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The map label for this gene is dnaB-1 [H]

Identifier: 197119174

GI number: 197119174

Start: 3229869

End: 3231218

Strand: Direct

Name: dnaB-1 [H]

Synonym: Gbem_2801

Alternate gene names: 197119174

Gene position: 3229869-3231218 (Clockwise)

Preceding gene: 197119173

Following gene: 197119175

Centisome position: 69.98

GC content: 60.52

Gene sequence:

>1350_bases
ATGAACGCAACGGACATGAGAAAGCTGCCACCGCAGAGCATTGAGGCGGAGATGTCCATCCTGGGCGGGATCCTGGTGGA
TAACGAGGCGATCAACCGCGTCCTCGAAATCCTGGTGCCGGACGATATGTACCGGGAAAGCCACAGGAAGATCCTGCGCG
CCATGATCGATTTGAACGAGCGCGGGGAGCCTTGCGACCTGATCACCATGACCACCATCCTCAAGAAAAAAGGTGAGCTG
GAAGAGGTCGGCGGCGGGGCCTACCTGGCGACACTGGTCGACTTCGTCCCCATGGCCGCGAACATCTCCTACTACTGCAA
GATCGTGAAGGAGAAGTGGCTCACCAGGAAGCTCATCTCGGCGGCAACCGACATCGTGAGCAAGGGGTTCGAGGACAAGA
TCGATACCGAGGAACTCCTCGACGCAGCCCAGAAGGTGATCTTCGAGATCTCGGAGAACAAGATCCGCCCCGCCTACTAC
CAGGTGAGCGACATCCTCAAGGACACCATCAAGAACATCGAGCTCCTCTACGAGAAAAAGGAGCTGGTGACCGGCGTCCC
CACGGGCTACACGGACCTGGACAAGCTGACGGCCGGTTTTCACGCCGGCGACCTGGTCATCATCGCTGGACGCCCGGCAA
TGGGAAAGACCACCTTCGCCCTGAACGTGGCCCAGTACGCGGCCATCGACGCCGACAAAGCCTACCCCGCCGCCGTCTTT
TCCCTGGAGATGCCCAAGGAGCAGCTGGTGGAGAGGCTCCTTTGCTCCGTGGCGAAGGTCGACCTGAGCCGACTGCGAAG
CGGTCACCTGGTGGAGAACGACTGGCCCAAGCTGATCAAGGCCGGCGGCAAACTGCACGACGCGAAGATCTTCATCGACG
ACACCCCGTCGATCACCATCATGGAGCTCCGCTCCAAGGCGAGGCGCCTGAAGGCCGAGCACAACATCGGCCTCATCGTC
ATCGACTACCTGCAGCTCATGCGCGGCGGCGCCAACCCCGAAAGCCGCCAGCAGGAGATCTCGGAAATCTCCCGTTCGCT
CAAGGGTCTGGCCAAGGAGTTGAGCATTCCGGTCATCGCCCTGTCGCAGCTCAACCGCGGATTGGAGCAGCGCTCCGACA
AGCGCCCGATGATGAGCGACTTGAGGGAATCCGGAGCTATCGAGCAGGACGCCGACATCATCATGTTCGTCTACCGCGAA
GAGGTGTACGAGAAGGACAAAGAGGACCTCAAGGGTAAGGCTGAGGTCATCATCGGCAAGCACAGGAGCGGCCCGACCGG
AACGGTTCATCTGGCCTTCCGCGGCGAGTTCACCCGCTTCGAAAACCTGAGCGGCCGGGACGATTACTAA

Upstream 100 bases:

>100_bases
GCGCGACGGATGAAGGTACTATCCGACGGCTGTACCGTAACTCCCGACTGCCGTCGGGGCTTGCGGTACAGGTTAGGGTT
ACTGTCACTTAGAGGTCTGC

Downstream 100 bases:

>100_bases
GACAACGCAGCCTCCCAAAAGTCCCCCCTCCACCGGCGCGCAGGCGCGTCAAGGGAGGGGGAGAACTGTGAAAGCCTTGA
TGTGGGCGACGGCAGATCGC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MNATDMRKLPPQSIEAEMSILGGILVDNEAINRVLEILVPDDMYRESHRKILRAMIDLNERGEPCDLITMTTILKKKGEL
EEVGGGAYLATLVDFVPMAANISYYCKIVKEKWLTRKLISAATDIVSKGFEDKIDTEELLDAAQKVIFEISENKIRPAYY
QVSDILKDTIKNIELLYEKKELVTGVPTGYTDLDKLTAGFHAGDLVIIAGRPAMGKTTFALNVAQYAAIDADKAYPAAVF
SLEMPKEQLVERLLCSVAKVDLSRLRSGHLVENDWPKLIKAGGKLHDAKIFIDDTPSITIMELRSKARRLKAEHNIGLIV
IDYLQLMRGGANPESRQQEISEISRSLKGLAKELSIPVIALSQLNRGLEQRSDKRPMMSDLRESGAIEQDADIIMFVYRE
EVYEKDKEDLKGKAEVIIGKHRSGPTGTVHLAFRGEFTRFENLSGRDDY

Sequences:

>Translated_449_residues
MNATDMRKLPPQSIEAEMSILGGILVDNEAINRVLEILVPDDMYRESHRKILRAMIDLNERGEPCDLITMTTILKKKGEL
EEVGGGAYLATLVDFVPMAANISYYCKIVKEKWLTRKLISAATDIVSKGFEDKIDTEELLDAAQKVIFEISENKIRPAYY
QVSDILKDTIKNIELLYEKKELVTGVPTGYTDLDKLTAGFHAGDLVIIAGRPAMGKTTFALNVAQYAAIDADKAYPAAVF
SLEMPKEQLVERLLCSVAKVDLSRLRSGHLVENDWPKLIKAGGKLHDAKIFIDDTPSITIMELRSKARRLKAEHNIGLIV
IDYLQLMRGGANPESRQQEISEISRSLKGLAKELSIPVIALSQLNRGLEQRSDKRPMMSDLRESGAIEQDADIIMFVYRE
EVYEKDKEDLKGKAEVIIGKHRSGPTGTVHLAFRGEFTRFENLSGRDDY
>Mature_449_residues
MNATDMRKLPPQSIEAEMSILGGILVDNEAINRVLEILVPDDMYRESHRKILRAMIDLNERGEPCDLITMTTILKKKGEL
EEVGGGAYLATLVDFVPMAANISYYCKIVKEKWLTRKLISAATDIVSKGFEDKIDTEELLDAAQKVIFEISENKIRPAYY
QVSDILKDTIKNIELLYEKKELVTGVPTGYTDLDKLTAGFHAGDLVIIAGRPAMGKTTFALNVAQYAAIDADKAYPAAVF
SLEMPKEQLVERLLCSVAKVDLSRLRSGHLVENDWPKLIKAGGKLHDAKIFIDDTPSITIMELRSKARRLKAEHNIGLIV
IDYLQLMRGGANPESRQQEISEISRSLKGLAKELSIPVIALSQLNRGLEQRSDKRPMMSDLRESGAIEQDADIIMFVYRE
EVYEKDKEDLKGKAEVIIGKHRSGPTGTVHLAFRGEFTRFENLSGRDDY

Specific function: Participates In Initiation And Elongation During Chromosome Replication; It Exhibits DNA-Dependent ATPase Activity And Contains Distinct Active Sites For ATP Binding, DNA Binding, And Interaction With DNAc Protein, Primase, And Other Prepriming Proteins.

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=444, Percent_Identity=51.8018018018018, Blast_Score=441, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50317; Mature: 50317

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATDMRKLPPQSIEAEMSILGGILVDNEAINRVLEILVPDDMYRESHRKILRAMIDLNE
CCCCCCCCCCCHHHHHHHHHHHHHEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
RGEPCDLITMTTILKKKGELEEVGGGAYLATLVDFVPMAANISYYCKIVKEKWLTRKLIS
CCCCCHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
AATDIVSKGFEDKIDTEELLDAAQKVIFEISENKIRPAYYQVSDILKDTIKNIELLYEKK
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ELVTGVPTGYTDLDKLTAGFHAGDLVIIAGRPAMGKTTFALNVAQYAAIDADKAYPAAVF
HHHCCCCCCCCCHHHHHCCCCCCCEEEEECCCCCCCCEEEEEHHHHHHCCCCCCCCEEEE
SLEMPKEQLVERLLCSVAKVDLSRLRSGHLVENDWPKLIKAGGKLHDAKIFIDDTPSITI
EECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEEEEEEECCCCEEH
MELRSKARRLKAEHNIGLIVIDYLQLMRGGANPESRQQEISEISRSLKGLAKELSIPVIA
HHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHH
LSQLNRGLEQRSDKRPMMSDLRESGAIEQDADIIMFVYREEVYEKDKEDLKGKAEVIIGK
HHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEHHHHHHHHCCHHHCCCCEEEEEEC
HRSGPTGTVHLAFRGEFTRFENLSGRDDY
CCCCCCCEEEEEEECCCHHHCCCCCCCCC
>Mature Secondary Structure
MNATDMRKLPPQSIEAEMSILGGILVDNEAINRVLEILVPDDMYRESHRKILRAMIDLNE
CCCCCCCCCCCHHHHHHHHHHHHHEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
RGEPCDLITMTTILKKKGELEEVGGGAYLATLVDFVPMAANISYYCKIVKEKWLTRKLIS
CCCCCHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
AATDIVSKGFEDKIDTEELLDAAQKVIFEISENKIRPAYYQVSDILKDTIKNIELLYEKK
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ELVTGVPTGYTDLDKLTAGFHAGDLVIIAGRPAMGKTTFALNVAQYAAIDADKAYPAAVF
HHHCCCCCCCCCHHHHHCCCCCCCEEEEECCCCCCCCEEEEEHHHHHHCCCCCCCCEEEE
SLEMPKEQLVERLLCSVAKVDLSRLRSGHLVENDWPKLIKAGGKLHDAKIFIDDTPSITI
EECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEEEEEEECCCCEEH
MELRSKARRLKAEHNIGLIVIDYLQLMRGGANPESRQQEISEISRSLKGLAKELSIPVIA
HHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHH
LSQLNRGLEQRSDKRPMMSDLRESGAIEQDADIIMFVYREEVYEKDKEDLKGKAEVIIGK
HHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEHHHHHHHHCCHHHCCCCEEEEEEC
HRSGPTGTVHLAFRGEFTRFENLSGRDDY
CCCCCCCEEEEEEECCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1312531 [H]