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The map label for this gene is hup-2 [H]

Identifier: 197117663

GI number: 197117663

Start: 1473762

End: 1474037

Strand: Direct

Name: hup-2 [H]

Synonym: Gbem_1275

Alternate gene names: 197117663

Gene position: 1473762-1474037 (Clockwise)

Preceding gene: 197117662

Following gene: 197117664

Centisome position: 31.93

GC content: 56.88

Gene sequence:

>276_bases
ATGAATAAGGCAGAGCTGGTAAGCGCCATAGCAGAAGAAGCCCAGTTAACCAAGGTTGATGCTGAGAAGTCCCTCATGGG
AATTTTGGACGCACTGACCAACTGCCTCGCCACCGGCGACAAGGTGACCTTGGTAGGTTTCGGTACCTTCAGCGTCGCCG
AGCGCGCCGCCAGGACCGGCCAGAATCCGCAGACCGGCAAGAAGATCGAGATTGCCGCTTCGTCTGCGCCGAAGTTCAAG
CCGGGCAACACCTTGAAGGACCTGGTAAACAGCTAA

Upstream 100 bases:

>100_bases
TGTGCCACATTAGAAAAAAAGCCGCAGAAAAAAGGTTGACAGTCGAGACGGCGCTCTGGTATAAGATGTCTTCACTTTGC
GATGCAAACCGGGAGGATCA

Downstream 100 bases:

>100_bases
CCCTGTTTTTGCCGGAAATGCTGTACATGCGGGGTTGTAGCTCAGTTGGTTAGAGTGCCAGCCTGTCACGCTGGAAGTCG
CGGGTTCGAGCCCCGTCAAC

Product: histone-like protein

Products: NA

Alternate protein names: DNA-binding protein II; HB [H]

Number of amino acids: Translated: 91; Mature: 91

Protein sequence:

>91_residues
MNKAELVSAIAEEAQLTKVDAEKSLMGILDALTNCLATGDKVTLVGFGTFSVAERAARTGQNPQTGKKIEIAASSAPKFK
PGNTLKDLVNS

Sequences:

>Translated_91_residues
MNKAELVSAIAEEAQLTKVDAEKSLMGILDALTNCLATGDKVTLVGFGTFSVAERAARTGQNPQTGKKIEIAASSAPKFK
PGNTLKDLVNS
>Mature_91_residues
MNKAELVSAIAEEAQLTKVDAEKSLMGILDALTNCLATGDKVTLVGFGTFSVAERAARTGQNPQTGKKIEIAASSAPKFK
PGNTLKDLVNS

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=90, Percent_Identity=54.4444444444444, Blast_Score=100, Evalue=3e-23,
Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=55.0561797752809, Blast_Score=98, Evalue=1e-22,
Organism=Escherichia coli, GI1787141, Length=91, Percent_Identity=37.3626373626374, Blast_Score=79, Evalue=5e-17,
Organism=Escherichia coli, GI1788005, Length=91, Percent_Identity=32.967032967033, Blast_Score=62, Evalue=8e-12,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 9493; Mature: 9493

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKAELVSAIAEEAQLTKVDAEKSLMGILDALTNCLATGDKVTLVGFGTFSVAERAARTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCC
QNPQTGKKIEIAASSAPKFKPGNTLKDLVNS
CCCCCCCEEEEEECCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MNKAELVSAIAEEAQLTKVDAEKSLMGILDALTNCLATGDKVTLVGFGTFSVAERAARTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCC
QNPQTGKKIEIAASSAPKFKPGNTLKDLVNS
CCCCCCCEEEEEECCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1644313 [H]