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The map label for this gene is ilvD

Identifier: 197119120

GI number: 197119120

Start: 3176528

End: 3178189

Strand: Reverse

Name: ilvD

Synonym: Gbem_2747

Alternate gene names: 197119120

Gene position: 3178189-3176528 (Counterclockwise)

Preceding gene: 197119121

Following gene: 197119119

Centisome position: 68.86

GC content: 66.25

Gene sequence:

>1662_bases
ATGCGTAGCGACACTATCACCCAAGGTTTGGAACGGACCCCGCACCGCGCGCTCTTGAAAGGGACCGGGCTTCCGCAAAG
CGAGATGGGGAAGCCGTTCATCGGCATCGCTACCAGCTTCACCGATCTGATTCCGGGGCACGTCGGCATGCGCGACCTGG
AGCGTTTCATCGAGAAGGGTGTCCACACCGGCGGCGGTTATTCCTTCTTCTTCGGGATACCCGGGGTCTGCGACGGCATC
TCCATGGGGCACAAGGGGATGCACTACTCCCTCCCCACCCGCGAGCTGATCGCGGACATGGTCGAGTCGGTCGCCGAGGC
GCATCGCCTGGACGGCCTCGTGCTCTTGACCAACTGCGACAAGATCACCCCGGGCATGCTCATGGCTGCCGCGAGGCTCG
ACATCCCCTGCATCGTAGTCACCGCCGGCCCCATGATGAGCGGCCGCGGCGACGCAGGTCGTAAGTATTCCTTCGTCACC
GACACCTTCGAGGCCATGGCGCGCTACAAGGCGGGGGTCATCGACGACGCGGAGCTCGCGCGCTGCGAGGAGAACGCCTG
CCCGGGCATGGGTTCCTGCCAGGGGCTTTTCACCGCCAACACCATGGCCATCCTCACCGAGACGCTCGGCATGAGCCTGC
CGCGCTGCGGCACCGCACTCGCCGTCTCCGCGCTCAAGCGCCGCATCGCCTTCGCCTCCGGCGAGCGCATCGTCGACCTG
GTGCGCCAGAACATCACCCCGCGCTCCATCCTGACCCGCGAGGCGTTCGAGAACGCCATCAGGGTTGACCTGGCGCTGGG
CGGCTCCTCCAACACGGTGCTGCACCTCTTGGCCATCGCCCACGAGGCAGGCGTCGAGCTCCCGCTGGAGACCTTCGACA
TCCTCGCCAAGGAGACCCCGCAGCTTGCCTCCATGAACCCGGCCGGCGAGCATTTCATGGAAGACCTGGACGTCGCAGGC
GGCGTCGCCGGGGTGCTGAAGCAGTTGGGCGACAAGATCCACGACTGCCCGACCCTCATGGGGCTCAGCACCAAGGAGAT
CGCGGCGAGCCTGAAGGGTGTCGACGAGGAAGTGATCCATCCGCTCTCGAACCCGGTCAAGAAGGAAGGTGGCATCGCCG
TTCTCTTCGGCAACATCTGCCCCAAGGGTGCTGTGGTCAAGCAGTCGGGCGTATCCGACAAGATGATGAAGTTCACCGGC
ACCGCGCGCTGCTTCGACTCCGAGGACAAGGCGATGGCTGCCATGATGGGTGGCGTGGTGAAGGGGGGAGACGTGGTCGT
CATCCGCTACGAAGGGCCCAAAGGGGGCCCGGGGATGCGCGAGATGCTCGCTCCCACCGCGGCGCTCATGGGGCTTGGGC
TTGGCGACTCCGTGGCGCTCATCACCGACGGCCGCTTCTCCGGCGGCACCCGCGGCCCCTGCATCGGCCACATCGCGCCC
GAAGCGGCGGCCGGCGGCCCGATCGGCCTCATCGAAGACGGCGACACCATTGAACTGGACATTCCGGCACGCTCGCTCAA
GGTCATGGTGAGCGACGAGGTGCTGGCAGAACGGCGCGCCCGCTGGGTCGCCCCCGAGCCGAAGATCAAGAAGGGTTGGC
TCGCCCGCTATGCGAAGGTGGTTACCTCGGCCCACACCGGCGCCATCACCACCGCTGAATAA

Upstream 100 bases:

>100_bases
CGTTCTCCTATAACCTCTCTTAAACACTCTGTTTTTGGCTATATCAACTGCCGGTTTGGCGATTCCGAACAGGGTCGCGC
CGGTTTAAGGGGAAATCTAT

Downstream 100 bases:

>100_bases
AACCTCGACCCCCTTTACCAAGGGGCAGGGGCAGGCACGCCTTTACCATGGCGGCGTCGGCCTCAGGGGACGGCTTCATT
ACTAGTGGTTCCAGGCTTAC

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MRSDTITQGLERTPHRALLKGTGLPQSEMGKPFIGIATSFTDLIPGHVGMRDLERFIEKGVHTGGGYSFFFGIPGVCDGI
SMGHKGMHYSLPTRELIADMVESVAEAHRLDGLVLLTNCDKITPGMLMAAARLDIPCIVVTAGPMMSGRGDAGRKYSFVT
DTFEAMARYKAGVIDDAELARCEENACPGMGSCQGLFTANTMAILTETLGMSLPRCGTALAVSALKRRIAFASGERIVDL
VRQNITPRSILTREAFENAIRVDLALGGSSNTVLHLLAIAHEAGVELPLETFDILAKETPQLASMNPAGEHFMEDLDVAG
GVAGVLKQLGDKIHDCPTLMGLSTKEIAASLKGVDEEVIHPLSNPVKKEGGIAVLFGNICPKGAVVKQSGVSDKMMKFTG
TARCFDSEDKAMAAMMGGVVKGGDVVVIRYEGPKGGPGMREMLAPTAALMGLGLGDSVALITDGRFSGGTRGPCIGHIAP
EAAAGGPIGLIEDGDTIELDIPARSLKVMVSDEVLAERRARWVAPEPKIKKGWLARYAKVVTSAHTGAITTAE

Sequences:

>Translated_553_residues
MRSDTITQGLERTPHRALLKGTGLPQSEMGKPFIGIATSFTDLIPGHVGMRDLERFIEKGVHTGGGYSFFFGIPGVCDGI
SMGHKGMHYSLPTRELIADMVESVAEAHRLDGLVLLTNCDKITPGMLMAAARLDIPCIVVTAGPMMSGRGDAGRKYSFVT
DTFEAMARYKAGVIDDAELARCEENACPGMGSCQGLFTANTMAILTETLGMSLPRCGTALAVSALKRRIAFASGERIVDL
VRQNITPRSILTREAFENAIRVDLALGGSSNTVLHLLAIAHEAGVELPLETFDILAKETPQLASMNPAGEHFMEDLDVAG
GVAGVLKQLGDKIHDCPTLMGLSTKEIAASLKGVDEEVIHPLSNPVKKEGGIAVLFGNICPKGAVVKQSGVSDKMMKFTG
TARCFDSEDKAMAAMMGGVVKGGDVVVIRYEGPKGGPGMREMLAPTAALMGLGLGDSVALITDGRFSGGTRGPCIGHIAP
EAAAGGPIGLIEDGDTIELDIPARSLKVMVSDEVLAERRARWVAPEPKIKKGWLARYAKVVTSAHTGAITTAE
>Mature_553_residues
MRSDTITQGLERTPHRALLKGTGLPQSEMGKPFIGIATSFTDLIPGHVGMRDLERFIEKGVHTGGGYSFFFGIPGVCDGI
SMGHKGMHYSLPTRELIADMVESVAEAHRLDGLVLLTNCDKITPGMLMAAARLDIPCIVVTAGPMMSGRGDAGRKYSFVT
DTFEAMARYKAGVIDDAELARCEENACPGMGSCQGLFTANTMAILTETLGMSLPRCGTALAVSALKRRIAFASGERIVDL
VRQNITPRSILTREAFENAIRVDLALGGSSNTVLHLLAIAHEAGVELPLETFDILAKETPQLASMNPAGEHFMEDLDVAG
GVAGVLKQLGDKIHDCPTLMGLSTKEIAASLKGVDEEVIHPLSNPVKKEGGIAVLFGNICPKGAVVKQSGVSDKMMKFTG
TARCFDSEDKAMAAMMGGVVKGGDVVVIRYEGPKGGPGMREMLAPTAALMGLGLGDSVALITDGRFSGGTRGPCIGHIAP
EAAAGGPIGLIEDGDTIELDIPARSLKVMVSDEVLAERRARWVAPEPKIKKGWLARYAKVVTSAHTGAITTAE

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=608, Percent_Identity=43.0921052631579, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI2367371, Length=479, Percent_Identity=33.8204592901879, Blast_Score=206, Evalue=4e-54,
Organism=Escherichia coli, GI1788157, Length=516, Percent_Identity=29.8449612403101, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1786464, Length=479, Percent_Identity=34.446764091858, Blast_Score=196, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6322476, Length=561, Percent_Identity=40.8199643493761, Blast_Score=402, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD_GEOBB (B5EHV2)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002139547.1
- ProteinModelPortal:   B5EHV2
- SMR:   B5EHV2
- GeneID:   6782745
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_2747
- HOGENOM:   HBG671001
- OMA:   AKETPQL
- ProtClustDB:   PRK00911
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 58340; Mature: 58340

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSDTITQGLERTPHRALLKGTGLPQSEMGKPFIGIATSFTDLIPGHVGMRDLERFIEKG
CCCCHHHHHHHCCCCHHHHHCCCCCHHHHCCCEEEHHHHHHHHCCCCCCHHHHHHHHHHC
VHTGGGYSFFFGIPGVCDGISMGHKGMHYSLPTRELIADMVESVAEAHRLDGLVLLTNCD
CCCCCCEEEEECCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCC
KITPGMLMAAARLDIPCIVVTAGPMMSGRGDAGRKYSFVTDTFEAMARYKAGVIDDAELA
CCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCHHHH
RCEENACPGMGSCQGLFTANTMAILTETLGMSLPRCGTALAVSALKRRIAFASGERIVDL
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
VRQNITPRSILTREAFENAIRVDLALGGSSNTVLHLLAIAHEAGVELPLETFDILAKETP
HHHCCCHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
QLASMNPAGEHFMEDLDVAGGVAGVLKQLGDKIHDCPTLMGLSTKEIAASLKGVDEEVIH
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHCCCCHHHHH
PLSNPVKKEGGIAVLFGNICPKGAVVKQSGVSDKMMKFTGTARCFDSEDKAMAAMMGGVV
HHCCCHHHCCCEEEEECCCCCCCCEEECCCCCHHHHHHCCCCEECCCCHHHHHHHHCCEE
KGGDVVVIRYEGPKGGPGMREMLAPTAALMGLGLGDSVALITDGRFSGGTRGPCIGHIAP
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEECCCCC
EAAAGGPIGLIEDGDTIELDIPARSLKVMVSDEVLAERRARWVAPEPKIKKGWLARYAKV
CCCCCCCEEEECCCCEEEEECCCCCEEEEECHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
VTSAHTGAITTAE
HHHCCCCCEEECC
>Mature Secondary Structure
MRSDTITQGLERTPHRALLKGTGLPQSEMGKPFIGIATSFTDLIPGHVGMRDLERFIEKG
CCCCHHHHHHHCCCCHHHHHCCCCCHHHHCCCEEEHHHHHHHHCCCCCCHHHHHHHHHHC
VHTGGGYSFFFGIPGVCDGISMGHKGMHYSLPTRELIADMVESVAEAHRLDGLVLLTNCD
CCCCCCEEEEECCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCC
KITPGMLMAAARLDIPCIVVTAGPMMSGRGDAGRKYSFVTDTFEAMARYKAGVIDDAELA
CCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCHHHH
RCEENACPGMGSCQGLFTANTMAILTETLGMSLPRCGTALAVSALKRRIAFASGERIVDL
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
VRQNITPRSILTREAFENAIRVDLALGGSSNTVLHLLAIAHEAGVELPLETFDILAKETP
HHHCCCHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
QLASMNPAGEHFMEDLDVAGGVAGVLKQLGDKIHDCPTLMGLSTKEIAASLKGVDEEVIH
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHCCCCHHHHH
PLSNPVKKEGGIAVLFGNICPKGAVVKQSGVSDKMMKFTGTARCFDSEDKAMAAMMGGVV
HHCCCHHHCCCEEEEECCCCCCCCEEECCCCCHHHHHHCCCCEECCCCHHHHHHHHCCEE
KGGDVVVIRYEGPKGGPGMREMLAPTAALMGLGLGDSVALITDGRFSGGTRGPCIGHIAP
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEECCCCC
EAAAGGPIGLIEDGDTIELDIPARSLKVMVSDEVLAERRARWVAPEPKIKKGWLARYAKV
CCCCCCCEEEECCCCEEEEECCCCCEEEEECHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
VTSAHTGAITTAE
HHHCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA