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The map label for this gene is pyk [H]

Identifier: 197117570

GI number: 197117570

Start: 1377208

End: 1378653

Strand: Direct

Name: pyk [H]

Synonym: Gbem_1182

Alternate gene names: 197117570

Gene position: 1377208-1378653 (Clockwise)

Preceding gene: 197117569

Following gene: 197117571

Centisome position: 29.84

GC content: 64.18

Gene sequence:

>1446_bases
ATGTTCCGTCACACAAAGATCGTTGCCACTGTCGGACCCGCCAGCGAATCCGAAGAGATGCTCGGCGCCTTGATAAAGGC
CGGCGTCGACGTTTTCCGCCTCAACTTCTCCCATGGCGACCATGCCGCCAAATCCGCCGTCATCCGCAGCATCCGCAAAC
TCTGCGACCAACATGAGCATTCTGTCGCCATCCTCGGGGACCTGCAGGGGCCGAAGATCCGCACCGGCCTCATGAAGGGG
GGCGGGATGCAGCTCACCGCCGGGACCGAAGTGATCGTCACCACCCGTGAGCTGCTAGGCGAGGGGAGCCTCATCCCGAC
CATCTACCAGGGGCTCCCCGGGGACGTGAAGCCCGACGACCGCATCCTTTTGGACGACGGCCTGATGGAGCTGAGGGTGC
TGGGGGTGGAAGGGGGCGATGTGCGCTGCCTCGTCGTGACCGGAGGCCTCCTCAAGGACCGCAAGGGGATCAACCTCCCC
GGCGCCAAGGTTTCCGCCCCCGCCATGACCGGCAAGGACCGCGACGACCTCGAATTCTGCATGGAGCAGCGGCTTGACTA
CGTCGCCCTTTCCTTCGTGCGCGAGGCATCCGACGTGCAGGAATTGAGGAGCATCATCGACAGCCGCGGCTCCCAGCTCC
GCATCATCTCCAAGATCGAGAAGCCGGAGGCGGTGCTCAACTTCGGCGCCATCTTGCAGGCTTCCGACGGGGTCATGGTG
GCCCGAGGCGACCTGGGGGTGGAGTTGAATCCCGAGAAGGTTCCGCTCATCCAGAAGCGGATCATCCGCAGTTGCAACGA
TGCCGGAAAGCCGGTGATCACCGCCACCCAAATGCTGGAGAGCATGGTGAACAACCCGCGCCCGACCCGCGCCGAGACCT
CCGACGTCGCCAACGCCATCCTGGACGGCACCGACGCCATCATGCTTTCCGCCGAGACCGCGTCCGGCAAGTATCCGGTG
GAGGCGGTCTCCATGATGGTGCAGGTGGCGCGCGACGTGGAAGCGGACCCGCAGCTCATGGCGCAAAGCTGCCACATCGC
CCAGACCGAGGCGGAGCCGAACCTCTCCGAGGTGATCGGGCTTGCCGCCTGCCATGCGGCGGAAAGCGTCAAGGCATCGG
CGATCCTTGCCTTCACCCAGACCGGAGGGACCGCGGCTCTCGTTTCCAAGTGCCGGCCGGCCCAGCCCATCATCGCCGTG
ACACCATCCGAAGAGGTGCGGCGCGTGCTCGCCCTTTATGCCGGGGTGCATTCGCTGCGCGTAGATATCGAGGGGGACAC
TGAATCCCAGATCATCTCCGTCGAGAAAGCGGTGCTGGAAACCGGCTGGCTCAACAAGGGGGAGTTGGTGGTGATAACCA
TGGGAAGCCCGCTTTCTTCAGCGGGAACCACCAACCTGATGAAGGTGCATCGGCTGGGGGAGCCTGAGGTTAAGCGGCGT
CTTTGA

Upstream 100 bases:

>100_bases
CGCGCGGATTTCGGGTAATTTGTACAGGCGCCGGCGCGCGCAGGGGCTTCAAGCCCGGGTGCCGTTGCCGGCCGCTCATT
CCCGCCTACGAGGAGATCCC

Downstream 100 bases:

>100_bases
TTCGGCGCCGCGATAGCTGTGAGCGATGAGCATGAAGAAGAGGGGACGGCGCGCTGCTGTCCCTTCTTTTTTTTACCCTG
TGTGCTGGAATCGCTTGACC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MFRHTKIVATVGPASESEEMLGALIKAGVDVFRLNFSHGDHAAKSAVIRSIRKLCDQHEHSVAILGDLQGPKIRTGLMKG
GGMQLTAGTEVIVTTRELLGEGSLIPTIYQGLPGDVKPDDRILLDDGLMELRVLGVEGGDVRCLVVTGGLLKDRKGINLP
GAKVSAPAMTGKDRDDLEFCMEQRLDYVALSFVREASDVQELRSIIDSRGSQLRIISKIEKPEAVLNFGAILQASDGVMV
ARGDLGVELNPEKVPLIQKRIIRSCNDAGKPVITATQMLESMVNNPRPTRAETSDVANAILDGTDAIMLSAETASGKYPV
EAVSMMVQVARDVEADPQLMAQSCHIAQTEAEPNLSEVIGLAACHAAESVKASAILAFTQTGGTAALVSKCRPAQPIIAV
TPSEEVRRVLALYAGVHSLRVDIEGDTESQIISVEKAVLETGWLNKGELVVITMGSPLSSAGTTNLMKVHRLGEPEVKRR
L

Sequences:

>Translated_481_residues
MFRHTKIVATVGPASESEEMLGALIKAGVDVFRLNFSHGDHAAKSAVIRSIRKLCDQHEHSVAILGDLQGPKIRTGLMKG
GGMQLTAGTEVIVTTRELLGEGSLIPTIYQGLPGDVKPDDRILLDDGLMELRVLGVEGGDVRCLVVTGGLLKDRKGINLP
GAKVSAPAMTGKDRDDLEFCMEQRLDYVALSFVREASDVQELRSIIDSRGSQLRIISKIEKPEAVLNFGAILQASDGVMV
ARGDLGVELNPEKVPLIQKRIIRSCNDAGKPVITATQMLESMVNNPRPTRAETSDVANAILDGTDAIMLSAETASGKYPV
EAVSMMVQVARDVEADPQLMAQSCHIAQTEAEPNLSEVIGLAACHAAESVKASAILAFTQTGGTAALVSKCRPAQPIIAV
TPSEEVRRVLALYAGVHSLRVDIEGDTESQIISVEKAVLETGWLNKGELVVITMGSPLSSAGTTNLMKVHRLGEPEVKRR
L
>Mature_481_residues
MFRHTKIVATVGPASESEEMLGALIKAGVDVFRLNFSHGDHAAKSAVIRSIRKLCDQHEHSVAILGDLQGPKIRTGLMKG
GGMQLTAGTEVIVTTRELLGEGSLIPTIYQGLPGDVKPDDRILLDDGLMELRVLGVEGGDVRCLVVTGGLLKDRKGINLP
GAKVSAPAMTGKDRDDLEFCMEQRLDYVALSFVREASDVQELRSIIDSRGSQLRIISKIEKPEAVLNFGAILQASDGVMV
ARGDLGVELNPEKVPLIQKRIIRSCNDAGKPVITATQMLESMVNNPRPTRAETSDVANAILDGTDAIMLSAETASGKYPV
EAVSMMVQVARDVEADPQLMAQSCHIAQTEAEPNLSEVIGLAACHAAESVKASAILAFTQTGGTAALVSKCRPAQPIIAV
TPSEEVRRVLALYAGVHSLRVDIEGDTESQIISVEKAVLETGWLNKGELVVITMGSPLSSAGTTNLMKVHRLGEPEVKRR
L

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI32967597, Length=491, Percent_Identity=44.1955193482688, Blast_Score=335, Evalue=4e-92,
Organism=Homo sapiens, GI10835121, Length=491, Percent_Identity=44.1955193482688, Blast_Score=335, Evalue=8e-92,
Organism=Homo sapiens, GI33286418, Length=489, Percent_Identity=42.5357873210634, Blast_Score=333, Evalue=2e-91,
Organism=Homo sapiens, GI33286422, Length=493, Percent_Identity=41.9878296146045, Blast_Score=332, Evalue=5e-91,
Organism=Homo sapiens, GI33286420, Length=493, Percent_Identity=41.9878296146045, Blast_Score=332, Evalue=5e-91,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=44.0972222222222, Blast_Score=212, Evalue=6e-55,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=44.0972222222222, Blast_Score=212, Evalue=6e-55,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=39.8305084745763, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=39.8305084745763, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=38.6046511627907, Blast_Score=135, Evalue=9e-32,
Organism=Escherichia coli, GI1787965, Length=474, Percent_Identity=43.0379746835443, Blast_Score=355, Evalue=4e-99,
Organism=Escherichia coli, GI1788160, Length=477, Percent_Identity=41.5094339622642, Blast_Score=324, Evalue=7e-90,
Organism=Caenorhabditis elegans, GI17544584, Length=480, Percent_Identity=41.875, Blast_Score=344, Evalue=5e-95,
Organism=Caenorhabditis elegans, GI71984413, Length=484, Percent_Identity=40.9090909090909, Blast_Score=301, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI17506829, Length=484, Percent_Identity=40.9090909090909, Blast_Score=301, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI71984406, Length=484, Percent_Identity=40.9090909090909, Blast_Score=301, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI17506831, Length=484, Percent_Identity=40.9090909090909, Blast_Score=301, Evalue=6e-82,
Organism=Saccharomyces cerevisiae, GI6319279, Length=482, Percent_Identity=38.3817427385892, Blast_Score=287, Evalue=3e-78,
Organism=Saccharomyces cerevisiae, GI6324923, Length=494, Percent_Identity=37.246963562753, Blast_Score=283, Evalue=3e-77,
Organism=Drosophila melanogaster, GI24648964, Length=485, Percent_Identity=41.8556701030928, Blast_Score=335, Evalue=6e-92,
Organism=Drosophila melanogaster, GI28571814, Length=485, Percent_Identity=41.8556701030928, Blast_Score=334, Evalue=7e-92,
Organism=Drosophila melanogaster, GI24648966, Length=410, Percent_Identity=42.6829268292683, Blast_Score=299, Evalue=2e-81,
Organism=Drosophila melanogaster, GI24581235, Length=492, Percent_Identity=32.9268292682927, Blast_Score=265, Evalue=4e-71,
Organism=Drosophila melanogaster, GI24646914, Length=279, Percent_Identity=41.2186379928315, Blast_Score=182, Evalue=4e-46,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51386; Mature: 51386

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRHTKIVATVGPASESEEMLGALIKAGVDVFRLNFSHGDHAAKSAVIRSIRKLCDQHEH
CCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCC
SVAILGDLQGPKIRTGLMKGGGMQLTAGTEVIVTTRELLGEGSLIPTIYQGLPGDVKPDD
CEEEEECCCCCEEEEHHCCCCCEEEECCCEEEEEEHHHHCCCCCCHHHHHCCCCCCCCCC
RILLDDGLMELRVLGVEGGDVRCLVVTGGLLKDRKGINLPGAKVSAPAMTGKDRDDLEFC
EEEECCCCEEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
MEQRLDYVALSFVREASDVQELRSIIDSRGSQLRIISKIEKPEAVLNFGAILQASDGVMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHCCCCHHHHHHHHEEECCCCEEE
ARGDLGVELNPEKVPLIQKRIIRSCNDAGKPVITATQMLESMVNNPRPTRAETSDVANAI
EECCCCCEECCCCCCHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCCCCHHHHHHHH
LDGTDAIMLSAETASGKYPVEAVSMMVQVARDVEADPQLMAQSCHIAQTEAEPNLSEVIG
HCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCEECCCCCCHHHHHH
LAACHAAESVKASAILAFTQTGGTAALVSKCRPAQPIIAVTPSEEVRRVLALYAGVHSLR
HHHHHHHHHCCCEEEEEEECCCCHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHCCEEEE
VDIEGDTESQIISVEKAVLETGWLNKGELVVITMGSPLSSAGTTNLMKVHRLGEPEVKRR
EEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCHHHHHC
L
C
>Mature Secondary Structure
MFRHTKIVATVGPASESEEMLGALIKAGVDVFRLNFSHGDHAAKSAVIRSIRKLCDQHEH
CCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCC
SVAILGDLQGPKIRTGLMKGGGMQLTAGTEVIVTTRELLGEGSLIPTIYQGLPGDVKPDD
CEEEEECCCCCEEEEHHCCCCCEEEECCCEEEEEEHHHHCCCCCCHHHHHCCCCCCCCCC
RILLDDGLMELRVLGVEGGDVRCLVVTGGLLKDRKGINLPGAKVSAPAMTGKDRDDLEFC
EEEECCCCEEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
MEQRLDYVALSFVREASDVQELRSIIDSRGSQLRIISKIEKPEAVLNFGAILQASDGVMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHCCCCHHHHHHHHEEECCCCEEE
ARGDLGVELNPEKVPLIQKRIIRSCNDAGKPVITATQMLESMVNNPRPTRAETSDVANAI
EECCCCCEECCCCCCHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCCCCHHHHHHHH
LDGTDAIMLSAETASGKYPVEAVSMMVQVARDVEADPQLMAQSCHIAQTEAEPNLSEVIG
HCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCEECCCCCCHHHHHH
LAACHAAESVKASAILAFTQTGGTAALVSKCRPAQPIIAVTPSEEVRRVLALYAGVHSLR
HHHHHHHHHCCCEEEEEEECCCCHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHCCEEEE
VDIEGDTESQIISVEKAVLETGWLNKGELVVITMGSPLSSAGTTNLMKVHRLGEPEVKRR
EEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286; 9625784 [H]