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The map label for this gene is pcrA [H]

Identifier: 197117725

GI number: 197117725

Start: 1541534

End: 1543570

Strand: Direct

Name: pcrA [H]

Synonym: Gbem_1337

Alternate gene names: 197117725

Gene position: 1541534-1543570 (Clockwise)

Preceding gene: 197117723

Following gene: 197117727

Centisome position: 33.4

GC content: 61.27

Gene sequence:

>2037_bases
ATGCTCGACCTGTCACGATTGAACCCCGAACAGCTGGCCGCCGTAAAGCACACGGAGGGGGCCCTGCTGGTCCTCGCCGG
CGCCGGCTCCGGCAAGACCGGGGTCATCACCTACCGTATCGCGCACCTCATCCTGGACAAGAAGGTGCCGCCCGACCAGG
TCCTCGCCGTCACCTTCACCAACAAGGCCGCCAAGGAGATGAAGGAGCGGGTGGAGCACCTGGTGGGGCGCAAGCAGTCG
AAGGGAATCGTTCTTTCCACCTTCCATTCGCTGGGCGTGCGCGTGCTGAAACGGGACATCGAGCGCCTGGGCTACAAGAA
AAACTTCTCCATCTATTCCACCGCGGACCAGGTGGGGCTCGTGCGCCAGATCGTCCGCGAGGTGAATACCGACAGCAAGA
AGTACGACGCCGAAAGCATCATCTGGCGCATATCGGGCGCGAAGAACAAGCTGATCCCGCCGGGGAGGTTCACCCCCAAC
CCGACGGATGACATCGACATGATGGCGGGGCTCGTCTATCCGCGCTACCAGTCAGCCTTAAAGGCGTTTAACGCCATTGA
TTTCGACGACATCATCATGCTGACGGCGGAGCTCCTCGAGCATCACCCTCTGGTCCTCAAACACTGGCAGGAGCGCTTTC
GCTACATCATGGTCGACGAATACCAGGACACGAACTCCTCGCAGTACCTCCTGGTGAACCTCCTGGCCGCCGGGTGCGGC
AACCTCTGCGTCGTAGGCGACGACGACCAGTCCATCTACGGCTGGCGCGGCGCGGACGTCGGCAACATCCTCGACTTCGA
GAAGGATTTCAAGGGGTGCCGGACCATCAAGCTGGAGCAGAACTACCGCTCCACCGGCAACATCCTGGAAGCGGCCAACA
GCGTGATCGGCAACAACAAGGTGCGCAAGGCGAAGAGGCTTTGGACCGCTTCGGGGGAGGGGCCCCTCATCGATCTCTGC
ATCGTGCAGGACGACGAGGAGGAGGCGACCAGCGTCGTGGAGCGGATCCAGCTTGAGCGTTACAAAAAGGACGCCCCCTA
CAGCCAGTTCGCCATCCTTTATCGCACCAACGCGCAAAGTCGCCCCTTCGAGGAGCAGCTCCGCTTCGAGGACATACCGT
ACGTCCTGGTGGGGGGGACCCAGTTCTTCGAGCGTAAGGAGGTGAAGGACTCCCTCTCCTACCTGAAGGTGATCGCAAAT
CCGCTGGACGAGGTGGCGCTTTTGCGCATCGTCAACTTCCCCAAGCGCGGCATCGGCGACAGCACCGTGATCCGCATCAA
CCAGTGGTCGCTGGAAAAGGAGATCCCTCTTTTCGAGGCCTTCAGCCGGGTGGAGGAGATCGAGGGGATTTCCGACATCA
TCAAGGACAAGGTCCGCGCCTTCCACCACACGCTTTTGGACGCCGCCGAAGCGTTCCGGGCGGAAGGAGGGCTCGCCGAG
AAAGGGAAGGCCCTTTTCGAGAAACTGAAGATAGAAGAGGAGATCTTCCGCACCATAGACGACGCCAAGGCCGCCCGCCG
CAAGGTGGAGAACGTGGAGCAGATCATCAACTCGATGGCGGCCTACGAGGAGCGGGTGCCCGAGGCGACCCTGGGCGGGT
TCATCGAGAAGGTGTCGCTGATGGACGAGGACCGCTTCTCCGGCAAGGACAAGAAGGAACACGGCAAGGATGCGGTGACG
CTCATGTCGCTGCATTCCAGCAAGGGGCTGGAGTTTCCTTTCGTGTTCCTGGTCGGGATGGAAGACGAGATCCTGCCCCA
CAAGCGCGCCATCTACGAGGACGACAGCATCGACGAGGAGCGGCGCCTTTGCTACGTGGGGATCACCCGGGCGCGGCAGC
AGCTGGTGATGACCCGGACGCTCTTCCGGAAGAAGTATGGAAAGCTGGAGGAGCGCGTGCCGTCGCGTTTTCTCGAAGAG
ATCCCGGGGGGAGTGCTCAACGTGCAGCAAAGCGGCGTGGTGAAGGAAGTGACCCCCGAAGAGGCCGAGAAGAGCGCGGA
AGATTTCTTCGCCAAGATGAAGGCGATGATGGGGTAA

Upstream 100 bases:

>100_bases
TGTTGGGCCGCGCCTTCCCGGACGCGGCTCTTTTTTCGTCCGCGCCGCGGGGGAAACTGCCGCCTTGCCGGCGGAACTAC
CCGCTTTTGATGAGGTTTTC

Downstream 100 bases:

>100_bases
GGGCGGAAAACCGTCGGAGGCGAACCCCCCCTGTCCCCCCTTCGCAAAGGGGGGGACGCAATAGCCTCGTACATCTCAAA
GGGGGGGCACGAAAAAACTG

Product: UvrD superfamily ATP-dependent helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 678; Mature: 678

Protein sequence:

>678_residues
MLDLSRLNPEQLAAVKHTEGALLVLAGAGSGKTGVITYRIAHLILDKKVPPDQVLAVTFTNKAAKEMKERVEHLVGRKQS
KGIVLSTFHSLGVRVLKRDIERLGYKKNFSIYSTADQVGLVRQIVREVNTDSKKYDAESIIWRISGAKNKLIPPGRFTPN
PTDDIDMMAGLVYPRYQSALKAFNAIDFDDIIMLTAELLEHHPLVLKHWQERFRYIMVDEYQDTNSSQYLLVNLLAAGCG
NLCVVGDDDQSIYGWRGADVGNILDFEKDFKGCRTIKLEQNYRSTGNILEAANSVIGNNKVRKAKRLWTASGEGPLIDLC
IVQDDEEEATSVVERIQLERYKKDAPYSQFAILYRTNAQSRPFEEQLRFEDIPYVLVGGTQFFERKEVKDSLSYLKVIAN
PLDEVALLRIVNFPKRGIGDSTVIRINQWSLEKEIPLFEAFSRVEEIEGISDIIKDKVRAFHHTLLDAAEAFRAEGGLAE
KGKALFEKLKIEEEIFRTIDDAKAARRKVENVEQIINSMAAYEERVPEATLGGFIEKVSLMDEDRFSGKDKKEHGKDAVT
LMSLHSSKGLEFPFVFLVGMEDEILPHKRAIYEDDSIDEERRLCYVGITRARQQLVMTRTLFRKKYGKLEERVPSRFLEE
IPGGVLNVQQSGVVKEVTPEEAEKSAEDFFAKMKAMMG

Sequences:

>Translated_678_residues
MLDLSRLNPEQLAAVKHTEGALLVLAGAGSGKTGVITYRIAHLILDKKVPPDQVLAVTFTNKAAKEMKERVEHLVGRKQS
KGIVLSTFHSLGVRVLKRDIERLGYKKNFSIYSTADQVGLVRQIVREVNTDSKKYDAESIIWRISGAKNKLIPPGRFTPN
PTDDIDMMAGLVYPRYQSALKAFNAIDFDDIIMLTAELLEHHPLVLKHWQERFRYIMVDEYQDTNSSQYLLVNLLAAGCG
NLCVVGDDDQSIYGWRGADVGNILDFEKDFKGCRTIKLEQNYRSTGNILEAANSVIGNNKVRKAKRLWTASGEGPLIDLC
IVQDDEEEATSVVERIQLERYKKDAPYSQFAILYRTNAQSRPFEEQLRFEDIPYVLVGGTQFFERKEVKDSLSYLKVIAN
PLDEVALLRIVNFPKRGIGDSTVIRINQWSLEKEIPLFEAFSRVEEIEGISDIIKDKVRAFHHTLLDAAEAFRAEGGLAE
KGKALFEKLKIEEEIFRTIDDAKAARRKVENVEQIINSMAAYEERVPEATLGGFIEKVSLMDEDRFSGKDKKEHGKDAVT
LMSLHSSKGLEFPFVFLVGMEDEILPHKRAIYEDDSIDEERRLCYVGITRARQQLVMTRTLFRKKYGKLEERVPSRFLEE
IPGGVLNVQQSGVVKEVTPEEAEKSAEDFFAKMKAMMG
>Mature_678_residues
MLDLSRLNPEQLAAVKHTEGALLVLAGAGSGKTGVITYRIAHLILDKKVPPDQVLAVTFTNKAAKEMKERVEHLVGRKQS
KGIVLSTFHSLGVRVLKRDIERLGYKKNFSIYSTADQVGLVRQIVREVNTDSKKYDAESIIWRISGAKNKLIPPGRFTPN
PTDDIDMMAGLVYPRYQSALKAFNAIDFDDIIMLTAELLEHHPLVLKHWQERFRYIMVDEYQDTNSSQYLLVNLLAAGCG
NLCVVGDDDQSIYGWRGADVGNILDFEKDFKGCRTIKLEQNYRSTGNILEAANSVIGNNKVRKAKRLWTASGEGPLIDLC
IVQDDEEEATSVVERIQLERYKKDAPYSQFAILYRTNAQSRPFEEQLRFEDIPYVLVGGTQFFERKEVKDSLSYLKVIAN
PLDEVALLRIVNFPKRGIGDSTVIRINQWSLEKEIPLFEAFSRVEEIEGISDIIKDKVRAFHHTLLDAAEAFRAEGGLAE
KGKALFEKLKIEEEIFRTIDDAKAARRKVENVEQIINSMAAYEERVPEATLGGFIEKVSLMDEDRFSGKDKKEHGKDAVT
LMSLHSSKGLEFPFVFLVGMEDEILPHKRAIYEDDSIDEERRLCYVGITRARQQLVMTRTLFRKKYGKLEERVPSRFLEE
IPGGVLNVQQSGVVKEVTPEEAEKSAEDFFAKMKAMMG

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=681, Percent_Identity=41.9970631424376, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI2367296, Length=647, Percent_Identity=39.4126738794436, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1787196, Length=340, Percent_Identity=29.1176470588235, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6322369, Length=757, Percent_Identity=28.7978863936592, Blast_Score=202, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6324477, Length=681, Percent_Identity=22.9074889867841, Blast_Score=117, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 76856; Mature: 76856

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLSRLNPEQLAAVKHTEGALLVLAGAGSGKTGVITYRIAHLILDKKVPPDQVLAVTFT
CCCCCCCCHHHHHHHEECCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEC
NKAAKEMKERVEHLVGRKQSKGIVLSTFHSLGVRVLKRDIERLGYKKNFSIYSTADQVGL
CHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHH
VRQIVREVNTDSKKYDAESIIWRISGAKNKLIPPGRFTPNPTDDIDMMAGLVYPRYQSAL
HHHHHHHHCCCCCCCCCHHHEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHH
KAFNAIDFDDIIMLTAELLEHHPLVLKHWQERFRYIMVDEYQDTNSSQYLLVNLLAAGCG
HHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHCCCC
NLCVVGDDDQSIYGWRGADVGNILDFEKDFKGCRTIKLEQNYRSTGNILEAANSVIGNNK
CEEEECCCCCCCCCCCCCCCCCHHCHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCH
VRKAKRLWTASGEGPLIDLCIVQDDEEEATSVVERIQLERYKKDAPYSQFAILYRTNAQS
HHHHHHHHCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCC
RPFEEQLRFEDIPYVLVGGTQFFERKEVKDSLSYLKVIANPLDEVALLRIVNFPKRGIGD
CCHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCC
STVIRINQWSLEKEIPLFEAFSRVEEIEGISDIIKDKVRAFHHTLLDAAEAFRAEGGLAE
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KGKALFEKLKIEEEIFRTIDDAKAARRKVENVEQIINSMAAYEERVPEATLGGFIEKVSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
MDEDRFSGKDKKEHGKDAVTLMSLHSSKGLEFPFVFLVGMEDEILPHKRAIYEDDSIDEE
HCCCCCCCCCCHHHCCHHEEHEEHHCCCCCCCCEEEEECCCHHHCCCHHHHCCCCCCCHH
RRLCYVGITRARQQLVMTRTLFRKKYGKLEERVPSRFLEEIPGGVLNVQQSGVVKEVTPE
HCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHH
EAEKSAEDFFAKMKAMMG
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLDLSRLNPEQLAAVKHTEGALLVLAGAGSGKTGVITYRIAHLILDKKVPPDQVLAVTFT
CCCCCCCCHHHHHHHEECCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEC
NKAAKEMKERVEHLVGRKQSKGIVLSTFHSLGVRVLKRDIERLGYKKNFSIYSTADQVGL
CHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHH
VRQIVREVNTDSKKYDAESIIWRISGAKNKLIPPGRFTPNPTDDIDMMAGLVYPRYQSAL
HHHHHHHHCCCCCCCCCHHHEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHH
KAFNAIDFDDIIMLTAELLEHHPLVLKHWQERFRYIMVDEYQDTNSSQYLLVNLLAAGCG
HHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHCCCC
NLCVVGDDDQSIYGWRGADVGNILDFEKDFKGCRTIKLEQNYRSTGNILEAANSVIGNNK
CEEEECCCCCCCCCCCCCCCCCHHCHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCH
VRKAKRLWTASGEGPLIDLCIVQDDEEEATSVVERIQLERYKKDAPYSQFAILYRTNAQS
HHHHHHHHCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCC
RPFEEQLRFEDIPYVLVGGTQFFERKEVKDSLSYLKVIANPLDEVALLRIVNFPKRGIGD
CCHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCC
STVIRINQWSLEKEIPLFEAFSRVEEIEGISDIIKDKVRAFHHTLLDAAEAFRAEGGLAE
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KGKALFEKLKIEEEIFRTIDDAKAARRKVENVEQIINSMAAYEERVPEATLGGFIEKVSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
MDEDRFSGKDKKEHGKDAVTLMSLHSSKGLEFPFVFLVGMEDEILPHKRAIYEDDSIDEE
HCCCCCCCCCCHHHCCHHEEHEEHHCCCCCCCCEEEEECCCHHHCCCHHHHCCCCCCCHH
RRLCYVGITRARQQLVMTRTLFRKKYGKLEERVPSRFLEEIPGGVLNVQQSGVVKEVTPE
HCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHH
EAEKSAEDFFAKMKAMMG
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]