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The map label for this gene is def [H]

Identifier: 203283991

GI number: 203283991

Start: 70965

End: 71462

Strand: Reverse

Name: def [H]

Synonym: BDU_69

Alternate gene names: 203283991

Gene position: 71462-70965 (Counterclockwise)

Preceding gene: 203283992

Following gene: 203283990

Centisome position: 7.67

GC content: 29.12

Gene sequence:

>498_bases
ATGGAAATAGTTTTTTATCCTGATGATTTGCTTCGAGTACAAACAAAGGATGTTGAAAATATTGATGATGAGTTGCGAAG
TATCATTTTTGAAATGATAGGTTTAATGGATAAAAGCAAGGGTGTTGGACTAGCTGCGCCTCAAGTAGGTCTTGATTTGT
CTATTTTTGTAGTTAGAAAAAATATGATGTCAAAACCTTTAGTGTTTATTAATCCTGTAATAACGTCAAAATCTGTTGAA
CTTAGTGTTTATAAAGAAGGTTGTTTAAGTATTCCTGGAGTTTATTATGATTTATCAAGGCCAAAATCAATTGTAATTGA
AGCTTATGATGAAAATGGCAAGTTTTTTAAGATTGAAGATTTAGATATTTTAGCCAGAATTATTCAGCATGAAATGGATC
ATTTGAAAGGTGTACTTTTTATTGATTATTATGAGGATAAACTTAGGAATAAATTATTGAAATCTTATCTTAAGGAAAGG
AGGCTTGTTAAATTTTGA

Upstream 100 bases:

>100_bases
TAAGAAAGGTGGTATTTATTTTTCGGAATCGGAAAAAGAAGAATGGAAATTTTTTGGACAAATAAAGTCATAATTTTAAA
AATGGATTTTTGGAGGATTT

Downstream 100 bases:

>100_bases
GAATTTTTTTTGCAAGTTCTGATGTTATTGCTTTAGAGGTTTTAAAGAAAATATCAGATCAATATGATGTAGTTGGAGTA
TTAACTGCACCTGATAAGCC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MEIVFYPDDLLRVQTKDVENIDDELRSIIFEMIGLMDKSKGVGLAAPQVGLDLSIFVVRKNMMSKPLVFINPVITSKSVE
LSVYKEGCLSIPGVYYDLSRPKSIVIEAYDENGKFFKIEDLDILARIIQHEMDHLKGVLFIDYYEDKLRNKLLKSYLKER
RLVKF

Sequences:

>Translated_165_residues
MEIVFYPDDLLRVQTKDVENIDDELRSIIFEMIGLMDKSKGVGLAAPQVGLDLSIFVVRKNMMSKPLVFINPVITSKSVE
LSVYKEGCLSIPGVYYDLSRPKSIVIEAYDENGKFFKIEDLDILARIIQHEMDHLKGVLFIDYYEDKLRNKLLKSYLKER
RLVKF
>Mature_165_residues
MEIVFYPDDLLRVQTKDVENIDDELRSIIFEMIGLMDKSKGVGLAAPQVGLDLSIFVVRKNMMSKPLVFINPVITSKSVE
LSVYKEGCLSIPGVYYDLSRPKSIVIEAYDENGKFFKIEDLDILARIIQHEMDHLKGVLFIDYYEDKLRNKLLKSYLKER
RLVKF

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=160, Percent_Identity=30.625, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1789682, Length=160, Percent_Identity=39.375, Blast_Score=99, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24645726, Length=156, Percent_Identity=30.7692307692308, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24645728, Length=155, Percent_Identity=32.9032258064516, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19126; Mature: 19126

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIVFYPDDLLRVQTKDVENIDDELRSIIFEMIGLMDKSKGVGLAAPQVGLDLSIFVVRK
CEEEECCHHHEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCEEEEEEEH
NMMSKPLVFINPVITSKSVELSVYKEGCLSIPGVYYDLSRPKSIVIEAYDENGKFFKIED
HHHCCCEEEEEEEECCCCEEEEEECCCCCCCCCEEEECCCCCEEEEEEECCCCCEEEEEC
LDILARIIQHEMDHLKGVLFIDYYEDKLRNKLLKSYLKERRLVKF
HHHHHHHHHHHHHHHCEEEEEEHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEIVFYPDDLLRVQTKDVENIDDELRSIIFEMIGLMDKSKGVGLAAPQVGLDLSIFVVRK
CEEEECCHHHEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCEEEEEEEH
NMMSKPLVFINPVITSKSVELSVYKEGCLSIPGVYYDLSRPKSIVIEAYDENGKFFKIED
HHHCCCEEEEEEEECCCCEEEEEECCCCCCCCCEEEECCCCCEEEEEEECCCCCEEEEEC
LDILARIIQHEMDHLKGVLFIDYYEDKLRNKLLKSYLKERRLVKF
HHHHHHHHHHHHHHHCEEEEEEHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]