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The map label for this gene is gmd [H]

Identifier: 197117525

GI number: 197117525

Start: 1314152

End: 1315291

Strand: Reverse

Name: gmd [H]

Synonym: Gbem_1136

Alternate gene names: 197117525

Gene position: 1315291-1314152 (Counterclockwise)

Preceding gene: 197117526

Following gene: 197117524

Centisome position: 28.5

GC content: 60.7

Gene sequence:

>1140_bases
ATGGCAAAAGTAGCTCTTATCACAGGCGTCACTGGACAAGACGGTGCATACCTCGCCGAATTCCTGCTCAAAAAGGGATA
CGTCGTGCACGGCATCAAGCGCCGCGCCTCGCTCTTCAATACGGACCGGATCGACCACCTGTACCAGGATCCGCACATCG
AGAACCGCGACTTCATCCTCCACTACGGCGACCTGACCGACTCCACCAACCTGATCAGGATCATTCAGCAGGTGCAGCCG
GACGAGATCTACAACCTGGCCGCCATGTCGCACGTCGCCGTTTCCTTCGAGACCCCCGAGTACACGGCAAACGCCGACGG
GCTCGGGACGCTGCGGATACTGGAAGCGATCCGCATCCTCGGGCTGGAGAAAAAGACCAGGTTCTACCAGGCCTCCACCA
GCGAGCTTTACGGGCTGGTGCAGGAAGTGCCTCAATCGGAAAAAACCCCCTTCTACCCCCGTTCCCCCTACGCGGTTGCG
AAGCTTTACGCCTACTGGATCACGGTCAACTACCGCGAAGCCTTCGGCATGTACGCCTGCAACGGAATCCTCTTCAACCA
CGAGTCCCCGATCAGGGGAGAGACCTTCGTCACCAGGAAGATCACCCGCGCCATAGCCCGCATCTCCCTTGGCCTGCAGA
GCTGCCTCTACCTCGGCAACCTGAGCGCCCTGCGCGACTGGGGGCACGCCCGCGATTACGTCGAGATGCAATGGCTCATG
CTGCAGCAGGATGAGCCGGATGATTTCGTCATCGCCACGGGGAAGCAGTTTTCCGTCCGGCAGATGGTCGAAGAAGCGGC
CAAGGGGGTCGGCATCTGCATGCGCTGGGAAGGCGAAGGCGTCAACGAGACCGGCATCGTCGCGTCCATAGATAGCCACG
GCACCAAGACCGAGGGGGGCGCCGCCGGCCACCTGCAAGTGGGACAGGCCATCGTGAAAGTGGATCCTCGCTACTTCAGG
CCGACCGAAGTGGAGACCCTTTTGGGAGACCCGAGCAAGGCACGGCAGAAACTGGGGTGGGTTCCGAAGACCACGTTCGA
GGAACTGGTGGCCGAAATGGTGCACTCGGATCTGGAGGAAGCGCGTCGCGACGAGCTCTGCAAACATCACGGCTTCAGCA
CCTACAACTACCACGAGTAG

Upstream 100 bases:

>100_bases
ATCGGCTTCGACTGAGGCATCTCCCGCAGCGGTTTAAGCATGGCCGTGCGGCCGAGAACGGCTTCACGATCTTCGATGGA
ACAAAAGAGGAGATTGTTAC

Downstream 100 bases:

>100_bases
CAGCGAGGACCGTTAGTTCAAACATGAATACCAACGCCAAGATTTTTGTCGCAGGCTCCAAAGGGCTTGTCGGTTCCGCG
CTGACACGCAGCCTCAAATC

Product: bifunctional GDP-mannose 4,6-dehydratase/GDP-6-deoxy-D-lyxo-4-hexulose reductase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MAKVALITGVTGQDGAYLAEFLLKKGYVVHGIKRRASLFNTDRIDHLYQDPHIENRDFILHYGDLTDSTNLIRIIQQVQP
DEIYNLAAMSHVAVSFETPEYTANADGLGTLRILEAIRILGLEKKTRFYQASTSELYGLVQEVPQSEKTPFYPRSPYAVA
KLYAYWITVNYREAFGMYACNGILFNHESPIRGETFVTRKITRAIARISLGLQSCLYLGNLSALRDWGHARDYVEMQWLM
LQQDEPDDFVIATGKQFSVRQMVEEAAKGVGICMRWEGEGVNETGIVASIDSHGTKTEGGAAGHLQVGQAIVKVDPRYFR
PTEVETLLGDPSKARQKLGWVPKTTFEELVAEMVHSDLEEARRDELCKHHGFSTYNYHE

Sequences:

>Translated_379_residues
MAKVALITGVTGQDGAYLAEFLLKKGYVVHGIKRRASLFNTDRIDHLYQDPHIENRDFILHYGDLTDSTNLIRIIQQVQP
DEIYNLAAMSHVAVSFETPEYTANADGLGTLRILEAIRILGLEKKTRFYQASTSELYGLVQEVPQSEKTPFYPRSPYAVA
KLYAYWITVNYREAFGMYACNGILFNHESPIRGETFVTRKITRAIARISLGLQSCLYLGNLSALRDWGHARDYVEMQWLM
LQQDEPDDFVIATGKQFSVRQMVEEAAKGVGICMRWEGEGVNETGIVASIDSHGTKTEGGAAGHLQVGQAIVKVDPRYFR
PTEVETLLGDPSKARQKLGWVPKTTFEELVAEMVHSDLEEARRDELCKHHGFSTYNYHE
>Mature_378_residues
AKVALITGVTGQDGAYLAEFLLKKGYVVHGIKRRASLFNTDRIDHLYQDPHIENRDFILHYGDLTDSTNLIRIIQQVQPD
EIYNLAAMSHVAVSFETPEYTANADGLGTLRILEAIRILGLEKKTRFYQASTSELYGLVQEVPQSEKTPFYPRSPYAVAK
LYAYWITVNYREAFGMYACNGILFNHESPIRGETFVTRKITRAIARISLGLQSCLYLGNLSALRDWGHARDYVEMQWLML
QQDEPDDFVIATGKQFSVRQMVEEAAKGVGICMRWEGEGVNETGIVASIDSHGTKTEGGAAGHLQVGQAIVKVDPRYFRP
TEVETLLGDPSKARQKLGWVPKTTFEELVAEMVHSDLEEARRDELCKHHGFSTYNYHE

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=363, Percent_Identity=59.7796143250689, Blast_Score=447, Evalue=1e-126,
Organism=Escherichia coli, GI1788366, Length=372, Percent_Identity=72.3118279569892, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI48994969, Length=360, Percent_Identity=26.1111111111111, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1788353, Length=202, Percent_Identity=26.7326732673267, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17507723, Length=358, Percent_Identity=64.5251396648045, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17539424, Length=358, Percent_Identity=63.6871508379888, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI133901790, Length=358, Percent_Identity=63.6871508379888, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI133901788, Length=358, Percent_Identity=63.6871508379888, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17539422, Length=358, Percent_Identity=63.6871508379888, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI133901786, Length=358, Percent_Identity=63.6871508379888, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24158427, Length=367, Percent_Identity=59.4005449591281, Blast_Score=447, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 42819; Mature: 42688

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVALITGVTGQDGAYLAEFLLKKGYVVHGIKRRASLFNTDRIDHLYQDPHIENRDFIL
CCEEEEEECCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHCCHHHHHHHCCCCCCCCCEEE
HYGDLTDSTNLIRIIQQVQPDEIYNLAAMSHVAVSFETPEYTANADGLGTLRILEAIRIL
EECCCCCCHHHHHHHHHCCCHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHH
GLEKKTRFYQASTSELYGLVQEVPQSEKTPFYPRSPYAVAKLYAYWITVNYREAFGMYAC
CCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEEEEEEEHHHHHCCEEE
NGILFNHESPIRGETFVTRKITRAIARISLGLQSCLYLGNLSALRDWGHARDYVEMQWLM
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEE
LQQDEPDDFVIATGKQFSVRQMVEEAAKGVGICMRWEGEGVNETGIVASIDSHGTKTEGG
EECCCCCCEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCC
AAGHLQVGQAIVKVDPRYFRPTEVETLLGDPSKARQKLGWVPKTTFEELVAEMVHSDLEE
CCCCHHHCCEEEEECCCCCCCCCHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ARRDELCKHHGFSTYNYHE
HHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AKVALITGVTGQDGAYLAEFLLKKGYVVHGIKRRASLFNTDRIDHLYQDPHIENRDFIL
CEEEEEECCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHCCHHHHHHHCCCCCCCCCEEE
HYGDLTDSTNLIRIIQQVQPDEIYNLAAMSHVAVSFETPEYTANADGLGTLRILEAIRIL
EECCCCCCHHHHHHHHHCCCHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHH
GLEKKTRFYQASTSELYGLVQEVPQSEKTPFYPRSPYAVAKLYAYWITVNYREAFGMYAC
CCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEEEEEEEHHHHHCCEEE
NGILFNHESPIRGETFVTRKITRAIARISLGLQSCLYLGNLSALRDWGHARDYVEMQWLM
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEE
LQQDEPDDFVIATGKQFSVRQMVEEAAKGVGICMRWEGEGVNETGIVASIDSHGTKTEGG
EECCCCCCEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCC
AAGHLQVGQAIVKVDPRYFRPTEVETLLGDPSKARQKLGWVPKTTFEELVAEMVHSDLEE
CCCCHHHCCEEEEECCCCCCCCCHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ARRDELCKHHGFSTYNYHE
HHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]