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The map label for this gene is cysN-1 [H]

Identifier: 197118733

GI number: 197118733

Start: 2728964

End: 2730619

Strand: Reverse

Name: cysN-1 [H]

Synonym: Gbem_2352

Alternate gene names: 197118733

Gene position: 2730619-2728964 (Counterclockwise)

Preceding gene: 197118734

Following gene: 308535299

Centisome position: 59.17

GC content: 63.22

Gene sequence:

>1656_bases
ATGGCACATCAATCGGAACTGATCGAGAAGGACATCCTCGCCTACCTGAAGAGTCAGGAGGAGAAATCCCTGCTCCGTTT
CATCACCTGCGGCAGCGTGGATGACGGCAAAAGCACCCTGATCGGGCGTCTCTTGTGGGACTCGAAGATGGTGTTCGAGG
ACCAGCTGGCAGCGCTTGAGGCGGACAGCAAGAAGGTGGGGACCCAGGGGGGCGCCATCGACTATGCCCTCCTTCTGGAC
GGGCTGCAGGCCGAGCGGGAGCAGGGGATAACCATCGACGTCGCCTATCGCTTCTTCTCCACCGACCGCCGCAAGTTCAT
CGTCGCCGACACCCCGGGCCACGAGCAGTACACCCGCAACATGGTAACCGGCGCCTCCACCGCCAAGGTGGCGGTGATCC
TGGTGGACGCCCGCAAGGGGCTTTTGACCCAGACCCGGCGCCACAGCTACCTGGTGTCGCTGGTGGGGATCCGACACATC
GTCTTGGCCATCAACAAGATGGACCTGATCGATTTCGACGCCGAGAAGTTCGCCGCCATCGAGAAGGATTACCGGGAATT
CGCCGCTCCGCTCGGCTTTAGCTCCATCACCGCACTCCCGATCTCGGCTTTGAACGGCGACAACATCATCGAGAAGAGCG
CCAACACCCCCTGGTACCAGGGGCCGCCGCTGCTGCACTTCCTGGAGACCGTGCAGGTCGAGGACGATCGCGCCGATCAG
GCCTTCCGGCTGCCGGTGCAGTGGGTGAACCGCCCCAACCTCGATTTCCGCGGGTTCTGCGGCACCGTCGCCTCCGGCGT
CATACGTCCCGGCGACGAGATCCGGGTCGCTTCATCGGGGCTGGTCAGCAAGGTCTCCAGGATCGTCACCATGAACGGCG
ACCTGAAAGAGGCCGTCGCCGGCCAGGCCGTGACGCTCACTTTGGAAGACGAGATCGACATCAGCCGCGGGGATATGCTG
ACCCGCGCCGAAGCGCCGCCGCTTTACACCAGGCATCCCGAGGCGCAGCTCGTCTGGCTTCACGACGAGCCGCTGCAGCC
CGGGCAGCTGTACCTGGTTAAAACTGCCACCGGTGTGACTCCCGGCAGGGTGACCGCGGTCCACTACGCGACCGACGTCA
ACACCTTGGAGCAGAAGCAGGTGGCGACGCTGGGGCTAAACGAGATCGGCCTGGCGCGGCTGGAACTCGACCGCCCGGTC
TCCTTTGACCCGTACCGCGAGAACAGGGATATCGGCAGCTTCATCCTTATTGATCGTTTCACCAACGCCACGGTCGCCGC
CGGCATGGTGGTCGAGGCTCTCCCGCAGGACGCCGCCGAGTTGACCGAGGGCGGGGCCGGAACCGGAAGCTCATGGGTCC
GGCGCATCAGCCTCGGCGAGTTGGCAGCTACCAATCTGAACCTTGTCGACCTGAGAGAGGAGAAGGGCGCTTTCGTAATC
GACGTACCGCGGGCGACCCTGGAGCACCTTGAGAAAGGGAACCGGCTCCTTTTCAGGCTGCGCGATCTGGGGCAGTTGGA
GCCGGTGGCGTTCGTAGCCTACGAGAACTGCCTTGCCTTCGAGTTCGACCGGACGCCTGAAGGGATAAGCGTGCTGCTTT
TCAAGAGGAGCAACAACCCCCAAAAGAACTGCGGGGATGACGGGATAGGGATCTAG

Upstream 100 bases:

>100_bases
AGGAAATGCTCCTCACCCGCACCTCCGAGCGCCAGGGGCGCCTGATCGACCACGACCAGGCCGGCTCCATGGAGAAGAAG
AAACAGGAGGGGTACTTCTA

Downstream 100 bases:

>100_bases
GGAGAAACCGGGACTGGGGGCTGGGGGCTAGGGGCTAGGGGCTAGGGGCTGGCGGCTTTCAGCAAAACTGACTTGACTGT
TTCATCTTGCCTTGTCCTCT

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MAHQSELIEKDILAYLKSQEEKSLLRFITCGSVDDGKSTLIGRLLWDSKMVFEDQLAALEADSKKVGTQGGAIDYALLLD
GLQAEREQGITIDVAYRFFSTDRRKFIVADTPGHEQYTRNMVTGASTAKVAVILVDARKGLLTQTRRHSYLVSLVGIRHI
VLAINKMDLIDFDAEKFAAIEKDYREFAAPLGFSSITALPISALNGDNIIEKSANTPWYQGPPLLHFLETVQVEDDRADQ
AFRLPVQWVNRPNLDFRGFCGTVASGVIRPGDEIRVASSGLVSKVSRIVTMNGDLKEAVAGQAVTLTLEDEIDISRGDML
TRAEAPPLYTRHPEAQLVWLHDEPLQPGQLYLVKTATGVTPGRVTAVHYATDVNTLEQKQVATLGLNEIGLARLELDRPV
SFDPYRENRDIGSFILIDRFTNATVAAGMVVEALPQDAAELTEGGAGTGSSWVRRISLGELAATNLNLVDLREEKGAFVI
DVPRATLEHLEKGNRLLFRLRDLGQLEPVAFVAYENCLAFEFDRTPEGISVLLFKRSNNPQKNCGDDGIGI

Sequences:

>Translated_551_residues
MAHQSELIEKDILAYLKSQEEKSLLRFITCGSVDDGKSTLIGRLLWDSKMVFEDQLAALEADSKKVGTQGGAIDYALLLD
GLQAEREQGITIDVAYRFFSTDRRKFIVADTPGHEQYTRNMVTGASTAKVAVILVDARKGLLTQTRRHSYLVSLVGIRHI
VLAINKMDLIDFDAEKFAAIEKDYREFAAPLGFSSITALPISALNGDNIIEKSANTPWYQGPPLLHFLETVQVEDDRADQ
AFRLPVQWVNRPNLDFRGFCGTVASGVIRPGDEIRVASSGLVSKVSRIVTMNGDLKEAVAGQAVTLTLEDEIDISRGDML
TRAEAPPLYTRHPEAQLVWLHDEPLQPGQLYLVKTATGVTPGRVTAVHYATDVNTLEQKQVATLGLNEIGLARLELDRPV
SFDPYRENRDIGSFILIDRFTNATVAAGMVVEALPQDAAELTEGGAGTGSSWVRRISLGELAATNLNLVDLREEKGAFVI
DVPRATLEHLEKGNRLLFRLRDLGQLEPVAFVAYENCLAFEFDRTPEGISVLLFKRSNNPQKNCGDDGIGI
>Mature_550_residues
AHQSELIEKDILAYLKSQEEKSLLRFITCGSVDDGKSTLIGRLLWDSKMVFEDQLAALEADSKKVGTQGGAIDYALLLDG
LQAEREQGITIDVAYRFFSTDRRKFIVADTPGHEQYTRNMVTGASTAKVAVILVDARKGLLTQTRRHSYLVSLVGIRHIV
LAINKMDLIDFDAEKFAAIEKDYREFAAPLGFSSITALPISALNGDNIIEKSANTPWYQGPPLLHFLETVQVEDDRADQA
FRLPVQWVNRPNLDFRGFCGTVASGVIRPGDEIRVASSGLVSKVSRIVTMNGDLKEAVAGQAVTLTLEDEIDISRGDMLT
RAEAPPLYTRHPEAQLVWLHDEPLQPGQLYLVKTATGVTPGRVTAVHYATDVNTLEQKQVATLGLNEIGLARLELDRPVS
FDPYRENRDIGSFILIDRFTNATVAAGMVVEALPQDAAELTEGGAGTGSSWVRRISLGELAATNLNLVDLREEKGAFVID
VPRATLEHLEKGNRLLFRLRDLGQLEPVAFVAYENCLAFEFDRTPEGISVLLFKRSNNPQKNCGDDGIGI

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=444, Percent_Identity=31.7567567567568, Blast_Score=189, Evalue=8e-48,
Organism=Homo sapiens, GI5729864, Length=444, Percent_Identity=31.7567567567568, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI4503475, Length=466, Percent_Identity=26.8240343347639, Blast_Score=166, Evalue=6e-41,
Organism=Homo sapiens, GI4503471, Length=357, Percent_Identity=29.4117647058824, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI46094014, Length=454, Percent_Identity=26.6519823788546, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI194018522, Length=433, Percent_Identity=26.3279445727483, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI194097354, Length=433, Percent_Identity=26.3279445727483, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI194018520, Length=433, Percent_Identity=26.3279445727483, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI34147630, Length=311, Percent_Identity=28.2958199356913, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI157426893, Length=346, Percent_Identity=25.7225433526012, Blast_Score=66, Evalue=9e-11,
Organism=Escherichia coli, GI1789108, Length=439, Percent_Identity=54.8974943052392, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI1789737, Length=344, Percent_Identity=27.906976744186, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790412, Length=344, Percent_Identity=27.906976744186, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI2367247, Length=259, Percent_Identity=25.4826254826255, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17552884, Length=466, Percent_Identity=27.8969957081545, Blast_Score=174, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI17569207, Length=466, Percent_Identity=27.8969957081545, Blast_Score=174, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI32566629, Length=439, Percent_Identity=28.0182232346241, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI115532067, Length=314, Percent_Identity=29.6178343949045, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI115532065, Length=314, Percent_Identity=29.6178343949045, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI32566303, Length=459, Percent_Identity=26.797385620915, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25141371, Length=319, Percent_Identity=26.6457680250784, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=34.8993288590604, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17556456, Length=278, Percent_Identity=27.3381294964029, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6325337, Length=447, Percent_Identity=28.6353467561521, Blast_Score=180, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6319594, Length=447, Percent_Identity=28.6353467561521, Blast_Score=180, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6322937, Length=463, Percent_Identity=28.7257019438445, Blast_Score=167, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6320377, Length=434, Percent_Identity=26.9585253456221, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324761, Length=284, Percent_Identity=30.9859154929577, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI45550900, Length=433, Percent_Identity=31.6397228637413, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24652838, Length=458, Percent_Identity=27.0742358078603, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI17137572, Length=458, Percent_Identity=27.0742358078603, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI45553807, Length=449, Percent_Identity=26.7260579064588, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI45553816, Length=449, Percent_Identity=26.7260579064588, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24651721, Length=449, Percent_Identity=26.7260579064588, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI17864154, Length=449, Percent_Identity=26.7260579064588, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI17137380, Length=437, Percent_Identity=26.5446224256293, Blast_Score=150, Evalue=3e-36,
Organism=Drosophila melanogaster, GI281363316, Length=304, Percent_Identity=29.2763157894737, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI17864358, Length=304, Percent_Identity=29.2763157894737, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI19921738, Length=429, Percent_Identity=27.039627039627, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 60695; Mature: 60564

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHQSELIEKDILAYLKSQEEKSLLRFITCGSVDDGKSTLIGRLLWDSKMVFEDQLAALE
CCCHHHHHHHHHHHHHHCCHHHHHHHEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHC
ADSKKVGTQGGAIDYALLLDGLQAEREQGITIDVAYRFFSTDRRKFIVADTPGHEQYTRN
CCCHHCCCCCCCEEHHHHHHCHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHHHHH
MVTGASTAKVAVILVDARKGLLTQTRRHSYLVSLVGIRHIVLAINKMDLIDFDAEKFAAI
HHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHEECCCEEEECCHHHHHHH
EKDYREFAAPLGFSSITALPISALNGDNIIEKSANTPWYQGPPLLHFLETVQVEDDRADQ
HHHHHHHHCCCCCCCCCCCEEEECCCCCHHHCCCCCCCCCCCHHHHHHHHHEECCCCCCC
AFRLPVQWVNRPNLDFRGFCGTVASGVIRPGDEIRVASSGLVSKVSRIVTMNGDLKEAVA
EEECCHHHCCCCCCCCCCCHHHHHCCCCCCCCCEEEECHHHHHHHHHEEEECCCHHHHHC
GQAVTLTLEDEIDISRGDMLTRAEAPPLYTRHPEAQLVWLHDEPLQPGQLYLVKTATGVT
CCEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCC
PGRVTAVHYATDVNTLEQKQVATLGLNEIGLARLELDRPVSFDPYRENRDIGSFILIDRF
CCCEEEEEEECCCCHHHHHHHHHCCCCHHCEEEEEECCCCCCCCCCCCCCCCCEEEEEEC
TNATVAAGMVVEALPQDAAELTEGGAGTGSSWVRRISLGELAATNLNLVDLREEKGAFVI
CCCHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHCHHHHCCCEEEEECCCCCCEEE
DVPRATLEHLEKGNRLLFRLRDLGQLEPVAFVAYENCLAFEFDRTPEGISVLLFKRSNNP
ECCHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCEEEEEECCCCCCEEEEEEECCCCC
QKNCGDDGIGI
CCCCCCCCCCC
>Mature Secondary Structure 
AHQSELIEKDILAYLKSQEEKSLLRFITCGSVDDGKSTLIGRLLWDSKMVFEDQLAALE
CCHHHHHHHHHHHHHHCCHHHHHHHEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHC
ADSKKVGTQGGAIDYALLLDGLQAEREQGITIDVAYRFFSTDRRKFIVADTPGHEQYTRN
CCCHHCCCCCCCEEHHHHHHCHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHHHHH
MVTGASTAKVAVILVDARKGLLTQTRRHSYLVSLVGIRHIVLAINKMDLIDFDAEKFAAI
HHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHEECCCEEEECCHHHHHHH
EKDYREFAAPLGFSSITALPISALNGDNIIEKSANTPWYQGPPLLHFLETVQVEDDRADQ
HHHHHHHHCCCCCCCCCCCEEEECCCCCHHHCCCCCCCCCCCHHHHHHHHHEECCCCCCC
AFRLPVQWVNRPNLDFRGFCGTVASGVIRPGDEIRVASSGLVSKVSRIVTMNGDLKEAVA
EEECCHHHCCCCCCCCCCCHHHHHCCCCCCCCCEEEECHHHHHHHHHEEEECCCHHHHHC
GQAVTLTLEDEIDISRGDMLTRAEAPPLYTRHPEAQLVWLHDEPLQPGQLYLVKTATGVT
CCEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCC
PGRVTAVHYATDVNTLEQKQVATLGLNEIGLARLELDRPVSFDPYRENRDIGSFILIDRF
CCCEEEEEEECCCCHHHHHHHHHCCCCHHCEEEEEECCCCCCCCCCCCCCCCCEEEEEEC
TNATVAAGMVVEALPQDAAELTEGGAGTGSSWVRRISLGELAATNLNLVDLREEKGAFVI
CCCHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHCHHHHCCCEEEEECCCCCCEEE
DVPRATLEHLEKGNRLLFRLRDLGQLEPVAFVAYENCLAFEFDRTPEGISVLLFKRSNNP
ECCHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCEEEEEECCCCCCEEEEEEECCCCC
QKNCGDDGIGI
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA