names: 203283990

Gene position: 70968-70039 (Counterclockwise)

Preceding gene: 203283991

Following gene: 203283989

Centisome position: 7.62

GC content: 29.89

Gene sequence:

>930_bases
TTGAGAATTTTTTTTGCAAGTTCTGATGTTATTGCTTTAGAGGTTTTAAAGAAAATATCAGATCAATATGATGTAGTTGG
AGTATTAACTGCACCTGATAAGCCTAGTGGTCGTGGTCTTTCTTTAAAAGTAAATGACATTAAACGTGAAGCTCTTAGTA
GAAAGATTACTGTTTTGCAACCAGTGGTACTTGATGCTGATGTAATAAACTTGGTAAAAAGCTTAGAACCTGAACTTATG
TTAGTTTTTTCTTATGGTAAGATTTTTAAACAAGAATTTTTGGATATTTTTCCAGTAGGTTGTATTAATATTCATCCTTC
TCTTTTGCCAAAATATAGAGGCCCTTCTCCTATTCAAACTGCTATTTTAAATGGTGATTCTATTAGTGGAATTACTGTTC
AAAAAATGACTTTGGAGATGGATAGTGGTAATATTTTGGCGCAAAGTCAGTTTGAAATAAAGAGCTTTAATACAAGTGTT
GATATTTTTGAATATGTTTCTTTAAATAGTTTTGATCTCGTAATAGAAGCTTTAAATAAACTGCTTAAAGGAGATATTGG
AATTGTTCAAGATAAAAATAATGCTACATATTGTTCTTTTTTGGGCAAAGAGCATAGGACTATTGATTTTAAGTTGAGTG
CCTTTGATATTAAGAATAAAATTAATGCGTATAATCCTTGGCCCCTTGCAAGAGCTAGACTTGATAATAATGAGATTATT
TTTCATAGAGCTGATTTTATAAGTACTAATGATTATGATGATCAAGTTATTGGAAAAATTATTGCTTTTGATCCTAGTAA
AGGTCTTTTGGTAAAGACAGGAGATGGAATCTTGGTAGTATTAGAGCTTCAAAGAATTGGGAAAAAAGTTTTAGATTGTG
TATCATTTTATCATGGAAATAGAGATTTAATAGGCAAGGTTTTTTCTTAA

Upstream 100 bases:

>100_bases
TGGATCATTTGAAAGGTGTACTTTTTATTGATTATTATGAGGATAAACTTAGGAATAAATTATTGAAATCTTATCTTAAG
GAAAGGAGGCTTGTTAAATT

Downstream 100 bases:

>100_bases
AATAAAGTGTAAGGAGATGATATAATATTGATGGATAATAAACCACCACATAATCAGTTATTTTTGGATAGTCAAAATCT
AAGTGATGGTAATTTGCATG

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MRIFFASSDVIALEVLKKISDQYDVVGVLTAPDKPSGRGLSLKVNDIKREALSRKITVLQPVVLDADVINLVKSLEPELM
LVFSYGKIFKQEFLDIFPVGCINIHPSLLPKYRGPSPIQTAILNGDSISGITVQKMTLEMDSGNILAQSQFEIKSFNTSV
DIFEYVSLNSFDLVIEALNKLLKGDIGIVQDKNNATYCSFLGKEHRTIDFKLSAFDIKNKINAYNPWPLARARLDNNEII
FHRADFISTNDYDDQVIGKIIAFDPSKGLLVKTGDGILVVLELQRIGKKVLDCVSFYHGNRDLIGKVFS

Sequences:

>Translated_309_residues
MRIFFASSDVIALEVLKKISDQYDVVGVLTAPDKPSGRGLSLKVNDIKREALSRKITVLQPVVLDADVINLVKSLEPELM
LVFSYGKIFKQEFLDIFPVGCINIHPSLLPKYRGPSPIQTAILNGDSISGITVQKMTLEMDSGNILAQSQFEIKSFNTSV
DIFEYVSLNSFDLVIEALNKLLKGDIGIVQDKNNATYCSFLGKEHRTIDFKLSAFDIKNKINAYNPWPLARARLDNNEII
FHRADFISTNDYDDQVIGKIIAFDPSKGLLVKTGDGILVVLELQRIGKKVLDCVSFYHGNRDLIGKVFS
>Mature_309_residues
MRIFFASSDVIALEVLKKISDQYDVVGVLTAPDKPSGRGLSLKVNDIKREALSRKITVLQPVVLDADVINLVKSLEPELM
LVFSYGKIFKQEFLDIFPVGCINIHPSLLPKYRGPSPIQTAILNGDSISGITVQKMTLEMDSGNILAQSQFEIKSFNTSV
DIFEYVSLNSFDLVIEALNKLLKGDIGIVQDKNNATYCSFLGKEHRTIDFKLSAFDIKNKINAYNPWPLARARLDNNEII
FHRADFISTNDYDDQVIGKIIAFDPSKGLLVKTGDGILVVLELQRIGKKVLDCVSFYHGNRDLIGKVFS

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI164663775, Length=319, Percent_Identity=25.705329153605, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1789683, Length=305, Percent_Identity=33.7704918032787, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1788589, Length=285, Percent_Identity=25.2631578947368, Blast_Score=102, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI133930964, Length=327, Percent_Identity=24.1590214067278, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI45550868, Length=226, Percent_Identity=27.8761061946903, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28571984, Length=149, Percent_Identity=30.8724832214765, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24585660, Length=322, Percent_Identity=23.9130434782609, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_BORDL (B5RKW3)

Other databases:

- EMBL:   CP000976
- RefSeq:   YP_002221730.1
- EnsemblBacteria:   EBBORT00000001616
- GeneID:   6918152
- GenomeReviews:   CP000976_GR
- KEGG:   bdu:BDU_68
- GeneTree:   EBGT00050000007279
- HOGENOM:   HBG571560
- OMA:   QIRACTP
- ProtClustDB:   CLSK507328
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 34475; Mature: 34475

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIFFASSDVIALEVLKKISDQYDVVGVLTAPDKPSGRGLSLKVNDIKREALSRKITVLQ
CEEEEECCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEEHHHHHHHHHCCEEEEC
PVVLDADVINLVKSLEPELMLVFSYGKIFKQEFLDIFPVGCINIHPSLLPKYRGPSPIQT
CEEECHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCEEECCHHHCCCCCCCCCHHE
AILNGDSISGITVQKMTLEMDSGNILAQSQFEIKSFNTSVDIFEYVSLNSFDLVIEALNK
EEECCCCCCCEEEEEEEEEECCCCEEEECEEEEEECCCCEEEEEEECCCHHHHHHHHHHH
LLKGDIGIVQDKNNATYCSFLGKEHRTIDFKLSAFDIKNKINAYNPWPLARARLDNNEII
HHCCCCEEEECCCCCEEEEECCCCCCEEEEEEEEEECCCCCCCCCCCCCCEEECCCCEEE
FHRADFISTNDYDDQVIGKIIAFDPSKGLLVKTGDGILVVLELQRIGKKVLDCVSFYHGN
EEECCCCCCCCCCHHHHEEEEEECCCCCEEEEECCCEEEEEEHHHHHHHHHHHHHHHCCC
RDLIGKVFS
HHHHHCCCC
>Mature Secondary Structure
MRIFFASSDVIALEVLKKISDQYDVVGVLTAPDKPSGRGLSLKVNDIKREALSRKITVLQ
CEEEEECCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEEHHHHHHHHHCCEEEEC
PVVLDADVINLVKSLEPELMLVFSYGKIFKQEFLDIFPVGCINIHPSLLPKYRGPSPIQT
CEEECHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCEEECCHHHCCCCCCCCCHHE
AILNGDSISGITVQKMTLEMDSGNILAQSQFEIKSFNTSVDIFEYVSLNSFDLVIEALNK
EEECCCCCCCEEEEEEEEEECCCCEEEECEEEEEECCCCEEEEEEECCCHHHHHHHHHHH
LLKGDIGIVQDKNNATYCSFLGKEHRTIDFKLSAFDIKNKINAYNPWPLARARLDNNEII
HHCCCCEEEECCCCCEEEEECCCCCCEEEEEEEEEECCCCCCCCCCCCCCEEECCCCEEE
FHRADFISTNDYDDQVIGKIIAFDPSKGLLVKTGDGILVVLELQRIGKKVLDCVSFYHGN
EEECCCCCCCCCCHHHHEEEEEECCCCCEEEEECCCEEEEEEHHHHHHHHHHHHHHHCCC
RDLIGKVFS
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA