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The map label for this gene is ligA

Identifier: 197117724

GI number: 197117724

Start: 1539236

End: 1541242

Strand: Reverse

Name: ligA

Synonym: Gbem_1336

Alternate gene names: 197117724

Gene position: 1541242-1539236 (Counterclockwise)

Preceding gene: 197117726

Following gene: 197117717

Centisome position: 33.4

GC content: 63.38

Gene sequence:

>2007_bases
ATGGATAAAACTGCTGCGGCTCAAAGGATCAAGTGGTTAAGCTCGGAGATCGAGCGCCACAACCGGCTCTACTACGAACA
GGACATGCCGGAGATAACCGATGCCGAGTACGATGCGCTCTTCCGCGAGTTGAAGGAGCTTGAGGCCTCCTATCCCGACC
TCGCCCTCCCCGATTCCCCGACCGGCCGGGTCGGCGGGCGCCCGGTGGCGAAGTTCACCCAGGTGCGCCACACAACGCCC
ATGCTTTCGCTGGAAAACGCCTTCACGGAGAAGGACATCGTCGACTTCGATGACCGGATGAAGCGCTTCCTGGGGCTTTC
AGGCGCAGAGGAGATCGGTTATGTTTGCGAGCCGAAGATGGACGGCGTCGCGGTGGAGTTGGTGTACCGTGATGGGCTCC
TCGCCATCGGCTCTACCCGCGGCGACGGGTTGGTGGGCGAGGAGGTGACCCAGAACCTGAAGACCATCAAGGACATACCG
TTGCGGCTGCAGACGGAGGAGCCTCCGGGGCTCTTGACGGTGCGGGGTGAGGTGTACCTGCCGCTGGAACCTTTCAGGAA
GTTCAACCAGGAGCGCGAGGAAGCGGGGGAGCCACCCTTCGCCAACCCGAGGAACGCCGCGGCAGGATCCCTGCGCCAGC
TCGACTCGAAGATCACCGCAAAGCGGCCGCTCAGCATCTTCTGCTACGCCCCTGGCGAGGTGGACGGAGTCGCCTTCCAA
TCGCAAAGCGATTTCCTCAGCACCATCCCCACCTGGCGCATCCCGGTCAACCCGCTGACGAGGCTCGTCCCGGGCGTGCA
GGGGGTGCTCGACTACTACAACGAGATGATGGAAAAGCGCGACGATCTCCCCTACGAGATCGACGGCGTGGTAGTGAAGG
TGGATCGGTTTTCCCTGCAGCGCGACTTGGGAGAGAAGAGCCGCTCGCCGCGCTGGGCCATCGCCTGGAAGTTCCCCCCG
CGCCAGGCGACCACGGTGGTCAACGACATCGTGCCCCAGGTGGGTCGGACCGGCGTCATCACCCCTGTGGCGCACCTTGA
GCCGGTGAACGTCTCGGGCGTCATGGTCTCCCGCGCCACACTGCACAACTGGGAGGAGATGGAGCGCAAGGACATCCGCA
GGGGCGATACCGTAGTGGTCGAGCGCGCCGGCGACGTCATTCCCGCCGTGGTGCAGGTGCTGGTCGAGAAAAGGCAAGGG
AGCGAGACGCTGCTTCCGGTACCGCAGGCGTGTCCTGTGTGCGGCGGCGAAGTGGTCAGGATACCGGGCGAGGTCGCCGT
GCGCTGCGTCGGCCTCAACTGTCCCGCGCAGGCATTGGAACGGGTGAAGCACTTTGCCGCCCGCCGCGCCATGGACATAG
ACGGGCTGGGGGAGAAGTTCATCGAGCAGCTTCTGAACCTGAAGCTGATACGGAACGTCGCCGACATCTACCGGCTGACT
GAAGAGGACTTCATGCAGTTCGAGCGCATGGGGAAGAAGCTGGCGGAGAACCTTCTGAATTCCATCGCCGCCAGCAAGGA
GCGGGAGCTTTCGCGCCTGATCTTCGCGCTCGGGATACGGCACGTGGGGGAGCACACCGCGAAGCTTTTGGCCAGCGCCT
TCGGGAGCATGGAGAATCTCGCGGCGGCGAGCGAAGAGGAGTTGACCTCCATCCGCGAAGTCGGGCCGCAGGTGGCGGCT
AGCATCGCGGATTTCTTCAAGAGCGAAGAGAACCTGGAAGTGCTCAGGGAGTTAAAGGAGCACGGGGTTAATCCCAGGGT
GGAAGAGAAGCGGGTTGGCGGCAGGTTCACCGGCAAGACCTTCGTCTTCACCGGCGCCCTGGAGAAGTTCACGCGCGACG
AGGCGAAGAAGATGGTGGAGTTGGAGGGGGGGCATGCAGCCGGCTCCGTGTCCAAGAAGACCGACTACGTCGTCGCCGGC
GCCGATGCCGGGAGCAAGCTGGATAAAGCGCAGCAGCTGGGCGTGCGGGTGTTGAGCGAGGATGATTTTCTGGAGTTGAT
GCAGTAG

Upstream 100 bases:

>100_bases
AGCCCCCCCTCACCCGCCCCCTCCAAGTGGCGGGCGGTAAAGCAGGCCCGGGCTCCTGGCACCCAACAAAGACATTAAAG
ACCGAAAGAGGTTCAGGGAA

Downstream 100 bases:

>100_bases
GGATTCAGCGGTGCTCTTTACGGTAGTGGTAGAGCCTTTCGGTGAAGCCGCCGTTTTCGGCGAAGTCACTCGCCAGCCGG
TCCATCTGGCGCTTGAGCAG

Product: NAD-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 668; Mature: 668

Protein sequence:

>668_residues
MDKTAAAQRIKWLSSEIERHNRLYYEQDMPEITDAEYDALFRELKELEASYPDLALPDSPTGRVGGRPVAKFTQVRHTTP
MLSLENAFTEKDIVDFDDRMKRFLGLSGAEEIGYVCEPKMDGVAVELVYRDGLLAIGSTRGDGLVGEEVTQNLKTIKDIP
LRLQTEEPPGLLTVRGEVYLPLEPFRKFNQEREEAGEPPFANPRNAAAGSLRQLDSKITAKRPLSIFCYAPGEVDGVAFQ
SQSDFLSTIPTWRIPVNPLTRLVPGVQGVLDYYNEMMEKRDDLPYEIDGVVVKVDRFSLQRDLGEKSRSPRWAIAWKFPP
RQATTVVNDIVPQVGRTGVITPVAHLEPVNVSGVMVSRATLHNWEEMERKDIRRGDTVVVERAGDVIPAVVQVLVEKRQG
SETLLPVPQACPVCGGEVVRIPGEVAVRCVGLNCPAQALERVKHFAARRAMDIDGLGEKFIEQLLNLKLIRNVADIYRLT
EEDFMQFERMGKKLAENLLNSIAASKERELSRLIFALGIRHVGEHTAKLLASAFGSMENLAAASEEELTSIREVGPQVAA
SIADFFKSEENLEVLRELKEHGVNPRVEEKRVGGRFTGKTFVFTGALEKFTRDEAKKMVELEGGHAAGSVSKKTDYVVAG
ADAGSKLDKAQQLGVRVLSEDDFLELMQ

Sequences:

>Translated_668_residues
MDKTAAAQRIKWLSSEIERHNRLYYEQDMPEITDAEYDALFRELKELEASYPDLALPDSPTGRVGGRPVAKFTQVRHTTP
MLSLENAFTEKDIVDFDDRMKRFLGLSGAEEIGYVCEPKMDGVAVELVYRDGLLAIGSTRGDGLVGEEVTQNLKTIKDIP
LRLQTEEPPGLLTVRGEVYLPLEPFRKFNQEREEAGEPPFANPRNAAAGSLRQLDSKITAKRPLSIFCYAPGEVDGVAFQ
SQSDFLSTIPTWRIPVNPLTRLVPGVQGVLDYYNEMMEKRDDLPYEIDGVVVKVDRFSLQRDLGEKSRSPRWAIAWKFPP
RQATTVVNDIVPQVGRTGVITPVAHLEPVNVSGVMVSRATLHNWEEMERKDIRRGDTVVVERAGDVIPAVVQVLVEKRQG
SETLLPVPQACPVCGGEVVRIPGEVAVRCVGLNCPAQALERVKHFAARRAMDIDGLGEKFIEQLLNLKLIRNVADIYRLT
EEDFMQFERMGKKLAENLLNSIAASKERELSRLIFALGIRHVGEHTAKLLASAFGSMENLAAASEEELTSIREVGPQVAA
SIADFFKSEENLEVLRELKEHGVNPRVEEKRVGGRFTGKTFVFTGALEKFTRDEAKKMVELEGGHAAGSVSKKTDYVVAG
ADAGSKLDKAQQLGVRVLSEDDFLELMQ
>Mature_668_residues
MDKTAAAQRIKWLSSEIERHNRLYYEQDMPEITDAEYDALFRELKELEASYPDLALPDSPTGRVGGRPVAKFTQVRHTTP
MLSLENAFTEKDIVDFDDRMKRFLGLSGAEEIGYVCEPKMDGVAVELVYRDGLLAIGSTRGDGLVGEEVTQNLKTIKDIP
LRLQTEEPPGLLTVRGEVYLPLEPFRKFNQEREEAGEPPFANPRNAAAGSLRQLDSKITAKRPLSIFCYAPGEVDGVAFQ
SQSDFLSTIPTWRIPVNPLTRLVPGVQGVLDYYNEMMEKRDDLPYEIDGVVVKVDRFSLQRDLGEKSRSPRWAIAWKFPP
RQATTVVNDIVPQVGRTGVITPVAHLEPVNVSGVMVSRATLHNWEEMERKDIRRGDTVVVERAGDVIPAVVQVLVEKRQG
SETLLPVPQACPVCGGEVVRIPGEVAVRCVGLNCPAQALERVKHFAARRAMDIDGLGEKFIEQLLNLKLIRNVADIYRLT
EEDFMQFERMGKKLAENLLNSIAASKERELSRLIFALGIRHVGEHTAKLLASAFGSMENLAAASEEELTSIREVGPQVAA
SIADFFKSEENLEVLRELKEHGVNPRVEEKRVGGRFTGKTFVFTGALEKFTRDEAKKMVELEGGHAAGSVSKKTDYVVAG
ADAGSKLDKAQQLGVRVLSEDDFLELMQ

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=72, Percent_Identity=51.3888888888889, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1788750, Length=667, Percent_Identity=47.8260869565217, Blast_Score=610, Evalue=1e-175,
Organism=Escherichia coli, GI87082305, Length=518, Percent_Identity=27.2200772200772, Blast_Score=122, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_GEOBB (B5EIJ0)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002138151.1
- ProteinModelPortal:   B5EIJ0
- GeneID:   6781323
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_1336
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK828084
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74328; Mature: 74328

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 119-119 BINDING 117-117 BINDING 140-140 BINDING 177-177 BINDING 293-293 BINDING 317-317

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKTAAAQRIKWLSSEIERHNRLYYEQDMPEITDAEYDALFRELKELEASYPDLALPDSP
CCCHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
TGRVGGRPVAKFTQVRHTTPMLSLENAFTEKDIVDFDDRMKRFLGLSGAEEIGYVCEPKM
CCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHCCCCCCCCC
DGVAVELVYRDGLLAIGSTRGDGLVGEEVTQNLKTIKDIPLRLQTEEPPGLLTVRGEVYL
CCEEEEEEEECCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCEEEECCCEEE
PLEPFRKFNQEREEAGEPPFANPRNAAAGSLRQLDSKITAKRPLSIFCYAPGEVDGVAFQ
CHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEC
SQSDFLSTIPTWRIPVNPLTRLVPGVQGVLDYYNEMMEKRDDLPYEIDGVVVKVDRFSLQ
CCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEEHHHHHH
RDLGEKSRSPRWAIAWKFPPRQATTVVNDIVPQVGRTGVITPVAHLEPVNVSGVMVSRAT
HHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCEEEHHHH
LHNWEEMERKDIRRGDTVVVERAGDVIPAVVQVLVEKRQGSETLLPVPQACPVCGGEVVR
HHCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEE
IPGEVAVRCVGLNCPAQALERVKHFAARRAMDIDGLGEKFIEQLLNLKLIRNVADIYRLT
CCHHHEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
EEDFMQFERMGKKLAENLLNSIAASKERELSRLIFALGIRHVGEHTAKLLASAFGSMENL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
AAASEEELTSIREVGPQVAASIADFFKSEENLEVLRELKEHGVNPRVEEKRVGGRFTGKT
HHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHCCCEECCCE
FVFTGALEKFTRDEAKKMVELEGGHAAGSVSKKTDYVVAGADAGSKLDKAQQLGVRVLSE
EEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHCCEEECC
DDFLELMQ
CHHHHHCC
>Mature Secondary Structure
MDKTAAAQRIKWLSSEIERHNRLYYEQDMPEITDAEYDALFRELKELEASYPDLALPDSP
CCCHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
TGRVGGRPVAKFTQVRHTTPMLSLENAFTEKDIVDFDDRMKRFLGLSGAEEIGYVCEPKM
CCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHCCCCCCCCC
DGVAVELVYRDGLLAIGSTRGDGLVGEEVTQNLKTIKDIPLRLQTEEPPGLLTVRGEVYL
CCEEEEEEEECCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCEEEECCCEEE
PLEPFRKFNQEREEAGEPPFANPRNAAAGSLRQLDSKITAKRPLSIFCYAPGEVDGVAFQ
CHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEC
SQSDFLSTIPTWRIPVNPLTRLVPGVQGVLDYYNEMMEKRDDLPYEIDGVVVKVDRFSLQ
CCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEEHHHHHH
RDLGEKSRSPRWAIAWKFPPRQATTVVNDIVPQVGRTGVITPVAHLEPVNVSGVMVSRAT
HHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCEEEHHHH
LHNWEEMERKDIRRGDTVVVERAGDVIPAVVQVLVEKRQGSETLLPVPQACPVCGGEVVR
HHCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEE
IPGEVAVRCVGLNCPAQALERVKHFAARRAMDIDGLGEKFIEQLLNLKLIRNVADIYRLT
CCHHHEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
EEDFMQFERMGKKLAENLLNSIAASKERELSRLIFALGIRHVGEHTAKLLASAFGSMENL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
AAASEEELTSIREVGPQVAASIADFFKSEENLEVLRELKEHGVNPRVEEKRVGGRFTGKT
HHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHCCCEECCCE
FVFTGALEKFTRDEAKKMVELEGGHAAGSVSKKTDYVVAGADAGSKLDKAQQLGVRVLSE
EEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHCCEEECC
DDFLELMQ
CHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA