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The map label for this gene is ilvB [H]

Identifier: 197119119

GI number: 197119119

Start: 3174670

End: 3176370

Strand: Reverse

Name: ilvB [H]

Synonym: Gbem_2746

Alternate gene names: 197119119

Gene position: 3176370-3174670 (Counterclockwise)

Preceding gene: 197119120

Following gene: 197119118

Centisome position: 68.82

GC content: 64.2

Gene sequence:

>1701_bases
ATGAAAATGAACGGCGCACGAATTCTGTTGGAGTGCCTCAAACGGGAGGGGGTCGACACGATCTTCGGCTATCCCGGGGG
TACGGTCATCAACCTCTACGACGAGCTCTTCTCGTTCAAGGAGATCAGGCACATCCTGCCGCGTCACGAGCAGGCAGGGG
TCCACGCAGCGGACGGTTACGCACGCGCGACCGGGAAGGTAGGGGTCGCCATAGCCACCTCCGGTCCGGGCGCCACCAAC
ACCATCACCGGCATCGCCACCGCCTACATGGACTCCATCCCGATGGTGATCGTCACCGGGCAGGTTCCCACCGCGCTGAT
CGGCAACGACGCCTTCCAGGAAGCCGACATCATCGGCATCACCCGTCCCTGCACCAAGCACAACTTCCTGGTGAAGGATG
TCAAAGACCTCCCCACCATCATGAAGAAGGCCTTCTACATCGCCCGCACCGGCCGCCCCGGCCCGGTCCTCGTGGACCTG
CCGAAGGACGTGCAGATGGCGCAGGCCGAGTTCCACTACCCGGAGACGGTCGAGATCCGCGGCTACAAGCCGAACCTCGA
AGGGCACCCGAAGCAGGTGGAGAAGGCGGTAACCATGATCACCCAGGCCAAGCGCCCGGTGCTCTACGTGGGGGGCGGCG
TCATCCTCGGGAACGCCGCGGCGGAGCTCACCGCCTTTGCCAAGCGCCTCGCCATACCGGTCACCACCACCCTCATGGGG
CTCGGCGCCTTTCCGGAGAACGATACGCTGTCGCTGGGGCTTCTCGGCATGCACGGCACCTACTACGCCAACATGGCCGT
GTCGAACTGCGACGTCCTGATAGCCATCGGCGCCCGTTTCGACGACCGCGTCACCGGCAAGATCGCCTCCTTCGCGCCGC
ACGCGAAGATCGTGCACGTCGACGTCGACCCCACCTCCATCAAGAAGAACGTCCGCGTCGATCTCCCCATCGTGGGGGAT
GTGCGCGACGTCCTCGCCAAGATGCTGAAGGCGGCCGAGCTGGTCTGCCCCGACGCCAAGGTATGCCAGGACGCCATCGC
CCCCTGGACCGCCGAGATCGAGGGGTGGAAGGCGAAGCACCCGATGTCCTACAAGCAGTCCACCACCACCATCAAGCCGC
AGTACGTGATCCAGAGGCTGCGCGCGCTTTCCGACCCCGACGCCATCGTCGCCACCGACGTGGGGCAGCACCAGATGTGG
ACCGCGCAGTTCTTCGGTTTCACCACCCCCAGGACCCTTCTTTCCTCCGGCGGCCTCGGCACCATGGGTTACGGCCTTCC
CGCGGCCATGGGCGCGCAGGCTGCCTTTCCCGAGCGCCAGGTGATGGTGGTCTGCGGCGACGGCGGCTTCCAGATGAATC
TGCAGGAGCTAGCCACCATAGTGCAGAACCGGCTCAACGTGAAGATCTGCATCCTGAACAACAATTTTCTGGGCATGGTG
CGCCAGTGGCAGGAGCTCTTCTTCGACAAGCGCTACTCCTCCACCTGCATGGAACTCCCCATCGACTTCATAAAGCTCGC
CGAGGCCTTCGGCGCCACCGGTCTCCAGGCCACCAAGGTCGACGAGGTCGACGAGGTGATCAAGAAAGGGTTCGCCACCA
ACGGCCCGGTCCTGATGGAGTTCAAGGTCGCCCGCGAGGAGAAGGTGCTCCCGATGGTTCCCGCCGGAGCGTCCCTCACC
GAGATGGTGTTGGCGTCGTAA

Upstream 100 bases:

>100_bases
CGGCCTCAGGGGACGGCTTCATTACTAGTGGTTCCAGGCTTACCAAGCCCGGGACCGATCAATAGCAGGCAGGCAAACTG
CCCGCGGCATGGGGGAAAAC

Downstream 100 bases:

>100_bases
CCCCAATTGACGGCGTAACCCAATTGACGGCGTAACCCAATTGACGGCGTAACCCAATTGACAATTGACGATTGACAATT
GACAACGAAAAGTCATCAGT

Product: acetolactate synthase, large subunit, biosynthetic type

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MKMNGARILLECLKREGVDTIFGYPGGTVINLYDELFSFKEIRHILPRHEQAGVHAADGYARATGKVGVAIATSGPGATN
TITGIATAYMDSIPMVIVTGQVPTALIGNDAFQEADIIGITRPCTKHNFLVKDVKDLPTIMKKAFYIARTGRPGPVLVDL
PKDVQMAQAEFHYPETVEIRGYKPNLEGHPKQVEKAVTMITQAKRPVLYVGGGVILGNAAAELTAFAKRLAIPVTTTLMG
LGAFPENDTLSLGLLGMHGTYYANMAVSNCDVLIAIGARFDDRVTGKIASFAPHAKIVHVDVDPTSIKKNVRVDLPIVGD
VRDVLAKMLKAAELVCPDAKVCQDAIAPWTAEIEGWKAKHPMSYKQSTTTIKPQYVIQRLRALSDPDAIVATDVGQHQMW
TAQFFGFTTPRTLLSSGGLGTMGYGLPAAMGAQAAFPERQVMVVCGDGGFQMNLQELATIVQNRLNVKICILNNNFLGMV
RQWQELFFDKRYSSTCMELPIDFIKLAEAFGATGLQATKVDEVDEVIKKGFATNGPVLMEFKVAREEKVLPMVPAGASLT
EMVLAS

Sequences:

>Translated_566_residues
MKMNGARILLECLKREGVDTIFGYPGGTVINLYDELFSFKEIRHILPRHEQAGVHAADGYARATGKVGVAIATSGPGATN
TITGIATAYMDSIPMVIVTGQVPTALIGNDAFQEADIIGITRPCTKHNFLVKDVKDLPTIMKKAFYIARTGRPGPVLVDL
PKDVQMAQAEFHYPETVEIRGYKPNLEGHPKQVEKAVTMITQAKRPVLYVGGGVILGNAAAELTAFAKRLAIPVTTTLMG
LGAFPENDTLSLGLLGMHGTYYANMAVSNCDVLIAIGARFDDRVTGKIASFAPHAKIVHVDVDPTSIKKNVRVDLPIVGD
VRDVLAKMLKAAELVCPDAKVCQDAIAPWTAEIEGWKAKHPMSYKQSTTTIKPQYVIQRLRALSDPDAIVATDVGQHQMW
TAQFFGFTTPRTLLSSGGLGTMGYGLPAAMGAQAAFPERQVMVVCGDGGFQMNLQELATIVQNRLNVKICILNNNFLGMV
RQWQELFFDKRYSSTCMELPIDFIKLAEAFGATGLQATKVDEVDEVIKKGFATNGPVLMEFKVAREEKVLPMVPAGASLT
EMVLAS
>Mature_566_residues
MKMNGARILLECLKREGVDTIFGYPGGTVINLYDELFSFKEIRHILPRHEQAGVHAADGYARATGKVGVAIATSGPGATN
TITGIATAYMDSIPMVIVTGQVPTALIGNDAFQEADIIGITRPCTKHNFLVKDVKDLPTIMKKAFYIARTGRPGPVLVDL
PKDVQMAQAEFHYPETVEIRGYKPNLEGHPKQVEKAVTMITQAKRPVLYVGGGVILGNAAAELTAFAKRLAIPVTTTLMG
LGAFPENDTLSLGLLGMHGTYYANMAVSNCDVLIAIGARFDDRVTGKIASFAPHAKIVHVDVDPTSIKKNVRVDLPIVGD
VRDVLAKMLKAAELVCPDAKVCQDAIAPWTAEIEGWKAKHPMSYKQSTTTIKPQYVIQRLRALSDPDAIVATDVGQHQMW
TAQFFGFTTPRTLLSSGGLGTMGYGLPAAMGAQAAFPERQVMVVCGDGGFQMNLQELATIVQNRLNVKICILNNNFLGMV
RQWQELFFDKRYSSTCMELPIDFIKLAEAFGATGLQATKVDEVDEVIKKGFATNGPVLMEFKVAREEKVLPMVPAGASLT
EMVLAS

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=568, Percent_Identity=26.056338028169, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI93004078, Length=562, Percent_Identity=24.0213523131673, Blast_Score=146, Evalue=6e-35,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=48.7676056338028, Blast_Score=573, Evalue=1e-164,
Organism=Escherichia coli, GI1790104, Length=562, Percent_Identity=43.0604982206406, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1786717, Length=561, Percent_Identity=34.5811051693405, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI1787096, Length=555, Percent_Identity=30.2702702702703, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1788716, Length=550, Percent_Identity=27.2727272727273, Blast_Score=162, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17531299, Length=592, Percent_Identity=26.5202702702703, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17531301, Length=581, Percent_Identity=26.8502581755594, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17542570, Length=584, Percent_Identity=26.3698630136986, Blast_Score=145, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6323755, Length=583, Percent_Identity=46.8267581475129, Blast_Score=504, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6320123, Length=569, Percent_Identity=23.1985940246046, Blast_Score=117, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6320816, Length=482, Percent_Identity=25.103734439834, Blast_Score=110, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=485, Percent_Identity=25.5670103092783, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321524, Length=497, Percent_Identity=21.7303822937626, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323073, Length=574, Percent_Identity=22.4738675958188, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19922626, Length=557, Percent_Identity=26.032315978456, Blast_Score=161, Evalue=1e-39,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61329; Mature: 61329

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMNGARILLECLKREGVDTIFGYPGGTVINLYDELFSFKEIRHILPRHEQAGVHAADGY
CCCCHHHHHHHHHHHCCCCEEECCCCCCEEHHHHHHHHHHHHHHHCCCCHHCCCCCCCCC
ARATGKVGVAIATSGPGATNTITGIATAYMDSIPMVIVTGQVPTALIGNDAFQEADIIGI
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCCCCCEEEE
TRPCTKHNFLVKDVKDLPTIMKKAFYIARTGRPGPVLVDLPKDVQMAQAEFHYPETVEIR
CCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCCCHHHHHHHCCCCCEEEEE
GYKPNLEGHPKQVEKAVTMITQAKRPVLYVGGGVILGNAAAELTAFAKRLAIPVTTTLMG
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEECCHHHHHHHHHHHHCCCHHHHHHH
LGAFPENDTLSLGLLGMHGTYYANMAVSNCDVLIAIGARFDDRVTGKIASFAPHAKIVHV
CCCCCCCCCEEEEEEECCCEEEEEEEECCCCEEEEECCCCCCCHHCHHHHCCCCCEEEEE
DVDPTSIKKNVRVDLPIVGDVRDVLAKMLKAAELVCPDAKVCQDAIAPWTAEIEGWKAKH
ECCHHHHCCCCEEECCEECCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEECCCCCCCCC
PMSYKQSTTTIKPQYVIQRLRALSDPDAIVATDVGQHQMWTAQFFGFTTPRTLLSSGGLG
CCCCCCCCCEECHHHHHHHHHHCCCCCCEEEECCCCCCEEEHHHHCCCCHHHHHHCCCCC
TMGYGLPAAMGAQAAFPERQVMVVCGDGGFQMNLQELATIVQNRLNVKICILNNNFLGMV
CCCCCCCHHHCCCCCCCCCEEEEEECCCCCEECHHHHHHHHHCCCCEEEEEECCCHHHHH
RQWQELFFDKRYSSTCMELPIDFIKLAEAFGATGLQATKVDEVDEVIKKGFATNGPVLME
HHHHHHHHHHHHCCHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEE
FKVAREEKVLPMVPAGASLTEMVLAS
EEECCCCCCCEECCCCCCHHHHHHCC
>Mature Secondary Structure
MKMNGARILLECLKREGVDTIFGYPGGTVINLYDELFSFKEIRHILPRHEQAGVHAADGY
CCCCHHHHHHHHHHHCCCCEEECCCCCCEEHHHHHHHHHHHHHHHCCCCHHCCCCCCCCC
ARATGKVGVAIATSGPGATNTITGIATAYMDSIPMVIVTGQVPTALIGNDAFQEADIIGI
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCCCCCEEEE
TRPCTKHNFLVKDVKDLPTIMKKAFYIARTGRPGPVLVDLPKDVQMAQAEFHYPETVEIR
CCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCCCHHHHHHHCCCCCEEEEE
GYKPNLEGHPKQVEKAVTMITQAKRPVLYVGGGVILGNAAAELTAFAKRLAIPVTTTLMG
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEECCHHHHHHHHHHHHCCCHHHHHHH
LGAFPENDTLSLGLLGMHGTYYANMAVSNCDVLIAIGARFDDRVTGKIASFAPHAKIVHV
CCCCCCCCCEEEEEEECCCEEEEEEEECCCCEEEEECCCCCCCHHCHHHHCCCCCEEEEE
DVDPTSIKKNVRVDLPIVGDVRDVLAKMLKAAELVCPDAKVCQDAIAPWTAEIEGWKAKH
ECCHHHHCCCCEEECCEECCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEECCCCCCCCC
PMSYKQSTTTIKPQYVIQRLRALSDPDAIVATDVGQHQMWTAQFFGFTTPRTLLSSGGLG
CCCCCCCCCEECHHHHHHHHHHCCCCCCEEEECCCCCCEEEHHHHCCCCHHHHHHCCCCC
TMGYGLPAAMGAQAAFPERQVMVVCGDGGFQMNLQELATIVQNRLNVKICILNNNFLGMV
CCCCCCCHHHCCCCCCCCCEEEEEECCCCCEECHHHHHHHHHCCCCEEEEEECCCHHHHH
RQWQELFFDKRYSSTCMELPIDFIKLAEAFGATGLQATKVDEVDEVIKKGFATNGPVLME
HHHHHHHHHHHHCCHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEE
FKVAREEKVLPMVPAGASLTEMVLAS
EEECCCCCCCEECCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9688822; 12089438 [H]