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The map label for this gene is purM

Identifier: 197118781

GI number: 197118781

Start: 2781267

End: 2782313

Strand: Direct

Name: purM

Synonym: Gbem_2402

Alternate gene names: 197118781

Gene position: 2781267-2782313 (Clockwise)

Preceding gene: 197118774

Following gene: 197118782

Centisome position: 60.26

GC content: 60.94

Gene sequence:

>1047_bases
TTGAAAGAGACTAAGATTACCTATAAAGACGCAGGTGTAGACATAGATGCAGGCAACACTTTCGTGCAGATGATCAAGCC
GCTGGTCAAGGCGACTTCTCGTCCGGAAGTGCTGGCAGACATCGGCGGCTTCGGGGGGTTGTTTTCCCTCAACATGGGCA
AGTACAAGCACCCGGTGCTTGTCTCCGGCACGGACGGGGTCGGGACCAAACTGAAGCTCGCCTTCCTCGCCGACCGCCAT
GACACCATCGGCATCGACCTCGTCGCCATGTGCGTGAACGACATCATCGTGCAGGGAGCCGAGCCGCTCTTCTTCCTCGA
TTATCTTGCTACCGCGAAGCTCGACCCGGCCAAGGCCGCCTCCATCATCAAAGGTGTGTCCGAGGGTTGCGTGCAGGCGG
GGTGCGCCCTGATCGGCGGCGAAACCGCCGAGATGCCCGGCTTCTACACCGGCGACGAGTACGACATGGCCGGCTTTGCC
GTGGGCGTGGTGGAGCGCGAAAAAATCATCGACGGCTCCTCCATCACCGTCGGCAACCGCCTGATCGGGCTGGCCTCCTC
CGGGCTGCACAGCAACGGCTACTCCCTGGCCAGGAAGGTCATCCTGGAGCACATGGGGCTCGGCATCGACGATCAACTCC
CCGGCCTTGGGAAAACCGTCGCCGAGGAACTTCTCACTCCGACCCGCATCTACGTGCGCAGCGTGATGAACCTTCTGCGC
GACTTCAACGTCTCGGGCCTGGCACACATCACCGGCGGCGGTCTCCTGGAGAACATCCCCCGCGTGCTCCCCAACGGCTG
CAAGGCCGTCATCAAGAAGGACAGCTGGGACGTCCCCGAGATATTCCGGATCATGCAGAAGGCCGGCAACATCGAGGAAA
ACGAGATGTTCAGGACCTTCAACTGCGGCATCGGCATGGTGCTGGTCGTTCCCGAGAAAGAGTCCGAAGAAATCATGATC
AGGCTCTCCGGGCTAAACGAGACCGCGTTCGTCATCGGCGAAGTGGCTAAGTGCGACGCCGGCAAGGAGTGCGTGGACCT
CGTCTAG

Upstream 100 bases:

>100_bases
CATCATAAGCAAACGCCAAGGCGAAGTAAACAATTTTTTCGCTTTGACTTTTCGAGGTATATGGTTTAGATTTCGTCTTT
ACTTTATCGGAGGTTCTCCC

Downstream 100 bases:

>100_bases
GGTAAAGCGTCAGATATGGAAAGAACTCTCAACATAGGGGTACTGATCTCCGGCAGCGGCAGCAACCTGCAGTCGATCAT
GGACGCCTGCGCCGCCGGCA

Product: phosphoribosylaminoimidazole synthetase

Products: NA

Alternate protein names: AIR synthase; AIRS; Phosphoribosyl-aminoimidazole synthetase

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKETKITYKDAGVDIDAGNTFVQMIKPLVKATSRPEVLADIGGFGGLFSLNMGKYKHPVLVSGTDGVGTKLKLAFLADRH
DTIGIDLVAMCVNDIIVQGAEPLFFLDYLATAKLDPAKAASIIKGVSEGCVQAGCALIGGETAEMPGFYTGDEYDMAGFA
VGVVEREKIIDGSSITVGNRLIGLASSGLHSNGYSLARKVILEHMGLGIDDQLPGLGKTVAEELLTPTRIYVRSVMNLLR
DFNVSGLAHITGGGLLENIPRVLPNGCKAVIKKDSWDVPEIFRIMQKAGNIEENEMFRTFNCGIGMVLVVPEKESEEIMI
RLSGLNETAFVIGEVAKCDAGKECVDLV

Sequences:

>Translated_348_residues
MKETKITYKDAGVDIDAGNTFVQMIKPLVKATSRPEVLADIGGFGGLFSLNMGKYKHPVLVSGTDGVGTKLKLAFLADRH
DTIGIDLVAMCVNDIIVQGAEPLFFLDYLATAKLDPAKAASIIKGVSEGCVQAGCALIGGETAEMPGFYTGDEYDMAGFA
VGVVEREKIIDGSSITVGNRLIGLASSGLHSNGYSLARKVILEHMGLGIDDQLPGLGKTVAEELLTPTRIYVRSVMNLLR
DFNVSGLAHITGGGLLENIPRVLPNGCKAVIKKDSWDVPEIFRIMQKAGNIEENEMFRTFNCGIGMVLVVPEKESEEIMI
RLSGLNETAFVIGEVAKCDAGKECVDLV
>Mature_348_residues
MKETKITYKDAGVDIDAGNTFVQMIKPLVKATSRPEVLADIGGFGGLFSLNMGKYKHPVLVSGTDGVGTKLKLAFLADRH
DTIGIDLVAMCVNDIIVQGAEPLFFLDYLATAKLDPAKAASIIKGVSEGCVQAGCALIGGETAEMPGFYTGDEYDMAGFA
VGVVEREKIIDGSSITVGNRLIGLASSGLHSNGYSLARKVILEHMGLGIDDQLPGLGKTVAEELLTPTRIYVRSVMNLLR
DFNVSGLAHITGGGLLENIPRVLPNGCKAVIKKDSWDVPEIFRIMQKAGNIEENEMFRTFNCGIGMVLVVPEKESEEIMI
RLSGLNETAFVIGEVAKCDAGKECVDLV

Specific function: De novo purine biosynthesis; fifth step. [C]

COG id: COG0150

COG function: function code F; Phosphoribosylaminoimidazole (AIR) synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AIR synthase family

Homologues:

Organism=Homo sapiens, GI4503915, Length=333, Percent_Identity=53.4534534534535, Blast_Score=355, Evalue=3e-98,
Organism=Homo sapiens, GI209869995, Length=333, Percent_Identity=53.4534534534535, Blast_Score=355, Evalue=3e-98,
Organism=Homo sapiens, GI209869993, Length=333, Percent_Identity=53.4534534534535, Blast_Score=355, Evalue=3e-98,
Organism=Escherichia coli, GI1788845, Length=344, Percent_Identity=58.1395348837209, Blast_Score=401, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17567511, Length=343, Percent_Identity=45.7725947521866, Blast_Score=263, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6321203, Length=337, Percent_Identity=48.0712166172107, Blast_Score=317, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24582400, Length=295, Percent_Identity=46.1016949152542, Blast_Score=242, Evalue=2e-64,

Paralogues:

None

Copy number: 180 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR5_GEOBB (B5EFU7)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002139208.1
- ProteinModelPortal:   B5EFU7
- SMR:   B5EFU7
- GeneID:   6782400
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_2402
- HOGENOM:   HBG531222
- OMA:   HYDLAGF
- ProtClustDB:   PRK05385
- GO:   GO:0005737
- HAMAP:   MF_00741_B
- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR004733
- InterPro:   IPR016188
- TIGRFAMs:   TIGR00878

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C; SSF56042 AIR_synth_C; SSF55326 PurM_N-like

EC number: =6.3.3.1

Molecular weight: Translated: 37201; Mature: 37201

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKETKITYKDAGVDIDAGNTFVQMIKPLVKATSRPEVLADIGGFGGLFSLNMGKYKHPVL
CCCCEEEEECCCEEECCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEECCCCCCCCEE
VSGTDGVGTKLKLAFLADRHDTIGIDLVAMCVNDIIVQGAEPLFFLDYLATAKLDPAKAA
EECCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHH
SIIKGVSEGCVQAGCALIGGETAEMPGFYTGDEYDMAGFAVGVVEREKIIDGSSITVGNR
HHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHCCCCCCEEECCC
LIGLASSGLHSNGYSLARKVILEHMGLGIDDQLPGLGKTVAEELLTPTRIYVRSVMNLLR
EEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHH
DFNVSGLAHITGGGLLENIPRVLPNGCKAVIKKDSWDVPEIFRIMQKAGNIEENEMFRTF
HCCCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCHHHEEEEE
NCGIGMVLVVPEKESEEIMIRLSGLNETAFVIGEVAKCDAGKECVDLV
CCCCEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCHHHHCC
>Mature Secondary Structure
MKETKITYKDAGVDIDAGNTFVQMIKPLVKATSRPEVLADIGGFGGLFSLNMGKYKHPVL
CCCCEEEEECCCEEECCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEECCCCCCCCEE
VSGTDGVGTKLKLAFLADRHDTIGIDLVAMCVNDIIVQGAEPLFFLDYLATAKLDPAKAA
EECCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHH
SIIKGVSEGCVQAGCALIGGETAEMPGFYTGDEYDMAGFAVGVVEREKIIDGSSITVGNR
HHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHCCCCCCEEECCC
LIGLASSGLHSNGYSLARKVILEHMGLGIDDQLPGLGKTVAEELLTPTRIYVRSVMNLLR
EEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHH
DFNVSGLAHITGGGLLENIPRVLPNGCKAVIKKDSWDVPEIFRIMQKAGNIEENEMFRTF
HCCCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCHHHEEEEE
NCGIGMVLVVPEKESEEIMIRLSGLNETAFVIGEVAKCDAGKECVDLV
CCCCEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA