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The map label for this gene is cysD-1

Identifier: 197118734

GI number: 197118734

Start: 2730619

End: 2731524

Strand: Reverse

Name: cysD-1

Synonym: Gbem_2353

Alternate gene names: 197118734

Gene position: 2731524-2730619 (Counterclockwise)

Preceding gene: 197118735

Following gene: 197118733

Centisome position: 59.19

GC content: 61.48

Gene sequence:

>906_bases
ATGACCACACAATTGACCCATCTGCAGCAACTCGAAGCGGAAAGCATCCACATCATCCGTGAAGTGGTAGCCGAATTCGA
CAACCCGGTGATGCTGTACTCCATCGGCAAGGACTCGGCCGTCATGCTCCATCTAGCCCGCAAGGCCTTTTATCCTGCTC
CGCCGCCGTTTCCCTTGCTGCACGTGGACACCACCTGGAAGTTCCGTGAAATGATCCAGTTCCGCGGCCGGATGGCCAAA
GAGTCCGGCATGGAGCTCCTGGTGCACGTGAACGAGGACGGCGTAAAGAGGGGGGTCAGTCCCTTCACCCACGGCTCCGC
GCTTTACACCGACGTGATGAAGACCGAAGGGTTGAAGCAGGCGCTCGACCACTACAAGTTCGACGCTGCCTTCGGAGGCG
CCCGCCGCGACGAGGAGAAGTCCCGCGCCAAGGAGCGTATCTTCTCCTTCCGCAGCGCGAACCACCGCTGGGACCCGAAG
AACCAGCGCCCCGAGCTCTGGAGCCTCTACAATACCCGCATCAAGCCGGGCGAGAGCATCCGCGTTTTCCCGCTCTCCAA
CTGGACCGAGCTCGACATCTGGCAGTACATCCACCTGGAGCAGATCCCGATCGTTCCCCTTTACTACGCCGCCGTCAGGC
CCGTGGTTGAGCGCGACGGCATGCTGATCATGGTGGACGACGACCGGCTGGAGCTTAAGCCCGGCGAGAAGGTGGAGCAG
AAATCGGTCCGCTTCCGCACCCTGGGATGCTACCCCCTTACGGGCGCGGTCGAATCCGAGGCCGACACTCTTCCCGAAAT
CATCCAGGAAATGCTCCTCACCCGCACCTCCGAGCGCCAGGGGCGCCTGATCGACCACGACCAGGCCGGCTCCATGGAGA
AGAAGAAACAGGAGGGGTACTTCTAA

Upstream 100 bases:

>100_bases
AAGAGTGCGGGTTGCACAGAAGATAGGACGCTAACGACAGGGGGGATTGCCCGGTGGCAGCATCGGGCAGCCGTTACACA
GCTTTGAAATGGGAACTCAT

Downstream 100 bases:

>100_bases
TGGCACATCAATCGGAACTGATCGAGAAGGACATCCTCGCCTACCTGAAGAGTCAGGAGGAGAAATCCCTGCTCCGTTTC
ATCACCTGCGGCAGCGTGGA

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MTTQLTHLQQLEAESIHIIREVVAEFDNPVMLYSIGKDSAVMLHLARKAFYPAPPPFPLLHVDTTWKFREMIQFRGRMAK
ESGMELLVHVNEDGVKRGVSPFTHGSALYTDVMKTEGLKQALDHYKFDAAFGGARRDEEKSRAKERIFSFRSANHRWDPK
NQRPELWSLYNTRIKPGESIRVFPLSNWTELDIWQYIHLEQIPIVPLYYAAVRPVVERDGMLIMVDDDRLELKPGEKVEQ
KSVRFRTLGCYPLTGAVESEADTLPEIIQEMLLTRTSERQGRLIDHDQAGSMEKKKQEGYF

Sequences:

>Translated_301_residues
MTTQLTHLQQLEAESIHIIREVVAEFDNPVMLYSIGKDSAVMLHLARKAFYPAPPPFPLLHVDTTWKFREMIQFRGRMAK
ESGMELLVHVNEDGVKRGVSPFTHGSALYTDVMKTEGLKQALDHYKFDAAFGGARRDEEKSRAKERIFSFRSANHRWDPK
NQRPELWSLYNTRIKPGESIRVFPLSNWTELDIWQYIHLEQIPIVPLYYAAVRPVVERDGMLIMVDDDRLELKPGEKVEQ
KSVRFRTLGCYPLTGAVESEADTLPEIIQEMLLTRTSERQGRLIDHDQAGSMEKKKQEGYF
>Mature_300_residues
TTQLTHLQQLEAESIHIIREVVAEFDNPVMLYSIGKDSAVMLHLARKAFYPAPPPFPLLHVDTTWKFREMIQFRGRMAKE
SGMELLVHVNEDGVKRGVSPFTHGSALYTDVMKTEGLKQALDHYKFDAAFGGARRDEEKSRAKERIFSFRSANHRWDPKN
QRPELWSLYNTRIKPGESIRVFPLSNWTELDIWQYIHLEQIPIVPLYYAAVRPVVERDGMLIMVDDDRLELKPGEKVEQK
SVRFRTLGCYPLTGAVESEADTLPEIIQEMLLTRTSERQGRLIDHDQAGSMEKKKQEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily

Homologues:

Organism=Escherichia coli, GI1789109, Length=298, Percent_Identity=76.1744966442953, Blast_Score=476, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSD_GEOBB (B5EF56)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002139161.1
- ProteinModelPortal:   B5EF56
- SMR:   B5EF56
- GeneID:   6782352
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_2353
- HOGENOM:   HBG758022
- OMA:   GMIRFRT
- ProtClustDB:   PRK05253
- HAMAP:   MF_00064
- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF002936
- TIGRFAMs:   TIGR02039

Pfam domain/function: PF01507 PAPS_reduct

EC number: =2.7.7.4

Molecular weight: Translated: 34848; Mature: 34717

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQLTHLQQLEAESIHIIREVVAEFDNPVMLYSIGKDSAVMLHLARKAFYPAPPPFPLL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCEE
HVDTTWKFREMIQFRGRMAKESGMELLVHVNEDGVKRGVSPFTHGSALYTDVMKTEGLKQ
EECCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHCCCCCCCCHHHHHHHHHHHHHHH
ALDHYKFDAAFGGARRDEEKSRAKERIFSFRSANHRWDPKNQRPELWSLYNTRIKPGESI
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCE
RVFPLSNWTELDIWQYIHLEQIPIVPLYYAAVRPVVERDGMLIMVDDDRLELKPGEKVEQ
EEEECCCCCEEHHHHHEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEECCCCCHHHH
KSVRFRTLGCYPLTGAVESEADTLPEIIQEMLLTRTSERQGRLIDHDQAGSMEKKKQEGY
HCCEEEEECCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCC
F
C
>Mature Secondary Structure 
TTQLTHLQQLEAESIHIIREVVAEFDNPVMLYSIGKDSAVMLHLARKAFYPAPPPFPLL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCEE
HVDTTWKFREMIQFRGRMAKESGMELLVHVNEDGVKRGVSPFTHGSALYTDVMKTEGLKQ
EECCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHCCCCCCCCHHHHHHHHHHHHHHH
ALDHYKFDAAFGGARRDEEKSRAKERIFSFRSANHRWDPKNQRPELWSLYNTRIKPGESI
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCE
RVFPLSNWTELDIWQYIHLEQIPIVPLYYAAVRPVVERDGMLIMVDDDRLELKPGEKVEQ
EEEECCCCCEEHHHHHEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEECCCCCHHHH
KSVRFRTLGCYPLTGAVESEADTLPEIIQEMLLTRTSERQGRLIDHDQAGSMEKKKQEGY
HCCEEEEECCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA