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The map label for this gene is bioB

Identifier: 197118435

GI number: 197118435

Start: 2366879

End: 2367865

Strand: Reverse

Name: bioB

Synonym: Gbem_2053

Alternate gene names: 197118435

Gene position: 2367865-2366879 (Counterclockwise)

Preceding gene: 197118436

Following gene: 197118434

Centisome position: 51.31

GC content: 61.7

Gene sequence:

>987_bases
ATGGAAAAGATGATCAATGACATAGCCCATAGGATCATAGCAGGTGGTTCGATCACCGAAGCAGAGGCCATCCAACTCAC
GCAAGTGCAGGGAACCGAGGTCTACGACCTCTTCCGCGCCGCCACTCGCGTGAAGGAGCATTTCGTCGGCAACGAGGTGC
ATCTCTGTTCCATAATCAACGCCAAGTCCGGCCGCTGCGCCGAGAACTGCGCCTTCTGCGCCCAGTCGGCGCACCACAAA
ACGGACGCACCGGTGTACCCGCTGGTCCAAGAGGAAGAGATGCTGGCTTCGGCACGCATGGCCGAGACCAACGGCTCTGC
CTGCTTCGGCATCATCACCAGCGGCACCACCGTCAACGGCCCCGAACTGGAGCAGATCCTCACGGCGCTGCGCCGCATCA
GGAAGGAAACCACCATCCTCCCCTCCTGCTCCCTCGGCATCATCGACGAGGAGACTGCGAGAAAGCTCAAGGAAGCCGGG
ATGGACACCTACCACCACAACCTTGAGACCGCTGCGAGCTTCTTCCCGCAGATCTGCACCACGCACGACTACCAGGACGA
CGTGAACACGGTGCGCGCGGTAAAGAAGGCCGGGGTCAAGGTCTGCTGCGGCGGCATCTTCGGGCTTGGCGAAAGCGCAG
CCCAGCGTGTGGAGATGGCGCTCACCCTCAAGGATCTCGACGTCGACTCCGTGCCGATGAACTTCCTGAACCCAATCGAG
GGGACGAGGCTTGAGGGGGCCGCGAACATCACCGCCCAGGAGTGCCTGAAGACCATCGCCATCTACCGGCTGATCCTCCC
CGGCAAGCGCATCACCGTCTGCGGTGGCAGGGAAAAGAACCTGCGCGACCTGCAGTCCTGGATCTTCTTCGCCGGCGCGA
ACGGCACCATGATCGGCAACTACCTGACCACGCTGGGGAGAAACGTCGACACCGACCTCACCATGTTCAGCGACCTGGGG
CTTAAGACCGTCATGTGCGCCCACTGA

Upstream 100 bases:

>100_bases
CCACCGAATCATACTTCCAACCTGCGCCTCGTCAACCTGCTACGCTGATTTGGTTGACAACCTCGTCACCACATGTTAAA
TCAGCGCCTGGAGGATTTAC

Downstream 100 bases:

>100_bases
GCCGCCCTCAAATACCGCCACGTAAGGAAAGATAACCATGGCTGCAAAAAGCATTTTCATAACCGGCACCGACACCGGGG
TCGGCAAGACCATCGCATCG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MEKMINDIAHRIIAGGSITEAEAIQLTQVQGTEVYDLFRAATRVKEHFVGNEVHLCSIINAKSGRCAENCAFCAQSAHHK
TDAPVYPLVQEEEMLASARMAETNGSACFGIITSGTTVNGPELEQILTALRRIRKETTILPSCSLGIIDEETARKLKEAG
MDTYHHNLETAASFFPQICTTHDYQDDVNTVRAVKKAGVKVCCGGIFGLGESAAQRVEMALTLKDLDVDSVPMNFLNPIE
GTRLEGAANITAQECLKTIAIYRLILPGKRITVCGGREKNLRDLQSWIFFAGANGTMIGNYLTTLGRNVDTDLTMFSDLG
LKTVMCAH

Sequences:

>Translated_328_residues
MEKMINDIAHRIIAGGSITEAEAIQLTQVQGTEVYDLFRAATRVKEHFVGNEVHLCSIINAKSGRCAENCAFCAQSAHHK
TDAPVYPLVQEEEMLASARMAETNGSACFGIITSGTTVNGPELEQILTALRRIRKETTILPSCSLGIIDEETARKLKEAG
MDTYHHNLETAASFFPQICTTHDYQDDVNTVRAVKKAGVKVCCGGIFGLGESAAQRVEMALTLKDLDVDSVPMNFLNPIE
GTRLEGAANITAQECLKTIAIYRLILPGKRITVCGGREKNLRDLQSWIFFAGANGTMIGNYLTTLGRNVDTDLTMFSDLG
LKTVMCAH
>Mature_328_residues
MEKMINDIAHRIIAGGSITEAEAIQLTQVQGTEVYDLFRAATRVKEHFVGNEVHLCSIINAKSGRCAENCAFCAQSAHHK
TDAPVYPLVQEEEMLASARMAETNGSACFGIITSGTTVNGPELEQILTALRRIRKETTILPSCSLGIIDEETARKLKEAG
MDTYHHNLETAASFFPQICTTHDYQDDVNTVRAVKKAGVKVCCGGIFGLGESAAQRVEMALTLKDLDVDSVPMNFLNPIE
GTRLEGAANITAQECLKTIAIYRLILPGKRITVCGGREKNLRDLQSWIFFAGANGTMIGNYLTTLGRNVDTDLTMFSDLG
LKTVMCAH

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=304, Percent_Identity=34.8684210526316, Blast_Score=182, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321725, Length=316, Percent_Identity=31.0126582278481, Blast_Score=158, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_GEOBB (B5ECN1)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002138862.1
- ProteinModelPortal:   B5ECN1
- SMR:   B5ECN1
- GeneID:   6782047
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_2053
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK828421
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 35748; Mature: 35748

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKMINDIAHRIIAGGSITEAEAIQLTQVQGTEVYDLFRAATRVKEHFVGNEVHLCSIIN
CHHHHHHHHHHHHCCCCCCHHHHEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEC
AKSGRCAENCAFCAQSAHHKTDAPVYPLVQEEEMLASARMAETNGSACFGIITSGTTVNG
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
PELEQILTALRRIRKETTILPSCSLGIIDEETARKLKEAGMDTYHHNLETAASFFPQICT
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
THDYQDDVNTVRAVKKAGVKVCCGGIFGLGESAAQRVEMALTLKDLDVDSVPMNFLNPIE
CCCCHHHHHHHHHHHHCCCEEEECHHHCCCHHHHHHHHHHEEEECCCCCCCCHHHCCCCC
GTRLEGAANITAQECLKTIAIYRLILPGKRITVCGGREKNLRDLQSWIFFAGANGTMIGN
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHEEECCCCCCHHH
YLTTLGRNVDTDLTMFSDLGLKTVMCAH
HHHHHCCCCCCCHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MEKMINDIAHRIIAGGSITEAEAIQLTQVQGTEVYDLFRAATRVKEHFVGNEVHLCSIIN
CHHHHHHHHHHHHCCCCCCHHHHEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEC
AKSGRCAENCAFCAQSAHHKTDAPVYPLVQEEEMLASARMAETNGSACFGIITSGTTVNG
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
PELEQILTALRRIRKETTILPSCSLGIIDEETARKLKEAGMDTYHHNLETAASFFPQICT
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
THDYQDDVNTVRAVKKAGVKVCCGGIFGLGESAAQRVEMALTLKDLDVDSVPMNFLNPIE
CCCCHHHHHHHHHHHHCCCEEEECHHHCCCHHHHHHHHHHEEEECCCCCCCCHHHCCCCC
GTRLEGAANITAQECLKTIAIYRLILPGKRITVCGGREKNLRDLQSWIFFAGANGTMIGN
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHEEECCCCCCHHH
YLTTLGRNVDTDLTMFSDLGLKTVMCAH
HHHHHCCCCCCCHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA