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The map label for this gene is folA [H]

Identifier: 197119172

GI number: 197119172

Start: 3228120

End: 3229040

Strand: Direct

Name: folA [H]

Synonym: Gbem_2799

Alternate gene names: 197119172

Gene position: 3228120-3229040 (Clockwise)

Preceding gene: 197119171

Following gene: 197119173

Centisome position: 69.95

GC content: 63.19

Gene sequence:

>921_bases
ATGATCATCTCCACGATAGCGGCCTTGTCTGACAACAGGGTGATAGGCGCAGACGGAGGGCTCCCTTGGGATATCCCCTC
GGACCTCACTCGCTTTCGGTCCATCACCATGGGGCATACCGTCATCATGGGGAGAAAGACCTACGAGTCGATAGGGCATC
CGCTTTTGGGGCGCCGCAACATCGTGCTCACCAGGAAGGGGTCGCAGATAGAGGGGTGCGAGATCGCCCGCAACGTCCCG
GCTGCAATCGCAGCAGCTGAAGGCGAGGAGGAGGTCTTCATCTGCGGCGGCGGCGAAGTCTTTCAGGAGGCGCTGCCGCT
TTGCCAGCGGCTCTATCTCACCGTCGTCCACGGCAACTACCGGGGGGATGCCTTCTTGCCCGAACTTCCCGAGGGGTTCG
TGGAGCTGCACCGGGAGGAATTCCCCGACACCACCCCGCCTTCGAGCTTCGTGGTGCTGGAGAAGGTGGAGCGGGTCGAG
ACGGGGGCGGACGCCGAACAGTTGCGCCGCAAGGGACGGGAGGCGCTGAACCGCCAGCTCTACTTCCTGGCCCGGCGCTG
TTTCGAACAGGCGCAGGCACTGGAGGAAAACCCGGAAACCGCGTCAGATCTCGCCTACAGTCTGATCAAAAGCGGCGCTA
CAGGGCGCCAGGCACGGGACATGGCGGAAAGGGCGCTGCTGCAACAGCCGAAAAATCTCCGCATACTCCTCAATGCAGGG
AGGGTACTCATCCTCTCCGGCGAGAAGAGCAAAGGTCTCGACACGCTGCGCAGGGGTGTGCAGGCGGGAGGCGGCCCGGA
GTTTGTTGCCGAGCTCGCGCGCTGCGGCACCAGAAAGCTGCAGCCCATCAAAAGCCTGCCGAGGAGCCACCCGCTGAACC
GGTACCTGGGCCTATTGCTGCACCGGATGAACCTCAAGTAG

Upstream 100 bases:

>100_bases
ACCGGCGAGCGCTACGAAGGGGGCGATGGGGTGACGGTGGTGACCCTGCGCTGACGATGGACGGTAAGACCTTTATGCAC
TAACGACCTCGGAGGTCATG

Downstream 100 bases:

>100_bases
GGGCTCTCTCCCCCACCCTCCTCACCGCTTGACTTGATCCGGCGGCCGATGTACCTTCGACCGATGCGAAAAACCGAGGC
AGAGGCCATAGTGCTCCGCT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MIISTIAALSDNRVIGADGGLPWDIPSDLTRFRSITMGHTVIMGRKTYESIGHPLLGRRNIVLTRKGSQIEGCEIARNVP
AAIAAAEGEEEVFICGGGEVFQEALPLCQRLYLTVVHGNYRGDAFLPELPEGFVELHREEFPDTTPPSSFVVLEKVERVE
TGADAEQLRRKGREALNRQLYFLARRCFEQAQALEENPETASDLAYSLIKSGATGRQARDMAERALLQQPKNLRILLNAG
RVLILSGEKSKGLDTLRRGVQAGGGPEFVAELARCGTRKLQPIKSLPRSHPLNRYLGLLLHRMNLK

Sequences:

>Translated_306_residues
MIISTIAALSDNRVIGADGGLPWDIPSDLTRFRSITMGHTVIMGRKTYESIGHPLLGRRNIVLTRKGSQIEGCEIARNVP
AAIAAAEGEEEVFICGGGEVFQEALPLCQRLYLTVVHGNYRGDAFLPELPEGFVELHREEFPDTTPPSSFVVLEKVERVE
TGADAEQLRRKGREALNRQLYFLARRCFEQAQALEENPETASDLAYSLIKSGATGRQARDMAERALLQQPKNLRILLNAG
RVLILSGEKSKGLDTLRRGVQAGGGPEFVAELARCGTRKLQPIKSLPRSHPLNRYLGLLLHRMNLK
>Mature_306_residues
MIISTIAALSDNRVIGADGGLPWDIPSDLTRFRSITMGHTVIMGRKTYESIGHPLLGRRNIVLTRKGSQIEGCEIARNVP
AAIAAAEGEEEVFICGGGEVFQEALPLCQRLYLTVVHGNYRGDAFLPELPEGFVELHREEFPDTTPPSSFVVLEKVERVE
TGADAEQLRRKGREALNRQLYFLARRCFEQAQALEENPETASDLAYSLIKSGATGRQARDMAERALLQQPKNLRILLNAG
RVLILSGEKSKGLDTLRRGVQAGGGPEFVAELARCGTRKLQPIKSLPRSHPLNRYLGLLLHRMNLK

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=158, Percent_Identity=39.2405063291139, Blast_Score=116, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324810, Length=191, Percent_Identity=29.3193717277487, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI116008026, Length=160, Percent_Identity=35, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24647458, Length=160, Percent_Identity=35, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 33833; Mature: 33833

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIISTIAALSDNRVIGADGGLPWDIPSDLTRFRSITMGHTVIMGRKTYESIGHPLLGRRN
CCCHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHCCCCCCCCC
IVLTRKGSQIEGCEIARNVPAAIAAAEGEEEVFICGGGEVFQEALPLCQRLYLTVVHGNY
EEEEECCCCCCCHHHHHCCCHHHEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHEECCC
RGDAFLPELPEGFVELHREEFPDTTPPSSFVVLEKVERVETGADAEQLRRKGREALNRQL
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
YFLARRCFEQAQALEENPETASDLAYSLIKSGATGRQARDMAERALLQQPKNLRILLNAG
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEECC
RVLILSGEKSKGLDTLRRGVQAGGGPEFVAELARCGTRKLQPIKSLPRSHPLNRYLGLLL
EEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHH
HRMNLK
HHHCCC
>Mature Secondary Structure
MIISTIAALSDNRVIGADGGLPWDIPSDLTRFRSITMGHTVIMGRKTYESIGHPLLGRRN
CCCHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHCCCCCCCCC
IVLTRKGSQIEGCEIARNVPAAIAAAEGEEEVFICGGGEVFQEALPLCQRLYLTVVHGNY
EEEEECCCCCCCHHHHHCCCHHHEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHEECCC
RGDAFLPELPEGFVELHREEFPDTTPPSSFVVLEKVERVETGADAEQLRRKGREALNRQL
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
YFLARRCFEQAQALEENPETASDLAYSLIKSGATGRQARDMAERALLQQPKNLRILLNAG
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEECC
RVLILSGEKSKGLDTLRRGVQAGGGPEFVAELARCGTRKLQPIKSLPRSHPLNRYLGLLL
EEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHH
HRMNLK
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]