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The map label for this gene is birA [H]

Identifier: 197118701

GI number: 197118701

Start: 2693502

End: 2694485

Strand: Reverse

Name: birA [H]

Synonym: Gbem_2320

Alternate gene names: 197118701

Gene position: 2694485-2693502 (Counterclockwise)

Preceding gene: 197118702

Following gene: 197118700

Centisome position: 58.38

GC content: 63.21

Gene sequence:

>984_bases
TTGAAAGAATCCGGAGTAGACGGGAAAATCCTGGAACTGTTCCGCTCCGGAGACGGTGTGGTATCCGGCGCCGCGCTCAG
CAAGGCGCTGGGGGTTTCGCGAACCGCAGTCTGGAAGCACGTCAAGGGGTTAAGGGGCAAAGGGTACCAGATCGAAGCTG
TCCCCTCCAAGGGGTACCGGCTGCTCTCCTCCCCCGAAATTCTCACCAATCTCGACATCACCGCCGGCCTCGGCACCAAG
AGGGTCGGCTCCTCGGTCATCTGCCTCAAGGAGACCGATTCCACCAACTCCGTCGCCTTCAAGATGGCGGAGGAGGGGGC
TCCCGAAGGGACCGTGGTCATCGCCGACACCCAGTCGGCCGGCAAGGGGCGGCTAGGTCGGATCTGGCTCAGCCCCCCCG
GAGTGAACCTTTACTGCTCCGTGGTACTGCGCCCGGCGATAGCCCCGGTGGCTGCCTGCCAGCTCACCTTCCTCTCGGTG
GTCGCCGTGGCCCGCGCCGTCGAGCGCTGCACCAACCTTAAGCCGCAGATCAAGTGGCCCAACGACATCCTGATCAACGG
GAAAAAGGTTGCCGGGCTTCTGAACGAGATGAATGCTGAGACCGAGAAGGTGAACTTCGTGGTTCTTGGCATCGGGGTCA
ACCTTAACCTCCGCATGGCCGTCATCAGCGAAGGACTTTTGCGCCACCCCGCGACCTCGCTCCTGGAGGAGGGGGGAGAG
GAGGTGGACCGGCTGAGGTTCACCCGCGCCCTTTTAGAGGAACTGGACCAGCTGTACGACAATTTTCTGGTGCAAGGGGA
CGGACCGGTCCGGGCCGAGTGGCTGGCGCGTTCCGCTATCAAGGGGCGCAGCGTAAGGGTGAGCCAGCAAAACCGCGAAT
TCAGCGGCGTGGTGCAGGGTGTGGATTCCTTCGGGGCGCTCCTGGTGCTCCTGGGCGACGGCACCCTGGAAACGGTGCTG
TCCGGGGACGTCGCGTTCATCTAA

Upstream 100 bases:

>100_bases
CCATCAAATCCATCGCCCAGACCGGGGTGGACATCATATCGGTCGGGGCCTTGACCCACTCGGTCAGGGCCACCGACATC
TCCATGCTGATGGAGGAAGT

Downstream 100 bases:

>100_bases
AAACGCCTCACGCAAAGCCGCGAAGGCGCAAAGAAAGACGAAACTAAATGAGTAGGTCTCGAAGGTTTGCTTTGCGCCTT
TGCGGCTTTGCGTGAGGAGG

Product: bifunctional biotin operon repressor/biotin--acetyl-CoA carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKESGVDGKILELFRSGDGVVSGAALSKALGVSRTAVWKHVKGLRGKGYQIEAVPSKGYRLLSSPEILTNLDITAGLGTK
RVGSSVICLKETDSTNSVAFKMAEEGAPEGTVVIADTQSAGKGRLGRIWLSPPGVNLYCSVVLRPAIAPVAACQLTFLSV
VAVARAVERCTNLKPQIKWPNDILINGKKVAGLLNEMNAETEKVNFVVLGIGVNLNLRMAVISEGLLRHPATSLLEEGGE
EVDRLRFTRALLEELDQLYDNFLVQGDGPVRAEWLARSAIKGRSVRVSQQNREFSGVVQGVDSFGALLVLLGDGTLETVL
SGDVAFI

Sequences:

>Translated_327_residues
MKESGVDGKILELFRSGDGVVSGAALSKALGVSRTAVWKHVKGLRGKGYQIEAVPSKGYRLLSSPEILTNLDITAGLGTK
RVGSSVICLKETDSTNSVAFKMAEEGAPEGTVVIADTQSAGKGRLGRIWLSPPGVNLYCSVVLRPAIAPVAACQLTFLSV
VAVARAVERCTNLKPQIKWPNDILINGKKVAGLLNEMNAETEKVNFVVLGIGVNLNLRMAVISEGLLRHPATSLLEEGGE
EVDRLRFTRALLEELDQLYDNFLVQGDGPVRAEWLARSAIKGRSVRVSQQNREFSGVVQGVDSFGALLVLLGDGTLETVL
SGDVAFI
>Mature_327_residues
MKESGVDGKILELFRSGDGVVSGAALSKALGVSRTAVWKHVKGLRGKGYQIEAVPSKGYRLLSSPEILTNLDITAGLGTK
RVGSSVICLKETDSTNSVAFKMAEEGAPEGTVVIADTQSAGKGRLGRIWLSPPGVNLYCSVVLRPAIAPVAACQLTFLSV
VAVARAVERCTNLKPQIKWPNDILINGKKVAGLLNEMNAETEKVNFVVLGIGVNLNLRMAVISEGLLRHPATSLLEEGGE
EVDRLRFTRALLEELDQLYDNFLVQGDGPVRAEWLARSAIKGRSVRVSQQNREFSGVVQGVDSFGALLVLLGDGTLETVL
SGDVAFI

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=335, Percent_Identity=32.2388059701493, Blast_Score=155, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24644246, Length=281, Percent_Identity=26.6903914590747, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 34895; Mature: 34895

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKESGVDGKILELFRSGDGVVSGAALSKALGVSRTAVWKHVKGLRGKGYQIEAVPSKGYR
CCCCCCCHHHHHHHHCCCCEEEHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCCEE
LLSSPEILTNLDITAGLGTKRVGSSVICLKETDSTNSVAFKMAEEGAPEGTVVIADTQSA
EECCCCHHEECCEECCCCCHHCCCEEEEEEECCCCCCEEEEEHHCCCCCCEEEEEECCCC
GKGRLGRIWLSPPGVNLYCSVVLRPAIAPVAACQLTFLSVVAVARAVERCTNLKPQIKWP
CCCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECC
NDILINGKKVAGLLNEMNAETEKVNFVVLGIGVNLNLRMAVISEGLLRHPATSLLEEGGE
CCEEECCHHHHHHHHHCCCCCEEEEEEEEEECCCCEEEHHHHHHHHHHCHHHHHHHCCCH
EVDRLRFTRALLEELDQLYDNFLVQGDGPVRAEWLARSAIKGRSVRVSQQNREFSGVVQG
HHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHH
VDSFGALLVLLGDGTLETVLSGDVAFI
HHHHCEEEEEECCCCHHHHHCCCEECC
>Mature Secondary Structure
MKESGVDGKILELFRSGDGVVSGAALSKALGVSRTAVWKHVKGLRGKGYQIEAVPSKGYR
CCCCCCCHHHHHHHHCCCCEEEHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCCEE
LLSSPEILTNLDITAGLGTKRVGSSVICLKETDSTNSVAFKMAEEGAPEGTVVIADTQSA
EECCCCHHEECCEECCCCCHHCCCEEEEEEECCCCCCEEEEEHHCCCCCCEEEEEECCCC
GKGRLGRIWLSPPGVNLYCSVVLRPAIAPVAACQLTFLSVVAVARAVERCTNLKPQIKWP
CCCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECC
NDILINGKKVAGLLNEMNAETEKVNFVVLGIGVNLNLRMAVISEGLLRHPATSLLEEGGE
CCEEECCHHHHHHHHHCCCCCEEEEEEEEEECCCCEEEHHHHHHHHHHCHHHHHHHCCCH
EVDRLRFTRALLEELDQLYDNFLVQGDGPVRAEWLARSAIKGRSVRVSQQNREFSGVVQG
HHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHH
VDSFGALLVLLGDGTLETVLSGDVAFI
HHHHCEEEEEECCCCHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]