Click here to switch to the map view.

The map label for this gene is pulF [H]

Identifier: 197117666

GI number: 197117666

Start: 1475672

End: 1476877

Strand: Direct

Name: pulF [H]

Synonym: Gbem_1278

Alternate gene names: 197117666

Gene position: 1475672-1476877 (Clockwise)

Preceding gene: 197117665

Following gene: 197117667

Centisome position: 31.97

GC content: 61.53

Gene sequence:

>1206_bases
ATGCCCGTCTTTACCTGTAAAATAGGCGCCTCCGACGGCAAGATCCTGGTCAAGGACCTCGACGCGGTCAACGCCGGCTT
GCTCCGGCAGAGCCTGGAAGAGCAAGGGTACGTCGTCTTCGAGGTGCGCAAGAAGCCGTTCCAGTTCCTGCTGGAATCGG
GGATCGGGCGCAAGAAGATCGGCAACAAGGAGCTGCTGTTGTTCAACCAGGAACTCCTGGTACTGCTCAAGGCCGGACTC
CCCATCCTGCAGGCGCTCGACACCATCCTGGAGTCGGGAGGGGGCAAGCTCAACGAGATACTGTCGGCGATACGCGAGGA
CGTGAAAGGTGGAATGGCGCTCTCCGCCGCCTTCGAGAAGTTTCCGAGGGTGTTTCCCCATCTCTACATCGCGTCGGTCC
GGGCCGGGGAGAGGACCGGGGACCTGCCGCAGACCATCCGCCGTTACATCGCCTTTCTCAAGAGAACCGAGGGGTTCCGC
GGCAAGGTCATCGGCGCGCTTATCTACCCTGCCATCCTGGTCACGGTCGCGGCGGTGGCGGTCTCTTTGTTGCTCATCTA
CGTGGTGCCGACCTTCAGTACCATCTACGCGGATTCCGGCGCCGCTTTGCCGCTCCCGACCCAGATGCTGATCAATTTCA
CCGGGCTCTTGCGGCACTACCTGCCGCTACTTCTGCTGCTGGGGGGCGTGGCGATCACCCTCTTCAAGCGCTGGAGCCAG
ACCGAATCCGGACGCTATACGGTGGACGGCTTCAAGATCAAGACCCCGCTTCTGGGTGCCGTCACCAGCCGTTACGCCCT
TGCCGGCTTTACCCGTACCCTGGCCACCGTGCTGGGCTCCGGTATCCCCATCGTCGAGGCGATGCGGATGTCGGTGGGGA
CGCTCAACAACAAGGTCCTGGAGCGCGGCCTGCTCCTTGCAGTGCATCGCGTAGAGGAGGGGAGCAAGCTCTCCACCGCG
CTGGAAGGTATGAAGCTGATGCCCCCACTGGCGCTGCGCATGCTCACCGTCGGCGAGACCACCGGCTCATTGGAGGAGAT
GCTATCGGACATCTCCGATTACTTCGAAGAGGAGATCGAAAGGGATCTCCATGTACTGACCACCTCGATCGAACCGGCGA
TCATGGTGGCCATGGGTGTGGTCATCGGGGTCATCATCGTCACCATGTACCTGCCGATCTTCAAGATCGCCAGCACTGTC
GGCTAG

Upstream 100 bases:

>100_bases
GTGGATCGTCGGCATGACTCAGGCCTGACCGCCGCAGTAGACAGAATTGGTTTTCTTTTAAGTTTTAATTCAGCCCTATT
TGTGGTACTCATAGTGCCTT

Downstream 100 bases:

>100_bases
AGGGATCATGCTGAAACCTTTCCGAAAGAAAAGACTGGGCGACATCCTGCTGGAGCGGGGGGACCTGACTCAGGACCAGC
TCGCCTACGCGCTGGACAAG

Product: type II secretion system protein PulF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MPVFTCKIGASDGKILVKDLDAVNAGLLRQSLEEQGYVVFEVRKKPFQFLLESGIGRKKIGNKELLLFNQELLVLLKAGL
PILQALDTILESGGGKLNEILSAIREDVKGGMALSAAFEKFPRVFPHLYIASVRAGERTGDLPQTIRRYIAFLKRTEGFR
GKVIGALIYPAILVTVAAVAVSLLLIYVVPTFSTIYADSGAALPLPTQMLINFTGLLRHYLPLLLLLGGVAITLFKRWSQ
TESGRYTVDGFKIKTPLLGAVTSRYALAGFTRTLATVLGSGIPIVEAMRMSVGTLNNKVLERGLLLAVHRVEEGSKLSTA
LEGMKLMPPLALRMLTVGETTGSLEEMLSDISDYFEEEIERDLHVLTTSIEPAIMVAMGVVIGVIIVTMYLPIFKIASTV
G

Sequences:

>Translated_401_residues
MPVFTCKIGASDGKILVKDLDAVNAGLLRQSLEEQGYVVFEVRKKPFQFLLESGIGRKKIGNKELLLFNQELLVLLKAGL
PILQALDTILESGGGKLNEILSAIREDVKGGMALSAAFEKFPRVFPHLYIASVRAGERTGDLPQTIRRYIAFLKRTEGFR
GKVIGALIYPAILVTVAAVAVSLLLIYVVPTFSTIYADSGAALPLPTQMLINFTGLLRHYLPLLLLLGGVAITLFKRWSQ
TESGRYTVDGFKIKTPLLGAVTSRYALAGFTRTLATVLGSGIPIVEAMRMSVGTLNNKVLERGLLLAVHRVEEGSKLSTA
LEGMKLMPPLALRMLTVGETTGSLEEMLSDISDYFEEEIERDLHVLTTSIEPAIMVAMGVVIGVIIVTMYLPIFKIASTV
G
>Mature_400_residues
PVFTCKIGASDGKILVKDLDAVNAGLLRQSLEEQGYVVFEVRKKPFQFLLESGIGRKKIGNKELLLFNQELLVLLKAGLP
ILQALDTILESGGGKLNEILSAIREDVKGGMALSAAFEKFPRVFPHLYIASVRAGERTGDLPQTIRRYIAFLKRTEGFRG
KVIGALIYPAILVTVAAVAVSLLLIYVVPTFSTIYADSGAALPLPTQMLINFTGLLRHYLPLLLLLGGVAITLFKRWSQT
ESGRYTVDGFKIKTPLLGAVTSRYALAGFTRTLATVLGSGIPIVEAMRMSVGTLNNKVLERGLLLAVHRVEEGSKLSTAL
EGMKLMPPLALRMLTVGETTGSLEEMLSDISDYFEEEIERDLHVLTTSIEPAIMVAMGVVIGVIIVTMYLPIFKIASTVG

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=395, Percent_Identity=29.1139240506329, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1786295, Length=335, Percent_Identity=28.3582089552239, Blast_Score=115, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 43609; Mature: 43478

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVFTCKIGASDGKILVKDLDAVNAGLLRQSLEEQGYVVFEVRKKPFQFLLESGIGRKKI
CCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHCCCCCCCC
GNKELLLFNQELLVLLKAGLPILQALDTILESGGGKLNEILSAIREDVKGGMALSAAFEK
CCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
FPRVFPHLYIASVRAGERTGDLPQTIRRYIAFLKRTEGFRGKVIGALIYPAILVTVAAVA
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
VSLLLIYVVPTFSTIYADSGAALPLPTQMLINFTGLLRHYLPLLLLLGGVAITLFKRWSQ
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TESGRYTVDGFKIKTPLLGAVTSRYALAGFTRTLATVLGSGIPIVEAMRMSVGTLNNKVL
CCCCCEEECCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
ERGLLLAVHRVEEGSKLSTALEGMKLMPPLALRMLTVGETTGSLEEMLSDISDYFEEEIE
HHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
RDLHVLTTSIEPAIMVAMGVVIGVIIVTMYLPIFKIASTVG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PVFTCKIGASDGKILVKDLDAVNAGLLRQSLEEQGYVVFEVRKKPFQFLLESGIGRKKI
CEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHCCCCCCCC
GNKELLLFNQELLVLLKAGLPILQALDTILESGGGKLNEILSAIREDVKGGMALSAAFEK
CCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
FPRVFPHLYIASVRAGERTGDLPQTIRRYIAFLKRTEGFRGKVIGALIYPAILVTVAAVA
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
VSLLLIYVVPTFSTIYADSGAALPLPTQMLINFTGLLRHYLPLLLLLGGVAITLFKRWSQ
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TESGRYTVDGFKIKTPLLGAVTSRYALAGFTRTLATVLGSGIPIVEAMRMSVGTLNNKVL
CCCCCEEECCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
ERGLLLAVHRVEEGSKLSTALEGMKLMPPLALRMLTVGETTGSLEEMLSDISDYFEEEIE
HHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
RDLHVLTTSIEPAIMVAMGVVIGVIIVTMYLPIFKIASTVG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]