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The map label for this gene is purN [H]

Identifier: 197118782

GI number: 197118782

Start: 2782329

End: 2782943

Strand: Direct

Name: purN [H]

Synonym: Gbem_2403

Alternate gene names: 197118782

Gene position: 2782329-2782943 (Clockwise)

Preceding gene: 197118781

Following gene: 197118785

Centisome position: 60.29

GC content: 66.67

Gene sequence:

>615_bases
ATGGAAAGAACTCTCAACATAGGGGTACTGATCTCCGGCAGCGGCAGCAACCTGCAGTCGATCATGGACGCCTGCGCCGC
CGGCAGGATCAAGGGCAGGGTCGCCTGCGTGATCAGCAACAAGGCGGACGCCTTCGGCCTGGAGCGGGCGCGCAAGGCCG
GCATCCCCGCGCTGCACCTGGATCACCGCGCCTACTCCGGCAGGGAGTCCTACGACGAGGCCCTGGTGGCGACGCTGCGC
GAATTCGACGTCGAGCTGGTGGCGCTGGCAGGCTTCATGCGCATCATCACGCCGGTGCTACTCGAGGCCTTCCCGATGGC
GGTGATGAACATCCATCCGGCCCTGCTCCCTGCCTTTCCCGGGCTGCACGCCCAGCGCCAGGCGCTCGATTACGGCGCCA
AGGTCGCCGGCTGCACCGTCCATTTCGTCGACCCGGGCACCGACACGGGCCCGATCATAATGCAGGCCGCGGTGCCGGTC
CTTCCAAGCGACACCGAGCAGACCCTCTCGGCACGGATTCAGAAGGAAGAGCACCGCCTCTATCCTGAGGCGATCCGGCT
CTTCACCGAGGGGCTCCTAGAGGTCAGCGGTCGCGTAGTCAGCGTCGGCGCCTGA

Upstream 100 bases:

>100_bases
GCTCTCCGGGCTAAACGAGACCGCGTTCGTCATCGGCGAAGTGGCTAAGTGCGACGCCGGCAAGGAGTGCGTGGACCTCG
TCTAGGGTAAAGCGTCAGAT

Downstream 100 bases:

>100_bases
TTCCCCGACAACTTCGCCTAAAACAAAATCCCCTTCGGTAGCCGAGGGGGATTTTTTTATGCCTTAAACCTGTTGATTGT
TTTCCCTAAGCCGTCACCGG

Product: phosphoribosylglycinamide formyltransferase, folate-dependent

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MERTLNIGVLISGSGSNLQSIMDACAAGRIKGRVACVISNKADAFGLERARKAGIPALHLDHRAYSGRESYDEALVATLR
EFDVELVALAGFMRIITPVLLEAFPMAVMNIHPALLPAFPGLHAQRQALDYGAKVAGCTVHFVDPGTDTGPIIMQAAVPV
LPSDTEQTLSARIQKEEHRLYPEAIRLFTEGLLEVSGRVVSVGA

Sequences:

>Translated_204_residues
MERTLNIGVLISGSGSNLQSIMDACAAGRIKGRVACVISNKADAFGLERARKAGIPALHLDHRAYSGRESYDEALVATLR
EFDVELVALAGFMRIITPVLLEAFPMAVMNIHPALLPAFPGLHAQRQALDYGAKVAGCTVHFVDPGTDTGPIIMQAAVPV
LPSDTEQTLSARIQKEEHRLYPEAIRLFTEGLLEVSGRVVSVGA
>Mature_204_residues
MERTLNIGVLISGSGSNLQSIMDACAAGRIKGRVACVISNKADAFGLERARKAGIPALHLDHRAYSGRESYDEALVATLR
EFDVELVALAGFMRIITPVLLEAFPMAVMNIHPALLPAFPGLHAQRQALDYGAKVAGCTVHFVDPGTDTGPIIMQAAVPV
LPSDTEQTLSARIQKEEHRLYPEAIRLFTEGLLEVSGRVVSVGA

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=203, Percent_Identity=41.871921182266, Blast_Score=175, Evalue=2e-44,
Organism=Homo sapiens, GI209869995, Length=203, Percent_Identity=41.871921182266, Blast_Score=175, Evalue=2e-44,
Organism=Homo sapiens, GI209869993, Length=203, Percent_Identity=41.871921182266, Blast_Score=175, Evalue=2e-44,
Organism=Homo sapiens, GI21614513, Length=158, Percent_Identity=32.9113924050633, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1788846, Length=191, Percent_Identity=50.261780104712, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1787483, Length=163, Percent_Identity=29.4478527607362, Blast_Score=84, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=192, Percent_Identity=40.1041666666667, Blast_Score=150, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6320616, Length=195, Percent_Identity=28.7179487179487, Blast_Score=73, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24582400, Length=202, Percent_Identity=48.019801980198, Blast_Score=196, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 21859; Mature: 21859

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERTLNIGVLISGSGSNLQSIMDACAAGRIKGRVACVISNKADAFGLERARKAGIPALHL
CCCEEEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEE
DHRAYSGRESYDEALVATLREFDVELVALAGFMRIITPVLLEAFPMAVMNIHPALLPAFP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCC
GLHAQRQALDYGAKVAGCTVHFVDPGTDTGPIIMQAAVPVLPSDTEQTLSARIQKEEHRL
CCHHHHHHHHCCCEEECCEEEEECCCCCCCCEEEEHHCCCCCCCHHHHHHHHHHHHHHHC
YPEAIRLFTEGLLEVSGRVVSVGA
CHHHHHHHHHHHHHHCCEEEEECC
>Mature Secondary Structure
MERTLNIGVLISGSGSNLQSIMDACAAGRIKGRVACVISNKADAFGLERARKAGIPALHL
CCCEEEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEE
DHRAYSGRESYDEALVATLREFDVELVALAGFMRIITPVLLEAFPMAVMNIHPALLPAFP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCC
GLHAQRQALDYGAKVAGCTVHFVDPGTDTGPIIMQAAVPVLPSDTEQTLSARIQKEEHRL
CCHHHHHHHHCCCEEECCEEEEECCCCCCCCEEEEHHCCCCCCCHHHHHHHHHHHHHHHC
YPEAIRLFTEGLLEVSGRVVSVGA
CHHHHHHHHHHHHHHCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]