Click here to switch to the map view.

The map label for this gene is fdnH-2 [H]

Identifier: 197117961

GI number: 197117961

Start: 1822645

End: 1823433

Strand: Reverse

Name: fdnH-2 [H]

Synonym: Gbem_1574

Alternate gene names: 197117961

Gene position: 1823433-1822645 (Counterclockwise)

Preceding gene: 308535250

Following gene: 197117960

Centisome position: 39.51

GC content: 61.72

Gene sequence:

>789_bases
ATGGCCGACACTATTAATCAAAGCGGCCGTAAAGGCTTTCTTGTCGATACTTCCAAGTGCATCGGCTGCCGCTCCTGCCA
GGTGGCGTGCAAGCAGTGGAACAAACTTGAGGCGGACAAAACCGCCAACATGGGGAGCACCGAGAACCCGCGCGACCTGA
CCCCGGCGCTCTACAACCGCATCCGGTTCATCGAGCAGGAGAGTTCAGGCCAGACGGTATGGCAGTTTTTGAACCAGCGC
TGCATGCACTGCGGCGACGCGGGGTGCATCAAGGTCTGCCCCTCCCCGGGTGCCTTGTACCGGACCAAGGAAGGGAGCGT
CGTCTTCAACAAGGAGAAATGCATCGCCTGCAAGTACTGCGTCTCGGCCTGCCCCTTCAACATCCCGCGCTACGACAGCA
ACGACAAGGTCGCCAAGTGCCATCTCTGCTATGACCGGATCGCGGCCGGGATGTCCCCCGCCTGCGCCAAGGCGTGTCCC
ACCCAGGCGCTGCAGTTCGGCAACCGCAACGACCTGATCAAGAAGGCCACGGCCGCCAAGAAAAAGCTCTACGGGGTGGA
TGACCTCTTGGGGCTCGGGGTGGTCTACGCGCTGGAAGGGCCGCCCGAGCAGTACGGTTTGCCGGCGAAACCGGAGATAC
CCACCTCGATCTTCCTCTGGAAGGACGTCATCAAACCGCTGGGCATCCTCGGTTTCTGGGGGAGCATCGGCGCGGTGCTG
CTGCACTACGTGACGGTCGGCCCGCAGCGCACCGAGGAGACGGAAGAGAAGGGAGGAAGTCATGAGTAA

Upstream 100 bases:

>100_bases
AGACGCCGCCAACCTGCTGACCCCCACCGCGGGGGACGCCAATACGATGATTCCCGAGACCAAGGCGTTCATGGCCGGGA
TCGCGAGAAAGGGGTGAGCC

Downstream 100 bases:

>100_bases
CTACATCGAGCGCTTCAGCACGCGGCAGCGCGTGCTGCATTGGATCGTGACCGCGAGCTTCTTCACCCTGGTCCTCTCCG
GGGTGGGGCTCTTCTCGCGC

Product: formate dehydrogenase iron-sulfur subunit

Products: CO2; NADH

Alternate protein names: Anaerobic formate dehydrogenase iron-sulfur subunit; Formate dehydrogenase-N subunit beta; FDH-N subunit beta [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MADTINQSGRKGFLVDTSKCIGCRSCQVACKQWNKLEADKTANMGSTENPRDLTPALYNRIRFIEQESSGQTVWQFLNQR
CMHCGDAGCIKVCPSPGALYRTKEGSVVFNKEKCIACKYCVSACPFNIPRYDSNDKVAKCHLCYDRIAAGMSPACAKACP
TQALQFGNRNDLIKKATAAKKKLYGVDDLLGLGVVYALEGPPEQYGLPAKPEIPTSIFLWKDVIKPLGILGFWGSIGAVL
LHYVTVGPQRTEETEEKGGSHE

Sequences:

>Translated_262_residues
MADTINQSGRKGFLVDTSKCIGCRSCQVACKQWNKLEADKTANMGSTENPRDLTPALYNRIRFIEQESSGQTVWQFLNQR
CMHCGDAGCIKVCPSPGALYRTKEGSVVFNKEKCIACKYCVSACPFNIPRYDSNDKVAKCHLCYDRIAAGMSPACAKACP
TQALQFGNRNDLIKKATAAKKKLYGVDDLLGLGVVYALEGPPEQYGLPAKPEIPTSIFLWKDVIKPLGILGFWGSIGAVL
LHYVTVGPQRTEETEEKGGSHE
>Mature_261_residues
ADTINQSGRKGFLVDTSKCIGCRSCQVACKQWNKLEADKTANMGSTENPRDLTPALYNRIRFIEQESSGQTVWQFLNQRC
MHCGDAGCIKVCPSPGALYRTKEGSVVFNKEKCIACKYCVSACPFNIPRYDSNDKVAKCHLCYDRIAAGMSPACAKACPT
QALQFGNRNDLIKKATAAKKKLYGVDDLLGLGVVYALEGPPEQYGLPAKPEIPTSIFLWKDVIKPLGILGFWGSIGAVLL
HYVTVGPQRTEETEEKGGSHE

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iro

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787749, Length=258, Percent_Identity=39.5348837209302, Blast_Score=214, Evalue=4e-57,
Organism=Escherichia coli, GI1790326, Length=256, Percent_Identity=41.015625, Blast_Score=210, Evalue=6e-56,
Organism=Escherichia coli, GI1787122, Length=177, Percent_Identity=38.4180790960452, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1787872, Length=177, Percent_Identity=36.7231638418079, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1789370, Length=190, Percent_Identity=33.1578947368421, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI2367345, Length=212, Percent_Identity=32.0754716981132, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI226510944, Length=181, Percent_Identity=34.2541436464088, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082179, Length=165, Percent_Identity=32.7272727272727, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI2367245, Length=159, Percent_Identity=36.4779874213836, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87082180, Length=173, Percent_Identity=29.4797687861272, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789067, Length=190, Percent_Identity=35.7894736842105, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788811, Length=162, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787478, Length=216, Percent_Identity=26.3888888888889, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787740, Length=128, Percent_Identity=29.6875, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1789079, Length=213, Percent_Identity=24.8826291079812, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1787963, Length=164, Percent_Identity=25, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 28724; Mature: 28593

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
6.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTINQSGRKGFLVDTSKCIGCRSCQVACKQWNKLEADKTANMGSTENPRDLTPALYNR
CCCCCCCCCCCCEEEECHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
IRFIEQESSGQTVWQFLNQRCMHCGDAGCIKVCPSPGALYRTKEGSVVFNKEKCIACKYC
HHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEECCCCEEECCCHHHHHHHH
VSACPFNIPRYDSNDKVAKCHLCYDRIAAGMSPACAKACPTQALQFGNRNDLIKKATAAK
HHHCCCCCCCCCCCCCEEHHHHHHHHHHCCCCHHHHHHCHHHHHHCCCHHHHHHHHHHHH
KKLYGVDDLLGLGVVYALEGPPEQYGLPAKPEIPTSIFLWKDVIKPLGILGFWGSIGAVL
HHHCCHHHHHHCCEEEEECCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LHYVTVGPQRTEETEEKGGSHE
HHHHHCCCCCCHHHHHCCCCCC
>Mature Secondary Structure 
ADTINQSGRKGFLVDTSKCIGCRSCQVACKQWNKLEADKTANMGSTENPRDLTPALYNR
CCCCCCCCCCCEEEECHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
IRFIEQESSGQTVWQFLNQRCMHCGDAGCIKVCPSPGALYRTKEGSVVFNKEKCIACKYC
HHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEECCCCEEECCCHHHHHHHH
VSACPFNIPRYDSNDKVAKCHLCYDRIAAGMSPACAKACPTQALQFGNRNDLIKKATAAK
HHHCCCCCCCCCCCCCEEHHHHHHHHHHCCCCHHHHHHCHHHHHHCCCHHHHHHHHHHHH
KKLYGVDDLLGLGVVYALEGPPEQYGLPAKPEIPTSIFLWKDVIKPLGILGFWGSIGAVL
HHHCCHHHHHHCCEEEEECCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LHYVTVGPQRTEETEEKGGSHE
HHHHHCCCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1834669; 9097039; 9278503; 11884747 [H]