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The map label for this gene is ispD [H]

Identifier: 197120158

GI number: 197120158

Start: 4336254

End: 4336949

Strand: Direct

Name: ispD [H]

Synonym: Gbem_3797

Alternate gene names: 197120158

Gene position: 4336254-4336949 (Clockwise)

Preceding gene: 197120157

Following gene: 197120159

Centisome position: 93.96

GC content: 64.08

Gene sequence:

>696_bases
TTGAGCAGGATTTACGCGCTGATACCTGCCGCCGGAATGGGCAAAAGGATGGGTGCCGGCTCCAACAAGCAGTACCTGCT
GCTGGACGGCATGCCGATTCTCGCCCGCACCGTCGCAGCCTTCGAGGCGGCCCCCTTCATAGACGGGATCTACCTCGTTT
CGCCGGAGCAGGAGATCCCGTTTTGCCGCAGCGAGGTGGTCGACCATTACGGTTTTTCCAAGGTGCGCGCCATCGTCCCC
GGCGGGGCCGAGCGGCAGCACTCGGTCTGCAACGGGCTGGACGCCATGGCTGACGCTGCTGACGACGACCTGGTGCTGAT
CCACGACGGGGTGCGCCCTTTCGTTTCGCAAGAGATGCTGGAAGCCGCCGCTGACGCCGCCAGGGAACATGGCGCATCCG
TGGTCGCGGTCCCGGTGAAGGACACGGTGAAGGTGGTGAAAGGCGGCGTCATCTCCGAAACCCCGCCTCGCGAAGAGCTC
TGGTTGGCGCAAACGCCGCAGGCTTTCCGCTATGGCCTGATCCGCGATGCCCACGCGCGTGCAAAGGCGGAAGGGTATCT
GGGGACCGACGATGCGTCCCTCGTCGAGCATCAGGGGGGAGAGGTGCGCATCGTCACGGGCGACTACCGCAACATCAAGA
TCACGACGCCCGAGGACCTGGTCCTTGCCGAGGCGTTTTTGAAAGGAAAGGGATGA

Upstream 100 bases:

>100_bases
GTTGTAGATCGTATTCAGGCAGATTTATATGGTTTTTTCGTGCCCAATCTGTATAATTCGGCGGTTTCATGAACCGCCGA
CCGAACCTTCGAGGTAACTC

Downstream 100 bases:

>100_bases
GTCATATGCGTATCGGGCACGGTTACGACGTTCACAGGCTGGTGGAAGGGCGCAAGCTGATCCTCGGAGGGGTCGACGTC
CCTTACGCCAAGGGGCTCCT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MSRIYALIPAAGMGKRMGAGSNKQYLLLDGMPILARTVAAFEAAPFIDGIYLVSPEQEIPFCRSEVVDHYGFSKVRAIVP
GGAERQHSVCNGLDAMADAADDDLVLIHDGVRPFVSQEMLEAAADAAREHGASVVAVPVKDTVKVVKGGVISETPPREEL
WLAQTPQAFRYGLIRDAHARAKAEGYLGTDDASLVEHQGGEVRIVTGDYRNIKITTPEDLVLAEAFLKGKG

Sequences:

>Translated_231_residues
MSRIYALIPAAGMGKRMGAGSNKQYLLLDGMPILARTVAAFEAAPFIDGIYLVSPEQEIPFCRSEVVDHYGFSKVRAIVP
GGAERQHSVCNGLDAMADAADDDLVLIHDGVRPFVSQEMLEAAADAAREHGASVVAVPVKDTVKVVKGGVISETPPREEL
WLAQTPQAFRYGLIRDAHARAKAEGYLGTDDASLVEHQGGEVRIVTGDYRNIKITTPEDLVLAEAFLKGKG
>Mature_230_residues
SRIYALIPAAGMGKRMGAGSNKQYLLLDGMPILARTVAAFEAAPFIDGIYLVSPEQEIPFCRSEVVDHYGFSKVRAIVPG
GAERQHSVCNGLDAMADAADDDLVLIHDGVRPFVSQEMLEAAADAAREHGASVVAVPVKDTVKVVKGGVISETPPREELW
LAQTPQAFRYGLIRDAHARAKAEGYLGTDDASLVEHQGGEVRIVTGDYRNIKITTPEDLVLAEAFLKGKG

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=236, Percent_Identity=27.9661016949153, Blast_Score=91, Evalue=7e-19,
Organism=Homo sapiens, GI157671913, Length=136, Percent_Identity=31.6176470588235, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1789104, Length=229, Percent_Identity=37.5545851528384, Blast_Score=123, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 24801; Mature: 24669

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIYALIPAAGMGKRMGAGSNKQYLLLDGMPILARTVAAFEAAPFIDGIYLVSPEQEIP
CCCEEEEECCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCC
FCRSEVVDHYGFSKVRAIVPGGAERQHSVCNGLDAMADAADDDLVLIHDGVRPFVSQEML
HHHHHHHHHCCHHHEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHH
EAAADAAREHGASVVAVPVKDTVKVVKGGVISETPPREELWLAQTPQAFRYGLIRDAHAR
HHHHHHHHHCCCCEEEECCHHHHHHHCCCCCCCCCCHHHEEECCCCHHHHHHHHHHHHHH
AKAEGYLGTDDASLVEHQGGEVRIVTGDYRNIKITTPEDLVLAEAFLKGKG
HHCCCCCCCCCHHHHHCCCCEEEEEECCCCEEEEECCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRIYALIPAAGMGKRMGAGSNKQYLLLDGMPILARTVAAFEAAPFIDGIYLVSPEQEIP
CCEEEEECCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCC
FCRSEVVDHYGFSKVRAIVPGGAERQHSVCNGLDAMADAADDDLVLIHDGVRPFVSQEML
HHHHHHHHHCCHHHEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHH
EAAADAAREHGASVVAVPVKDTVKVVKGGVISETPPREELWLAQTPQAFRYGLIRDAHAR
HHHHHHHHHCCCCEEEECCHHHHHHHCCCCCCCCCCHHHEEECCCCHHHHHHHHHHHHHH
AKAEGYLGTDDASLVEHQGGEVRIVTGDYRNIKITTPEDLVLAEAFLKGKG
HHCCCCCCCCCHHHHHCCCCEEEEEECCCCEEEEECCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA