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The map label for this gene is gnfM [H]

Identifier: 197119178

GI number: 197119178

Start: 3234130

End: 3235566

Strand: Reverse

Name: gnfM [H]

Synonym: Gbem_2805

Alternate gene names: 197119178

Gene position: 3235566-3234130 (Counterclockwise)

Preceding gene: 197119179

Following gene: 197119177

Centisome position: 70.11

GC content: 61.93

Gene sequence:

>1437_bases
ATGTCCATCAACAACATATTGGTTGCCGACGACGAAGAGAGCATGCGCTGGGTCCTTTCCAAGGCGCTCAGGAAAAAAGG
CTTCAACGTGGAGCTCGCCCGCGACGGCAACGAGGCTCTCAGGCTGATTAAGGAGAACGTCTACGACCTGGCGCTTTTGG
ACATCAAGATGCCGGGGATGACGGGGCTCGAACTGCTAGACCGGGTGAAGGAGGAGCGCAGCGACCTCTTCGTCGTGATC
ATGACTGCCGAGGCGAGCATGAAAAACGCGGTCGAGGCGATGAAGCGCGGCGCCTACGATTACCTCACCAAGCCCTTCGA
CCTCGGGGTGATCGACGCGGTGGTCGAGAAGGTGAGCCGGGCGCGCGAGATGACCAGCCAGGTCTCGCTCCTCAAGGAAG
AGCTCAAGGACCGCTACCAGCTGGAGAAGACCATCATCGGCAACTCCCCGGCCATGAGGGAGGTCTACAAGACCATCGGC
AAGGTGGCTCCCAGCGACGTGACGGTGCTGGTGCAGGGCGAATCGGGGACGGGAAAGGAGCTCATCGCCCGCGCCATCCA
CTACAACTCAAAGCGTCTGGGCAAGCCCTTCATACCGCTCAACTGCGCCGCCATCCCCAAGGAGCTTCTGGAGAGCGAGC
TCTTCGGTTTCGAGAAAGGGGCCTTCACCGGCGCCACCGAGAGGAAACTCGGCAAGTTCGAGCAGGCCAACGGCGGCACC
ATCTTCCTGGACGAGATCGGAGACATGCCGCTCGACTTGCAGGCCAAGATCCTGAGGGTGCTGCAGGAGCGCGAGATCAC
CAGGACCGGCGGAAACCAGAGCATCCCGGTCGACGTCAGGGTGGTCGCTGCGACCAACCAGGACCTCGAAGAGAGCGTGC
ACAACAAGCAGTTCCGCGAGGACCTCTACTACCGGTTGAACGTGATACCGATCCATCTGGTGCCGCTCAGGGACAGGAAG
GAGGACGTGCCGCTTCTGGTGCAGTACTTCCTCACCAAGATCTGCGCCGAGCTCGAAGTGCCGCAAAAGCGCTGCTCCGC
CGACGCCATCAAGCTCCTGACCAACTACAGCTGGCCCGGCAACATCCGCGAGCTGGAAAACACCATCAAGAGGGGCGTCA
TCCTCTCGCCGGACCCACTCCTGGTCGCCTCCGATTTCCCGGGGCTGCGCAACCGCCAGAGCGAGGGCAAAGGGGAGGAG
CTTTCCCTGGAGGGGATCGTCGACCTGAAGCTGCGCGGCTGCTTCAGCAACATGGAGAAGATGGAGAGCGGCGACGTGCA
CGCCATGGTGCTGGAACAGGTGGAGCGGCCGCTGATCCGCTTCGTCCTGGAGAAGACCCGCGGCAACCAGGTCCGCGCCG
CCGACATCCTCGGCATCAACCGCAACACCCTGAGAAAGAAGATCACCGAGCTCGGCATCGAGCTGCGCAAGGAATAG

Upstream 100 bases:

>100_bases
TGACGCGAAACGGACGGTCAAGTCACAATGCAGATGCCCAATGGCGATGCCCGTAGCGAACGTTGAACATTGAACGTTGA
ACCGCTTTTTAGGAGATTTT

Downstream 100 bases:

>100_bases
GCTACCCCCACCCCATGACAAAGGAGTTACCCATGTCCCTGTTGGAGAGATTTTTTCTGGAGAGAGAGAACGCCCAGCTC
GTGGTGGTCGACGTGCAGGA

Product: nitrogen fixation master sigma-54-dependent transcriptional response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSINNILVADDEESMRWVLSKALRKKGFNVELARDGNEALRLIKENVYDLALLDIKMPGMTGLELLDRVKEERSDLFVVI
MTAEASMKNAVEAMKRGAYDYLTKPFDLGVIDAVVEKVSRAREMTSQVSLLKEELKDRYQLEKTIIGNSPAMREVYKTIG
KVAPSDVTVLVQGESGTGKELIARAIHYNSKRLGKPFIPLNCAAIPKELLESELFGFEKGAFTGATERKLGKFEQANGGT
IFLDEIGDMPLDLQAKILRVLQEREITRTGGNQSIPVDVRVVAATNQDLEESVHNKQFREDLYYRLNVIPIHLVPLRDRK
EDVPLLVQYFLTKICAELEVPQKRCSADAIKLLTNYSWPGNIRELENTIKRGVILSPDPLLVASDFPGLRNRQSEGKGEE
LSLEGIVDLKLRGCFSNMEKMESGDVHAMVLEQVERPLIRFVLEKTRGNQVRAADILGINRNTLRKKITELGIELRKE

Sequences:

>Translated_478_residues
MSINNILVADDEESMRWVLSKALRKKGFNVELARDGNEALRLIKENVYDLALLDIKMPGMTGLELLDRVKEERSDLFVVI
MTAEASMKNAVEAMKRGAYDYLTKPFDLGVIDAVVEKVSRAREMTSQVSLLKEELKDRYQLEKTIIGNSPAMREVYKTIG
KVAPSDVTVLVQGESGTGKELIARAIHYNSKRLGKPFIPLNCAAIPKELLESELFGFEKGAFTGATERKLGKFEQANGGT
IFLDEIGDMPLDLQAKILRVLQEREITRTGGNQSIPVDVRVVAATNQDLEESVHNKQFREDLYYRLNVIPIHLVPLRDRK
EDVPLLVQYFLTKICAELEVPQKRCSADAIKLLTNYSWPGNIRELENTIKRGVILSPDPLLVASDFPGLRNRQSEGKGEE
LSLEGIVDLKLRGCFSNMEKMESGDVHAMVLEQVERPLIRFVLEKTRGNQVRAADILGINRNTLRKKITELGIELRKE
>Mature_477_residues
SINNILVADDEESMRWVLSKALRKKGFNVELARDGNEALRLIKENVYDLALLDIKMPGMTGLELLDRVKEERSDLFVVIM
TAEASMKNAVEAMKRGAYDYLTKPFDLGVIDAVVEKVSRAREMTSQVSLLKEELKDRYQLEKTIIGNSPAMREVYKTIGK
VAPSDVTVLVQGESGTGKELIARAIHYNSKRLGKPFIPLNCAAIPKELLESELFGFEKGAFTGATERKLGKFEQANGGTI
FLDEIGDMPLDLQAKILRVLQEREITRTGGNQSIPVDVRVVAATNQDLEESVHNKQFREDLYYRLNVIPIHLVPLRDRKE
DVPLLVQYFLTKICAELEVPQKRCSADAIKLLTNYSWPGNIRELENTIKRGVILSPDPLLVASDFPGLRNRQSEGKGEEL
SLEGIVDLKLRGCFSNMEKMESGDVHAMVLEQVERPLIRFVLEKTRGNQVRAADILGINRNTLRKKITELGIELRKE

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=473, Percent_Identity=45.2431289640592, Blast_Score=403, Evalue=1e-113,
Organism=Escherichia coli, GI1790299, Length=481, Percent_Identity=43.6590436590437, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1790437, Length=465, Percent_Identity=43.6559139784946, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1788905, Length=466, Percent_Identity=38.6266094420601, Blast_Score=301, Evalue=6e-83,
Organism=Escherichia coli, GI87082117, Length=393, Percent_Identity=42.2391857506361, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI1789087, Length=333, Percent_Identity=47.1471471471471, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1789233, Length=346, Percent_Identity=43.3526011560694, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI87082152, Length=235, Percent_Identity=51.9148936170213, Blast_Score=238, Evalue=5e-64,
Organism=Escherichia coli, GI1786524, Length=355, Percent_Identity=39.7183098591549, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI87081872, Length=331, Percent_Identity=39.5770392749245, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI1787583, Length=260, Percent_Identity=42.3076923076923, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI87081858, Length=363, Percent_Identity=29.7520661157025, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1789828, Length=275, Percent_Identity=31.6363636363636, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788191, Length=116, Percent_Identity=37.0689655172414, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1787375, Length=174, Percent_Identity=28.735632183908, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1788713, Length=188, Percent_Identity=28.1914893617021, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1786599, Length=120, Percent_Identity=34.1666666666667, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI87082012, Length=131, Percent_Identity=29.7709923664122, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1789661, Length=92, Percent_Identity=36.9565217391304, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1786784, Length=119, Percent_Identity=30.2521008403361, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787487, Length=120, Percent_Identity=32.5, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789809, Length=116, Percent_Identity=29.3103448275862, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53850; Mature: 53719

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSINNILVADDEESMRWVLSKALRKKGFNVELARDGNEALRLIKENVYDLALLDIKMPGM
CCCCCEEEECCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHEEEEEEEECCCC
TGLELLDRVKEERSDLFVVIMTAEASMKNAVEAMKRGAYDYLTKPFDLGVIDAVVEKVSR
CHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHH
AREMTSQVSLLKEELKDRYQLEKTIIGNSPAMREVYKTIGKVAPSDVTVLVQGESGTGKE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHH
LIARAIHYNSKRLGKPFIPLNCAAIPKELLESELFGFEKGAFTGATERKLGKFEQANGGT
HHHHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCE
IFLDEIGDMPLDLQAKILRVLQEREITRTGGNQSIPVDVRVVAATNQDLEESVHNKQFRE
EEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHH
DLYYRLNVIPIHLVPLRDRKEDVPLLVQYFLTKICAELEVPQKRCSADAIKLLTNYSWPG
HHHEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
NIRELENTIKRGVILSPDPLLVASDFPGLRNRQSEGKGEELSLEGIVDLKLRGCFSNMEK
CHHHHHHHHHCCEEECCCCEEEECCCCCCCCCCCCCCCCEEEECCEEEHHHHHHHHHHHH
MESGDVHAMVLEQVERPLIRFVLEKTRGNQVRAADILGINRNTLRKKITELGIELRKE
HCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHEECCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SINNILVADDEESMRWVLSKALRKKGFNVELARDGNEALRLIKENVYDLALLDIKMPGM
CCCCEEEECCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHEEEEEEEECCCC
TGLELLDRVKEERSDLFVVIMTAEASMKNAVEAMKRGAYDYLTKPFDLGVIDAVVEKVSR
CHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHH
AREMTSQVSLLKEELKDRYQLEKTIIGNSPAMREVYKTIGKVAPSDVTVLVQGESGTGKE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHH
LIARAIHYNSKRLGKPFIPLNCAAIPKELLESELFGFEKGAFTGATERKLGKFEQANGGT
HHHHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCE
IFLDEIGDMPLDLQAKILRVLQEREITRTGGNQSIPVDVRVVAATNQDLEESVHNKQFRE
EEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHH
DLYYRLNVIPIHLVPLRDRKEDVPLLVQYFLTKICAELEVPQKRCSADAIKLLTNYSWPG
HHHEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
NIRELENTIKRGVILSPDPLLVASDFPGLRNRQSEGKGEELSLEGIVDLKLRGCFSNMEK
CHHHHHHHHHCCEEECCCCEEEECCCCCCCCCCCCCCCCEEEECCEEEHHHHHHHHHHHH
MESGDVHAMVLEQVERPLIRFVLEKTRGNQVRAADILGINRNTLRKKITELGIELRKE
HCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHEECCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7553451 [H]