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The map label for this gene is zraS [H]

Identifier: 197117813

GI number: 197117813

Start: 1643194

End: 1645155

Strand: Direct

Name: zraS [H]

Synonym: Gbem_1425

Alternate gene names: 197117813

Gene position: 1643194-1645155 (Clockwise)

Preceding gene: 197117812

Following gene: 197117814

Centisome position: 35.6

GC content: 62.23

Gene sequence:

>1962_bases
ATGATTGAGCTTGACCAAATTGGTGTAATATGGTACCACTATACCATTATTACGCCGGTCACCCGAAACGGTCTGGACAA
AGGTACCTTCCCCGCTGGGGGAGGAGCTCTGCAGGCGAGGTCGTCGACCTACCAGGAAAAGGATCGTTACATGACCGCCT
TTTTCCGCACATTCAAACTCCCGCTTGCCCTTTTGCTTATCGTAGGCCTGACCGCCTGCGCCTTCATCACCATCCGCCGC
GCCAACAACGGGGTCCGCGCCGAGGCGCGCGCCCGCTTCTTCGAACAGTACAACCGTCAGCAGTACCTCGTCGCCGAACT
CACAGCGCACTCCCTCGATGAAATGTTCGCCACCTTCAGCCGCAACCTCGAACTGGTCGCCGGCCTCTTCGACGGCAAAG
AGGTCGACCGCGAACAGTCGCAACGGCTCAAGGTGCATCTGGAAAAGATCTACGGCTCGCTGCTCGGTACCCCCGTTATC
GACCTGGTCGTCTTCGACAGCAAGGGGGTCGCCGTCGCCATAGAGCCGCACGACAATTTCACCTTGGGGCGCAGTTACGC
CTGGCGCGACTACTATCGGTGGGTGCGGGACCAGCGGCAGCCGGGGCGGATGTACATCTCGCCCTTCATGCAGTTGGCGG
GGGGGCGGAAACGCGGGGTAAAGGAGTTGATCGTAGCTAGAGGGATCTACGGCCCACGCGGCGAGTTCAACGGCGTGGTC
GCCTGCACGCTCGACTTCGACAAGCTGGCCCGAAAGCATGTCCTCTCCGTCAAGATCGGCAAGCACGGCCAGGCCTGGCT
CATGGACAGCACCACCAGGACCATCCTGGTCGACCCGAACGGCAGGATCGCGGGGCAGACCTTCGACGAGGCGCTGCGGC
GCAAATGGCCGCGCCTTTACGACCTGCTCCTTTCGGCTGAGGACGGCAAACCCGGCAGCGGCTGGTACGAGTTCGAGGAC
CCGGCCGACCCGCGCCTGAAGGTCCGCAAGCTGGTCAGCTACCACCCGGTGCGCCTGGAAAACCGGCTCTGGACAGTGGG
GGTGACCACGCCGGAAAGGGAGGTCGAAGCGCTCCTCTCTTCGTTCCTGCAGCGGCAGGAGGCCATCTCCACCACGCTCC
TCGTCACCATAATCGGAGGAGCGGCATTCCTCCTCGCGCTGCTTTATCACTGGAACCGCACGCTTACCGCACAGGTCGGC
CTGCACACCCGCGCCTTGAGCGAGACACATTCGCGCCTGGAGGCAACCTTCGACGAATTGCTCGTCGCCAAGAAGGTGGC
CGCGGTCGGGCATCTGGCGCTCGGGCTCGCCCACGAGATCCGCAACCCCCTTTCCGCCATCCAGATGAACATGCAGATGA
TCCGCAAGAAGATCGAGCCCACCGGGGTCCTCTGCGAGAACTTCTCCATTGCCGACGGCGAAATCCAGCGCCTGAACCGC
CTGTTGAAAGACGTTCTGGATTTCGCGCGCCCACGCCCCCTGCGGCTGCAGACGGTCGATCTGGCCGAAATAGTGCGGCG
CCTGCTGCAACTGGTTGACCAACGCCTGGCCCAGTACGGCGTTACCCCCACCACCGATATCGAATCGCCGCTTGAGCTTG
TCTGCGACCCCGAGCAGATCCACCAGGTGCTCTTGAATCTGGTGCTGAACGCCATGGAGGCGATGGATGGGGTCGCCGGT
GACAGGACCCTCAAGGTCATCGCCTACCGCAAAGAGAGCCAGGCCTACGTACTTGTGAGCGATTCCGGGAGGGGGATAGG
CCCCGACAAGTGCGAGCAGCTGTTCGATCCCTTTTACACAACCAAGGTTTCAGGGGGAGGGCTTGGGTTGTCCATCCTGC
AGACCATCGTCCTCTCTCATGGCGGGTCCGTTTCCGTGACGGGCGGGACCGGCGCCGGCGCTACCTTTACCGTCGTACTT
CCCCTCGCGGGGCCTTCAGGAACTGGAGAAAAAACACCGTGA

Upstream 100 bases:

>100_bases
TCAAGGGAGGGGGAATTCGGAAACAGTTTGTAGGGGGGTGGGGGGAGGTAGAGGGAAGGGGATCTAATCGTCGCGGCGCG
GCCCCTAGAGGGACAAGGGC

Downstream 100 bases:

>100_bases
ACACCATTTTGATAGCTGACGATGACATCGCCATCCGGCGCACGCTGGAGCTGCACCTGACCGAAGAAGGTTATTCGGTG
GTGACGGCCGCGAACGGTCC

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 653; Mature: 653

Protein sequence:

>653_residues
MIELDQIGVIWYHYTIITPVTRNGLDKGTFPAGGGALQARSSTYQEKDRYMTAFFRTFKLPLALLLIVGLTACAFITIRR
ANNGVRAEARARFFEQYNRQQYLVAELTAHSLDEMFATFSRNLELVAGLFDGKEVDREQSQRLKVHLEKIYGSLLGTPVI
DLVVFDSKGVAVAIEPHDNFTLGRSYAWRDYYRWVRDQRQPGRMYISPFMQLAGGRKRGVKELIVARGIYGPRGEFNGVV
ACTLDFDKLARKHVLSVKIGKHGQAWLMDSTTRTILVDPNGRIAGQTFDEALRRKWPRLYDLLLSAEDGKPGSGWYEFED
PADPRLKVRKLVSYHPVRLENRLWTVGVTTPEREVEALLSSFLQRQEAISTTLLVTIIGGAAFLLALLYHWNRTLTAQVG
LHTRALSETHSRLEATFDELLVAKKVAAVGHLALGLAHEIRNPLSAIQMNMQMIRKKIEPTGVLCENFSIADGEIQRLNR
LLKDVLDFARPRPLRLQTVDLAEIVRRLLQLVDQRLAQYGVTPTTDIESPLELVCDPEQIHQVLLNLVLNAMEAMDGVAG
DRTLKVIAYRKESQAYVLVSDSGRGIGPDKCEQLFDPFYTTKVSGGGLGLSILQTIVLSHGGSVSVTGGTGAGATFTVVL
PLAGPSGTGEKTP

Sequences:

>Translated_653_residues
MIELDQIGVIWYHYTIITPVTRNGLDKGTFPAGGGALQARSSTYQEKDRYMTAFFRTFKLPLALLLIVGLTACAFITIRR
ANNGVRAEARARFFEQYNRQQYLVAELTAHSLDEMFATFSRNLELVAGLFDGKEVDREQSQRLKVHLEKIYGSLLGTPVI
DLVVFDSKGVAVAIEPHDNFTLGRSYAWRDYYRWVRDQRQPGRMYISPFMQLAGGRKRGVKELIVARGIYGPRGEFNGVV
ACTLDFDKLARKHVLSVKIGKHGQAWLMDSTTRTILVDPNGRIAGQTFDEALRRKWPRLYDLLLSAEDGKPGSGWYEFED
PADPRLKVRKLVSYHPVRLENRLWTVGVTTPEREVEALLSSFLQRQEAISTTLLVTIIGGAAFLLALLYHWNRTLTAQVG
LHTRALSETHSRLEATFDELLVAKKVAAVGHLALGLAHEIRNPLSAIQMNMQMIRKKIEPTGVLCENFSIADGEIQRLNR
LLKDVLDFARPRPLRLQTVDLAEIVRRLLQLVDQRLAQYGVTPTTDIESPLELVCDPEQIHQVLLNLVLNAMEAMDGVAG
DRTLKVIAYRKESQAYVLVSDSGRGIGPDKCEQLFDPFYTTKVSGGGLGLSILQTIVLSHGGSVSVTGGTGAGATFTVVL
PLAGPSGTGEKTP
>Mature_653_residues
MIELDQIGVIWYHYTIITPVTRNGLDKGTFPAGGGALQARSSTYQEKDRYMTAFFRTFKLPLALLLIVGLTACAFITIRR
ANNGVRAEARARFFEQYNRQQYLVAELTAHSLDEMFATFSRNLELVAGLFDGKEVDREQSQRLKVHLEKIYGSLLGTPVI
DLVVFDSKGVAVAIEPHDNFTLGRSYAWRDYYRWVRDQRQPGRMYISPFMQLAGGRKRGVKELIVARGIYGPRGEFNGVV
ACTLDFDKLARKHVLSVKIGKHGQAWLMDSTTRTILVDPNGRIAGQTFDEALRRKWPRLYDLLLSAEDGKPGSGWYEFED
PADPRLKVRKLVSYHPVRLENRLWTVGVTTPEREVEALLSSFLQRQEAISTTLLVTIIGGAAFLLALLYHWNRTLTAQVG
LHTRALSETHSRLEATFDELLVAKKVAAVGHLALGLAHEIRNPLSAIQMNMQMIRKKIEPTGVLCENFSIADGEIQRLNR
LLKDVLDFARPRPLRLQTVDLAEIVRRLLQLVDQRLAQYGVTPTTDIESPLELVCDPEQIHQVLLNLVLNAMEAMDGVAG
DRTLKVIAYRKESQAYVLVSDSGRGIGPDKCEQLFDPFYTTKVSGGGLGLSILQTIVLSHGGSVSVTGGTGAGATFTVVL
PLAGPSGTGEKTP

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=229, Percent_Identity=38.4279475982533, Blast_Score=150, Evalue=4e-37,
Organism=Escherichia coli, GI1788549, Length=227, Percent_Identity=36.1233480176211, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1790300, Length=234, Percent_Identity=33.7606837606838, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI145693157, Length=252, Percent_Identity=27.3809523809524, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1786600, Length=267, Percent_Identity=25.4681647940075, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789808, Length=218, Percent_Identity=25.6880733944954, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790346, Length=240, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI87081816, Length=272, Percent_Identity=28.6764705882353, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787894, Length=256, Percent_Identity=27.734375, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1788393, Length=220, Percent_Identity=25.4545454545455, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI48994928, Length=230, Percent_Identity=26.9565217391304, Blast_Score=66, Evalue=7e-12,
Organism=Escherichia coli, GI1786783, Length=226, Percent_Identity=25.6637168141593, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786912, Length=221, Percent_Identity=27.1493212669683, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788713, Length=232, Percent_Identity=24.5689655172414, Blast_Score=64, Evalue=4e-11,
Organism=Escherichia coli, GI1789149, Length=274, Percent_Identity=26.2773722627737, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 72660; Mature: 72660

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELDQIGVIWYHYTIITPVTRNGLDKGTFPAGGGALQARSSTYQEKDRYMTAFFRTFKL
CCEEHHEEEEEEEEEEEEEHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
PLALLLIVGLTACAFITIRRANNGVRAEARARFFEQYNRQQYLVAELTAHSLDEMFATFS
HHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
RNLELVAGLFDGKEVDREQSQRLKVHLEKIYGSLLGTPVIDLVVFDSKGVAVAIEPHDNF
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCCC
TLGRSYAWRDYYRWVRDQRQPGRMYISPFMQLAGGRKRGVKELIVARGIYGPRGEFNGVV
CCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHCCHHHHHHHHCCCCCCCCCCCEE
ACTLDFDKLARKHVLSVKIGKHGQAWLMDSTTRTILVDPNGRIAGQTFDEALRRKWPRLY
EEEECHHHHHHHHEEEEEECCCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHCHHHH
DLLLSAEDGKPGSGWYEFEDPADPRLKVRKLVSYHPVRLENRLWTVGVTTPEREVEALLS
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCEEEEEECCCCHHHHHHHHH
SFLQRQEAISTTLLVTIIGGAAFLLALLYHWNRTLTAQVGLHTRALSETHSRLEATFDEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
LVAKKVAAVGHLALGLAHEIRNPLSAIQMNMQMIRKKIEPTGVLCENFSIADGEIQRLNR
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHH
LLKDVLDFARPRPLRLQTVDLAEIVRRLLQLVDQRLAQYGVTPTTDIESPLELVCDPEQI
HHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHH
HQVLLNLVLNAMEAMDGVAGDRTLKVIAYRKESQAYVLVSDSGRGIGPDKCEQLFDPFYT
HHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHCCHHE
TKVSGGGLGLSILQTIVLSHGGSVSVTGGTGAGATFTVVLPLAGPSGTGEKTP
EEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MIELDQIGVIWYHYTIITPVTRNGLDKGTFPAGGGALQARSSTYQEKDRYMTAFFRTFKL
CCEEHHEEEEEEEEEEEEEHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
PLALLLIVGLTACAFITIRRANNGVRAEARARFFEQYNRQQYLVAELTAHSLDEMFATFS
HHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
RNLELVAGLFDGKEVDREQSQRLKVHLEKIYGSLLGTPVIDLVVFDSKGVAVAIEPHDNF
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCCC
TLGRSYAWRDYYRWVRDQRQPGRMYISPFMQLAGGRKRGVKELIVARGIYGPRGEFNGVV
CCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHCCHHHHHHHHCCCCCCCCCCCEE
ACTLDFDKLARKHVLSVKIGKHGQAWLMDSTTRTILVDPNGRIAGQTFDEALRRKWPRLY
EEEECHHHHHHHHEEEEEECCCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHCHHHH
DLLLSAEDGKPGSGWYEFEDPADPRLKVRKLVSYHPVRLENRLWTVGVTTPEREVEALLS
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCEEEEEECCCCHHHHHHHHH
SFLQRQEAISTTLLVTIIGGAAFLLALLYHWNRTLTAQVGLHTRALSETHSRLEATFDEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
LVAKKVAAVGHLALGLAHEIRNPLSAIQMNMQMIRKKIEPTGVLCENFSIADGEIQRLNR
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHH
LLKDVLDFARPRPLRLQTVDLAEIVRRLLQLVDQRLAQYGVTPTTDIESPLELVCDPEQI
HHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHH
HQVLLNLVLNAMEAMDGVAGDRTLKVIAYRKESQAYVLVSDSGRGIGPDKCEQLFDPFYT
HHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHCCHHE
TKVSGGGLGLSILQTIVLSHGGSVSVTGGTGAGATFTVVLPLAGPSGTGEKTP
EEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]