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The map label for this gene is pulE-3 [H]

Identifier: 197117294

GI number: 197117294

Start: 1062758

End: 1064545

Strand: Direct

Name: pulE-3 [H]

Synonym: Gbem_0904

Alternate gene names: 197117294

Gene position: 1062758-1064545 (Clockwise)

Preceding gene: 197117287

Following gene: 197117298

Centisome position: 23.03

GC content: 63.31

Gene sequence:

>1788_bases
ATGCACAGGAAGAAGATGTTCACCATTAAGAATGTGGGACAGATACTCCTGCAGCGCCAGATGATCAGCGAGGAGCAGTT
CCAGGTGCTGCTCGCCCAAGGTGAGGCGCAGGCCCAGCGGCTGGCCGGCGCGCAGCAGGCCGGCTATTCTCGCCGGGTGC
TGCATGCGCCGGAAAAGGCCTCTCCTGCCGAGGTGATCGCCTCCTTCAACCTGGAGATTCCGGGAGCGGGGGGGAGGCTT
CTGACCGAGGACGCCATCACGGAGGTGCTGGCACAGGCTGTCGGGCTTCCTTATCTCAAGATCAACCCGATGAAGCTCAA
TCTCGATCTGGTCACGGCGCACATCTCCCGCCCCTTCGCCTTGAAGCACCTCATCGTCCCGGTGGGATACGAGGAGGGGG
TGGTGGTGCTGGCGGTAGCCGACCCCTTCAACGAGGAGGTCGTCGAGGAGCTCAGATCCATCAAGCGGATGGAGTTCCGC
CGCGTCTTAGCCTCCAGAAACGACATCCTCAAGATACTCAGGGAGTTCTTCGGCTTCCGCGCCTCGGTGCAGGCCGCCGA
GAGCGAGGTAACGGCGGGGGTGGACCTCGGCAACCTCGAGCAGTTCGTGCGCATGAAGACCGGGCACGAGATCGAGGGAA
CGGACCGCAACATCATCTCCGCCGTGGATTTCCTCTTGCAGTACGCCTTCGACCAGCGCGCCAGCGACATCCACATCGAG
CCCAAGAGGGAGAAATCCCTGGTGCGGCTCAGGGTGGACGGCGTGCTGCACAACGTCCACGTGGTACCCAAGCAGCTGCA
CCCCCCCATCGTCTCCCGCATCAAGATGCTCTCCCGCATGGACCTCGCCGAAAAGAGGCGTCCCCAGGACGGCCGGATCA
AGACCAACCACCAGGAAAAGGAGGTTGAGCTGCGCGTCTCCACGCTCCCTGTCGCCTTCGGCGAGAAGGTGGTGATCAGG
ATCTTCGATCCCGACGTGCTGATGCAGGAGCTCGACCAGATAGGCTTCTATCCGCGGGAGTACCAGCTCTACAGCTCGTT
TTTGCGCAGGCCCAACGGCATCATCCTCGTCACCGGACCTACCGGCAGCGGCAAGACCACGACGCTCTACTCGTCCCTGC
GCACCCTCTCCTCGCCCGAGGTCAACATCGTCACCGTGGAGGACCCCATCGAGATGGTGATGGAGGAGTTCAACCAGGTG
GGGGTCCAGTCCGGGATCGGGGTGACCTTCGACAAGGTGTTGAGAAACGTGCTGAGGCAGGACCCGGACATCATCATGAT
CGGGGAGATCCGGGACAAGGAGACGGCCGAGAACGCGGTGCAGGCGGCGCTCACCGGGCACCTGGTGCTATCCACCCTGC
ACACGAACGACGCTCCCTCTTCGGTGACGAGGCTCATCGACCTGGGCGTCCCCCCCTTTCTCATCTCTTCCACCGTGGTC
GGGATCATCGCGCAGAGGCTGCTGCGCAAGATATGTCCGGCTTGCAAGAAGGAGCGCCGGCTTGCCCCCGACGAGCTCGA
ATACCTGGGGCTCAAGCGCGCCGGAACCGTCTGGTTCGGCGAGGGGTGCGCAGAGTGCCGCGGCACCGGCTACAAGGGGA
GGACCGGGATCTTCGAGGTGCTGGACGTGAACGAAGCGGTGAAGGGGGTTATTTCGGAGCGGCTCGACCTGGCCGAGTTG
CAGGCGGTCGCCCGCCGCGACGGCCTGGTCACGCTCAGGGAGCAGGCCATCCGCAAGATGCTGGAGGGGATCTCCACTTA
CGAAGAGGTGATTGCAGTCACGGGGTAG

Upstream 100 bases:

>100_bases
TATCGGTCGGCCGTCCATTGTCAATCTTGGACACCCTTGACATTATTAAGGTTCTCGCTGATACTCCCAGCTTTAAGATA
ATGAGAGAGGAACCGCACGC

Downstream 100 bases:

>100_bases
GGCGCTGGCCGGCCAGGGGTCGGACAGGCGCGGCTAGCTCTTTTTTTGCAGTGCCTGTTGCAGGTCCGCCCAACGGTACG
GCTTCACCACGGCGCAACAG

Product: type II secretion system ATPase PulE

Products: NA

Alternate protein names: Type II traffic warden ATPase [H]

Number of amino acids: Translated: 595; Mature: 595

Protein sequence:

>595_residues
MHRKKMFTIKNVGQILLQRQMISEEQFQVLLAQGEAQAQRLAGAQQAGYSRRVLHAPEKASPAEVIASFNLEIPGAGGRL
LTEDAITEVLAQAVGLPYLKINPMKLNLDLVTAHISRPFALKHLIVPVGYEEGVVVLAVADPFNEEVVEELRSIKRMEFR
RVLASRNDILKILREFFGFRASVQAAESEVTAGVDLGNLEQFVRMKTGHEIEGTDRNIISAVDFLLQYAFDQRASDIHIE
PKREKSLVRLRVDGVLHNVHVVPKQLHPPIVSRIKMLSRMDLAEKRRPQDGRIKTNHQEKEVELRVSTLPVAFGEKVVIR
IFDPDVLMQELDQIGFYPREYQLYSSFLRRPNGIILVTGPTGSGKTTTLYSSLRTLSSPEVNIVTVEDPIEMVMEEFNQV
GVQSGIGVTFDKVLRNVLRQDPDIIMIGEIRDKETAENAVQAALTGHLVLSTLHTNDAPSSVTRLIDLGVPPFLISSTVV
GIIAQRLLRKICPACKKERRLAPDELEYLGLKRAGTVWFGEGCAECRGTGYKGRTGIFEVLDVNEAVKGVISERLDLAEL
QAVARRDGLVTLREQAIRKMLEGISTYEEVIAVTG

Sequences:

>Translated_595_residues
MHRKKMFTIKNVGQILLQRQMISEEQFQVLLAQGEAQAQRLAGAQQAGYSRRVLHAPEKASPAEVIASFNLEIPGAGGRL
LTEDAITEVLAQAVGLPYLKINPMKLNLDLVTAHISRPFALKHLIVPVGYEEGVVVLAVADPFNEEVVEELRSIKRMEFR
RVLASRNDILKILREFFGFRASVQAAESEVTAGVDLGNLEQFVRMKTGHEIEGTDRNIISAVDFLLQYAFDQRASDIHIE
PKREKSLVRLRVDGVLHNVHVVPKQLHPPIVSRIKMLSRMDLAEKRRPQDGRIKTNHQEKEVELRVSTLPVAFGEKVVIR
IFDPDVLMQELDQIGFYPREYQLYSSFLRRPNGIILVTGPTGSGKTTTLYSSLRTLSSPEVNIVTVEDPIEMVMEEFNQV
GVQSGIGVTFDKVLRNVLRQDPDIIMIGEIRDKETAENAVQAALTGHLVLSTLHTNDAPSSVTRLIDLGVPPFLISSTVV
GIIAQRLLRKICPACKKERRLAPDELEYLGLKRAGTVWFGEGCAECRGTGYKGRTGIFEVLDVNEAVKGVISERLDLAEL
QAVARRDGLVTLREQAIRKMLEGISTYEEVIAVTG
>Mature_595_residues
MHRKKMFTIKNVGQILLQRQMISEEQFQVLLAQGEAQAQRLAGAQQAGYSRRVLHAPEKASPAEVIASFNLEIPGAGGRL
LTEDAITEVLAQAVGLPYLKINPMKLNLDLVTAHISRPFALKHLIVPVGYEEGVVVLAVADPFNEEVVEELRSIKRMEFR
RVLASRNDILKILREFFGFRASVQAAESEVTAGVDLGNLEQFVRMKTGHEIEGTDRNIISAVDFLLQYAFDQRASDIHIE
PKREKSLVRLRVDGVLHNVHVVPKQLHPPIVSRIKMLSRMDLAEKRRPQDGRIKTNHQEKEVELRVSTLPVAFGEKVVIR
IFDPDVLMQELDQIGFYPREYQLYSSFLRRPNGIILVTGPTGSGKTTTLYSSLRTLSSPEVNIVTVEDPIEMVMEEFNQV
GVQSGIGVTFDKVLRNVLRQDPDIIMIGEIRDKETAENAVQAALTGHLVLSTLHTNDAPSSVTRLIDLGVPPFLISSTVV
GIIAQRLLRKICPACKKERRLAPDELEYLGLKRAGTVWFGEGCAECRGTGYKGRTGIFEVLDVNEAVKGVISERLDLAEL
QAVARRDGLVTLREQAIRKMLEGISTYEEVIAVTG

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of a variety of enzymes across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=466, Percent_Identity=37.7682403433476, Blast_Score=328, Evalue=9e-91,
Organism=Escherichia coli, GI1786296, Length=373, Percent_Identity=43.4316353887399, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI87082188, Length=125, Percent_Identity=48, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 66326; Mature: 66326

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRKKMFTIKNVGQILLQRQMISEEQFQVLLAQGEAQAQRLAGAQQAGYSRRVLHAPEKA
CCCCCEEHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEECCCCCC
SPAEVIASFNLEIPGAGGRLLTEDAITEVLAQAVGLPYLKINPMKLNLDLVTAHISRPFA
CHHHHHHCCCEECCCCCCCEECHHHHHHHHHHHHCCCEEEECCEEEEHEEEEEHHCCCCE
LKHLIVPVGYEEGVVVLAVADPFNEEVVEELRSIKRMEFRRVLASRNDILKILREFFGFR
EHHEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCH
ASVQAAESEVTAGVDLGNLEQFVRMKTGHEIEGTDRNIISAVDFLLQYAFDQRASDIHIE
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
PKREKSLVRLRVDGVLHNVHVVPKQLHPPIVSRIKMLSRMDLAEKRRPQDGRIKTNHQEK
CCCCCCEEEEEECHHHHHHEECCHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
EVELRVSTLPVAFGEKVVIRIFDPDVLMQELDQIGFYPREYQLYSSFLRRPNGIILVTGP
EEEEEEEEECEECCCEEEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECC
TGSGKTTTLYSSLRTLSSPEVNIVTVEDPIEMVMEEFNQVGVQSGIGVTFDKVLRNVLRQ
CCCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHC
DPDIIMIGEIRDKETAENAVQAALTGHLVLSTLHTNDAPSSVTRLIDLGVPPFLISSTVV
CCCEEEEECCCCHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCCHHHHHHHHH
GIIAQRLLRKICPACKKERRLAPDELEYLGLKRAGTVWFGEGCAECRGTGYKGRTGIFEV
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEECCCHHHHCCCCCCCCCCHHHH
LDVNEAVKGVISERLDLAELQAVARRDGLVTLREQAIRKMLEGISTYEEVIAVTG
CCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHRKKMFTIKNVGQILLQRQMISEEQFQVLLAQGEAQAQRLAGAQQAGYSRRVLHAPEKA
CCCCCEEHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEECCCCCC
SPAEVIASFNLEIPGAGGRLLTEDAITEVLAQAVGLPYLKINPMKLNLDLVTAHISRPFA
CHHHHHHCCCEECCCCCCCEECHHHHHHHHHHHHCCCEEEECCEEEEHEEEEEHHCCCCE
LKHLIVPVGYEEGVVVLAVADPFNEEVVEELRSIKRMEFRRVLASRNDILKILREFFGFR
EHHEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCH
ASVQAAESEVTAGVDLGNLEQFVRMKTGHEIEGTDRNIISAVDFLLQYAFDQRASDIHIE
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
PKREKSLVRLRVDGVLHNVHVVPKQLHPPIVSRIKMLSRMDLAEKRRPQDGRIKTNHQEK
CCCCCCEEEEEECHHHHHHEECCHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
EVELRVSTLPVAFGEKVVIRIFDPDVLMQELDQIGFYPREYQLYSSFLRRPNGIILVTGP
EEEEEEEEECEECCCEEEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECC
TGSGKTTTLYSSLRTLSSPEVNIVTVEDPIEMVMEEFNQVGVQSGIGVTFDKVLRNVLRQ
CCCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHC
DPDIIMIGEIRDKETAENAVQAALTGHLVLSTLHTNDAPSSVTRLIDLGVPPFLISSTVV
CCCEEEEECCCCHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCCHHHHHHHHH
GIIAQRLLRKICPACKKERRLAPDELEYLGLKRAGTVWFGEGCAECRGTGYKGRTGIFEV
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEECCCHHHHCCCCCCCCCCHHHH
LDVNEAVKGVISERLDLAELQAVARRDGLVTLREQAIRKMLEGISTYEEVIAVTG
CCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1588814; 10984043 [H]