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The map label for this gene is yefM [H]

Identifier: 197118310

GI number: 197118310

Start: 2228585

End: 2228836

Strand: Reverse

Name: yefM [H]

Synonym: Gbem_1927

Alternate gene names: 197118310

Gene position: 2228836-2228585 (Counterclockwise)

Preceding gene: 197118311

Following gene: 197118309

Centisome position: 48.29

GC content: 54.76

Gene sequence:

>252_bases
ATGAATTCGATAACCTACAGTTCCGTGCGCAGCAAACTGGCAGAAACGATGGAGAAGGTATGTGACGACCATGCCCCCGT
CATCATCACCCGCAAAGCGGCAAAGCCGGTGGTCATGATGTCACTTGACGACTACGAAGCGCTGGAGGAAACGGCGTACC
TGCTGCGAAGCCCTAAAAACGTGCGGCGGCTGGTTGAATCTATAGCCGAGCTAGAGACCGGAAAGGCAACCGAACGGGAG
CTGATCGATTGA

Upstream 100 bases:

>100_bases
GGACCTCCGCGACCTCTGTGTTCCGCCTTTCGGTTATTCAATTGTCAATCCTCAATTAGTATTGTACGATTACTTGTACA
ACTATCGCAGGAGTTAGATC

Downstream 100 bases:

>100_bases
AGCTCATATTCTCGGAAAGCGCTTGGGAAGATTACCTGTTCTGGCAGAGCACAGACAAGAAGGTCGCCAAGCGCATCAAC
ACCCTGATCAGGGAGATCCA

Product: antitoxin YefM, prevent-host-death family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 83; Mature: 83

Protein sequence:

>83_residues
MNSITYSSVRSKLAETMEKVCDDHAPVIITRKAAKPVVMMSLDDYEALEETAYLLRSPKNVRRLVESIAELETGKATERE
LID

Sequences:

>Translated_83_residues
MNSITYSSVRSKLAETMEKVCDDHAPVIITRKAAKPVVMMSLDDYEALEETAYLLRSPKNVRRLVESIAELETGKATERE
LID
>Mature_83_residues
MNSITYSSVRSKLAETMEKVCDDHAPVIITRKAAKPVVMMSLDDYEALEETAYLLRSPKNVRRLVESIAELETGKATERE
LID

Specific function: Part of a toxin-antitoxin (TA) module. Antitoxin that counteracts the effect of the YoeB mRNA interferase. YefM binds to the promoter region of the yefM-yeoB operon to repress transcription, YeoB acts as a corepressor [H]

COG id: COG2161

COG function: function code D; Antitoxin of toxin-antitoxin stability system

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the YefM antitoxin family [H]

Homologues:

Organism=Escherichia coli, GI87082026, Length=83, Percent_Identity=53.0120481927711, Blast_Score=99, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006442 [H]

Pfam domain/function: PF02604 PhdYeFM [H]

EC number: NA

Molecular weight: Translated: 9372; Mature: 9372

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSITYSSVRSKLAETMEKVCDDHAPVIITRKAAKPVVMMSLDDYEALEETAYLLRSPKN
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEECCHHHHHHHHHHHHHCCHH
VRRLVESIAELETGKATERELID
HHHHHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure
MNSITYSSVRSKLAETMEKVCDDHAPVIITRKAAKPVVMMSLDDYEALEETAYLLRSPKN
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEECCHHHHHHHHHHHHHCCHH
VRRLVESIAELETGKATERELID
HHHHHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12384590; 12704152 [H]