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The map label for this gene is oprF [H]

Identifier: 197117521

GI number: 197117521

Start: 1308919

End: 1310271

Strand: Reverse

Name: oprF [H]

Synonym: Gbem_1132

Alternate gene names: 197117521

Gene position: 1310271-1308919 (Counterclockwise)

Preceding gene: 197117523

Following gene: 197117520

Centisome position: 28.39

GC content: 53.95

Gene sequence:

>1353_bases
ATGAATGCTATAACGCGCGTGTTCATTCCACCGGCACTGATTGCTTTGCTGCTGATTCTACACACGCAATGCCTAGCGGC
AGACAAATACGACAGTTTCACTACAACCCCTGTAGTAGGTATCTTCAGTCCAGGCAGCTTCAGTTCCCTCAAGCCTGGTT
TAATGGCAGGGGTTAAGATTGACAAACAGCTGACAGACACTTTTGGGATTGAGGGAGACATCAGCATGGTTGGTGCCGAC
GCAAAATCGTCAGGCAGCACAGCCCTATACCTAGTCACCTTACAGACTATTTATCCTTTTCTCGAACGCCCCAATTTAAC
CCCATTCTTGACGTTCGGTGCTGGCGGACTCATTGCTGACAAAGGGTCATCGAGCCCTTTAGTAAACTTCGGTCTCGGAG
CCAAGTACTTCATAAAAAAGGATGTTGCCTTCAGGTTTGATTTCCGGGATGTCGCCGCAAGCAGCCAGGGAAACAGTTTC
GAATTAACTGCGGGGGTAAGTTTCGTCTTCGGCGGAGAAAAGAAGCAGCCCCGCCACAGGGCGCGCCCACAGATTGCCGC
AGCGACACCTATGACGAATCCGGCGGCCACCGAGGCATCAACTAGCCTTTCGACACAAACGGCTAAAGACCAGCCTGTTA
TAACGCATAGGGAAACCCCTAAGCCAGTTCCCGCATCCCCCGCGCCTGCAACTGTTGCAACGACGCCGCAGAGTCTTGCC
GTTCCAGTGATCGCCACGCCCGGCATTGCCTCCTCTGCATCAAGCGTGCAGCCTCCCCCCGTGGCTGCAACTGTTGCAAC
GACGCCGCAGAGCCTTGCCGTTCCAGTGATCGTCACGCCCGGCATTGCCTCCTCTGCATCAAGCGTGCAGCCTCCCCCCG
TGGCTGCAGAAATTGCAAAAACGGCACCGGCGCCACGAGAGAACGACAGCACCGAAGCAACAGACCAGGCAAAACAAATA
GAACTGGAAGCTGAAAAAAAACCAGCGTCAACACCTTCCGTGCCAGTTGCCGCCCCGTTACTCAAAGCCACCTTCGAGTT
CGACTGCAACAGCTACAAAATCAGGCCGAGCTTCATCGATGTGGGGCGAAAAATCGGCCTGTTCATGAAGCGTAACCCAC
AGATCATTGCACAGATCCACGGACACGCCGATATCACCGGCAGCGATGACCACAACCTCGCGCTTTCAGAGAAACGCGCA
GTGCGAACAAAGCAGTACCTGATGAAGATGTACAATCTTTCTGCCGATCGGTTCGAAATCCGCACCATGGGCAGTTCCGA
ACCGATCGCAGATAACACGACAGAGGTCGGTCGCAAAAAAAACCGCCGGGCCCTCGTCATCATTTTAAAGTGA

Upstream 100 bases:

>100_bases
GCACTTGAGGTCATTAGGCATTACAACAAAGCAGCGCGGGATGCGTGATGCGGCGTTTCCACTGTGAGCCCAATACAATG
CCACGAAAAGGATTGCAATA

Downstream 100 bases:

>100_bases
CCCTTCCCCCCAATATGCCTTTTCAGAGACAGCCGTCCCCCTGCCTTCTCTGAAAAGGCACCTCTTCAGCCGGGGCAGTA
AATGGCCTGCTGCCCCGGCG

Product: OmpA family outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MNAITRVFIPPALIALLLILHTQCLAADKYDSFTTTPVVGIFSPGSFSSLKPGLMAGVKIDKQLTDTFGIEGDISMVGAD
AKSSGSTALYLVTLQTIYPFLERPNLTPFLTFGAGGLIADKGSSSPLVNFGLGAKYFIKKDVAFRFDFRDVAASSQGNSF
ELTAGVSFVFGGEKKQPRHRARPQIAAATPMTNPAATEASTSLSTQTAKDQPVITHRETPKPVPASPAPATVATTPQSLA
VPVIATPGIASSASSVQPPPVAATVATTPQSLAVPVIVTPGIASSASSVQPPPVAAEIAKTAPAPRENDSTEATDQAKQI
ELEAEKKPASTPSVPVAAPLLKATFEFDCNSYKIRPSFIDVGRKIGLFMKRNPQIIAQIHGHADITGSDDHNLALSEKRA
VRTKQYLMKMYNLSADRFEIRTMGSSEPIADNTTEVGRKKNRRALVIILK

Sequences:

>Translated_450_residues
MNAITRVFIPPALIALLLILHTQCLAADKYDSFTTTPVVGIFSPGSFSSLKPGLMAGVKIDKQLTDTFGIEGDISMVGAD
AKSSGSTALYLVTLQTIYPFLERPNLTPFLTFGAGGLIADKGSSSPLVNFGLGAKYFIKKDVAFRFDFRDVAASSQGNSF
ELTAGVSFVFGGEKKQPRHRARPQIAAATPMTNPAATEASTSLSTQTAKDQPVITHRETPKPVPASPAPATVATTPQSLA
VPVIATPGIASSASSVQPPPVAATVATTPQSLAVPVIVTPGIASSASSVQPPPVAAEIAKTAPAPRENDSTEATDQAKQI
ELEAEKKPASTPSVPVAAPLLKATFEFDCNSYKIRPSFIDVGRKIGLFMKRNPQIIAQIHGHADITGSDDHNLALSEKRA
VRTKQYLMKMYNLSADRFEIRTMGSSEPIADNTTEVGRKKNRRALVIILK
>Mature_450_residues
MNAITRVFIPPALIALLLILHTQCLAADKYDSFTTTPVVGIFSPGSFSSLKPGLMAGVKIDKQLTDTFGIEGDISMVGAD
AKSSGSTALYLVTLQTIYPFLERPNLTPFLTFGAGGLIADKGSSSPLVNFGLGAKYFIKKDVAFRFDFRDVAASSQGNSF
ELTAGVSFVFGGEKKQPRHRARPQIAAATPMTNPAATEASTSLSTQTAKDQPVITHRETPKPVPASPAPATVATTPQSLA
VPVIATPGIASSASSVQPPPVAATVATTPQSLAVPVIVTPGIASSASSVQPPPVAAEIAKTAPAPRENDSTEATDQAKQI
ELEAEKKPASTPSVPVAAPLLKATFEFDCNSYKIRPSFIDVGRKIGLFMKRNPQIIAQIHGHADITGSDDHNLALSEKRA
VRTKQYLMKMYNLSADRFEIRTMGSSEPIADNTTEVGRKKNRRALVIILK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 47638; Mature: 47638

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAITRVFIPPALIALLLILHTQCLAADKYDSFTTTPVVGIFSPGSFSSLKPGLMAGVKI
CCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCHHCCEEE
DKQLTDTFGIEGDISMVGADAKSSGSTALYLVTLQTIYPFLERPNLTPFLTFGAGGLIAD
CHHHHHHCCCCCCEEEEECCCCCCCCEEEEEEEHHHHHHHHHCCCCCEEEEECCCCEEEC
KGSSSPLVNFGLGAKYFIKKDVAFRFDFRDVAASSQGNSFELTAGVSFVFGGEKKQPRHR
CCCCCCEEEECCCCEEEEEECEEEEECHHHHHCCCCCCEEEEEECEEEEECCCCCCCHHH
ARPQIAAATPMTNPAATEASTSLSTQTAKDQPVITHRETPKPVPASPAPATVATTPQSLA
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEECCHHHE
VPVIATPGIASSASSVQPPPVAATVATTPQSLAVPVIVTPGIASSASSVQPPPVAAEIAK
EEEEECCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHH
TAPAPRENDSTEATDQAKQIELEAEKKPASTPSVPVAAPLLKATFEFDCNSYKIRPSFID
CCCCCCCCCCCCHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHEEEECCCCEEECHHHHH
VGRKIGLFMKRNPQIIAQIHGHADITGSDDHNLALSEKRAVRTKQYLMKMYNLSADRFEI
HCHHCCEEEECCCEEEEEEECCEECCCCCCCCEEECHHHHHHHHHHHHHHHCCCCCEEEE
RTMGSSEPIADNTTEVGRKKNRRALVIILK
EECCCCCCCCCCCHHHHHCCCCCEEEEEEC
>Mature Secondary Structure
MNAITRVFIPPALIALLLILHTQCLAADKYDSFTTTPVVGIFSPGSFSSLKPGLMAGVKI
CCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCHHCCEEE
DKQLTDTFGIEGDISMVGADAKSSGSTALYLVTLQTIYPFLERPNLTPFLTFGAGGLIAD
CHHHHHHCCCCCCEEEEECCCCCCCCEEEEEEEHHHHHHHHHCCCCCEEEEECCCCEEEC
KGSSSPLVNFGLGAKYFIKKDVAFRFDFRDVAASSQGNSFELTAGVSFVFGGEKKQPRHR
CCCCCCEEEECCCCEEEEEECEEEEECHHHHHCCCCCCEEEEEECEEEEECCCCCCCHHH
ARPQIAAATPMTNPAATEASTSLSTQTAKDQPVITHRETPKPVPASPAPATVATTPQSLA
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEECCHHHE
VPVIATPGIASSASSVQPPPVAATVATTPQSLAVPVIVTPGIASSASSVQPPPVAAEIAK
EEEEECCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHH
TAPAPRENDSTEATDQAKQIELEAEKKPASTPSVPVAAPLLKATFEFDCNSYKIRPSFID
CCCCCCCCCCCCHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHEEEECCCCEEECHHHHH
VGRKIGLFMKRNPQIIAQIHGHADITGSDDHNLALSEKRAVRTKQYLMKMYNLSADRFEI
HCHHCCEEEECCCEEEEEEECCEECCCCCCCCEEECHHHHHHHHHHHHHHHCCCCCEEEE
RTMGSSEPIADNTTEVGRKKNRRALVIILK
EECCCCCCCCCCCHHHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]