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The map label for this gene is ykfB [H]

Identifier: 197118796

GI number: 197118796

Start: 2802884

End: 2803990

Strand: Direct

Name: ykfB [H]

Synonym: Gbem_2417

Alternate gene names: 197118796

Gene position: 2802884-2803990 (Clockwise)

Preceding gene: 197118795

Following gene: 197118797

Centisome position: 60.73

GC content: 65.76

Gene sequence:

>1107_bases
ATGGATATTTCCTTTCAGATACAAGATGCCGTCGCCTCGGTGATACGGGCGCCGCTTGCCTCCCCTTTCAGGATCGCCAC
AGGGCAGCACGACGAACTGGAGAACGTGTTCCTCAAGCTCACCACGCGGGACGGCGTGTCGGGTTACGGCGAGGCCGCGG
TCGCCTCGCACATCACCGGCGAGACCGTGCCGGGAACGCTGGCGAACCTTCAAAACGCAGCGGCCGCCTTGCGCGGTCAA
ACCGTCGATGACGCGGAGAGCGCCTGCCGGCAGTTCGCGGCTGCCTTCGCCGGCAACCACGCAGGGCTCGCGGCGCTGGA
GATGGCGCTATTGGACCTCTCCTCGCGCGTGCGCGGCATCCCCTTTTACCGCCTCTTCGCCCCGGTGGCGGCCCTGGAGC
CGCGGCTCGCCTTCTCCACCGACATAACGGTCGTCATCGGCTCGCTGGACGAGGCCAGGGCCACCGCCCGGGAGTTCGCC
TCGCGCGGGTTCAAGGCCTTCAAGATCAAGATCGGCCGCGACGAGCAGCTCGACCTGGCACGCGTGCTTGCCGTGCACGA
GATAGCGCCGGATAGCCAGATCATCCTGGACGCCAACATGGGGTTCAGCGCCGGCTCCATGCTGGCCTTTCTGGACCGGC
TGGCCGCGAAAGGGGTGCGGCCGGTCCTTTTGGAACAGCCGGTGCCCAAAATGGATTGGGACGGTCTCAGCGAGATCACC
GCGGCGCTTACCGGAAGCGAAACACTCGTCTGCGCCGACGAGAGCGTAGGCTCCCTGGCCGACGCCCGGCGCGCCATCGA
CAGCAACGCGGTCAGCGCCATCAACGTGAAGTTCATGAAAAGCGGGATTCTGGAAGGAGCCGAGATCGCGAGGTTAGCCG
CCTCCCGCGGGATCCGGCTCATGCTGGGAGCGATGATGGAGAGCGCACTGGCGGTTACCGCGTCGGCTCACTTCGCAGCG
GGGCTCGCCTGCTTCGATTATCTGGATATGGACACCACCTTCTTCCTGAAGGGTGAGCTGGCCCATTCCCCCTACCTCGA
CGAGCACGGGCGCTTCGACCTGCACGACGCCGGCCCCGGCATCGGAGTCGAGCCCCGTTTTTCATGA

Upstream 100 bases:

>100_bases
AAGGGGTGAAGGGAGTGCTGCAAAAACCTTTCGACATGGACGCGCTGCGCAGGGTTATCAGGTAGGAGTTTTCCGGAGCC
ACATCCAAGGAGGCACAGAG

Downstream 100 bases:

>100_bases
TCAGCAACCCGGTTCTCATCGTAGCCGTCTTGATCTCCATCGAGGCCCTGGTCCTGTGGCTGTCGCGTCACGAGCGGACC
AGGCGCTATTTCGACCTGCT

Product: L-alanyl-D/L-glutamate epimerase

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MDISFQIQDAVASVIRAPLASPFRIATGQHDELENVFLKLTTRDGVSGYGEAAVASHITGETVPGTLANLQNAAAALRGQ
TVDDAESACRQFAAAFAGNHAGLAALEMALLDLSSRVRGIPFYRLFAPVAALEPRLAFSTDITVVIGSLDEARATAREFA
SRGFKAFKIKIGRDEQLDLARVLAVHEIAPDSQIILDANMGFSAGSMLAFLDRLAAKGVRPVLLEQPVPKMDWDGLSEIT
AALTGSETLVCADESVGSLADARRAIDSNAVSAINVKFMKSGILEGAEIARLAASRGIRLMLGAMMESALAVTASAHFAA
GLACFDYLDMDTTFFLKGELAHSPYLDEHGRFDLHDAGPGIGVEPRFS

Sequences:

>Translated_368_residues
MDISFQIQDAVASVIRAPLASPFRIATGQHDELENVFLKLTTRDGVSGYGEAAVASHITGETVPGTLANLQNAAAALRGQ
TVDDAESACRQFAAAFAGNHAGLAALEMALLDLSSRVRGIPFYRLFAPVAALEPRLAFSTDITVVIGSLDEARATAREFA
SRGFKAFKIKIGRDEQLDLARVLAVHEIAPDSQIILDANMGFSAGSMLAFLDRLAAKGVRPVLLEQPVPKMDWDGLSEIT
AALTGSETLVCADESVGSLADARRAIDSNAVSAINVKFMKSGILEGAEIARLAASRGIRLMLGAMMESALAVTASAHFAA
GLACFDYLDMDTTFFLKGELAHSPYLDEHGRFDLHDAGPGIGVEPRFS
>Mature_368_residues
MDISFQIQDAVASVIRAPLASPFRIATGQHDELENVFLKLTTRDGVSGYGEAAVASHITGETVPGTLANLQNAAAALRGQ
TVDDAESACRQFAAAFAGNHAGLAALEMALLDLSSRVRGIPFYRLFAPVAALEPRLAFSTDITVVIGSLDEARATAREFA
SRGFKAFKIKIGRDEQLDLARVLAVHEIAPDSQIILDANMGFSAGSMLAFLDRLAAKGVRPVLLEQPVPKMDWDGLSEIT
AALTGSETLVCADESVGSLADARRAIDSNAVSAINVKFMKSGILEGAEIARLAASRGIRLMLGAMMESALAVTASAHFAA
GLACFDYLDMDTTFFLKGELAHSPYLDEHGRFDLHDAGPGIGVEPRFS

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of the other Ala-X dipepti

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=321, Percent_Identity=27.7258566978193, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 38982; Mature: 38982

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDISFQIQDAVASVIRAPLASPFRIATGQHDELENVFLKLTTRDGVSGYGEAAVASHITG
CCCEEEHHHHHHHHHHHCCCCCEEECCCCCHHHCEEEEEEEECCCCCCCCCHHHHHHCCC
ETVPGTLANLQNAAAALRGQTVDDAESACRQFAAAFAGNHAGLAALEMALLDLSSRVRGI
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
PFYRLFAPVAALEPRLAFSTDITVVIGSLDEARATAREFASRGFKAFKIKIGRDEQLDLA
CHHHHHHHHHHHCCCCEECCCEEEEEECCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHH
RVLAVHEIAPDSQIILDANMGFSAGSMLAFLDRLAAKGVRPVLLEQPVPKMDWDGLSEIT
HHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCHHHHH
AALTGSETLVCADESVGSLADARRAIDSNAVSAINVKFMKSGILEGAEIARLAASRGIRL
HHHCCCCEEEECCCCHHHHHHHHHHHCCCCHHHEEHHHHHHCCCCHHHHHHHHHHCCHHH
MLGAMMESALAVTASAHFAAGLACFDYLDMDTTFFLKGELAHSPYLDEHGRFDLHDAGPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEEECCCCCC
IGVEPRFS
CCCCCCCC
>Mature Secondary Structure
MDISFQIQDAVASVIRAPLASPFRIATGQHDELENVFLKLTTRDGVSGYGEAAVASHITG
CCCEEEHHHHHHHHHHHCCCCCEEECCCCCHHHCEEEEEEEECCCCCCCCCHHHHHHCCC
ETVPGTLANLQNAAAALRGQTVDDAESACRQFAAAFAGNHAGLAALEMALLDLSSRVRGI
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
PFYRLFAPVAALEPRLAFSTDITVVIGSLDEARATAREFASRGFKAFKIKIGRDEQLDLA
CHHHHHHHHHHHCCCCEECCCEEEEEECCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHH
RVLAVHEIAPDSQIILDANMGFSAGSMLAFLDRLAAKGVRPVLLEQPVPKMDWDGLSEIT
HHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCHHHHH
AALTGSETLVCADESVGSLADARRAIDSNAVSAINVKFMKSGILEGAEIARLAASRGIRL
HHHCCCCEEEECCCCHHHHHHHHHHHCCCCHHHEEHHHHHHCCCCHHHHHHHHHHCCHHH
MLGAMMESALAVTASAHFAAGLACFDYLDMDTTFFLKGELAHSPYLDEHGRFDLHDAGPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEEECCCCCC
IGVEPRFS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]