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The map label for this gene is 197117287

Identifier: 197117287

GI number: 197117287

Start: 1051482

End: 1053176

Strand: Direct

Name: 197117287

Synonym: Gbem_0897

Alternate gene names: NA

Gene position: 1051482-1053176 (Clockwise)

Preceding gene: 197117286

Following gene: 197117294

Centisome position: 22.78

GC content: 66.73

Gene sequence:

>1695_bases
TTGAAGATCCTCCTTGCGTACAAATGCCATCCCGAAGGCGCTGAAGACCCTTTCACCTCCCTGTTGCCGGCAGGGCTTCT
CTCCCTGCATGCCGTGCTGCTCAAAGCCGGGCATCAGGTCACCCTGGCCAACCTTTCCGGCTTCACTTGGGGCGAGGTCC
GCGCGCTTTTCAAGCGGCTGCGCCCCGAGGTCCTGGGGGTGTCGCAGTTCACCCACAACCGGGTGGAGTCGCTGCGCCTG
GCCGCTCTTGCCAAGGAGCTCGACCCGGGCTGCCTGGTGGCGCTCGGGGGGCCGCACGCGACCCATTGCTGGCGCGAGCT
CCTGGAGGGGCACCCCGAAGTCGACCTGGTGGTTCTCGGCGAGGGTGAGGCGACTGTTTGCGAACTGGTTGAGGCGAAGG
CTCTCGGCACACCCTGGGACAAGGTCGCCGGGCTCGCTTTCCGCGCCGCAGGAAAAGCAAGCCTCAGCGCGCCGCGAGCG
CCCATTACCGACCTGGACCTCCTGCCTCTGCCGGGAGCGGCCGAAGGGGACAGCATAGGGGTCGATTTCCGGCGTCAGCT
GGAGTTCGTCATCACCTCCCGCGGCTGCCCCGCCAGTTGCCTCTTCTGCTCCTCACCGCTCTTTTGGGGGCGCGGCGTCC
GCTTTCGGTCACCCGAGTCGGTGGTGCAAGAGATCCGCTCCTTGAAGGAGCGCTACGGGCTGATCTACTTCTCCTTCCGC
GACGATACCTTCACGGCCAACCGGGGGCGGGTGCTGGAGATCTGCCGCCTGCTCCGGCAGGAGCGGCTCCACGTCATGTG
GAACTGCCAGTCCCGGGTGAACGCAGTGGACGAGGAGATGCTCATCGCCATGAAGCAAGCGGGCTGCGAATGCATCCAGT
TCGGCGTGGAGTCGGGATCGCCGCAGATGCTGAAAGCACTCGGCAAGAGGATCCTTCCCCCTGAAGTGGAGCGTGCAGCC
GCGGCGGTGCGGCGCGCGGGCATCAACCTGTCGGTATACCTCATCACGGGGATTCCGGGGGAAGGGGAGGAGGACCTGCG
GCAGACGGTGCGGCTTATCGAGAGCATCCGCCCGCACGACGGGCAGGTCTCGCCGCTGGTGTACTACCCGGGTACCGAGC
TTTTGGCCCGCGCGGTGCGGGAAGAGGGGGTGCCGTCCGACCTCTTCGAGGAGGAGCGTGGCGAAGGGTTCCTGGTGCGC
CGCGACCCGTTCGTGGAGCGGTCGCGCCAGGCGATGCTGAAGGCGCTGGAGAAGGCGGGACGCAAAGCCGTGTTCACACA
GGCTTCGTATGCGGCGCAGCGAGCCCTTTTGGGGTACTGCTTCGTGACCGAGATGCTTTGTGCGGAATCCCTGGAGCAGC
AAGGGGACTGGGACGGGGCGCTCAGGTTATATCGCGGCATGGTGAAGGCCGAGCCCGACAACCCCTGGGGGTTGCTCCAA
CTCGGTGGATTGCAGGCGCGGATGGGGGATATGAAGGGGGCTTCCCGCTCCTACCAAAAGGCCCTGCAGCTGGTGCCGCG
GCACCTCCCCGCGTTGCTGGCATTGGGAGAGCTGGCCCTGGCGCAGGGTGATCGGCCCGGGACTGCCCGTCACTTCGCCG
AAGCGCTCAAGCTCGACCCCGCCGATCCCGTCGCGCTGGAAGGCGCGGCCGCAGCCGGCGCTGGCAGGGAGCAAAAGGGC
AAGCGCGGGAAATAA

Upstream 100 bases:

>100_bases
GAAAACGACTCGCTCGGGTTGACGAGCAGCAACTAGGCTAAACAGAACCACAGAACTTCCGAATCCGGCCCTCAGGGCCG
GATTTTTTTATGGGTGCAGC

Downstream 100 bases:

>100_bases
AAAAGGCGCCTTCCATCTAGGAGGGCGCCTTTTTTTATCACTGCTGCAGGGAAGCCTACTTGTTGACGGCGAGGCCCGGC
GCGAAGATGATTTTGTCGAA

Product: cobalamin-binding radical SAM domain-containing iron-sulfur cluster-binding oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MKILLAYKCHPEGAEDPFTSLLPAGLLSLHAVLLKAGHQVTLANLSGFTWGEVRALFKRLRPEVLGVSQFTHNRVESLRL
AALAKELDPGCLVALGGPHATHCWRELLEGHPEVDLVVLGEGEATVCELVEAKALGTPWDKVAGLAFRAAGKASLSAPRA
PITDLDLLPLPGAAEGDSIGVDFRRQLEFVITSRGCPASCLFCSSPLFWGRGVRFRSPESVVQEIRSLKERYGLIYFSFR
DDTFTANRGRVLEICRLLRQERLHVMWNCQSRVNAVDEEMLIAMKQAGCECIQFGVESGSPQMLKALGKRILPPEVERAA
AAVRRAGINLSVYLITGIPGEGEEDLRQTVRLIESIRPHDGQVSPLVYYPGTELLARAVREEGVPSDLFEEERGEGFLVR
RDPFVERSRQAMLKALEKAGRKAVFTQASYAAQRALLGYCFVTEMLCAESLEQQGDWDGALRLYRGMVKAEPDNPWGLLQ
LGGLQARMGDMKGASRSYQKALQLVPRHLPALLALGELALAQGDRPGTARHFAEALKLDPADPVALEGAAAAGAGREQKG
KRGK

Sequences:

>Translated_564_residues
MKILLAYKCHPEGAEDPFTSLLPAGLLSLHAVLLKAGHQVTLANLSGFTWGEVRALFKRLRPEVLGVSQFTHNRVESLRL
AALAKELDPGCLVALGGPHATHCWRELLEGHPEVDLVVLGEGEATVCELVEAKALGTPWDKVAGLAFRAAGKASLSAPRA
PITDLDLLPLPGAAEGDSIGVDFRRQLEFVITSRGCPASCLFCSSPLFWGRGVRFRSPESVVQEIRSLKERYGLIYFSFR
DDTFTANRGRVLEICRLLRQERLHVMWNCQSRVNAVDEEMLIAMKQAGCECIQFGVESGSPQMLKALGKRILPPEVERAA
AAVRRAGINLSVYLITGIPGEGEEDLRQTVRLIESIRPHDGQVSPLVYYPGTELLARAVREEGVPSDLFEEERGEGFLVR
RDPFVERSRQAMLKALEKAGRKAVFTQASYAAQRALLGYCFVTEMLCAESLEQQGDWDGALRLYRGMVKAEPDNPWGLLQ
LGGLQARMGDMKGASRSYQKALQLVPRHLPALLALGELALAQGDRPGTARHFAEALKLDPADPVALEGAAAAGAGREQKG
KRGK
>Mature_564_residues
MKILLAYKCHPEGAEDPFTSLLPAGLLSLHAVLLKAGHQVTLANLSGFTWGEVRALFKRLRPEVLGVSQFTHNRVESLRL
AALAKELDPGCLVALGGPHATHCWRELLEGHPEVDLVVLGEGEATVCELVEAKALGTPWDKVAGLAFRAAGKASLSAPRA
PITDLDLLPLPGAAEGDSIGVDFRRQLEFVITSRGCPASCLFCSSPLFWGRGVRFRSPESVVQEIRSLKERYGLIYFSFR
DDTFTANRGRVLEICRLLRQERLHVMWNCQSRVNAVDEEMLIAMKQAGCECIQFGVESGSPQMLKALGKRILPPEVERAA
AAVRRAGINLSVYLITGIPGEGEEDLRQTVRLIESIRPHDGQVSPLVYYPGTELLARAVREEGVPSDLFEEERGEGFLVR
RDPFVERSRQAMLKALEKAGRKAVFTQASYAAQRALLGYCFVTEMLCAESLEQQGDWDGALRLYRGMVKAEPDNPWGLLQ
LGGLQARMGDMKGASRSYQKALQLVPRHLPALLALGELALAQGDRPGTARHFAEALKLDPADPVALEGAAAAGAGREQKG
KRGK

Specific function: Unknown

COG id: COG1032

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02310 B12-binding; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 61640; Mature: 61640

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS50005 TPR L=RR ; PS50293 TPR_REGION ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILLAYKCHPEGAEDPFTSLLPAGLLSLHAVLLKAGHQVTLANLSGFTWGEVRALFKRL
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHH
RPEVLGVSQFTHNRVESLRLAALAKELDPGCLVALGGPHATHCWRELLEGHPEVDLVVLG
CHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCEEEEEEC
EGEATVCELVEAKALGTPWDKVAGLAFRAAGKASLSAPRAPITDLDLLPLPGAAEGDSIG
CCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VDFRRQLEFVITSRGCPASCLFCSSPLFWGRGVRFRSPESVVQEIRSLKERYGLIYFSFR
CCHHHHEEEHEECCCCCCHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEC
DDTFTANRGRVLEICRLLRQERLHVMWNCQSRVNAVDEEMLIAMKQAGCECIQFGVESGS
CCEEECCCCCHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCC
PQMLKALGKRILPPEVERAAAAVRRAGINLSVYLITGIPGEGEEDLRQTVRLIESIRPHD
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCC
GQVSPLVYYPGTELLARAVREEGVPSDLFEEERGEGFLVRRDPFVERSRQAMLKALEKAG
CCCCCEEECCCHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCC
RKAVFTQASYAAQRALLGYCFVTEMLCAESLEQQGDWDGALRLYRGMVKAEPDNPWGLLQ
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEE
LGGLQARMGDMKGASRSYQKALQLVPRHLPALLALGELALAQGDRPGTARHFAEALKLDP
ECCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
ADPVALEGAAAAGAGREQKGKRGK
CCCCEECCCHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKILLAYKCHPEGAEDPFTSLLPAGLLSLHAVLLKAGHQVTLANLSGFTWGEVRALFKRL
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHH
RPEVLGVSQFTHNRVESLRLAALAKELDPGCLVALGGPHATHCWRELLEGHPEVDLVVLG
CHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCEEEEEEC
EGEATVCELVEAKALGTPWDKVAGLAFRAAGKASLSAPRAPITDLDLLPLPGAAEGDSIG
CCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VDFRRQLEFVITSRGCPASCLFCSSPLFWGRGVRFRSPESVVQEIRSLKERYGLIYFSFR
CCHHHHEEEHEECCCCCCHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEC
DDTFTANRGRVLEICRLLRQERLHVMWNCQSRVNAVDEEMLIAMKQAGCECIQFGVESGS
CCEEECCCCCHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCC
PQMLKALGKRILPPEVERAAAAVRRAGINLSVYLITGIPGEGEEDLRQTVRLIESIRPHD
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCC
GQVSPLVYYPGTELLARAVREEGVPSDLFEEERGEGFLVRRDPFVERSRQAMLKALEKAG
CCCCCEEECCCHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCC
RKAVFTQASYAAQRALLGYCFVTEMLCAESLEQQGDWDGALRLYRGMVKAEPDNPWGLLQ
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEE
LGGLQARMGDMKGASRSYQKALQLVPRHLPALLALGELALAQGDRPGTARHFAEALKLDP
ECCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
ADPVALEGAAAAGAGREQKGKRGK
CCCCEECCCHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]