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The map label for this gene is gnfL [H]

Identifier: 197119179

GI number: 197119179

Start: 3235826

End: 3236911

Strand: Reverse

Name: gnfL [H]

Synonym: Gbem_2806

Alternate gene names: 197119179

Gene position: 3236911-3235826 (Counterclockwise)

Preceding gene: 197119180

Following gene: 197119178

Centisome position: 70.14

GC content: 62.25

Gene sequence:

>1086_bases
ATGCCTGAGAAACTGGAGAGTTACTACGCCAACGTGATCGACAGCGTGGGTGACGGCGTGATCGTCCTCGACAACGCCGG
GGCCGTGACGCTGGTCAATCCCGCCGCCGAGGAGCTGGCCGGCGTTTCGCGCCGCCAGGCCATGGGCGTTCTCTTCAGCG
AGATCTTCAAAGGGGAAGGTCCTCTGAACGAGATGGTCGCCAAGACCGTGGAGACCGGGATGTCGGTCTCCGACCATGAG
AACATCGTGGTCAAGCGGGCGGGGAAGCTGATCCCAGTCGGCGCCAGCACTTCGCCGCTTTTGAGCGCAGGCGGGGAGCG
CATCGGCACCATTTTACTTTTGCGGGACCTGACCAACGTTCGCGAGTTGGAGTCGGCCGTGCGCCAGGCGGACCGCCTTT
CGGCCCTGGGGGGGCTAGCAGCGGGGCTCGCGCACGAGATCAAGAACCCTCTCGGCGGGATCAAGGGGGCGGCGCAGCTA
CTGGAACTGGAATTTCCCGACAACGAGGACCTGCGCGAGTACATCAGGGTGATGCTGAAGGAAGTACAGCGGGTCAACGT
CATCGTGGAGGAACTCCTGGCGCTCGCTTCGCCTGGGCGCCTGAAGCTCTCCAAGGTGAACCTGCACCGGGTCCTTTCCG
ATATCGTCTTATTGCAGAAAAACGCTAGCGAAGGGAAAGAGGTCTACCTCCAGCAGTACTTCGACCCGAGCATCCCTCCC
ATCCTGGGGGACGAAGCGCTTTTAACCCAGCTCTTCCTGAACCTGATCAAGAACGCGCTGGAGGCGGTGGAGGCCGGCGG
TGTGGTAAAGGTCACCAGCCGGGTGCTGTCGGACTATGCCATGACCCAGAAGGGGGAGCGGCGGGCGCGCATGGTGGCCA
TCGACATCGCCGACAACGGCCCGGGTATCGAGGCCGAGGTGCTGGAGAACATGTTCACCCCGTTTTTCACCACCAAGTCA
CAGGGTACCGGGTTGGGCCTGGCCATCTGCCAGAAGATCGTCTCAGAGCATCGGGGCATGATCAAGGTCGATTCCGACGC
CAAGCGCGGCACCGTCTTCACCGTGATGCTGCCGCTGGTGCAATAA

Upstream 100 bases:

>100_bases
TGGTATTCCAAGGGGCTGCCTGGGGCGGCGCAGTTTCGCGCGGCGGTGAACCGGATCGAAAGCGCTCCGGAGCTGATCCG
GGCGATGGAGGAGTTCTTCG

Downstream 100 bases:

>100_bases
ACAAGAAACCGTTCAACGTTCCAGGTTCAACGTTCGAGTGGCCTTGCCCGTTGACGGCGCAATTACCCTGACTTTGACCT
GCAGCGATAAAGACCGTTCA

Product: nitrogen fixation master sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MPEKLESYYANVIDSVGDGVIVLDNAGAVTLVNPAAEELAGVSRRQAMGVLFSEIFKGEGPLNEMVAKTVETGMSVSDHE
NIVVKRAGKLIPVGASTSPLLSAGGERIGTILLLRDLTNVRELESAVRQADRLSALGGLAAGLAHEIKNPLGGIKGAAQL
LELEFPDNEDLREYIRVMLKEVQRVNVIVEELLALASPGRLKLSKVNLHRVLSDIVLLQKNASEGKEVYLQQYFDPSIPP
ILGDEALLTQLFLNLIKNALEAVEAGGVVKVTSRVLSDYAMTQKGERRARMVAIDIADNGPGIEAEVLENMFTPFFTTKS
QGTGLGLAICQKIVSEHRGMIKVDSDAKRGTVFTVMLPLVQ

Sequences:

>Translated_361_residues
MPEKLESYYANVIDSVGDGVIVLDNAGAVTLVNPAAEELAGVSRRQAMGVLFSEIFKGEGPLNEMVAKTVETGMSVSDHE
NIVVKRAGKLIPVGASTSPLLSAGGERIGTILLLRDLTNVRELESAVRQADRLSALGGLAAGLAHEIKNPLGGIKGAAQL
LELEFPDNEDLREYIRVMLKEVQRVNVIVEELLALASPGRLKLSKVNLHRVLSDIVLLQKNASEGKEVYLQQYFDPSIPP
ILGDEALLTQLFLNLIKNALEAVEAGGVVKVTSRVLSDYAMTQKGERRARMVAIDIADNGPGIEAEVLENMFTPFFTTKS
QGTGLGLAICQKIVSEHRGMIKVDSDAKRGTVFTVMLPLVQ
>Mature_360_residues
PEKLESYYANVIDSVGDGVIVLDNAGAVTLVNPAAEELAGVSRRQAMGVLFSEIFKGEGPLNEMVAKTVETGMSVSDHEN
IVVKRAGKLIPVGASTSPLLSAGGERIGTILLLRDLTNVRELESAVRQADRLSALGGLAAGLAHEIKNPLGGIKGAAQLL
ELEFPDNEDLREYIRVMLKEVQRVNVIVEELLALASPGRLKLSKVNLHRVLSDIVLLQKNASEGKEVYLQQYFDPSIPPI
LGDEALLTQLFLNLIKNALEAVEAGGVVKVTSRVLSDYAMTQKGERRARMVAIDIADNGPGIEAEVLENMFTPFFTTKSQ
GTGLGLAICQKIVSEHRGMIKVDSDAKRGTVFTVMLPLVQ

Specific function: Member of the two-component regulatory system AtoS/AtoC; may activate AtoC by phosphorylation [H]

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=350, Percent_Identity=34.8571428571429, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI1790436, Length=245, Percent_Identity=37.9591836734694, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1790300, Length=354, Percent_Identity=31.9209039548023, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI48994928, Length=375, Percent_Identity=23.2, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI145693157, Length=258, Percent_Identity=29.0697674418605, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788393, Length=235, Percent_Identity=26.3829787234043, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786600, Length=259, Percent_Identity=25.0965250965251, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790346, Length=234, Percent_Identity=25.6410256410256, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1789149, Length=252, Percent_Identity=26.5873015873016, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790567, Length=370, Percent_Identity=27.5675675675676, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789808, Length=226, Percent_Identity=26.5486725663717, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1786783, Length=241, Percent_Identity=25.3112033195021, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1788381, Length=161, Percent_Identity=27.9503105590062, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081816, Length=237, Percent_Identity=27.0042194092827, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786912, Length=258, Percent_Identity=27.1317829457364, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790861, Length=225, Percent_Identity=24.8888888888889, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 38892; Mature: 38760

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEKLESYYANVIDSVGDGVIVLDNAGAVTLVNPAAEELAGVSRRQAMGVLFSEIFKGEG
CCHHHHHHHHHHHHHCCCCEEEEECCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLNEMVAKTVETGMSVSDHENIVVKRAGKLIPVGASTSPLLSAGGERIGTILLLRDLTNV
CHHHHHHHHHHHCCCCCCCCCCCEECCCCEEECCCCCCHHHHCCCHHHHHHHHHHHHHHH
RELESAVRQADRLSALGGLAAGLAHEIKNPLGGIKGAAQLLELEFPDNEDLREYIRVMLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEEECCCCHHHHHHHHHHHH
EVQRVNVIVEELLALASPGRLKLSKVNLHRVLSDIVLLQKNASEGKEVYLQQYFDPSIPP
HHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
ILGDEALLTQLFLNLIKNALEAVEAGGVVKVTSRVLSDYAMTQKGERRARMVAIDIADNG
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCC
PGIEAEVLENMFTPFFTTKSQGTGLGLAICQKIVSEHRGMIKVDSDAKRGTVFTVMLPLV
CCCHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCC
Q
C
>Mature Secondary Structure 
PEKLESYYANVIDSVGDGVIVLDNAGAVTLVNPAAEELAGVSRRQAMGVLFSEIFKGEG
CHHHHHHHHHHHHHCCCCEEEEECCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLNEMVAKTVETGMSVSDHENIVVKRAGKLIPVGASTSPLLSAGGERIGTILLLRDLTNV
CHHHHHHHHHHHCCCCCCCCCCCEECCCCEEECCCCCCHHHHCCCHHHHHHHHHHHHHHH
RELESAVRQADRLSALGGLAAGLAHEIKNPLGGIKGAAQLLELEFPDNEDLREYIRVMLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEEECCCCHHHHHHHHHHHH
EVQRVNVIVEELLALASPGRLKLSKVNLHRVLSDIVLLQKNASEGKEVYLQQYFDPSIPP
HHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
ILGDEALLTQLFLNLIKNALEAVEAGGVVKVTSRVLSDYAMTQKGERRARMVAIDIADNG
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCC
PGIEAEVLENMFTPFFTTKSQGTGLGLAICQKIVSEHRGMIKVDSDAKRGTVFTVMLPLV
CCCHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8346225; 9097040; 9278503 [H]