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The map label for this gene is metK-1

Identifier: 197117729

GI number: 197117729

Start: 1545994

End: 1547163

Strand: Direct

Name: metK-1

Synonym: Gbem_1341

Alternate gene names: 197117729

Gene position: 1545994-1547163 (Clockwise)

Preceding gene: 197117727

Following gene: 197117732

Centisome position: 33.5

GC content: 59.66

Gene sequence:

>1170_bases
ATGGAAATGAAGGATTTTATTTTTACCTCCGAGTCTGTCTCTGAAGGTCATCCGGATAAGGTTGCGGACCAAGTATCAGA
CGCCATTTTGGATGCCATCCTGACCCAGGACCCCAAGTCGCGGGTAGCCTGTGAAACGATGGTAACGACAGGCATGGCGG
TCATCGCCGGTGAGATCACCACCAACGCCATCATCGACTATCCCAAGATCGTCCGCGAGACCATCCGCGAGATCGGCTAC
AACGACTCCGCCATGGGTTTCGATTGGGAAACTTGCGCTGTTCTTACCTCCATCGACAAGCAGTCCCCGGACATCGCTCA
GGGTGTTACCGAGGGCGAGGGGATGTTCAAGGAGCAGGGGGCGGGGGACCAGGGCCTCATGTTCGGTTTCGCCTGCAACG
AGACGCCTGAACTGATGCCGATGTCGATTCTGTTGGCTCACAAGCTGGTGTCGCGTCTGGCCGACGTTCGCAAGGCCGGC
GTGCTCGACTTCCTGCGTCCCGACTCCAAGTCCCAGGTTTCCATCCAGTACATCGACGACAAGCCGGTGCATGTGGATAC
CGTTGTCATTTCCTCGCAGCACTCCCCTGAGGTCTCCTACGAGATGATCAAGGAAGGGATCATCGAGGAAGTCGTGAAGA
AGATTATCCCGGCCAACCTGATGGACTCCAAGACCAAGTTCCTGATCAACCCGACCGGACGTTTCGTCATCGGCGGCCCG
ATGGGCGACTGCGGCCTTACCGGCCGTAAGATCATCGTTGACAGCTACGGCGGGCACGGCGCTCACGGCGGCGGCGCGTT
CTCCGGCAAGGACCCCAGCAAGGTTGACCGTTCCGCCGCGTACATGGGGCGTTACGTGGCGAAGAACCTGGTGGCATCGG
GTGTCTGCGAAAGGTGCGAGGTTCAGGTGGCTTACGCCATCGGCGTCGCCGAACCGGTCTCCGTCATGGTCGACTGCAAC
GGCACCGGGAAGATTCCGTCCAAGCGCATCTCCGAGATCGTGCGCGAGGTATTCGACATGCGTCCCCGCGCCATCATCGA
GCAGCTCGACCTGCTGCGTCCGATCTACAGGAAGACCGCTGCCTACGGCCACTTCGGCCGCGAGCTTCCCGAGTTCACCT
GGGAGCGCACTGACAAGGCTGCCATCATCAGGGAAAAAGCCGGGCTGTAA

Upstream 100 bases:

>100_bases
AAATTGGTCCCATTTAATTATTGACAAAGAGAATTGGCAACTATACAATTCCCCAGCTTTAGGCTACCTGCTGCACACTT
CGTAAGGGGGTTTTTACCGT

Downstream 100 bases:

>100_bases
GAACCGGTTCAACGTTCAACATTCTACGTAAAACAAAAAAGGCCCTCTGGAATTCCGGGGGGCCTTTCTTTATAACCCGT
TCAACGTTCAACGTTCCGAC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MEMKDFIFTSESVSEGHPDKVADQVSDAILDAILTQDPKSRVACETMVTTGMAVIAGEITTNAIIDYPKIVRETIREIGY
NDSAMGFDWETCAVLTSIDKQSPDIAQGVTEGEGMFKEQGAGDQGLMFGFACNETPELMPMSILLAHKLVSRLADVRKAG
VLDFLRPDSKSQVSIQYIDDKPVHVDTVVISSQHSPEVSYEMIKEGIIEEVVKKIIPANLMDSKTKFLINPTGRFVIGGP
MGDCGLTGRKIIVDSYGGHGAHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVASGVCERCEVQVAYAIGVAEPVSVMVDCN
GTGKIPSKRISEIVREVFDMRPRAIIEQLDLLRPIYRKTAAYGHFGRELPEFTWERTDKAAIIREKAGL

Sequences:

>Translated_389_residues
MEMKDFIFTSESVSEGHPDKVADQVSDAILDAILTQDPKSRVACETMVTTGMAVIAGEITTNAIIDYPKIVRETIREIGY
NDSAMGFDWETCAVLTSIDKQSPDIAQGVTEGEGMFKEQGAGDQGLMFGFACNETPELMPMSILLAHKLVSRLADVRKAG
VLDFLRPDSKSQVSIQYIDDKPVHVDTVVISSQHSPEVSYEMIKEGIIEEVVKKIIPANLMDSKTKFLINPTGRFVIGGP
MGDCGLTGRKIIVDSYGGHGAHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVASGVCERCEVQVAYAIGVAEPVSVMVDCN
GTGKIPSKRISEIVREVFDMRPRAIIEQLDLLRPIYRKTAAYGHFGRELPEFTWERTDKAAIIREKAGL
>Mature_389_residues
MEMKDFIFTSESVSEGHPDKVADQVSDAILDAILTQDPKSRVACETMVTTGMAVIAGEITTNAIIDYPKIVRETIREIGY
NDSAMGFDWETCAVLTSIDKQSPDIAQGVTEGEGMFKEQGAGDQGLMFGFACNETPELMPMSILLAHKLVSRLADVRKAG
VLDFLRPDSKSQVSIQYIDDKPVHVDTVVISSQHSPEVSYEMIKEGIIEEVVKKIIPANLMDSKTKFLINPTGRFVIGGP
MGDCGLTGRKIIVDSYGGHGAHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVASGVCERCEVQVAYAIGVAEPVSVMVDCN
GTGKIPSKRISEIVREVFDMRPRAIIEQLDLLRPIYRKTAAYGHFGRELPEFTWERTDKAAIIREKAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=379, Percent_Identity=56.2005277044855, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI4557737, Length=374, Percent_Identity=56.1497326203209, Blast_Score=427, Evalue=1e-120,
Organism=Escherichia coli, GI1789311, Length=387, Percent_Identity=64.8578811369509, Blast_Score=528, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI17538494, Length=380, Percent_Identity=57.1052631578947, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17538498, Length=380, Percent_Identity=56.8421052631579, Blast_Score=430, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI25145633, Length=389, Percent_Identity=55.5269922879177, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17551082, Length=378, Percent_Identity=55.2910052910053, Blast_Score=417, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI32565905, Length=380, Percent_Identity=51.5789473684211, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32565911, Length=329, Percent_Identity=55.3191489361702, Blast_Score=357, Evalue=7e-99,
Organism=Caenorhabditis elegans, GI25145635, Length=338, Percent_Identity=53.8461538461538, Blast_Score=355, Evalue=3e-98,
Organism=Caenorhabditis elegans, GI32565903, Length=302, Percent_Identity=55.9602649006622, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI32565909, Length=86, Percent_Identity=59.3023255813954, Blast_Score=117, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=68.1818181818182, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320710, Length=380, Percent_Identity=57.6315789473684, Blast_Score=442, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6323209, Length=375, Percent_Identity=57.8666666666667, Blast_Score=441, Evalue=1e-125,
Organism=Drosophila melanogaster, GI45552159, Length=374, Percent_Identity=56.1497326203209, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580529, Length=374, Percent_Identity=56.1497326203209, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580537, Length=374, Percent_Identity=56.1497326203209, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580533, Length=374, Percent_Identity=56.1497326203209, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580535, Length=374, Percent_Identity=56.1497326203209, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580531, Length=374, Percent_Identity=56.1497326203209, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580527, Length=374, Percent_Identity=55.6149732620321, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580525, Length=374, Percent_Identity=55.6149732620321, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580541, Length=361, Percent_Identity=54.5706371191136, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580539, Length=122, Percent_Identity=58.1967213114754, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_GEOSM (C6E2L2)

Other databases:

- EMBL:   CP001661
- RefSeq:   YP_003022730.1
- GeneID:   8138286
- GenomeReviews:   CP001661_GR
- KEGG:   gem:GM21_2943
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42432; Mature: 42432

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMKDFIFTSESVSEGHPDKVADQVSDAILDAILTQDPKSRVACETMVTTGMAVIAGEIT
CCCHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHEEHHHH
TNAIIDYPKIVRETIREIGYNDSAMGFDWETCAVLTSIDKQSPDIAQGVTEGEGMFKEQG
HHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHCC
AGDQGLMFGFACNETPELMPMSILLAHKLVSRLADVRKAGVLDFLRPDSKSQVSIQYIDD
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEEEEECC
KPVHVDTVVISSQHSPEVSYEMIKEGIIEEVVKKIIPANLMDSKTKFLINPTGRFVIGGP
CCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEECCC
MGDCGLTGRKIIVDSYGGHGAHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVASGVCERCE
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQVAYAIGVAEPVSVMVDCNGTGKIPSKRISEIVREVFDMRPRAIIEQLDLLRPIYRKTA
EEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AYGHFGRELPEFTWERTDKAAIIREKAGL
HHHHHHHHHHHHHHHCCCHHHHHHHHCCC
>Mature Secondary Structure
MEMKDFIFTSESVSEGHPDKVADQVSDAILDAILTQDPKSRVACETMVTTGMAVIAGEIT
CCCHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHEEHHHH
TNAIIDYPKIVRETIREIGYNDSAMGFDWETCAVLTSIDKQSPDIAQGVTEGEGMFKEQG
HHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHCC
AGDQGLMFGFACNETPELMPMSILLAHKLVSRLADVRKAGVLDFLRPDSKSQVSIQYIDD
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEEEEECC
KPVHVDTVVISSQHSPEVSYEMIKEGIIEEVVKKIIPANLMDSKTKFLINPTGRFVIGGP
CCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEECCC
MGDCGLTGRKIIVDSYGGHGAHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVASGVCERCE
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQVAYAIGVAEPVSVMVDCNGTGKIPSKRISEIVREVFDMRPRAIIEQLDLLRPIYRKTA
EEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AYGHFGRELPEFTWERTDKAAIIREKAGL
HHHHHHHHHHHHHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA