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The map label for this gene is fcl-2 [H]

Identifier: 197117524

GI number: 197117524

Start: 1313154

End: 1314128

Strand: Reverse

Name: fcl-2 [H]

Synonym: Gbem_1135

Alternate gene names: 197117524

Gene position: 1314128-1313154 (Counterclockwise)

Preceding gene: 197117525

Following gene: 197117523

Centisome position: 28.47

GC content: 60.31

Gene sequence:

>975_bases
ATGAATACCAACGCCAAGATTTTTGTCGCAGGCTCCAAAGGGCTTGTCGGTTCCGCGCTGACACGCAGCCTCAAATCCGC
CGGTTATCGCAATCTCCTCACCCCGGAGAAAGAGCGGCTCGACCTCACCGACCATATCGGGGTCAAGGCCTTCTTCGAAA
GCGAAAAACCGGAGTATGTCGTACTGGCCGCGGCCAAGGTGGGAGGGATACACGCCAACAACACCTACCCTGCCGACTTC
ATCTACCAAAACCTGGTAATCCAGAACAACGTGATCCACCAGGCTCACTTAAACGGTGTGAAGCGTCTGCTCTTCCTCGG
CTCCTCCTGCATCTACCCAAAGCACGCGCCGCAGCCTATGCGAGAGGAGCACCTTCTGACCGGGCCGCTTGAGCCGACCA
ACGAGCCCTACGCCATCGCCAAGATCGCCGGCCTCAAGATGTGCGAGGCCTACAACCGCCAGTACGGCACCAAGTTCATC
GCCGTGATGCCGACCAACCTGTACGGTCCCGGGGACAACTTCGACCTGACCAACTCCCACGTGCTCCCCGCGCTGATCCG
CAAGTTCCACGAATCAAGGGAGCAGCGGCTGCCGGAGGTAGTGGTCTGGGGCACCGGCACCCCGCGCCGCGAATTCCTGT
ACGTGGACGACATGGCGGACGCCTGCCTCCACCTCATGAATCTGCCGGACAGCACCATAACCGAAGAACTCACCGCCTAC
CCGAAGCCCTGTTTCGTCAACCTCGGCACCGGCGTCGACGTCACCATACGGGAGCTGGCGGAAACTGTGCGCGAGGTAGT
CGGCTTCGAGGGGCGGCTTGCCTTCGACACCAACAAGCCTGACGGCACCCCGAGGAAGCTGCAAGAGGTATCCCGGATGA
AGGCGCTGGGATGGGAAGCGAAGGTGACCCTGAAAGACGGCATCGCCAAGTCTTACCAGTGGTTCCTGGAGAACCAGGGC
AACATCCGGAAATAA

Upstream 100 bases:

>100_bases
ACTCGGATCTGGAGGAAGCGCGTCGCGACGAGCTCTGCAAACATCACGGCTTCAGCACCTACAACTACCACGAGTAGCAG
CGAGGACCGTTAGTTCAAAC

Downstream 100 bases:

>100_bases
ACAATAAAAGGGGACAGGCTACTTTTTCAAATCGAAAACTAGCCTGTCCCCTTTTCCCTAATAAGCGCGTAAGCGCTTGA
AATGACTATCAGCAACAATA

Product: GDP-L-fucose synthase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MNTNAKIFVAGSKGLVGSALTRSLKSAGYRNLLTPEKERLDLTDHIGVKAFFESEKPEYVVLAAAKVGGIHANNTYPADF
IYQNLVIQNNVIHQAHLNGVKRLLFLGSSCIYPKHAPQPMREEHLLTGPLEPTNEPYAIAKIAGLKMCEAYNRQYGTKFI
AVMPTNLYGPGDNFDLTNSHVLPALIRKFHESREQRLPEVVVWGTGTPRREFLYVDDMADACLHLMNLPDSTITEELTAY
PKPCFVNLGTGVDVTIRELAETVREVVGFEGRLAFDTNKPDGTPRKLQEVSRMKALGWEAKVTLKDGIAKSYQWFLENQG
NIRK

Sequences:

>Translated_324_residues
MNTNAKIFVAGSKGLVGSALTRSLKSAGYRNLLTPEKERLDLTDHIGVKAFFESEKPEYVVLAAAKVGGIHANNTYPADF
IYQNLVIQNNVIHQAHLNGVKRLLFLGSSCIYPKHAPQPMREEHLLTGPLEPTNEPYAIAKIAGLKMCEAYNRQYGTKFI
AVMPTNLYGPGDNFDLTNSHVLPALIRKFHESREQRLPEVVVWGTGTPRREFLYVDDMADACLHLMNLPDSTITEELTAY
PKPCFVNLGTGVDVTIRELAETVREVVGFEGRLAFDTNKPDGTPRKLQEVSRMKALGWEAKVTLKDGIAKSYQWFLENQG
NIRK
>Mature_324_residues
MNTNAKIFVAGSKGLVGSALTRSLKSAGYRNLLTPEKERLDLTDHIGVKAFFESEKPEYVVLAAAKVGGIHANNTYPADF
IYQNLVIQNNVIHQAHLNGVKRLLFLGSSCIYPKHAPQPMREEHLLTGPLEPTNEPYAIAKIAGLKMCEAYNRQYGTKFI
AVMPTNLYGPGDNFDLTNSHVLPALIRKFHESREQRLPEVVVWGTGTPRREFLYVDDMADACLHLMNLPDSTITEELTAY
PKPCFVNLGTGVDVTIRELAETVREVVGFEGRLAFDTNKPDGTPRKLQEVSRMKALGWEAKVTLKDGIAKSYQWFLENQG
NIRK

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=323, Percent_Identity=33.4365325077399, Blast_Score=184, Evalue=7e-47,
Organism=Homo sapiens, GI7657641, Length=349, Percent_Identity=23.2091690544413, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI42516563, Length=341, Percent_Identity=24.633431085044, Blast_Score=78, Evalue=9e-15,
Organism=Escherichia coli, GI1788365, Length=321, Percent_Identity=58.8785046728972, Blast_Score=404, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17554428, Length=323, Percent_Identity=30.030959752322, Blast_Score=157, Evalue=6e-39,
Organism=Drosophila melanogaster, GI19922778, Length=334, Percent_Identity=31.7365269461078, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI21356223, Length=331, Percent_Identity=26.2839879154079, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 36203; Mature: 36203

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTNAKIFVAGSKGLVGSALTRSLKSAGYRNLLTPEKERLDLTDHIGVKAFFESEKPEYV
CCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHCCEEEEECCCCCCEE
VLAAAKVGGIHANNTYPADFIYQNLVIQNNVIHQAHLNGVKRLLFLGSSCIYPKHAPQPM
EEEEEEECCEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
REEHLLTGPLEPTNEPYAIAKIAGLKMCEAYNRQYGTKFIAVMPTNLYGPGDNFDLTNSH
CCCCCEECCCCCCCCCEEHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCC
VLPALIRKFHESREQRLPEVVVWGTGTPRREFLYVDDMADACLHLMNLPDSTITEELTAY
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEECHHHHHHHHHHCCCCHHHHHHHHCC
PKPCFVNLGTGVDVTIRELAETVREVVGFEGRLAFDTNKPDGTPRKLQEVSRMKALGWEA
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCE
KVTLKDGIAKSYQWFLENQGNIRK
EEEHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNTNAKIFVAGSKGLVGSALTRSLKSAGYRNLLTPEKERLDLTDHIGVKAFFESEKPEYV
CCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHCCEEEEECCCCCCEE
VLAAAKVGGIHANNTYPADFIYQNLVIQNNVIHQAHLNGVKRLLFLGSSCIYPKHAPQPM
EEEEEEECCEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
REEHLLTGPLEPTNEPYAIAKIAGLKMCEAYNRQYGTKFIAVMPTNLYGPGDNFDLTNSH
CCCCCEECCCCCCCCCEEHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCC
VLPALIRKFHESREQRLPEVVVWGTGTPRREFLYVDDMADACLHLMNLPDSTITEELTAY
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEECHHHHHHHHHHCCCCHHHHHHHHCC
PKPCFVNLGTGVDVTIRELAETVREVVGFEGRLAFDTNKPDGTPRKLQEVSRMKALGWEA
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCE
KVTLKDGIAKSYQWFLENQGNIRK
EEEHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]