Click here to switch to the map view.

The map label for this gene is bioD [C]

Identifier: 197118774

GI number: 197118774

Start: 2775834

End: 2776925

Strand: Direct

Name: bioD [C]

Synonym: Gbem_2395

Alternate gene names: 197118774

Gene position: 2775834-2776925 (Clockwise)

Preceding gene: 197118773

Following gene: 197118781

Centisome position: 60.15

GC content: 56.59

Gene sequence:

>1092_bases
ATGGCGAAAAAGATCTTCGTTGCGGCGACCGGGCAAAACTGTGGCAAGACGACCATCAGCATCTCGCTCATGCACCAGGC
CAGGAAAAAGTACCGTCGAGTCGGCTTTGTAAAGCCTTTCGGGCCCAAAGTGCTCCTTTATGACGACTTCATGGTGGACA
TGGACGCTCTGCTGATGGCGAAAGCATTCGGCATGGAAGACGACATCGCCCTCATGTCTCCTGTGGCGCTGCACCGGGAT
TTCACCAAGGATTACCTGAGCGGCAAACTCGACGACCTGTCGCTGGCCGATTGCGTGGTGGAGGCGGTGCGGGAGTTGGA
GGAGAAATACGACTTCCTCATCATCGAGGGAGCGGGGCACGGCGGGGTCGGCTCGGTGATCGGGCTGAGCAACGCTAAAG
TGGCCAAGCTGATCGACGCTCCGGTCATGATCGTCACCGAAAGCGGCATCGGCAAGGTGATCGATGCGGTGCATTTGAAC
CTGGCGCTGTACCAGCGCGAAGGGGCCGATGTGCGGGGGATCATCGTCAACAAGATGCTTCCCGGGAAGAAGGAACTCAC
CAACAAGTACCTCAAACAAGCCTTTGAACCTATCAACGTCAAGGTGACCGACGGTTTCAACTACTCCCCCATCCTGGCCA
ACCCGACGCTAAGCCACATCTCGAAGTTGTTCGACCTGCCGCTGCACGGCGACATCAAAGAAAAAAGCCGCATCATCCAC
AACATCCAGTTGGGAGCGGCTTCTTCGCAACGTGTGATCGACGGACTGCTTGACTCGACCTTGATGATAGTCACGAGCTC
CAGAGACGAGCTGATCGTTACCCTATCCTCCCTGTACCACATCCCCTCCTACAGAGAGAAGATCGCCGGCCTCGTAGTAT
CCGGGCACTGCGCCGTTTCCGACATCAGCCAGCAGATCCTCAACGATTCCGGCATCCCCTACATCAGGGTGGAAGAAACC
ACCGCCGAGGTGTTCACCGCTCTTTCCGACGACGTTGCCAAGATCACCTTCGAGGACCAGGAGAAGCTGAACTGGATTAT
GGCCAACGCCGAAAAGGGCATGGATTTCGAAGCAATAGACGCGCTGCTGTAA

Upstream 100 bases:

>100_bases
GGACATGGCCGCCCAGCGCGGCATTTCCCGTTCCTCCCACTGCGCTCGCGCGCAGGAGGGATTATGAGTTGTGGATCGGC
ATTTAAACGAGGGGTATGGC

Downstream 100 bases:

>100_bases
GGCATGTAAGGCGGTTCAACGTTCGACGTTCAACGTTAAAACCTGTCCCCTAGCCCCTAGCCCCTGATTCTCGCCCCCTA
GCCCCTGCTTCAAAGCGTAG

Product: BioD/DRTGG domain-containing protein

Products: ADP; Dethiobiotin; H+; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MAKKIFVAATGQNCGKTTISISLMHQARKKYRRVGFVKPFGPKVLLYDDFMVDMDALLMAKAFGMEDDIALMSPVALHRD
FTKDYLSGKLDDLSLADCVVEAVRELEEKYDFLIIEGAGHGGVGSVIGLSNAKVAKLIDAPVMIVTESGIGKVIDAVHLN
LALYQREGADVRGIIVNKMLPGKKELTNKYLKQAFEPINVKVTDGFNYSPILANPTLSHISKLFDLPLHGDIKEKSRIIH
NIQLGAASSQRVIDGLLDSTLMIVTSSRDELIVTLSSLYHIPSYREKIAGLVVSGHCAVSDISQQILNDSGIPYIRVEET
TAEVFTALSDDVAKITFEDQEKLNWIMANAEKGMDFEAIDALL

Sequences:

>Translated_363_residues
MAKKIFVAATGQNCGKTTISISLMHQARKKYRRVGFVKPFGPKVLLYDDFMVDMDALLMAKAFGMEDDIALMSPVALHRD
FTKDYLSGKLDDLSLADCVVEAVRELEEKYDFLIIEGAGHGGVGSVIGLSNAKVAKLIDAPVMIVTESGIGKVIDAVHLN
LALYQREGADVRGIIVNKMLPGKKELTNKYLKQAFEPINVKVTDGFNYSPILANPTLSHISKLFDLPLHGDIKEKSRIIH
NIQLGAASSQRVIDGLLDSTLMIVTSSRDELIVTLSSLYHIPSYREKIAGLVVSGHCAVSDISQQILNDSGIPYIRVEET
TAEVFTALSDDVAKITFEDQEKLNWIMANAEKGMDFEAIDALL
>Mature_362_residues
AKKIFVAATGQNCGKTTISISLMHQARKKYRRVGFVKPFGPKVLLYDDFMVDMDALLMAKAFGMEDDIALMSPVALHRDF
TKDYLSGKLDDLSLADCVVEAVRELEEKYDFLIIEGAGHGGVGSVIGLSNAKVAKLIDAPVMIVTESGIGKVIDAVHLNL
ALYQREGADVRGIIVNKMLPGKKELTNKYLKQAFEPINVKVTDGFNYSPILANPTLSHISKLFDLPLHGDIKEKSRIIHN
IQLGAASSQRVIDGLLDSTLMIVTSSRDELIVTLSSLYHIPSYREKIAGLVVSGHCAVSDISQQILNDSGIPYIRVEETT
AEVFTALSDDVAKITFEDQEKLNWIMANAEKGMDFEAIDALL

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0857

COG function: function code R; BioD-like N-terminal domain of phosphotransacetylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To Synechocystis PCC 6803 slr0039 [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010766 [H]

Pfam domain/function: PF07085 DRTGG [H]

EC number: 6.3.3.3 [C]

Molecular weight: Translated: 39831; Mature: 39699

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKIFVAATGQNCGKTTISISLMHQARKKYRRVGFVKPFGPKVLLYDDFMVDMDALLMA
CCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHHH
KAFGMEDDIALMSPVALHRDFTKDYLSGKLDDLSLADCVVEAVRELEEKYDFLIIEGAGH
HHCCCCCCHHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCC
GGVGSVIGLSNAKVAKLIDAPVMIVTESGIGKVIDAVHLNLALYQREGADVRGIIVNKML
CCCCCEEECCCCHHHHHHCCCEEEEECCCCHHHHHHHHHEEEEEECCCCCEEEEEEECCC
PGKKELTNKYLKQAFEPINVKVTDGFNYSPILANPTLSHISKLFDLPLHGDIKEKSRIIH
CCHHHHHHHHHHHHCCCCEEEEECCCCCCCEECCCHHHHHHHHHCCCCCCCHHHHHHHHH
NIQLGAASSQRVIDGLLDSTLMIVTSSRDELIVTLSSLYHIPSYREKIAGLVVSGHCAVS
EEEECCCCHHHHHHHHHCCEEEEEECCCCCEEEEEHHHHCCCCHHHHHCCEEEECCCHHH
DISQQILNDSGIPYIRVEETTAEVFTALSDDVAKITFEDQEKLNWIMANAEKGMDFEAID
HHHHHHHCCCCCCEEEEECHHHHHHHHHCCCCEEEEECCHHHCEEEEECCCCCCCHHHHH
ALL
HCC
>Mature Secondary Structure 
AKKIFVAATGQNCGKTTISISLMHQARKKYRRVGFVKPFGPKVLLYDDFMVDMDALLMA
CCEEEEEECCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHHH
KAFGMEDDIALMSPVALHRDFTKDYLSGKLDDLSLADCVVEAVRELEEKYDFLIIEGAGH
HHCCCCCCHHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCC
GGVGSVIGLSNAKVAKLIDAPVMIVTESGIGKVIDAVHLNLALYQREGADVRGIIVNKML
CCCCCEEECCCCHHHHHHCCCEEEEECCCCHHHHHHHHHEEEEEECCCCCEEEEEEECCC
PGKKELTNKYLKQAFEPINVKVTDGFNYSPILANPTLSHISKLFDLPLHGDIKEKSRIIH
CCHHHHHHHHHHHHCCCCEEEEECCCCCCCEECCCHHHHHHHHHCCCCCCCHHHHHHHHH
NIQLGAASSQRVIDGLLDSTLMIVTSSRDELIVTLSSLYHIPSYREKIAGLVVSGHCAVS
EEEECCCCHHHHHHHHHCCEEEEEECCCCCEEEEEHHHHCCCCHHHHHCCEEEECCCHHH
DISQQILNDSGIPYIRVEETTAEVFTALSDDVAKITFEDQEKLNWIMANAEKGMDFEAID
HHHHHHHCCCCCCEEEEECHHHHHHHHHCCCCEEEEECCHHHCEEEEECCCCCCCHHHHH
ALL
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10 {HCO3-}} 40 {MgCl2}} 0.045 {7,8-diaminononanoate}} 0.42 {ATP}} [C]

Substrates: ATP; CO2; 7,8-Diaminononanoate [C]

Specific reaction: ATP + CO2 + 7,8-Diaminononanoate <==> ADP + Dethiobiotin + (3) H+ + Phosphate [C]

General reaction: Carboxylation; Heteroatomic ring closure [C]

Inhibitor: Ag+; Cd2+; Co2+; Cu2+; Hg2+; Ni2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]