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The map label for this gene is fdnI-2 [H]

Identifier: 197117960

GI number: 197117960

Start: 1822038

End: 1822652

Strand: Reverse

Name: fdnI-2 [H]

Synonym: Gbem_1573

Alternate gene names: 197117960

Gene position: 1822652-1822038 (Counterclockwise)

Preceding gene: 197117961

Following gene: 197117959

Centisome position: 39.49

GC content: 60.33

Gene sequence:

>615_bases
ATGAGTAACTACATCGAGCGCTTCAGCACGCGGCAGCGCGTGCTGCATTGGATCGTGACCGCGAGCTTCTTCACCCTGGT
CCTCTCCGGGGTGGGGCTCTTCTCGCGCCTGTTCAACGGCTACTTCAACTTCTTCGGCGGTGGGCAGAACGCGATCCTCA
TCCACAAGATCGCCGGCCCCATCTTCTTCCTGAGTTCGGCCTATATGTACCTGGACCACAGGGCGGAGATCTCCAGCTTC
GACAGCAACGACATGGAGTGGTTCAGGAAGGCGGGGGGCTACCTCTCACGCGAGCCGCATCATTTCCACATCGGCAGGTA
CAACCCGGGGCAGAAGATGTTCGCCCTGTTCATAGCGGTAGCCACCCTGGTATTGGGGATCACCGGCCTCTTCATCTGGG
TCCCCACAGCTTTCCCGCGCTGGATCGTGCAGATCTCGCTGATGCTGCACGGGCTGATGTTCGTGGGTTCCATCATGTTC
ATGGTGCTGCACGTCTACCTGGCCACCATCGGCAACCCGGGGACGGTACAGGGGATGCTCTACGGAGACGTCCGCAAGGA
TTGGGCGCGTCATCACCATCCGAGATGGTACCGGGAGCGGACCGGCGGGAAATAA

Upstream 100 bases:

>100_bases
GGCATCCTCGGTTTCTGGGGGAGCATCGGCGCGGTGCTGCTGCACTACGTGACGGTCGGCCCGCAGCGCACCGAGGAGAC
GGAAGAGAAGGGAGGAAGTC

Downstream 100 bases:

>100_bases
ACCTTGAGACCACACAGACAGGAGCCGGAAATGTACAGCACGGAGAGGAAAATCGAGGCGCTGCAGAGCGCGGTGCACCG
GGCGCCGGAATATGCGGCCA

Product: formate dehydrogenase b-type cytochrome subunit

Products: CO2; NADH

Alternate protein names: Anaerobic formate dehydrogenase cytochrome b556 subunit; Formate dehydrogenase-N subunit gamma; FDH-N subunit gamma [H]

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MSNYIERFSTRQRVLHWIVTASFFTLVLSGVGLFSRLFNGYFNFFGGGQNAILIHKIAGPIFFLSSAYMYLDHRAEISSF
DSNDMEWFRKAGGYLSREPHHFHIGRYNPGQKMFALFIAVATLVLGITGLFIWVPTAFPRWIVQISLMLHGLMFVGSIMF
MVLHVYLATIGNPGTVQGMLYGDVRKDWARHHHPRWYRERTGGK

Sequences:

>Translated_204_residues
MSNYIERFSTRQRVLHWIVTASFFTLVLSGVGLFSRLFNGYFNFFGGGQNAILIHKIAGPIFFLSSAYMYLDHRAEISSF
DSNDMEWFRKAGGYLSREPHHFHIGRYNPGQKMFALFIAVATLVLGITGLFIWVPTAFPRWIVQISLMLHGLMFVGSIMF
MVLHVYLATIGNPGTVQGMLYGDVRKDWARHHHPRWYRERTGGK
>Mature_203_residues
SNYIERFSTRQRVLHWIVTASFFTLVLSGVGLFSRLFNGYFNFFGGGQNAILIHKIAGPIFFLSSAYMYLDHRAEISSFD
SNDMEWFRKAGGYLSREPHHFHIGRYNPGQKMFALFIAVATLVLGITGLFIWVPTAFPRWIVQISLMLHGLMFVGSIMFM
VLHVYLATIGNPGTVQGMLYGDVRKDWARHHHPRWYRERTGGK

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. Subunit gamma is the cytochrome b556(FDN) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787750, Length=202, Percent_Identity=32.1782178217822, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790325, Length=200, Percent_Identity=30.5, Blast_Score=95, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 23487; Mature: 23356

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNYIERFSTRQRVLHWIVTASFFTLVLSGVGLFSRLFNGYFNFFGGGQNAILIHKIAGP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHH
IFFLSSAYMYLDHRAEISSFDSNDMEWFRKAGGYLSREPHHFHIGRYNPGQKMFALFIAV
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHH
ATLVLGITGLFIWVPTAFPRWIVQISLMLHGLMFVGSIMFMVLHVYLATIGNPGTVQGML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHH
YGDVRKDWARHHHPRWYRERTGGK
HHHHHHHHHHHCCCHHHHHCCCCC
>Mature Secondary Structure 
SNYIERFSTRQRVLHWIVTASFFTLVLSGVGLFSRLFNGYFNFFGGGQNAILIHKIAGP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHH
IFFLSSAYMYLDHRAEISSFDSNDMEWFRKAGGYLSREPHHFHIGRYNPGQKMFALFIAV
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHH
ATLVLGITGLFIWVPTAFPRWIVQISLMLHGLMFVGSIMFMVLHVYLATIGNPGTVQGML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHH
YGDVRKDWARHHHPRWYRERTGGK
HHHHHHHHHHHCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]