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The map label for this gene is genX [H]

Identifier: 197118436

GI number: 197118436

Start: 2368509

End: 2369417

Strand: Reverse

Name: genX [H]

Synonym: Gbem_2054

Alternate gene names: 197118436

Gene position: 2369417-2368509 (Counterclockwise)

Preceding gene: 197118437

Following gene: 197118435

Centisome position: 51.34

GC content: 61.72

Gene sequence:

>909_bases
ATGGGAAGCTGGCCCCTGGCCAGGCGCCGCCAGGCGCTGGTCGAGCGGGGGGCGATCTTCAGCAGGATAAGAGAGTTTTT
TCAAGAAAAGGGGTATCTCGAAGTCGAGACCCCTTTTCGCATACCTGCCCCCGCCCCTGAAGCCCAGATCGACGCTATCC
CCGCCTCGGGATGGTTCCTGCAGACCTCCCCGGAACTCTGCATGAAGCGGATGCTTGCGGCAGGCTACCAGCGCATCTTC
CAGATCTGCCGCTGCTGGCGTGACGGCGAGCGCGGCGCGAGGCACCTGAGCGAGTTCACCATGCTGGAGTGGTACCGTTC
CGAAGCCGACTACCTGGTGCTGATGGAGGAAACGGAAGCGCTCGTCCAGCACGCCGCCGCAACCGGCAGCATAAGCTACC
GCGGACAGCAGATAGAACTTAGCGGCAAGTGGGAGAGGATCACCGTCGCCAACGCCTTCCTGCGTTACGCCGGGACCTCC
ATGTGGCCGGCGCTTGCCGAGGGCACCTTCGACGAAATCATGGTCGAGCTCATCGAGCCCAATCTCGGCCTTGCCCGCCC
CACCTTCATCTACGATTATCCGGCCAGTTGTTCCGCCCTCGCCCGACTCAAGCCTTCCGACCCAACAGTTGCCGAACGTT
TCGAGCTCTACATCGGCGGACTCGAAATCGCCAACGCATTTTCCGAACTGATCGACCCGGTCGAACAGCGGGCGAGATTC
GTTGCCGAAGCCGCCGAACGTGCGGCGCAGGGAAAGACCGGTTACCCCATGCCGGAGAAGTTCCTGTCCGCCCTAGACGA
GATGCCGGAAGCCGCAGGAATAGCCTTGGGACTCGACCGTCTGGTCATGGTGCTGCTCGATGCCGAAATTATCGACGAGG
TAGTCGCCTTCACGACGGAAGAGCTTTAA

Upstream 100 bases:

>100_bases
CGGCTACGTGCTCCGCGTTCCCCCCTTCATCGAAGAGGGAGAACTGATCACCATCGACACCCGCACCGGCGAATACTCCA
CCAGGGTAAAGGGGTAACGC

Downstream 100 bases:

>100_bases
CCCCCTCTACTAACCCTCATCCCCACCCCCCACTCCCCGGTCATGAGATGGGCGCGTTTCAATTTGTCATGACCGCGTGC
CAATTTGTCATGGACGCGTT

Product: lysine adenylyltransferase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MGSWPLARRRQALVERGAIFSRIREFFQEKGYLEVETPFRIPAPAPEAQIDAIPASGWFLQTSPELCMKRMLAAGYQRIF
QICRCWRDGERGARHLSEFTMLEWYRSEADYLVLMEETEALVQHAAATGSISYRGQQIELSGKWERITVANAFLRYAGTS
MWPALAEGTFDEIMVELIEPNLGLARPTFIYDYPASCSALARLKPSDPTVAERFELYIGGLEIANAFSELIDPVEQRARF
VAEAAERAAQGKTGYPMPEKFLSALDEMPEAAGIALGLDRLVMVLLDAEIIDEVVAFTTEEL

Sequences:

>Translated_302_residues
MGSWPLARRRQALVERGAIFSRIREFFQEKGYLEVETPFRIPAPAPEAQIDAIPASGWFLQTSPELCMKRMLAAGYQRIF
QICRCWRDGERGARHLSEFTMLEWYRSEADYLVLMEETEALVQHAAATGSISYRGQQIELSGKWERITVANAFLRYAGTS
MWPALAEGTFDEIMVELIEPNLGLARPTFIYDYPASCSALARLKPSDPTVAERFELYIGGLEIANAFSELIDPVEQRARF
VAEAAERAAQGKTGYPMPEKFLSALDEMPEAAGIALGLDRLVMVLLDAEIIDEVVAFTTEEL
>Mature_301_residues
GSWPLARRRQALVERGAIFSRIREFFQEKGYLEVETPFRIPAPAPEAQIDAIPASGWFLQTSPELCMKRMLAAGYQRIFQ
ICRCWRDGERGARHLSEFTMLEWYRSEADYLVLMEETEALVQHAAATGSISYRGQQIELSGKWERITVANAFLRYAGTSM
WPALAEGTFDEIMVELIEPNLGLARPTFIYDYPASCSALARLKPSDPTVAERFELYIGGLEIANAFSELIDPVEQRARFV
AEAAERAAQGKTGYPMPEKFLSALDEMPEAAGIALGLDRLVMVLLDAEIIDEVVAFTTEEL

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=332, Percent_Identity=30.1204819277108, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI194272210, Length=332, Percent_Identity=30.1204819277108, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI87082379, Length=321, Percent_Identity=38.3177570093458, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1790571, Length=322, Percent_Identity=32.9192546583851, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1789256, Length=318, Percent_Identity=32.7044025157233, Blast_Score=161, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI71994340, Length=337, Percent_Identity=27.299703264095, Blast_Score=120, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17535925, Length=337, Percent_Identity=27.299703264095, Blast_Score=120, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17535927, Length=337, Percent_Identity=27.299703264095, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320242, Length=336, Percent_Identity=29.1666666666667, Blast_Score=125, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24640849, Length=332, Percent_Identity=28.9156626506024, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24640851, Length=332, Percent_Identity=28.9156626506024, Blast_Score=131, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 33931; Mature: 33800

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00501 SPASE_I_1 ; PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSWPLARRRQALVERGAIFSRIREFFQEKGYLEVETPFRIPAPAPEAQIDAIPASGWFL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEECCCCCCCCCCCCCCCCCCEE
QTSPELCMKRMLAAGYQRIFQICRCWRDGERGARHLSEFTMLEWYRSEADYLVLMEETEA
ECCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHH
LVQHAAATGSISYRGQQIELSGKWERITVANAFLRYAGTSMWPALAEGTFDEIMVELIEP
HHHHHHHCCCEEECCCEEEECCCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHCC
NLGLARPTFIYDYPASCSALARLKPSDPTVAERFELYIGGLEIANAFSELIDPVEQRARF
CCCCCCCCEEEECCCCHHHHHHCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
VAEAAERAAQGKTGYPMPEKFLSALDEMPEAAGIALGLDRLVMVLLDAEIIDEVVAFTTE
HHHHHHHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EL
CC
>Mature Secondary Structure 
GSWPLARRRQALVERGAIFSRIREFFQEKGYLEVETPFRIPAPAPEAQIDAIPASGWFL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEECCCCCCCCCCCCCCCCCCEE
QTSPELCMKRMLAAGYQRIFQICRCWRDGERGARHLSEFTMLEWYRSEADYLVLMEETEA
ECCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHH
LVQHAAATGSISYRGQQIELSGKWERITVANAFLRYAGTSMWPALAEGTFDEIMVELIEP
HHHHHHHCCCEEECCCEEEECCCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHCC
NLGLARPTFIYDYPASCSALARLKPSDPTVAERFELYIGGLEIANAFSELIDPVEQRARF
CCCCCCCCEEEECCCCHHHHHHCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
VAEAAERAAQGKTGYPMPEKFLSALDEMPEAAGIALGLDRLVMVLLDAEIIDEVVAFTTE
HHHHHHHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EL
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA