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The map label for this gene is phoB-1 [H]

Identifier: 197118248

GI number: 197118248

Start: 2158758

End: 2159438

Strand: Reverse

Name: phoB-1 [H]

Synonym: Gbem_1865

Alternate gene names: 197118248

Gene position: 2159438-2158758 (Counterclockwise)

Preceding gene: 197118249

Following gene: 197118247

Centisome position: 46.79

GC content: 57.42

Gene sequence:

>681_bases
ATGCAAACTGTATTGATCATTGAAGATGAGAAAGACCTCGCGGAGTTGCTTTCTTTCAACCTTGAACGGGAAGGATACAA
GACAGTGATAGCTCTCGACGGCGCCGAGGGGTTGGAGGCCGTGGAGCGGGTTCAGCCTGACCTTATCCTTTTGGACCTGA
TGTTGCCCGGGATGTTCGGCACCGACGTGTGCAAGACGGTCAAGAAATCCGAAAAGTCGGCGCACATCCCGGTGGTCATG
CTGACTGCGAAGGGGGAGGAGATAGACAAAGTGGTCGGCTTCGAGGTCGGCGCCGACGACTACGTGATCAAGCCGTTTTC
CACCAGAGAACTCATGCTCCGCGTCAAGGCGGTGCTGCGCCGAAAGGCACCAGAACCGGCGGCGACCAACATCATCAACG
TTGGCGGGATCAGAATCGACACCGAGCGCCACGAAGTCACCGTGAACGGAGACGAGATCAATTTCACCACAACCGAGTTC
AAGCTCCTGTACACCCTGGTGATGCGCTTTGGCAGGGTCCAAAGCCGCGACGTACTGCTGCGCGACGTATGGGGCTACAA
CTTCGTCGAGGACACCCGCACCGTCGACACCCACATCACCCGCCTGCGGACCAAGATGGGCAACGCGGGAGATATGATCA
AGACGGTGCGCGGCTTCGGCTACAAATTGGAGGTGCAATGA

Upstream 100 bases:

>100_bases
TGACAGAGCTGTAACGGTTTCTAAACAAAGAAGTTATATGAAGTATACGAGCCATGACTTCACTGCCTGACAAGCATGAC
GAACCTTAGAGAGGGATGCG

Downstream 100 bases:

>100_bases
GGTTAAAAGTCATCGGCATCCAGTTCGCAGTCGTCATGGCGTTTGCTGCGGTACTTTGCAGCTACGAGCAGCGCCTGCTC
GGAGTTGGCTGGGCTTTGCT

Product: winged-helix phosphate transcriptional response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MQTVLIIEDEKDLAELLSFNLEREGYKTVIALDGAEGLEAVERVQPDLILLDLMLPGMFGTDVCKTVKKSEKSAHIPVVM
LTAKGEEIDKVVGFEVGADDYVIKPFSTRELMLRVKAVLRRKAPEPAATNIINVGGIRIDTERHEVTVNGDEINFTTTEF
KLLYTLVMRFGRVQSRDVLLRDVWGYNFVEDTRTVDTHITRLRTKMGNAGDMIKTVRGFGYKLEVQ

Sequences:

>Translated_226_residues
MQTVLIIEDEKDLAELLSFNLEREGYKTVIALDGAEGLEAVERVQPDLILLDLMLPGMFGTDVCKTVKKSEKSAHIPVVM
LTAKGEEIDKVVGFEVGADDYVIKPFSTRELMLRVKAVLRRKAPEPAATNIINVGGIRIDTERHEVTVNGDEINFTTTEF
KLLYTLVMRFGRVQSRDVLLRDVWGYNFVEDTRTVDTHITRLRTKMGNAGDMIKTVRGFGYKLEVQ
>Mature_226_residues
MQTVLIIEDEKDLAELLSFNLEREGYKTVIALDGAEGLEAVERVQPDLILLDLMLPGMFGTDVCKTVKKSEKSAHIPVVM
LTAKGEEIDKVVGFEVGADDYVIKPFSTRELMLRVKAVLRRKAPEPAATNIINVGGIRIDTERHEVTVNGDEINFTTTEF
KLLYTLVMRFGRVQSRDVLLRDVWGYNFVEDTRTVDTHITRLRTKMGNAGDMIKTVRGFGYKLEVQ

Specific function: Part of the two-component regulatory system senX3/regX3. Once phosphorylated by senX3, activates the expression of several operons/genes involved in phosphate assimilation and metabolism, such as pstSCAB, phnDCE, and phoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=225, Percent_Identity=43.5555555555556, Blast_Score=183, Evalue=8e-48,
Organism=Escherichia coli, GI1788394, Length=226, Percent_Identity=40.2654867256637, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI87082012, Length=222, Percent_Identity=37.3873873873874, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI2367329, Length=230, Percent_Identity=37.3913043478261, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1790860, Length=227, Percent_Identity=36.5638766519824, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1786784, Length=224, Percent_Identity=37.5, Blast_Score=138, Evalue=2e-34,
Organism=Escherichia coli, GI1789809, Length=226, Percent_Identity=34.5132743362832, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=34.5454545454545, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1789402, Length=220, Percent_Identity=32.7272727272727, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1786911, Length=206, Percent_Identity=33.0097087378641, Blast_Score=117, Evalue=4e-28,
Organism=Escherichia coli, GI1787229, Length=227, Percent_Identity=34.8017621145374, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1790863, Length=235, Percent_Identity=31.063829787234, Blast_Score=113, Evalue=8e-27,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=31.1111111111111, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI145693140, Length=234, Percent_Identity=31.1965811965812, Blast_Score=110, Evalue=6e-26,
Organism=Escherichia coli, GI1788550, Length=120, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1790299, Length=107, Percent_Identity=31.7757009345794, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25493; Mature: 25493

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTVLIIEDEKDLAELLSFNLEREGYKTVIALDGAEGLEAVERVQPDLILLDLMLPGMFG
CCEEEEEECCHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEEEHHCCCCCC
TDVCKTVKKSEKSAHIPVVMLTAKGEEIDKVVGFEVGADDYVIKPFSTRELMLRVKAVLR
HHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHCEEECCCCEEECCCCHHHHHHHHHHHHH
RKAPEPAATNIINVGGIRIDTERHEVTVNGDEINFTTTEFKLLYTLVMRFGRVQSRDVLL
HCCCCCHHCCEEEECCEEEECCCEEEEECCCEEEEEHHHHHHHHHHHHHHCCCCCCCHHH
RDVWGYNFVEDTRTVDTHITRLRTKMGNAGDMIKTVRGFGYKLEVQ
HHHCCCCHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MQTVLIIEDEKDLAELLSFNLEREGYKTVIALDGAEGLEAVERVQPDLILLDLMLPGMFG
CCEEEEEECCHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEEEHHCCCCCC
TDVCKTVKKSEKSAHIPVVMLTAKGEEIDKVVGFEVGADDYVIKPFSTRELMLRVKAVLR
HHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHCEEECCCCEEECCCCHHHHHHHHHHHHH
RKAPEPAATNIINVGGIRIDTERHEVTVNGDEINFTTTEFKLLYTLVMRFGRVQSRDVLL
HCCCCCHHCCEEEECCEEEECCCEEEEECCCEEEEEHHHHHHHHHHHHHHCCCCCCCHHH
RDVWGYNFVEDTRTVDTHITRLRTKMGNAGDMIKTVRGFGYKLEVQ
HHHCCCCHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA