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The map label for this gene is dhaT [H]

Identifier: 197118368

GI number: 197118368

Start: 2289289

End: 2290437

Strand: Direct

Name: dhaT [H]

Synonym: Gbem_1986

Alternate gene names: 197118368

Gene position: 2289289-2290437 (Clockwise)

Preceding gene: 197118367

Following gene: 197118369

Centisome position: 49.6

GC content: 63.27

Gene sequence:

>1149_bases
ATGGAAATAAGCAAGTTTGTAGCGCCTGAAATCATCTTCGGGCGGGGCTCTTTGAGCCAGATCGGCGAGAGCGTGGTCCG
CCTGGGCGTCTCCAAGGTGTTCATGGTGAGCGACGACCATGTCATCGCCGCCGGGTGGGTGGATCAGGCCGTGGAATACC
TGCGCGCCGCAGGGCTTGAGACGGTGATCTTCTCCAGCCTCACCACCAACCCGAAGGACCGCGAGGTGACCGAGGGGACG
GCGAAGTACCTCTCTTCCGGATGCGACGGCATTGTCTCCGTCGGGGGTGGAAGCCCGACCGACGTCGCCAAGGCGATCGC
CATCATCGCCTCCAACGGCGGGAAGCTGCAGGATTACGAGGGAGTGAATAAGATCAGCTTGCCGCTCCCCCCGATGGTCA
TCGTCCCCAGCACCGCCGGCGCGGGCTCGGAGGTGAGCCAGTTCGCCATCATCGTCGACACCAAAAGAAAGCTGAAGATG
TCGATCATCTCCAGGTCGCTCATCCCCGATATCGCCATCGTCGATCCGGAACTTCTGAAGACCAAGGACGCCAAACTAGC
AGCCGCCACCGGGGTCGATGCGCTCACCCACGGCATCGAGTCCTACGTATCGCTGGCGGCAACCCCTTTAACCGACATAC
ATGCACTGAAGGCGATCCAGCTCATCTCGAAGAACCTGAGAAGGGCGGTGGCCGTGCGCAGCGACATGGACGCCAACACC
AACATGGCCATGGCCAGCCTCACCGCGGGGCTCGCCTTTTCCAACGCCATCCTGGGCGCCGCCCACGCCATGACCCACCA
GGTGGACGGCCTGCTCGACCAGCACCACGGCGAAACCAACGCCTCCATCCTGCCGCACGTGATGCGCTTCAACTTGCAGG
CATGCCCGGAGCGCTTCCGCGACATCGCCGTCGCCATGGGCGAGGACGTGACCGGTCTCGAGCTCATGGCCGCGGCCGAG
CGTTCCATCGATGCGGTTAAGGCCCTCATCGCCGAGATCGGGCTGGACCAGGGGCTCTCCGAACTGGGGCTCCGGGAGGA
GTTCATCCCGCTTCTGAGCGAAAACGCCACCAAGGACGCCTGCCTCCTCACCAACCCGCGCAGCGCCACCAGAGAGCAGA
TCGAGGAGATCTACCGCAACGCCATGTAA

Upstream 100 bases:

>100_bases
AAATATCGCCGCGCCGTCGCCGGCACCCCAAGGTTGGTACCGATCTTGCCTGATGTAAACACAATCACGACGGATGCAAC
ACCGCTTTAGGGAGTCGAAA

Downstream 100 bases:

>100_bases
GTGCGGCCGAGGCCGCGAAGGAAAGAACGTGAAGAACAGGGGCAAACTGCTGGAGCAGTTGACGGGGGTCGATTCCTCCA
AGCTCAACTACTACGTCGAG

Product: iron-containing alcohol dehydrogenase

Products: NA

Alternate protein names: 1,3-propanediol oxidoreductase; 3-hydroxypropionaldehyde reductase [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MEISKFVAPEIIFGRGSLSQIGESVVRLGVSKVFMVSDDHVIAAGWVDQAVEYLRAAGLETVIFSSLTTNPKDREVTEGT
AKYLSSGCDGIVSVGGGSPTDVAKAIAIIASNGGKLQDYEGVNKISLPLPPMVIVPSTAGAGSEVSQFAIIVDTKRKLKM
SIISRSLIPDIAIVDPELLKTKDAKLAAATGVDALTHGIESYVSLAATPLTDIHALKAIQLISKNLRRAVAVRSDMDANT
NMAMASLTAGLAFSNAILGAAHAMTHQVDGLLDQHHGETNASILPHVMRFNLQACPERFRDIAVAMGEDVTGLELMAAAE
RSIDAVKALIAEIGLDQGLSELGLREEFIPLLSENATKDACLLTNPRSATREQIEEIYRNAM

Sequences:

>Translated_382_residues
MEISKFVAPEIIFGRGSLSQIGESVVRLGVSKVFMVSDDHVIAAGWVDQAVEYLRAAGLETVIFSSLTTNPKDREVTEGT
AKYLSSGCDGIVSVGGGSPTDVAKAIAIIASNGGKLQDYEGVNKISLPLPPMVIVPSTAGAGSEVSQFAIIVDTKRKLKM
SIISRSLIPDIAIVDPELLKTKDAKLAAATGVDALTHGIESYVSLAATPLTDIHALKAIQLISKNLRRAVAVRSDMDANT
NMAMASLTAGLAFSNAILGAAHAMTHQVDGLLDQHHGETNASILPHVMRFNLQACPERFRDIAVAMGEDVTGLELMAAAE
RSIDAVKALIAEIGLDQGLSELGLREEFIPLLSENATKDACLLTNPRSATREQIEEIYRNAM
>Mature_382_residues
MEISKFVAPEIIFGRGSLSQIGESVVRLGVSKVFMVSDDHVIAAGWVDQAVEYLRAAGLETVIFSSLTTNPKDREVTEGT
AKYLSSGCDGIVSVGGGSPTDVAKAIAIIASNGGKLQDYEGVNKISLPLPPMVIVPSTAGAGSEVSQFAIIVDTKRKLKM
SIISRSLIPDIAIVDPELLKTKDAKLAAATGVDALTHGIESYVSLAATPLTDIHALKAIQLISKNLRRAVAVRSDMDANT
NMAMASLTAGLAFSNAILGAAHAMTHQVDGLLDQHHGETNASILPHVMRFNLQACPERFRDIAVAMGEDVTGLELMAAAE
RSIDAVKALIAEIGLDQGLSELGLREEFIPLLSENATKDACLLTNPRSATREQIEEIYRNAM

Specific function: Most active with substrates containing two primary alcohol groups separated by one or two carbon atoms. In the physiological direction, 3-hydroxypropionaldehyde is the preferred substrate [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=381, Percent_Identity=27.0341207349081, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI48994951, Length=383, Percent_Identity=40.9921671018277, Blast_Score=295, Evalue=5e-81,
Organism=Escherichia coli, GI1789163, Length=372, Percent_Identity=38.1720430107527, Blast_Score=259, Evalue=1e-70,
Organism=Escherichia coli, GI87082107, Length=376, Percent_Identity=36.1702127659575, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI1787493, Length=398, Percent_Identity=30.4020100502513, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1789386, Length=359, Percent_Identity=22.8412256267409, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17537053, Length=420, Percent_Identity=27.1428571428571, Blast_Score=147, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6321181, Length=382, Percent_Identity=37.9581151832461, Blast_Score=248, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24657991, Length=414, Percent_Identity=25.8454106280193, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.202 [H]

Molecular weight: Translated: 40500; Mature: 40500

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEISKFVAPEIIFGRGSLSQIGESVVRLGVSKVFMVSDDHVIAAGWVDQAVEYLRAAGLE
CCCHHHHCCCEEECCCCHHHHHHHHHHHHHHEEEEECCCCEEEEHHHHHHHHHHHHCCHH
TVIFSSLTTNPKDREVTEGTAKYLSSGCDGIVSVGGGSPTDVAKAIAIIASNGGKLQDYE
HHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCC
GVNKISLPLPPMVIVPSTAGAGSEVSQFAIIVDTKRKLKMSIISRSLIPDIAIVDPELLK
CCCEEECCCCCEEEEECCCCCCCCHHEEEEEEECHHHHHHHHHHHHHCCCEEECCHHHHH
TKDAKLAAATGVDALTHGIESYVSLAATPLTDIHALKAIQLISKNLRRAVAVRSDMDANT
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NMAMASLTAGLAFSNAILGAAHAMTHQVDGLLDQHHGETNASILPHVMRFNLQACPERFR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHHHHHH
DIAVAMGEDVTGLELMAAAERSIDAVKALIAEIGLDQGLSELGLREEFIPLLSENATKDA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHCCHHHHCCHHHHHHHHCCCCCCCE
CLLTNPRSATREQIEEIYRNAM
EEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEISKFVAPEIIFGRGSLSQIGESVVRLGVSKVFMVSDDHVIAAGWVDQAVEYLRAAGLE
CCCHHHHCCCEEECCCCHHHHHHHHHHHHHHEEEEECCCCEEEEHHHHHHHHHHHHCCHH
TVIFSSLTTNPKDREVTEGTAKYLSSGCDGIVSVGGGSPTDVAKAIAIIASNGGKLQDYE
HHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCC
GVNKISLPLPPMVIVPSTAGAGSEVSQFAIIVDTKRKLKMSIISRSLIPDIAIVDPELLK
CCCEEECCCCCEEEEECCCCCCCCHHEEEEEEECHHHHHHHHHHHHHCCCEEECCHHHHH
TKDAKLAAATGVDALTHGIESYVSLAATPLTDIHALKAIQLISKNLRRAVAVRSDMDANT
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NMAMASLTAGLAFSNAILGAAHAMTHQVDGLLDQHHGETNASILPHVMRFNLQACPERFR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHHHHHH
DIAVAMGEDVTGLELMAAAERSIDAVKALIAEIGLDQGLSELGLREEFIPLLSENATKDA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHCCHHHHCCHHHHHHHHCCCCCCCE
CLLTNPRSATREQIEEIYRNAM
EEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7721705 [H]