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The map label for this gene is atoS [H]

Identifier: 197118369

GI number: 197118369

Start: 2290466

End: 2292124

Strand: Direct

Name: atoS [H]

Synonym: Gbem_1987

Alternate gene names: 197118369

Gene position: 2290466-2292124 (Clockwise)

Preceding gene: 197118368

Following gene: 197118370

Centisome position: 49.63

GC content: 61.0

Gene sequence:

>1659_bases
GTGAAGAACAGGGGCAAACTGCTGGAGCAGTTGACGGGGGTCGATTCCTCCAAGCTCAACTACTACGTCGAGCTCAAAAA
GAGAAACCAGGACGTGCTGAAGCAAAACAGCCGCCTGGAGATCCTGCAGCAGCTCACGCGCGACATGAACATCGACATGT
CCATCGGCGACATCATCGAAAGGACGTTCAAAAAGCTCCCCGAGGCGCTCCCCTGCGACTTCCTGGGGCTGGCGAAGTTG
AGCGACGGCAAGTTGAAGCTGATCGCGGTCGCCCCCAGGGAATTTTCCACGCTCGGTTTCATACCCAAGGAGTCCGTCCT
CTGGGACGCGTTCCGGGACAAGGGGGCGACCAGCTACGAACCGGCCGACGACCCCCTTTGCTTTGCCCAGATTGCCGCCG
AACATCCCCTGACCCTGCGCTCCCTGGCCGCGGCGCCGCTTTTCGAGCGCTCCTCGGTCAACGGGCTGTTGCTTTTGGGA
AGCGCGCTCGAGTCCGCCTACGAGGGGGCGGAGCTGAACTTCGTGCAGCACCTGGCCGACCAGCTCGCCATCAGCATGCA
GAACGCGCGGCTCTACAAGCAGGTTTCCCGCGCCAAGAAGGAGTGGGAGGCGACCTTCAAGGCCGTGACCGACCCCATCT
TCCTGATCGATACCGATTACAACGTGCTTTTGCACAACGACCGTCTTCCCCCCGGCATGGCCGAGATGTGGAACAGCTCC
ATCAGCAAGAAGTGCTTCGCGAAGATGCGCGGCGAAAACGGGCCCTGCCGCAACTGCCCCATCCAGGAGATCAAGGAGAC
CGGCAACCCCGTGTTCCAGCAGTGGAACACCCCGCAGGGCGAGTGCCTGGATATGTCCTATTACCCGGTCTTCAACGAGG
AGAAGCAGCTCTCCGCGGTCACCATCATCATGAAGGACGTCACCAAGAGGCTGAAGATGGAGGCCCAACTGGTGCAGTCG
GCCAAGATGGTGGCCTTGGGCGTCATGGCGGCGGGGGTGGCGCACGAGCTGAACAGCCCGATGACCGTGATCATCGGGAC
CGCCCAGATGCTCGCCAGGGAGATGCAGCAGGAAGGGGATTCCGAAGGGGCCGAGGCCTTGTCCGACATCATCAACTGCG
GCCTGCGCTGCAAGAGGATCATCCAGAACCTCCTTACCTTCTCGCGCCAGGACCAGGTCCCGGTCATGGCTATCGATCTG
AACGCCGAGGTGGAGCGGGTGCTCGACATGATCCAGTACCAGATCAACCGGAGCCAGATCGCCATCTTCGAGCATTTCGA
CCGCGACATACCGATGATGAACGCGAACGGGCCGCAGATCCAGCAGGTGCTGACTAACTTCCTCATCAATGCGCGAGACG
CCCTGATGGAGGTGGAAGGGAAGGAGAAGGTCATCGAGGTCTCCACCGTGCTTCGGGTCGAGGGGGAGCGGCGCTTTGCC
GTCTTAAGCGTCGCCGACAACGGCATCGGCATCGCGAAGGAAAACCTCTCCAAGATCTTCACCCCTTTCTACACCAGCAA
AGAGGCCTCCAAGGGGACCGGGCTCGGCCTTTCGGTGAGCCTCGGCATAGCGGAGTCCCACAACGGCCGCATCGAGGTCG
ACACCGAACTTGGGCAGGGGAGCTGTTTCAGGTTGATTCTTCCCGTCGACCAGCAGTAA

Upstream 100 bases:

>100_bases
GCCTGCCTCCTCACCAACCCGCGCAGCGCCACCAGAGAGCAGATCGAGGAGATCTACCGCAACGCCATGTAAGTGCGGCC
GAGGCCGCGAAGGAAAGAAC

Downstream 100 bases:

>100_bases
GCAGACATATATATTGGAGCAACCATGACGGACTCATCTACTAGAATACTTATCATCGACGACGAGAGGGATGTCTGCAC
CTTTTTCAGCCGGCTTTTGT

Product: GAF domain-containing sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MKNRGKLLEQLTGVDSSKLNYYVELKKRNQDVLKQNSRLEILQQLTRDMNIDMSIGDIIERTFKKLPEALPCDFLGLAKL
SDGKLKLIAVAPREFSTLGFIPKESVLWDAFRDKGATSYEPADDPLCFAQIAAEHPLTLRSLAAAPLFERSSVNGLLLLG
SALESAYEGAELNFVQHLADQLAISMQNARLYKQVSRAKKEWEATFKAVTDPIFLIDTDYNVLLHNDRLPPGMAEMWNSS
ISKKCFAKMRGENGPCRNCPIQEIKETGNPVFQQWNTPQGECLDMSYYPVFNEEKQLSAVTIIMKDVTKRLKMEAQLVQS
AKMVALGVMAAGVAHELNSPMTVIIGTAQMLAREMQQEGDSEGAEALSDIINCGLRCKRIIQNLLTFSRQDQVPVMAIDL
NAEVERVLDMIQYQINRSQIAIFEHFDRDIPMMNANGPQIQQVLTNFLINARDALMEVEGKEKVIEVSTVLRVEGERRFA
VLSVADNGIGIAKENLSKIFTPFYTSKEASKGTGLGLSVSLGIAESHNGRIEVDTELGQGSCFRLILPVDQQ

Sequences:

>Translated_552_residues
MKNRGKLLEQLTGVDSSKLNYYVELKKRNQDVLKQNSRLEILQQLTRDMNIDMSIGDIIERTFKKLPEALPCDFLGLAKL
SDGKLKLIAVAPREFSTLGFIPKESVLWDAFRDKGATSYEPADDPLCFAQIAAEHPLTLRSLAAAPLFERSSVNGLLLLG
SALESAYEGAELNFVQHLADQLAISMQNARLYKQVSRAKKEWEATFKAVTDPIFLIDTDYNVLLHNDRLPPGMAEMWNSS
ISKKCFAKMRGENGPCRNCPIQEIKETGNPVFQQWNTPQGECLDMSYYPVFNEEKQLSAVTIIMKDVTKRLKMEAQLVQS
AKMVALGVMAAGVAHELNSPMTVIIGTAQMLAREMQQEGDSEGAEALSDIINCGLRCKRIIQNLLTFSRQDQVPVMAIDL
NAEVERVLDMIQYQINRSQIAIFEHFDRDIPMMNANGPQIQQVLTNFLINARDALMEVEGKEKVIEVSTVLRVEGERRFA
VLSVADNGIGIAKENLSKIFTPFYTSKEASKGTGLGLSVSLGIAESHNGRIEVDTELGQGSCFRLILPVDQQ
>Mature_552_residues
MKNRGKLLEQLTGVDSSKLNYYVELKKRNQDVLKQNSRLEILQQLTRDMNIDMSIGDIIERTFKKLPEALPCDFLGLAKL
SDGKLKLIAVAPREFSTLGFIPKESVLWDAFRDKGATSYEPADDPLCFAQIAAEHPLTLRSLAAAPLFERSSVNGLLLLG
SALESAYEGAELNFVQHLADQLAISMQNARLYKQVSRAKKEWEATFKAVTDPIFLIDTDYNVLLHNDRLPPGMAEMWNSS
ISKKCFAKMRGENGPCRNCPIQEIKETGNPVFQQWNTPQGECLDMSYYPVFNEEKQLSAVTIIMKDVTKRLKMEAQLVQS
AKMVALGVMAAGVAHELNSPMTVIIGTAQMLAREMQQEGDSEGAEALSDIINCGLRCKRIIQNLLTFSRQDQVPVMAIDL
NAEVERVLDMIQYQINRSQIAIFEHFDRDIPMMNANGPQIQQVLTNFLINARDALMEVEGKEKVIEVSTVLRVEGERRFA
VLSVADNGIGIAKENLSKIFTPFYTSKEASKGTGLGLSVSLGIAESHNGRIEVDTELGQGSCFRLILPVDQQ

Specific function: Member of the two-component regulatory system AtoS/AtoC; may activate AtoC by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=270, Percent_Identity=33.3333333333333, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1790436, Length=242, Percent_Identity=33.4710743801653, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1790300, Length=250, Percent_Identity=30, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786912, Length=267, Percent_Identity=26.2172284644195, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI145693157, Length=264, Percent_Identity=26.1363636363636, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786600, Length=282, Percent_Identity=25.886524822695, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI48994928, Length=295, Percent_Identity=24.0677966101695, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081816, Length=248, Percent_Identity=27.4193548387097, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1789808, Length=233, Percent_Identity=30.0429184549356, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 61541; Mature: 61541

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNRGKLLEQLTGVDSSKLNYYVELKKRNQDVLKQNSRLEILQQLTRDMNIDMSIGDIIE
CCCHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHH
RTFKKLPEALPCDFLGLAKLSDGKLKLIAVAPREFSTLGFIPKESVLWDAFRDKGATSYE
HHHHHCCHHCCCCCCCCCCCCCCCEEEEEECCCCCHHCCCCCCHHHHHHHHHCCCCCCCC
PADDPLCFAQIAAEHPLTLRSLAAAPLFERSSVNGLLLLGSALESAYEGAELNFVQHLAD
CCCCCHHHHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEECHHHHHHHCCCHHHHHHHHHH
QLAISMQNARLYKQVSRAKKEWEATFKAVTDPIFLIDTDYNVLLHNDRLPPGMAEMWNSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCHHHHHHHH
ISKKCFAKMRGENGPCRNCPIQEIKETGNPVFQQWNTPQGECLDMSYYPVFNEEKQLSAV
HHHHHHHHHCCCCCCCCCCCHHHHHHCCCHHHHCCCCCCCCEEEEEECCCCCCCCHHHHH
TIIMKDVTKRLKMEAQLVQSAKMVALGVMAAGVAHELNSPMTVIIGTAQMLAREMQQEGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHCCC
SEGAEALSDIINCGLRCKRIIQNLLTFSRQDQVPVMAIDLNAEVERVLDMIQYQINRSQI
CHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCHHE
AIFEHFDRDIPMMNANGPQIQQVLTNFLINARDALMEVEGKEKVIEVSTVLRVEGERRFA
EHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEHHEEECCCCEEE
VLSVADNGIGIAKENLSKIFTPFYTSKEASKGTGLGLSVSLGIAESHNGRIEVDTELGQG
EEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCEEEEECCCCCC
SCFRLILPVDQQ
CEEEEEEECCCC
>Mature Secondary Structure
MKNRGKLLEQLTGVDSSKLNYYVELKKRNQDVLKQNSRLEILQQLTRDMNIDMSIGDIIE
CCCHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHH
RTFKKLPEALPCDFLGLAKLSDGKLKLIAVAPREFSTLGFIPKESVLWDAFRDKGATSYE
HHHHHCCHHCCCCCCCCCCCCCCCEEEEEECCCCCHHCCCCCCHHHHHHHHHCCCCCCCC
PADDPLCFAQIAAEHPLTLRSLAAAPLFERSSVNGLLLLGSALESAYEGAELNFVQHLAD
CCCCCHHHHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEECHHHHHHHCCCHHHHHHHHHH
QLAISMQNARLYKQVSRAKKEWEATFKAVTDPIFLIDTDYNVLLHNDRLPPGMAEMWNSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCHHHHHHHH
ISKKCFAKMRGENGPCRNCPIQEIKETGNPVFQQWNTPQGECLDMSYYPVFNEEKQLSAV
HHHHHHHHHCCCCCCCCCCCHHHHHHCCCHHHHCCCCCCCCEEEEEECCCCCCCCHHHHH
TIIMKDVTKRLKMEAQLVQSAKMVALGVMAAGVAHELNSPMTVIIGTAQMLAREMQQEGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHCCC
SEGAEALSDIINCGLRCKRIIQNLLTFSRQDQVPVMAIDLNAEVERVLDMIQYQINRSQI
CHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCHHE
AIFEHFDRDIPMMNANGPQIQQVLTNFLINARDALMEVEGKEKVIEVSTVLRVEGERRFA
EHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEHHEEECCCCEEE
VLSVADNGIGIAKENLSKIFTPFYTSKEASKGTGLGLSVSLGIAESHNGRIEVDTELGQG
EEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCEEEEECCCCCC
SCFRLILPVDQQ
CEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8346225; 9097040; 9278503 [H]