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The map label for this gene is apr [H]

Identifier: 197118735

GI number: 197118735

Start: 2731599

End: 2732291

Strand: Reverse

Name: apr [H]

Synonym: Gbem_2354

Alternate gene names: 197118735

Gene position: 2732291-2731599 (Counterclockwise)

Preceding gene: 197118736

Following gene: 197118734

Centisome position: 59.2

GC content: 61.47

Gene sequence:

>693_bases
ATGAAATCTGTTCCTGAAGTCCCCTCGAACGCATCCCCGCAGGATATTCTTCGGCTCGGCTTGGAAGCCGCGGAAGGCCC
GGTTAAACTGGCGTGCTCCTTTTCGTTGGAAGACGTCGCCATCATCGACCTGGCCAAAGCTGCCGGCCTGGAGATCGGCG
TCTTCGCGCTGGATACCGGAAGGCTCAACGAGGAGACTTACGAGGTTGCCGACGCCATCTCGCAGCGCTTCGGCATCTCC
ATCGACTGGTACTTCCCGCGGCATGAAGCGGTCGAAAAACTGGAAAGGGAAAAAGGGCTTTTCTCGTTCAGGGAATCGCT
CGATAACCGCCACGAGTGCTGCCACATCAGAAAGGTGGAACCGCTGGGCCGGGCCCTTCAGGGACTCTCCGGGTGGATCA
CCGGGATGCGCAGGGAGCACAACGTGACCCGCATCGACCTGAAATCGATCGAGCTCGACCAGTTGAACGGCGGCATCCTC
AAGTTGAATCCGCTGCTGGACTGGAACGAGGAACAGCTTCTCGCGTACGTGCAGCAGCAGCGCCTGCCGCAGAACCGCCT
TCTGAAAGAGGGTTACCGCTCCATCGGCTGCGCCCCCTGCACGCGCGCCGTGCAGCCGGGGGAGGACGCTCGGGCCGGCC
GCTGGTGGTGGGAGAACCCGGAGCACAAAGAGTGCGGGTTGCACAGAAGATAG

Upstream 100 bases:

>100_bases
TTTTCTTGACTGCTTTGGTTATATATTGTACAAGGTGGACAACGAAATTAATGGGTGTGGCGATCGCCGCTCCTGTAACG
ACCTTGCAAAGGAAGCCGCT

Downstream 100 bases:

>100_bases
GACGCTAACGACAGGGGGGATTGCCCGGTGGCAGCATCGGGCAGCCGTTACACAGCTTTGAAATGGGAACTCATATGACC
ACACAATTGACCCATCTGCA

Product: adenosine 5'-phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MKSVPEVPSNASPQDILRLGLEAAEGPVKLACSFSLEDVAIIDLAKAAGLEIGVFALDTGRLNEETYEVADAISQRFGIS
IDWYFPRHEAVEKLEREKGLFSFRESLDNRHECCHIRKVEPLGRALQGLSGWITGMRREHNVTRIDLKSIELDQLNGGIL
KLNPLLDWNEEQLLAYVQQQRLPQNRLLKEGYRSIGCAPCTRAVQPGEDARAGRWWWENPEHKECGLHRR

Sequences:

>Translated_230_residues
MKSVPEVPSNASPQDILRLGLEAAEGPVKLACSFSLEDVAIIDLAKAAGLEIGVFALDTGRLNEETYEVADAISQRFGIS
IDWYFPRHEAVEKLEREKGLFSFRESLDNRHECCHIRKVEPLGRALQGLSGWITGMRREHNVTRIDLKSIELDQLNGGIL
KLNPLLDWNEEQLLAYVQQQRLPQNRLLKEGYRSIGCAPCTRAVQPGEDARAGRWWWENPEHKECGLHRR
>Mature_230_residues
MKSVPEVPSNASPQDILRLGLEAAEGPVKLACSFSLEDVAIIDLAKAAGLEIGVFALDTGRLNEETYEVADAISQRFGIS
IDWYFPRHEAVEKLEREKGLFSFRESLDNRHECCHIRKVEPLGRALQGLSGWITGMRREHNVTRIDLKSIELDQLNGGIL
KLNPLLDWNEEQLLAYVQQQRLPQNRLLKEGYRSIGCAPCTRAVQPGEDARAGRWWWENPEHKECGLHRR

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=211, Percent_Identity=28.436018957346, Blast_Score=90, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6325425, Length=126, Percent_Identity=43.6507936507937, Blast_Score=96, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011798
- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 26109; Mature: 26109

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSVPEVPSNASPQDILRLGLEAAEGPVKLACSFSLEDVAIIDLAKAAGLEIGVFALDTG
CCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEEECCC
RLNEETYEVADAISQRFGISIDWYFPRHEAVEKLEREKGLFSFRESLDNRHECCHIRKVE
CCCHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHEHHCCCC
PLGRALQGLSGWITGMRREHNVTRIDLKSIELDQLNGGILKLNPLLDWNEEQLLAYVQQQ
HHHHHHHHHHHHHHHHHHCCCCEEEEECCEEHHHCCCCEEEECCCCCCCHHHHHHHHHHH
RLPQNRLLKEGYRSIGCAPCTRAVQPGEDARAGRWWWENPEHKECGLHRR
CCCHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKSVPEVPSNASPQDILRLGLEAAEGPVKLACSFSLEDVAIIDLAKAAGLEIGVFALDTG
CCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEEECCC
RLNEETYEVADAISQRFGISIDWYFPRHEAVEKLEREKGLFSFRESLDNRHECCHIRKVE
CCCHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHEHHCCCC
PLGRALQGLSGWITGMRREHNVTRIDLKSIELDQLNGGILKLNPLLDWNEEQLLAYVQQQ
HHHHHHHHHHHHHHHHHHCCCCEEEEECCEEHHHCCCCEEEECCCCCCCHHHHHHHHHHH
RLPQNRLLKEGYRSIGCAPCTRAVQPGEDARAGRWWWENPEHKECGLHRR
CCCHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]