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The map label for this gene is ruvA

Identifier: 203283953

GI number: 203283953

Start: 34158

End: 34748

Strand: Reverse

Name: ruvA

Synonym: BDU_29

Alternate gene names: 203283953

Gene position: 34748-34158 (Counterclockwise)

Preceding gene: 203283954

Following gene: 203283952

Centisome position: 3.73

GC content: 26.57

Gene sequence:

>591_bases
ATGATTAATAAAATTTATGGTAAGATTGTAGATAAAAAAGAATCTAGTATTATTATTTTAGCTTTTCCGTTTGAATTTGA
GATTTTAGTTAGTTCTTTTTGTAAAATGGAATTGAGGTTGTTAGAAGATGTTGAAATATTAACCTATTTTCATTTTAGAG
ATGATGATGTTAAGCTTTTTGGATTTTTAAATATATCAGAACGGGAAGTTTTTGAAGATTTAATTGGTGTTGATGGAATA
GGACCAAAAGCGGCTTTGAAAATATTATCTGGAATTAAATATGATGCATTTCGGCTTGCTATTGCAAAAGAAGATATTAA
TCTTATTTCTAAGGTTAAAGGTATTGGAAATAAAATAGCAGGGAAAATATTTTTAAAACTTAGAGGTAAACTTGTAAAGG
GTGATGAATCAAGTTCGTATATGTTGAAATTTAAAGAGCTTGAACAATCAATTGTGAATATGGGTTTTGATAGAAAATTG
GTTGTAGTTGCGTTTAGAGAAATTATGCTTAGTGATAAATTTTTAATTTTAAAAGAAGCCGAACAAGAACAATTTTTATT
TACAGAGACTTTAAAAAGACTTTCAGTTTAG

Upstream 100 bases:

>100_bases
GTTGATGATATTTGTTAGTATAGTGTTAAAGTTTTTTTATTTTGCTTTTATTTTTATAAATAAGATAAGCTTGAATTTTT
ATGAATTATAATTTAGTTCT

Downstream 100 bases:

>100_bases
ATTTTATTTTAGAGGTATATGATTAATTTTGAGTAAAAAGGGTGAAATTTAAGTGGATTTGGGCTTTTTAAATTCTGAAA
AAAATTTTCTTTATGACAAT

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MINKIYGKIVDKKESSIIILAFPFEFEILVSSFCKMELRLLEDVEILTYFHFRDDDVKLFGFLNISEREVFEDLIGVDGI
GPKAALKILSGIKYDAFRLAIAKEDINLISKVKGIGNKIAGKIFLKLRGKLVKGDESSSYMLKFKELEQSIVNMGFDRKL
VVVAFREIMLSDKFLILKEAEQEQFLFTETLKRLSV

Sequences:

>Translated_196_residues
MINKIYGKIVDKKESSIIILAFPFEFEILVSSFCKMELRLLEDVEILTYFHFRDDDVKLFGFLNISEREVFEDLIGVDGI
GPKAALKILSGIKYDAFRLAIAKEDINLISKVKGIGNKIAGKIFLKLRGKLVKGDESSSYMLKFKELEQSIVNMGFDRKL
VVVAFREIMLSDKFLILKEAEQEQFLFTETLKRLSV
>Mature_196_residues
MINKIYGKIVDKKESSIIILAFPFEFEILVSSFCKMELRLLEDVEILTYFHFRDDDVKLFGFLNISEREVFEDLIGVDGI
GPKAALKILSGIKYDAFRLAIAKEDINLISKVKGIGNKIAGKIFLKLRGKLVKGDESSSYMLKFKELEQSIVNMGFDRKL
VVVAFREIMLSDKFLILKEAEQEQFLFTETLKRLSV

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=138, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_BORDL (B5RLN1)

Other databases:

- EMBL:   CP000976
- RefSeq:   YP_002221693.1
- EnsemblBacteria:   EBBORT00000001090
- GeneID:   6918113
- GenomeReviews:   CP000976_GR
- KEGG:   bdu:BDU_29
- GeneTree:   EBGT00050000007395
- HOGENOM:   HBG635309
- OMA:   SEREVFE
- ProtClustDB:   PRK13901
- HAMAP:   MF_00031
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =3.6.4.12

Molecular weight: Translated: 22488; Mature: 22488

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINKIYGKIVDKKESSIIILAFPFEFEILVSSFCKMELRLLEDVEILTYFHFRDDDVKLF
CCCHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCEEEE
GFLNISEREVFEDLIGVDGIGPKAALKILSGIKYDAFRLAIAKEDINLISKVKGIGNKIA
EEECCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHEEEEEEHHHHHHHHHHHHCCHHHH
GKIFLKLRGKLVKGDESSSYMLKFKELEQSIVNMGFDRKLVVVAFREIMLSDKFLILKEA
HHEEEEECCEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECC
EQEQFLFTETLKRLSV
CCHHHHHHHHHHHHCC
>Mature Secondary Structure
MINKIYGKIVDKKESSIIILAFPFEFEILVSSFCKMELRLLEDVEILTYFHFRDDDVKLF
CCCHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCEEEE
GFLNISEREVFEDLIGVDGIGPKAALKILSGIKYDAFRLAIAKEDINLISKVKGIGNKIA
EEECCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHEEEEEEHHHHHHHHHHHHCCHHHH
GKIFLKLRGKLVKGDESSSYMLKFKELEQSIVNMGFDRKLVVVAFREIMLSDKFLILKEA
HHEEEEECCEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECC
EQEQFLFTETLKRLSV
CCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA