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The map label for this gene is ntrC [H]

Identifier: 197118370

GI number: 197118370

Start: 2292149

End: 2293678

Strand: Direct

Name: ntrC [H]

Synonym: Gbem_1988

Alternate gene names: 197118370

Gene position: 2292149-2293678 (Clockwise)

Preceding gene: 197118369

Following gene: 197118371

Centisome position: 49.67

GC content: 63.14

Gene sequence:

>1530_bases
ATGACGGACTCATCTACTAGAATACTTATCATCGACGACGAGAGGGATGTCTGCACCTTTTTCAGCCGGCTTTTGTCGCG
CAAGGGGTACCAGGTGGTCACCGCCGCCAGCGAGCCCGAGGCGCTCTCCGCGTTGGAGGCGGCGCAGTTCAGCGTGGCGC
TCGTAGACCTGAAGCTCCCCGACACCGACGGCATCACCCTGCTGGAGATCATCAAGTCGCGGCAGCCCGCCTGCGAGGTC
ATCATCATGACCGGGTACTCCACCGTCAAGACGGCGGTCGCCGCCATGCAGCTCGGTGCCTACGAATACCTGGAAAAGCC
CTTCGACGACATCGACCAGATCGAGCAGTTGGTCGGCAGGGCCGCTGGTTCAGGCGCAAGCCTCCTGAGAGGGGACAACT
CCCACGACGAGTGGGCCGAAGTGGCCCAGGGGGTAGGTTTCAGGGTCGGGAGCTCTCCGTCCATGAAGAGGATGGTCTCA
CTCGCCTACAAGGTCGCCGGCAAGGACATCAGCGTCCTGATCCAGGGGAAGACCGGCACCGGGAAAGAGGTGCTGGCCCG
CTTCATCCACGCCGCCTCCTGCCGCGCCGGGCAGCCCTTCATCCCGGTCAACTGCGGCGCGCTACCCGAGAACCTCCTGG
AGGGCGAACTCTTCGGGCACGAAAAGGGCGCCTTCACCGGCGCCAACCAGACCCGCCGCGGCATCTTCGAGCTCGCCAAC
AACGGGACGCTCCTCTTGGACGAGATCGGGGACGCGACACCGCAGATCCAGGTCAAACTTTTGCGCGTCTTGGAAACCGG
CGAGTTCATGAGGGTAGGGGGGGAGCGCCCCATAAGGACCGACGTCAGGGTGATAGCCGCGACCAACGTGGACCTGGAGC
AGGCCATTGCCGAGAAGAGCTTCCGCGAGGACCTCTTTTACCGCCTGAACGTGGTGCGGCTGGAGATACCGTCTCTTAGC
GCCCGTTCCGAGGACATCCCGCTTTTGGCCGAGCATTTCGTGCACCAGATAAATCGCGAACTGAAGCTTGCCGCGGGAAC
CTTGCGCATGCTGCAGGGGTACGCCTGGCCTGGGAACATCCGCGAACTGGCTAACGTGATGAGGCGGGCCGTGGTGATCT
GCAACGGCGACACCATTCTTCCCGAGCACCTGGGTGGGACCTTTCTCGCGGGACCCACGGCACCGGAGCGGGGGAGTGCG
GCGAAAACAATCCAGCAACCGGACCGCAGCAGGCTGCAGGCTACACTCCTGGAGCATTGTGACAGCGCTGAAGAGCTGGA
GCGGCTGGATGCCGAAGAGCTGAACCGGCTTTTGGATTCGTTGCGCCAGGCGCAGTCGAACCTCCTCGGCGTTATGCGCA
AAAAGAAGATCGTTCCGGCCCTGCACGCGCTGAAGGATTCCGAAGCCGAGACCATCAAGGAAGCGCTGGCGCAGTACGAC
GGCAACATCACCGAGGCCGCGAGGGCGCTGGGGATAGCCCGCAACACCCTCTACAGAAAGATCAAGGAGCTGGGGCTGCC
GGGAAGGTAG

Upstream 100 bases:

>100_bases
CGGCCGCATCGAGGTCGACACCGAACTTGGGCAGGGGAGCTGTTTCAGGTTGATTCTTCCCGTCGACCAGCAGTAAGCAG
ACATATATATTGGAGCAACC

Downstream 100 bases:

>100_bases
CCGCTCCAGGGGGGCTCTTCCGGGATTGCGGTGATACTAGCCCCCCTCCCGCAAGGGGAGGGGGGCTAAGAGCGGCTTTC
CCCGGTATTCTTCGATGAGC

Product: sigma-54-dependent transcriptional response regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MTDSSTRILIIDDERDVCTFFSRLLSRKGYQVVTAASEPEALSALEAAQFSVALVDLKLPDTDGITLLEIIKSRQPACEV
IIMTGYSTVKTAVAAMQLGAYEYLEKPFDDIDQIEQLVGRAAGSGASLLRGDNSHDEWAEVAQGVGFRVGSSPSMKRMVS
LAYKVAGKDISVLIQGKTGTGKEVLARFIHAASCRAGQPFIPVNCGALPENLLEGELFGHEKGAFTGANQTRRGIFELAN
NGTLLLDEIGDATPQIQVKLLRVLETGEFMRVGGERPIRTDVRVIAATNVDLEQAIAEKSFREDLFYRLNVVRLEIPSLS
ARSEDIPLLAEHFVHQINRELKLAAGTLRMLQGYAWPGNIRELANVMRRAVVICNGDTILPEHLGGTFLAGPTAPERGSA
AKTIQQPDRSRLQATLLEHCDSAEELERLDAEELNRLLDSLRQAQSNLLGVMRKKKIVPALHALKDSEAETIKEALAQYD
GNITEAARALGIARNTLYRKIKELGLPGR

Sequences:

>Translated_509_residues
MTDSSTRILIIDDERDVCTFFSRLLSRKGYQVVTAASEPEALSALEAAQFSVALVDLKLPDTDGITLLEIIKSRQPACEV
IIMTGYSTVKTAVAAMQLGAYEYLEKPFDDIDQIEQLVGRAAGSGASLLRGDNSHDEWAEVAQGVGFRVGSSPSMKRMVS
LAYKVAGKDISVLIQGKTGTGKEVLARFIHAASCRAGQPFIPVNCGALPENLLEGELFGHEKGAFTGANQTRRGIFELAN
NGTLLLDEIGDATPQIQVKLLRVLETGEFMRVGGERPIRTDVRVIAATNVDLEQAIAEKSFREDLFYRLNVVRLEIPSLS
ARSEDIPLLAEHFVHQINRELKLAAGTLRMLQGYAWPGNIRELANVMRRAVVICNGDTILPEHLGGTFLAGPTAPERGSA
AKTIQQPDRSRLQATLLEHCDSAEELERLDAEELNRLLDSLRQAQSNLLGVMRKKKIVPALHALKDSEAETIKEALAQYD
GNITEAARALGIARNTLYRKIKELGLPGR
>Mature_508_residues
TDSSTRILIIDDERDVCTFFSRLLSRKGYQVVTAASEPEALSALEAAQFSVALVDLKLPDTDGITLLEIIKSRQPACEVI
IMTGYSTVKTAVAAMQLGAYEYLEKPFDDIDQIEQLVGRAAGSGASLLRGDNSHDEWAEVAQGVGFRVGSSPSMKRMVSL
AYKVAGKDISVLIQGKTGTGKEVLARFIHAASCRAGQPFIPVNCGALPENLLEGELFGHEKGAFTGANQTRRGIFELANN
GTLLLDEIGDATPQIQVKLLRVLETGEFMRVGGERPIRTDVRVIAATNVDLEQAIAEKSFREDLFYRLNVVRLEIPSLSA
RSEDIPLLAEHFVHQINRELKLAAGTLRMLQGYAWPGNIRELANVMRRAVVICNGDTILPEHLGGTFLAGPTAPERGSAA
KTIQQPDRSRLQATLLEHCDSAEELERLDAEELNRLLDSLRQAQSNLLGVMRKKKIVPALHALKDSEAETIKEALAQYDG
NITEAARALGIARNTLYRKIKELGLPGR

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=510, Percent_Identity=40.3921568627451, Blast_Score=333, Evalue=2e-92,
Organism=Escherichia coli, GI1788550, Length=506, Percent_Identity=39.9209486166008, Blast_Score=328, Evalue=5e-91,
Organism=Escherichia coli, GI1790437, Length=384, Percent_Identity=45.5729166666667, Blast_Score=315, Evalue=6e-87,
Organism=Escherichia coli, GI1788905, Length=402, Percent_Identity=41.5422885572139, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI87082117, Length=241, Percent_Identity=51.8672199170125, Blast_Score=247, Evalue=2e-66,
Organism=Escherichia coli, GI1789087, Length=348, Percent_Identity=41.0919540229885, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1789233, Length=365, Percent_Identity=38.9041095890411, Blast_Score=228, Evalue=1e-60,
Organism=Escherichia coli, GI87082152, Length=391, Percent_Identity=38.3631713554987, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1787583, Length=275, Percent_Identity=42.5454545454545, Blast_Score=201, Evalue=8e-53,
Organism=Escherichia coli, GI1786524, Length=269, Percent_Identity=43.1226765799257, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI87081872, Length=229, Percent_Identity=47.5982532751092, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI1789828, Length=247, Percent_Identity=36.4372469635627, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87081858, Length=245, Percent_Identity=32.6530612244898, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790552, Length=144, Percent_Identity=28.4722222222222, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 55636; Mature: 55505

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSSTRILIIDDERDVCTFFSRLLSRKGYQVVTAASEPEALSALEAAQFSVALVDLKLP
CCCCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHEEEEEEEEECC
DTDGITLLEIIKSRQPACEVIIMTGYSTVKTAVAAMQLGAYEYLEKPFDDIDQIEQLVGR
CCCCHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AAGSGASLLRGDNSHDEWAEVAQGVGFRVGSSPSMKRMVSLAYKVAGKDISVLIQGKTGT
CCCCCCHHHCCCCCCHHHHHHHHCCCEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCC
GKEVLARFIHAASCRAGQPFIPVNCGALPENLLEGELFGHEKGAFTGANQTRRGIFELAN
CHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHCCHHCCCCCCCCCCCCHHHHHHHHHHC
NGTLLLDEIGDATPQIQVKLLRVLETGEFMRVGGERPIRTDVRVIAATNVDLEQAIAEKS
CCEEEEECCCCCCHHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEEECCCCHHHHHHHHH
FREDLFYRLNVVRLEIPSLSARSEDIPLLAEHFVHQINRELKLAAGTLRMLQGYAWPGNI
HHHHHHHHEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
RELANVMRRAVVICNGDTILPEHLGGTFLAGPTAPERGSAAKTIQQPDRSRLQATLLEHC
HHHHHHHHHEEEEECCCEECHHHCCCCEEECCCCCCCCCHHHHHHCCHHHHHHHHHHHHC
DSAEELERLDAEELNRLLDSLRQAQSNLLGVMRKKKIVPALHALKDSEAETIKEALAQYD
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
GNITEAARALGIARNTLYRKIKELGLPGR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TDSSTRILIIDDERDVCTFFSRLLSRKGYQVVTAASEPEALSALEAAQFSVALVDLKLP
CCCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHEEEEEEEEECC
DTDGITLLEIIKSRQPACEVIIMTGYSTVKTAVAAMQLGAYEYLEKPFDDIDQIEQLVGR
CCCCHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AAGSGASLLRGDNSHDEWAEVAQGVGFRVGSSPSMKRMVSLAYKVAGKDISVLIQGKTGT
CCCCCCHHHCCCCCCHHHHHHHHCCCEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCC
GKEVLARFIHAASCRAGQPFIPVNCGALPENLLEGELFGHEKGAFTGANQTRRGIFELAN
CHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHCCHHCCCCCCCCCCCCHHHHHHHHHHC
NGTLLLDEIGDATPQIQVKLLRVLETGEFMRVGGERPIRTDVRVIAATNVDLEQAIAEKS
CCEEEEECCCCCCHHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEEECCCCHHHHHHHHH
FREDLFYRLNVVRLEIPSLSARSEDIPLLAEHFVHQINRELKLAAGTLRMLQGYAWPGNI
HHHHHHHHEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
RELANVMRRAVVICNGDTILPEHLGGTFLAGPTAPERGSAAKTIQQPDRSRLQATLLEHC
HHHHHHHHHEEEEECCCEECHHHCCCCEEECCCCCCCCCHHHHHHCCHHHHHHHHHHHHC
DSAEELERLDAEELNRLLDSLRQAQSNLLGVMRKKKIVPALHALKDSEAETIKEALAQYD
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
GNITEAARALGIARNTLYRKIKELGLPGR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8095910 [H]