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The map label for this gene is spsK [H]

Identifier: 197120379

GI number: 197120379

Start: 4563782

End: 4565974

Strand: Direct

Name: spsK [H]

Synonym: Gbem_4020

Alternate gene names: 197120379

Gene position: 4563782-4565974 (Clockwise)

Preceding gene: 197120378

Following gene: 197120392

Centisome position: 98.89

GC content: 66.94

Gene sequence:

>2193_bases
ATGAGCAATGACAGGCATAAAGAGAGAGTCCCCGAGCTGTGGGGGGGGATGGAATGCACCCTGAACCGCGTGGGGAAGCA
CTTCCACAGCCAGCTCGCCCTGAACGGCCACCTGAGCCGCCCGAAGGATCTCGAACTCTTCGCCGGGCTCGGCATCCGGA
CGCTGCGCTACCCCGTGCTCTGGGAGATGGTAGCGCCGCAACGCCTCGATGCCCCGGACTGGCGCTGGACCGATGAGAGG
TTGAACCGGTTGCTGGAGCTCGGCCTTGAGCCCGTCGCCGGATTGCTGCACCACGGCAGCGGCCCCGCCTACACCAGCCT
CGTCGATCCAGATTTCCCCGCCCTATTGGCCCGCTACGCCCGCCTGGTCGCACGGCGCTACCCCTGGATCAGGTACTTCA
CCCCCGTCAACGAACCCCTCACCACCGCCCGCTTCAGCGGCCTCTACGGCCACTGGTACCCCCACGGCACCGACGACCGC
ACCTTCGTCCGCACCTTGCTGTCGCAGTGCCGCGGCATAGTGGAGGCGATGCGGGGCATCCGCGAAGTGACGCCAGAGGC
AAAGCTGGTGATGGCCGAGGACATGGGGAAGACCTTCTCCACGGATCTACTCCGCTACCAGGCCGACTACGAGAACCACC
GGCGCTGGCTGAGCCTCGACCTTTTGGCGGGGAAGGTGGATCGCTGCCATCCGCTACGCCTGTGGCTGGAGCAAAACGGG
ACCGACGATGCGGAGTTCGACTACTTCCTGCAGAGCGGCATGGCGCCGGACGTGGTCGGGCTCAACTACTACTTGACCAG
CGACCGCTTCCTCGACCACCGGCTGGACCGCTACCCCGAGTGGACCCACGGCGGGAACCACCAGCACCGCTACGCGGACG
TCGAGGCGGTGCGAGCCTGCCCGGAAGGGATACTGGGGCACGAAGAGGTGCTCCGCAGCGCCTGGCAGCGCTACGCAAAG
GAAGTGGCCCTCACCGAGGTGCACCTGGGTTCCACCCGCGAGGAGCAGTTACGCTGGTTCCACGAGGCGTGGCAAGCGGG
CGTGAAGCTTTGCCGCGAAGGGGTTCCGGTAAGAGCGGTGACCGCCTGGTCGCTTTTGGGCTCCTTCGACTGGTGCGGCC
TCTTCACCAGCATCACCGGCTCTTACGAGCCGGGAGTTTTCGACATCAGGGGGCCTTCCCCGCGCCCCACGCTCCTTGCC
GGGGGGCTGCGCCAGATCGTGCGCGAGGGGGAGTTGAGCCATCCAACGGTGAGAAGCCCAGGCTGGTGGCGCAGGCCGGA
CCGGTTTCTTCCCGGCCTGAACCCCGACCTCCCCTCCACGGGGGATGAGTGTGCAGAGGAGCGCGGGCGAATCCTCATCG
TCGGTAAGACCGGGACCTTGGGGCAGTCCTTCTCCCGGCTCTGCCGGCATCGCGGCATCCCCTGCCACCTCCTCTCCCGC
GGCGAAATCGATATCGCCTCTGCGGCCTCGGTCGCGACGGCCTTCGACCGCTACGAACCCTGGGCGGTGGTCAACGGCGC
GGGCTTCGTCCGGATCGACGACGCCGAGGGGGACGCCAGCACCTGCTTCAGGGAAAACACCGTGGGGCCCGCGGTGCTCG
CCGCCGAATGCGCCCGGCGCGGCGTGCCGCTGATGACCTTCTCCTCCGACATGGTCTTCAACGGCAGCAAGCGGACCGCT
TACCAGGAGCGGGATCCGGTATCGCCAATCAACATCTACGGGCAGAGCAAGGCCGAGGCGGAACGCCAGGTGCTTAAGCT
ATATCCAGGGGCACTGGTGTTGCGCACCAGCGCATTCTTCGGCCCCTGGGACCGGTACAACTTCGTCACCGTCACTTTGC
GCCGGCTCGCGGCCGGGGAAACTGTCGAAGCCGCCTCCGACCTGGTGGTCTCCCCCACTTACATCCCGGACCTGGTCCAT
GCCGGGCTCGACCTGCTCCTGGACGGCGAGAGCGGCATCTGGCACTTAAGCAACGCCGGCGCCGTGAGCTGGGCCGAGCT
CGCCCGCTGCTGCGCCAAAATGGCGGGATTGGATTCCTCCCGCGTCATCCCGCGCCCCGCGGCCTCCCTCGGCTACCTCG
CGCCACGCCCCCCCTTCTCGGTCCTGGGAAGCGAGCGCGGCGTGGTACTGCCGGAGCTGGAAAACGCATTGAGCCGATAC
TTCTTCGACACGGTCCCACCCGAAAACGAGTGA

Upstream 100 bases:

>100_bases
GCGGCGGAGGTAGCGTCGAACGTGTTGGCGCTCTACCGCGAGCAGGGGCAACAGGGGAAGCTATTGGTGCCGCAGTAGCC
GCGGCCGGCGGGAAAAGGCC

Downstream 100 bases:

>100_bases
TGCTGCACCCCTCCGCTTCCACAAAAAAAGGCCCCTTAACGGGGCCTTTTCAGTGACATCTCTAAAACCTGTTGCCGCGG
AGTTCACGGATAAAATCCCA

Product: bifunctional glycoside hydrolase/dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 730; Mature: 729

Protein sequence:

>730_residues
MSNDRHKERVPELWGGMECTLNRVGKHFHSQLALNGHLSRPKDLELFAGLGIRTLRYPVLWEMVAPQRLDAPDWRWTDER
LNRLLELGLEPVAGLLHHGSGPAYTSLVDPDFPALLARYARLVARRYPWIRYFTPVNEPLTTARFSGLYGHWYPHGTDDR
TFVRTLLSQCRGIVEAMRGIREVTPEAKLVMAEDMGKTFSTDLLRYQADYENHRRWLSLDLLAGKVDRCHPLRLWLEQNG
TDDAEFDYFLQSGMAPDVVGLNYYLTSDRFLDHRLDRYPEWTHGGNHQHRYADVEAVRACPEGILGHEEVLRSAWQRYAK
EVALTEVHLGSTREEQLRWFHEAWQAGVKLCREGVPVRAVTAWSLLGSFDWCGLFTSITGSYEPGVFDIRGPSPRPTLLA
GGLRQIVREGELSHPTVRSPGWWRRPDRFLPGLNPDLPSTGDECAEERGRILIVGKTGTLGQSFSRLCRHRGIPCHLLSR
GEIDIASAASVATAFDRYEPWAVVNGAGFVRIDDAEGDASTCFRENTVGPAVLAAECARRGVPLMTFSSDMVFNGSKRTA
YQERDPVSPINIYGQSKAEAERQVLKLYPGALVLRTSAFFGPWDRYNFVTVTLRRLAAGETVEAASDLVVSPTYIPDLVH
AGLDLLLDGESGIWHLSNAGAVSWAELARCCAKMAGLDSSRVIPRPAASLGYLAPRPPFSVLGSERGVVLPELENALSRY
FFDTVPPENE

Sequences:

>Translated_730_residues
MSNDRHKERVPELWGGMECTLNRVGKHFHSQLALNGHLSRPKDLELFAGLGIRTLRYPVLWEMVAPQRLDAPDWRWTDER
LNRLLELGLEPVAGLLHHGSGPAYTSLVDPDFPALLARYARLVARRYPWIRYFTPVNEPLTTARFSGLYGHWYPHGTDDR
TFVRTLLSQCRGIVEAMRGIREVTPEAKLVMAEDMGKTFSTDLLRYQADYENHRRWLSLDLLAGKVDRCHPLRLWLEQNG
TDDAEFDYFLQSGMAPDVVGLNYYLTSDRFLDHRLDRYPEWTHGGNHQHRYADVEAVRACPEGILGHEEVLRSAWQRYAK
EVALTEVHLGSTREEQLRWFHEAWQAGVKLCREGVPVRAVTAWSLLGSFDWCGLFTSITGSYEPGVFDIRGPSPRPTLLA
GGLRQIVREGELSHPTVRSPGWWRRPDRFLPGLNPDLPSTGDECAEERGRILIVGKTGTLGQSFSRLCRHRGIPCHLLSR
GEIDIASAASVATAFDRYEPWAVVNGAGFVRIDDAEGDASTCFRENTVGPAVLAAECARRGVPLMTFSSDMVFNGSKRTA
YQERDPVSPINIYGQSKAEAERQVLKLYPGALVLRTSAFFGPWDRYNFVTVTLRRLAAGETVEAASDLVVSPTYIPDLVH
AGLDLLLDGESGIWHLSNAGAVSWAELARCCAKMAGLDSSRVIPRPAASLGYLAPRPPFSVLGSERGVVLPELENALSRY
FFDTVPPENE
>Mature_729_residues
SNDRHKERVPELWGGMECTLNRVGKHFHSQLALNGHLSRPKDLELFAGLGIRTLRYPVLWEMVAPQRLDAPDWRWTDERL
NRLLELGLEPVAGLLHHGSGPAYTSLVDPDFPALLARYARLVARRYPWIRYFTPVNEPLTTARFSGLYGHWYPHGTDDRT
FVRTLLSQCRGIVEAMRGIREVTPEAKLVMAEDMGKTFSTDLLRYQADYENHRRWLSLDLLAGKVDRCHPLRLWLEQNGT
DDAEFDYFLQSGMAPDVVGLNYYLTSDRFLDHRLDRYPEWTHGGNHQHRYADVEAVRACPEGILGHEEVLRSAWQRYAKE
VALTEVHLGSTREEQLRWFHEAWQAGVKLCREGVPVRAVTAWSLLGSFDWCGLFTSITGSYEPGVFDIRGPSPRPTLLAG
GLRQIVREGELSHPTVRSPGWWRRPDRFLPGLNPDLPSTGDECAEERGRILIVGKTGTLGQSFSRLCRHRGIPCHLLSRG
EIDIASAASVATAFDRYEPWAVVNGAGFVRIDDAEGDASTCFRENTVGPAVLAAECARRGVPLMTFSSDMVFNGSKRTAY
QERDPVSPINIYGQSKAEAERQVLKLYPGALVLRTSAFFGPWDRYNFVTVTLRRLAAGETVEAASDLVVSPTYIPDLVHA
GLDLLLDGESGIWHLSNAGAVSWAELARCCAKMAGLDSSRVIPRPAASLGYLAPRPPFSVLGSERGVVLPELENALSRYF
FDTVPPENE

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=282, Percent_Identity=25.531914893617, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI11034825, Length=265, Percent_Identity=26.0377358490566, Blast_Score=70, Evalue=7e-12,
Organism=Escherichia coli, GI1788352, Length=288, Percent_Identity=25, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 81873; Mature: 81741

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDRHKERVPELWGGMECTLNRVGKHFHSQLALNGHLSRPKDLELFAGLGIRTLRYPVL
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHH
WEMVAPQRLDAPDWRWTDERLNRLLELGLEPVAGLLHHGSGPAYTSLVDPDFPALLARYA
HHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
RLVARRYPWIRYFTPVNEPLTTARFSGLYGHWYPHGTDDRTFVRTLLSQCRGIVEAMRGI
HHHHHHCCCEEEECCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
REVTPEAKLVMAEDMGKTFSTDLLRYQADYENHRRWLSLDLLAGKVDRCHPLRLWLEQNG
HHCCCCCEEEEHHHCCCHHHHHHHHHHHCHHHCCEEEEEHHHHCCCCCCCCEEEEEECCC
TDDAEFDYFLQSGMAPDVVGLNYYLTSDRFLDHRLDRYPEWTHGGNHQHRYADVEAVRAC
CCCHHHHHHHHCCCCCCEEEEEEEEECCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHC
PEGILGHEEVLRSAWQRYAKEVALTEVHLGSTREEQLRWFHEAWQAGVKLCREGVPVRAV
HHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHH
TAWSLLGSFDWCGLFTSITGSYEPGVFDIRGPSPRPTLLAGGLRQIVREGELSHPTVRSP
HHHHHHHCCHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
GWWRRPDRFLPGLNPDLPSTGDECAEERGRILIVGKTGTLGQSFSRLCRHRGIPCHLLSR
CCCCCCHHCCCCCCCCCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEECC
GEIDIASAASVATAFDRYEPWAVVNGAGFVRIDDAEGDASTCFRENTVGPAVLAAECARR
CCCCHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHC
GVPLMTFSSDMVFNGSKRTAYQERDPVSPINIYGQSKAEAERQVLKLYPGALVLRTSAFF
CCCEEEECCCEEECCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCEEEEEEHHHC
GPWDRYNFVTVTLRRLAAGETVEAASDLVVSPTYIPDLVHAGLDLLLDGESGIWHLSNAG
CCCCCCCHHHHHHHHHHCCCHHHHCCCCEECCHHHHHHHHCCCEEEEECCCCEEEECCCC
AVSWAELARCCAKMAGLDSSRVIPRPAASLGYLAPRPPFSVLGSERGVVLPELENALSRY
CCCHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCHHHHCCCCCEECHHHHHHHHHH
FFDTVPPENE
HHCCCCCCCC
>Mature Secondary Structure 
SNDRHKERVPELWGGMECTLNRVGKHFHSQLALNGHLSRPKDLELFAGLGIRTLRYPVL
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHH
WEMVAPQRLDAPDWRWTDERLNRLLELGLEPVAGLLHHGSGPAYTSLVDPDFPALLARYA
HHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
RLVARRYPWIRYFTPVNEPLTTARFSGLYGHWYPHGTDDRTFVRTLLSQCRGIVEAMRGI
HHHHHHCCCEEEECCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
REVTPEAKLVMAEDMGKTFSTDLLRYQADYENHRRWLSLDLLAGKVDRCHPLRLWLEQNG
HHCCCCCEEEEHHHCCCHHHHHHHHHHHCHHHCCEEEEEHHHHCCCCCCCCEEEEEECCC
TDDAEFDYFLQSGMAPDVVGLNYYLTSDRFLDHRLDRYPEWTHGGNHQHRYADVEAVRAC
CCCHHHHHHHHCCCCCCEEEEEEEEECCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHC
PEGILGHEEVLRSAWQRYAKEVALTEVHLGSTREEQLRWFHEAWQAGVKLCREGVPVRAV
HHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHH
TAWSLLGSFDWCGLFTSITGSYEPGVFDIRGPSPRPTLLAGGLRQIVREGELSHPTVRSP
HHHHHHHCCHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
GWWRRPDRFLPGLNPDLPSTGDECAEERGRILIVGKTGTLGQSFSRLCRHRGIPCHLLSR
CCCCCCHHCCCCCCCCCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEECC
GEIDIASAASVATAFDRYEPWAVVNGAGFVRIDDAEGDASTCFRENTVGPAVLAAECARR
CCCCHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHC
GVPLMTFSSDMVFNGSKRTAYQERDPVSPINIYGQSKAEAERQVLKLYPGALVLRTSAFF
CCCEEEECCCEEECCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCEEEEEEHHHC
GPWDRYNFVTVTLRRLAAGETVEAASDLVVSPTYIPDLVHAGLDLLLDGESGIWHLSNAG
CCCCCCCHHHHHHHHHHCCCHHHHCCCCEECCHHHHHHHHCCCEEEEECCCCEEEECCCC
AVSWAELARCCAKMAGLDSSRVIPRPAASLGYLAPRPPFSVLGSERGVVLPELENALSRY
CCCHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCHHHHCCCCCEECHHHHHHHHHH
FFDTVPPENE
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]