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The map label for this gene is leuB [H]

Identifier: 197117298

GI number: 197117298

Start: 1069867

End: 1070955

Strand: Direct

Name: leuB [H]

Synonym: Gbem_0908

Alternate gene names: 197117298

Gene position: 1069867-1070955 (Clockwise)

Preceding gene: 197117294

Following gene: 197117299

Centisome position: 23.18

GC content: 62.53

Gene sequence:

>1089_bases
ATGGGAAAGCTTTTTAAAGTGGCGGTATTGCCAGGAGACGGCATAGGTCCCGAGGTTATGGCTGAAGCACTGAGGGTGCT
CGATGCAGTCGAGAAACGTTACGATGTCACTTTCGAGCGGACCCACGCCAACGTAGGCGGGGCGGGAATCGACCAGGAAG
GTCGTGCGCTTCCCGAGACCACCGTAAACATATGCAAGGCTTCGGACGCCATCCTTTTCGGCTCCGTCGGCGGACCCAAG
TGGGAAACACTTCCCCCGGACGAGCAGCCCGAGCGCGGCGCCCTGCTGCCGCTTCGGAAGATCTTCGGCCTTTACGCCAA
CCTGCGTCCGGCCATCATCTTCCCGTCGCTCACCAGCGCCTCCTCGCTGAAGGAAGAGGTCATCGCAGGAGGCTTCGACA
TCCTGGTGATCCGCGAGCTGACCGGCGGCATCTACTTCTCCCAGCCCAAAGGGATCGAAGGCGAGGGGCGTAACCGCGTC
GGCGTCGACACCATGCGCTACAGCGTCCCGGAGATCGAGCGCATCGCGCACGTAGCATTCCAGGCGGCGAGAAAGCGCGG
CAAGAAGGTCTGCTCCATCGACAAGGCCAACGTCCTTTCCAGCTCCGTCCTCTGGCGCGAGATCGTGATCAACATCGCCA
AGGAATACCCGGATGTCGAGCTCTCCCACATGTACGTCGACAACGCCGCGATGCAGCTCGTGAAGTGGCCCAAGCAGTTC
GACGTGATCCTTTGCGAGAACATGTTCGGCGACATCCTCTCCGACGAGGCGGCCATGCTGACCGGCTCTCTGGGGATGCT
TCCCTCCGCCTCCCTGGCCGAGGGGACCTTCGGCATGTACGAGCCTTCCGGCGGGAGCGCCCCGGACATCGCAGGCCAGG
GGATCGCCAACCCGATCGCCCAGATCCTCTCGGCGGGGATGATGCTCCGTTACTCCTTCGGCATGATCGAGGCGGCCGAC
GCCATCGACAAGGCGGTCGCCAAGGTGCTGGACGGCGGGTACCGCACCAGGGACATCTATCAGGAGAAGGCAGGCGAGAA
GCTGGTGAACACCAAGGAGATCGGCGACGCCATCATCGCCAACCTCTAA

Upstream 100 bases:

>100_bases
TTTGGGTCGCTAAACGTATGTTTGACGGGCTTTTTTATGTTGACAATAGCGGCCGGTCGCAAATAGAATCGCTAATTTAA
TACATCTGGGAGGTCATGTG

Downstream 100 bases:

>100_bases
TTCAAGGGGACAGATTCAGGGGGACAGGCACCTGGCGGAGCCAGTCCCCTCGACGCGCAACACGGTAGAGGCGGGACTCT
CCCGCCGGTGCATATATTTA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MGKLFKVAVLPGDGIGPEVMAEALRVLDAVEKRYDVTFERTHANVGGAGIDQEGRALPETTVNICKASDAILFGSVGGPK
WETLPPDEQPERGALLPLRKIFGLYANLRPAIIFPSLTSASSLKEEVIAGGFDILVIRELTGGIYFSQPKGIEGEGRNRV
GVDTMRYSVPEIERIAHVAFQAARKRGKKVCSIDKANVLSSSVLWREIVINIAKEYPDVELSHMYVDNAAMQLVKWPKQF
DVILCENMFGDILSDEAAMLTGSLGMLPSASLAEGTFGMYEPSGGSAPDIAGQGIANPIAQILSAGMMLRYSFGMIEAAD
AIDKAVAKVLDGGYRTRDIYQEKAGEKLVNTKEIGDAIIANL

Sequences:

>Translated_362_residues
MGKLFKVAVLPGDGIGPEVMAEALRVLDAVEKRYDVTFERTHANVGGAGIDQEGRALPETTVNICKASDAILFGSVGGPK
WETLPPDEQPERGALLPLRKIFGLYANLRPAIIFPSLTSASSLKEEVIAGGFDILVIRELTGGIYFSQPKGIEGEGRNRV
GVDTMRYSVPEIERIAHVAFQAARKRGKKVCSIDKANVLSSSVLWREIVINIAKEYPDVELSHMYVDNAAMQLVKWPKQF
DVILCENMFGDILSDEAAMLTGSLGMLPSASLAEGTFGMYEPSGGSAPDIAGQGIANPIAQILSAGMMLRYSFGMIEAAD
AIDKAVAKVLDGGYRTRDIYQEKAGEKLVNTKEIGDAIIANL
>Mature_361_residues
GKLFKVAVLPGDGIGPEVMAEALRVLDAVEKRYDVTFERTHANVGGAGIDQEGRALPETTVNICKASDAILFGSVGGPKW
ETLPPDEQPERGALLPLRKIFGLYANLRPAIIFPSLTSASSLKEEVIAGGFDILVIRELTGGIYFSQPKGIEGEGRNRVG
VDTMRYSVPEIERIAHVAFQAARKRGKKVCSIDKANVLSSSVLWREIVINIAKEYPDVELSHMYVDNAAMQLVKWPKQFD
VILCENMFGDILSDEAAMLTGSLGMLPSASLAEGTFGMYEPSGGSAPDIAGQGIANPIAQILSAGMMLRYSFGMIEAADA
IDKAVAKVLDGGYRTRDIYQEKAGEKLVNTKEIGDAIIANL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=347, Percent_Identity=33.7175792507205, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI28178821, Length=343, Percent_Identity=29.4460641399417, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI4758582, Length=343, Percent_Identity=29.7376093294461, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI28178816, Length=315, Percent_Identity=30.1587301587302, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI28178838, Length=334, Percent_Identity=29.3413173652695, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI28178819, Length=171, Percent_Identity=35.0877192982456, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI87081683, Length=358, Percent_Identity=62.2905027932961, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI1788101, Length=368, Percent_Identity=34.2391304347826, Blast_Score=181, Evalue=5e-47,
Organism=Escherichia coli, GI1787381, Length=406, Percent_Identity=25.3694581280788, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71986051, Length=347, Percent_Identity=35.4466858789625, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI25144293, Length=360, Percent_Identity=34.4444444444444, Blast_Score=137, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17505779, Length=354, Percent_Identity=30.5084745762712, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17550882, Length=350, Percent_Identity=28.8571428571429, Blast_Score=118, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=364, Percent_Identity=46.1538461538462, Blast_Score=273, Evalue=3e-74,
Organism=Saccharomyces cerevisiae, GI6322097, Length=362, Percent_Identity=35.3591160220994, Blast_Score=166, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6324291, Length=347, Percent_Identity=33.4293948126801, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6324709, Length=338, Percent_Identity=31.6568047337278, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24643268, Length=340, Percent_Identity=35, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24643270, Length=340, Percent_Identity=35, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24661184, Length=367, Percent_Identity=32.9700272479564, Blast_Score=138, Evalue=5e-33,
Organism=Drosophila melanogaster, GI161078635, Length=347, Percent_Identity=29.1066282420749, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI161078637, Length=347, Percent_Identity=29.1066282420749, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI161078633, Length=346, Percent_Identity=29.4797687861272, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24650122, Length=346, Percent_Identity=29.4797687861272, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI161078639, Length=340, Percent_Identity=29.1176470588235, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281362242, Length=360, Percent_Identity=27.5, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648872, Length=360, Percent_Identity=27.5, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI20130355, Length=345, Percent_Identity=26.0869565217391, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38994; Mature: 38863

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLFKVAVLPGDGIGPEVMAEALRVLDAVEKRYDVTFERTHANVGGAGIDQEGRALPET
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHH
TVNICKASDAILFGSVGGPKWETLPPDEQPERGALLPLRKIFGLYANLRPAIIFPSLTSA
HHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCH
SSLKEEVIAGGFDILVIRELTGGIYFSQPKGIEGEGRNRVGVDTMRYSVPEIERIAHVAF
HHHHHHHHHCCCCEEEEEEHHCCEEECCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHH
QAARKRGKKVCSIDKANVLSSSVLWREIVINIAKEYPDVELSHMYVDNAAMQLVKWPKQF
HHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHEEHHHHHHHHHHCCHHH
DVILCENMFGDILSDEAAMLTGSLGMLPSASLAEGTFGMYEPSGGSAPDIAGQGIANPIA
CEEEHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
QILSAGMMLRYSFGMIEAADAIDKAVAKVLDGGYRTRDIYQEKAGEKLVNTKEIGDAIIA
HHHHCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NL
CC
>Mature Secondary Structure 
GKLFKVAVLPGDGIGPEVMAEALRVLDAVEKRYDVTFERTHANVGGAGIDQEGRALPET
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHH
TVNICKASDAILFGSVGGPKWETLPPDEQPERGALLPLRKIFGLYANLRPAIIFPSLTSA
HHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCH
SSLKEEVIAGGFDILVIRELTGGIYFSQPKGIEGEGRNRVGVDTMRYSVPEIERIAHVAF
HHHHHHHHHCCCCEEEEEEHHCCEEECCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHH
QAARKRGKKVCSIDKANVLSSSVLWREIVINIAKEYPDVELSHMYVDNAAMQLVKWPKQF
HHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHEEHHHHHHHHHHCCHHH
DVILCENMFGDILSDEAAMLTGSLGMLPSASLAEGTFGMYEPSGGSAPDIAGQGIANPIA
CEEEHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
QILSAGMMLRYSFGMIEAADAIDKAVAKVLDGGYRTRDIYQEKAGEKLVNTKEIGDAIIA
HHHHCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NL
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA