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The map label for this gene is pulE-1 [H]

Identifier: 197117667

GI number: 197117667

Start: 1476885

End: 1478606

Strand: Direct

Name: pulE-1 [H]

Synonym: Gbem_1279

Alternate gene names: 197117667

Gene position: 1476885-1478606 (Clockwise)

Preceding gene: 197117666

Following gene: 197117668

Centisome position: 32.0

GC content: 63.01

Gene sequence:

>1722_bases
ATGCTGAAACCTTTCCGAAAGAAAAGACTGGGCGACATCCTGCTGGAGCGGGGGGACCTGACTCAGGACCAGCTCGCCTA
CGCGCTGGACAAGCAGCATAGCACCAAGGAACGACTGGGGATCATCTGCCTGGCCGACGGGCTGATCAGCGACATCGTGC
TGGCCATGGCGCTCGCCGAGCAGTTCGGCCTCGAGTACGTGGACCTGAAGGGGGTGCGCCCGGACGAGTCCCTGTTCAAC
TTCATCCCGCCCGAGGTCACGTTCCGCTTCCACTTCGTGCCGCTGGATTTGCAGGGGGACCTGCTGGTGGTGGCCATCTC
AGACCCCACCGCAGTGCTGCAGCTGGATGAGATGGCGCTCCTTCTCGAGCGCCCGCTGCTTTTCAAGGTGGCCGCCGAAT
CGGAAATCGCCTACCATCTGAAACGGGGCGAGGGGACCAGCCGGGTGCTGAAGGAGGTCTCCGAGGACTTCATGCTGCAG
CTGGTGACCGAGACCGACAAAGGGGAGGAGATTCTCTCCGTCGAGAACGTCACCGCGGACACGAGCCCCATCATCAAGCT
GGTGAACTCCACCATACTGGATGCCCTCACCAGGCGCGCCTCCGATATTCATATCGAGACCGCGCAGGACGGCGTCCTCA
TCAAGTACCGCATCGACGGCGTCCTCTACAAGGCCACCGACCCCATCGACTCCCATTTCCAGGGGCCGCTCATCTCCCGT
CTCAAGGTCATGAGCGAACTGGACATCTCGGAGCGCCGCATCCCGCAGGACGGCCGCTTCAAGGTCCGCATCGGCTCCAA
GTCCATCGACTTCCGCGTCTCCATCATGCCTAGCGCCTTCGGCGAGGACGCGGTCATCCGTATCCTCGACAAGGAGAGCA
TCGCCAGCGACCTGCGGGGGCTTACCCTGGAGACGCTGGGGATGGCGCCCCGCGAGATCAAGCGCTTCCGGAAGATGATC
AAGGAGCCCTACGGCATGGTGCTGGTAACCGGGCCGACCGGTTCGGGCAAGACCACCACCCTCTACGGAGCCTTGACCGA
GATACATACCGGTCTGGAAAAGATCATCACCATCGAGGACCCGGTCGAGTACGTCCTCTCCGGCATCACCCAGATCCCAG
TCAACGAGAAGAAGGGGCTCACCTTCGCCCGGGGCCTGCGCTCCGTCCTGCGCCACGACCCGGACAAGATCATGGTGGGT
GAGATCCGCGACTCGGAGACGGCGCAGATCGCGGTGCAGTCGGCGCTCACAGGCCACCTGGTGTTCACCACGGTGCACGC
CAACAACGTCTTCGACGTCATCGGCCGCTTCACCCACATGGGAATCGACCCCTACAACTTCGTGGCCTGCCTTAACTGCG
TCATGGCGCAACGCCTGGTCAGGAAGATCTGCCCCGCCTGCCGCAAGCCGGTGACGGTTACCGACGACGAACTGCGCGAG
TCGGCGATCGACCCGGAAAGCACCCGCGGCCTGACCCTCTACGAGGCCCGTGGATGCGACGAGTGCAACGGTACCGGCTA
CCGCGGCCGCTCCGCCATCGTCGAGCTTTTGGACCTGAACGACGAGATCAGGGAACTGATCATCGCCAAGTCACCGGTGG
CCGCCCTCAAAAAGGCCGCCAAGGCTGCAGGAACCATGTTCCTCCGGGAGAGCGCGGTAGCCAAGGTGCTGGCGGGCGAG
ACCACGATAAAGGAGATCAACCGTGTCACTTTCGTGGAGTAG

Upstream 100 bases:

>100_bases
CCGGCGATCATGGTGGCCATGGGTGTGGTCATCGGGGTCATCATCGTCACCATGTACCTGCCGATCTTCAAGATCGCCAG
CACTGTCGGCTAGAGGGATC

Downstream 100 bases:

>100_bases
GTGCCAGCCATGCTAACGAACAGGAAGGGCCTGGGACTCGAGATCTCCGCCGGCGGGCTCAGCTTCGCCATGGTGAGCGG
CGGCAAAACACCCAAGGTGG

Product: type II secretion system ATPase PulE

Products: NA

Alternate protein names: Pectic enzymes secretion protein outE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MLKPFRKKRLGDILLERGDLTQDQLAYALDKQHSTKERLGIICLADGLISDIVLAMALAEQFGLEYVDLKGVRPDESLFN
FIPPEVTFRFHFVPLDLQGDLLVVAISDPTAVLQLDEMALLLERPLLFKVAAESEIAYHLKRGEGTSRVLKEVSEDFMLQ
LVTETDKGEEILSVENVTADTSPIIKLVNSTILDALTRRASDIHIETAQDGVLIKYRIDGVLYKATDPIDSHFQGPLISR
LKVMSELDISERRIPQDGRFKVRIGSKSIDFRVSIMPSAFGEDAVIRILDKESIASDLRGLTLETLGMAPREIKRFRKMI
KEPYGMVLVTGPTGSGKTTTLYGALTEIHTGLEKIITIEDPVEYVLSGITQIPVNEKKGLTFARGLRSVLRHDPDKIMVG
EIRDSETAQIAVQSALTGHLVFTTVHANNVFDVIGRFTHMGIDPYNFVACLNCVMAQRLVRKICPACRKPVTVTDDELRE
SAIDPESTRGLTLYEARGCDECNGTGYRGRSAIVELLDLNDEIRELIIAKSPVAALKKAAKAAGTMFLRESAVAKVLAGE
TTIKEINRVTFVE

Sequences:

>Translated_573_residues
MLKPFRKKRLGDILLERGDLTQDQLAYALDKQHSTKERLGIICLADGLISDIVLAMALAEQFGLEYVDLKGVRPDESLFN
FIPPEVTFRFHFVPLDLQGDLLVVAISDPTAVLQLDEMALLLERPLLFKVAAESEIAYHLKRGEGTSRVLKEVSEDFMLQ
LVTETDKGEEILSVENVTADTSPIIKLVNSTILDALTRRASDIHIETAQDGVLIKYRIDGVLYKATDPIDSHFQGPLISR
LKVMSELDISERRIPQDGRFKVRIGSKSIDFRVSIMPSAFGEDAVIRILDKESIASDLRGLTLETLGMAPREIKRFRKMI
KEPYGMVLVTGPTGSGKTTTLYGALTEIHTGLEKIITIEDPVEYVLSGITQIPVNEKKGLTFARGLRSVLRHDPDKIMVG
EIRDSETAQIAVQSALTGHLVFTTVHANNVFDVIGRFTHMGIDPYNFVACLNCVMAQRLVRKICPACRKPVTVTDDELRE
SAIDPESTRGLTLYEARGCDECNGTGYRGRSAIVELLDLNDEIRELIIAKSPVAALKKAAKAAGTMFLRESAVAKVLAGE
TTIKEINRVTFVE
>Mature_573_residues
MLKPFRKKRLGDILLERGDLTQDQLAYALDKQHSTKERLGIICLADGLISDIVLAMALAEQFGLEYVDLKGVRPDESLFN
FIPPEVTFRFHFVPLDLQGDLLVVAISDPTAVLQLDEMALLLERPLLFKVAAESEIAYHLKRGEGTSRVLKEVSEDFMLQ
LVTETDKGEEILSVENVTADTSPIIKLVNSTILDALTRRASDIHIETAQDGVLIKYRIDGVLYKATDPIDSHFQGPLISR
LKVMSELDISERRIPQDGRFKVRIGSKSIDFRVSIMPSAFGEDAVIRILDKESIASDLRGLTLETLGMAPREIKRFRKMI
KEPYGMVLVTGPTGSGKTTTLYGALTEIHTGLEKIITIEDPVEYVLSGITQIPVNEKKGLTFARGLRSVLRHDPDKIMVG
EIRDSETAQIAVQSALTGHLVFTTVHANNVFDVIGRFTHMGIDPYNFVACLNCVMAQRLVRKICPACRKPVTVTDDELRE
SAIDPESTRGLTLYEARGCDECNGTGYRGRSAIVELLDLNDEIRELIIAKSPVAALKKAAKAAGTMFLRESAVAKVLAGE
TTIKEINRVTFVE

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pectate lyases and cellulases across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=441, Percent_Identity=41.0430839002268, Blast_Score=337, Evalue=9e-94,
Organism=Escherichia coli, GI1786296, Length=493, Percent_Identity=36.5111561866126, Blast_Score=301, Evalue=8e-83,
Organism=Escherichia coli, GI87082188, Length=280, Percent_Identity=33.2142857142857, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 63492; Mature: 63492

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKPFRKKRLGDILLERGDLTQDQLAYALDKQHSTKERLGIICLADGLISDIVLAMALAE
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHEEEEECHHHHHHHHHHHHHHH
QFGLEYVDLKGVRPDESLFNFIPPEVTFRFHFVPLDLQGDLLVVAISDPTAVLQLDEMAL
HHCCEEEEECCCCCCHHHHHCCCCCEEEEEEEEEEECCCCEEEEEECCCCEEEEHHHHHH
LLERPLLFKVAAESEIAYHLKRGEGTSRVLKEVSEDFMLQLVTETDKGEEILSVENVTAD
HHCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHEEHHHCCCCCCEEEEECCCCCC
TSPIIKLVNSTILDALTRRASDIHIETAQDGVLIKYRIDGVLYKATDPIDSHFQGPLISR
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECEEEEEECCCCCCCCCCHHHHH
LKVMSELDISERRIPQDGRFKVRIGSKSIDFRVSIMPSAFGEDAVIRILDKESIASDLRG
HHHHHHCCCHHHCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCEEEEECHHHHHHHHCC
LTLETLGMAPREIKRFRKMIKEPYGMVLVTGPTGSGKTTTLYGALTEIHTGLEKIITIED
CCHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEHHHHHHHHHCCHHHEEECCC
PVEYVLSGITQIPVNEKKGLTFARGLRSVLRHDPDKIMVGEIRDSETAQIAVQSALTGHL
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCEE
VFTTVHANNVFDVIGRFTHMGIDPYNFVACLNCVMAQRLVRKICPACRKPVTVTDDELRE
EEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
SAIDPESTRGLTLYEARGCDECNGTGYRGRSAIVELLDLNDEIRELIIAKSPVAALKKAA
HCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHH
KAAGTMFLRESAVAKVLAGETTIKEINRVTFVE
HHHHHHHHHHHHHHHHHCCCHHHHHHCHHCCCC
>Mature Secondary Structure
MLKPFRKKRLGDILLERGDLTQDQLAYALDKQHSTKERLGIICLADGLISDIVLAMALAE
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHEEEEECHHHHHHHHHHHHHHH
QFGLEYVDLKGVRPDESLFNFIPPEVTFRFHFVPLDLQGDLLVVAISDPTAVLQLDEMAL
HHCCEEEEECCCCCCHHHHHCCCCCEEEEEEEEEEECCCCEEEEEECCCCEEEEHHHHHH
LLERPLLFKVAAESEIAYHLKRGEGTSRVLKEVSEDFMLQLVTETDKGEEILSVENVTAD
HHCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHEEHHHCCCCCCEEEEECCCCCC
TSPIIKLVNSTILDALTRRASDIHIETAQDGVLIKYRIDGVLYKATDPIDSHFQGPLISR
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECEEEEEECCCCCCCCCCHHHHH
LKVMSELDISERRIPQDGRFKVRIGSKSIDFRVSIMPSAFGEDAVIRILDKESIASDLRG
HHHHHHCCCHHHCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCEEEEECHHHHHHHHCC
LTLETLGMAPREIKRFRKMIKEPYGMVLVTGPTGSGKTTTLYGALTEIHTGLEKIITIED
CCHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEHHHHHHHHHCCHHHEEECCC
PVEYVLSGITQIPVNEKKGLTFARGLRSVLRHDPDKIMVGEIRDSETAQIAVQSALTGHL
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCEE
VFTTVHANNVFDVIGRFTHMGIDPYNFVACLNCVMAQRLVRKICPACRKPVTVTDDELRE
EEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
SAIDPESTRGLTLYEARGCDECNGTGYRGRSAIVELLDLNDEIRELIIAKSPVAALKKAA
HCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHH
KAAGTMFLRESAVAKVLAGETTIKEINRVTFVE
HHHHHHHHHHHHHHHHHCCCHHHHHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]